Contents Contributors Preface 1 [An Introduction to Freeze Fracture and Deep Et David M. Shotton and Nicholas J. Severs A Practical Introduction to Rapid Freezing Techniques Nicholas J. Severs, Terence M. Newman, and David M, Shotton A Guide to Equipment for Production of Freeze-Fracture Replicas Terence M. Nevenan Freeze-Fracture Artifacts: How to Recognize and Avoid Them Rkma) M, Abeysekera and Anthony W. Robards High Pressure Freezing John Z. Kiss and L, Andrew Staehelin Time-Resolved Analysis of Rapid Events Gerd Knoll Grid Mapped Freeze Fracture: Correlative Confocal Laser Scanning Microscopy and Freeze-fracture Electron Microscopy of Preselected Cells in Tissue Slices John E. Rash, Rebert K. Dillman. Michio Morita, L. Ray Whalen, Peter B. Guthrie, Denise Fay-Guthrie and David W. Wheeler Manipulation of Cell Monolayers to Reveal Plamz Membrane Surfaces for Freeze-Drying and Surface Replication Milan ¥. Nermut Freeze-Fracture Cytochemistry: An Explanatory Survey of Methods Nicholas J. Severs a 51 6 89 105 127 151 17310 an a a “ 16 v7 CONTENTS Backscattered Electron Imaging of Colloidal Gold-Labeled Fracture-Label reparations Frederick W. K. Kan Lipid Localization by Membrane Pesturbation: A Cautionary Tale Nicholas J. Severs Membrane Lipids and Model Membrane Systems ‘Michael J. Hope and Wendi ¥. Rodrigueza Freeze Fescture and Freeze Etching in Neurobiology: An Ongoing Story David W. Pumplin Events at the Cell Surface: Exocytosis and Remodeling of the [Extracellular Matrix as Seen in Quick-Frozen, Deep-Eiched Cells Nancy M. Mozingo and Douglas €. Chandler Visualization of Basement Membrane Molecules and Supramolecular Assemblies Peter D. Yurchenco and George C. Ruben Early Events in Atherosclerosis Captured by the Quick-Freeze! Deep-Etch Technique Joy § Frank, Giuliano Mottin, and Potrcia Nievestin-Post Viral Infection Studied by Freeze-Fracture Cytochemistry Maris Rosaria Tortst and Antonio Pavan Index 208 2as 238 255 285 at 335 347 361