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Point of Care Vascular Ultrasound of Fistulas and
Point of Care Vascular Ultrasound of Fistulas and
Point-of-care ultrasound (POCUS) is increasingly being used in nephrology as a diagnostic tool, and there is a growing interest
among physicians and nursing staff to learn how to use POCUS for the evaluation of the dialysis vascular access (DVA). The goal
of POCUS is to extend the physical examination and more closely evaluate the DVA at bedside. Typically, POCUS quickly
answers yes-no questions (ie, Is the vein too deep? Y/N). It is not the goal of POCUS of the vascular access to extensively
investigate the entire fistula or graft. In conjunction with a good physical examination, brightness-mode ultrasound alone
can answer most questions regarding the DVA, such as depth and diameter of the vessel. With some additional training, a
limited color Doppler can be added to the standard evaluation to check flow direction and pseudoaneurysms. With more
extensive training and an understanding of Doppler physics, access flow volumes can also be determined using spectral
Doppler-mode ultrasound.
Q 2021 by the National Kidney Foundation, Inc. All rights reserved.
Key Words: Ultrasound, Doppler ultrasound, Point-of-care, Dialysis vascular access, Arteriovenous fistula
the visibly used needle-insertion segment. The “stick spectral Doppler may not be available on hand-held
zone” can also be described as the body of the access and devices. In addition, the Doppler feature needs to include
has an arterial needle and venous needle insertion area. flow volume calculation software.
The venous outflow encompasses peripheral and central
veins leading to the right atrium. Training in Access Ultrasound
POCUS evaluation of the access is focused on the As with any technical skill, adequate training and prac-
“stick-zone” and determines depth, diameter, and the tice are a prerequisite. It is key to establish a compre-
location of side branches. Flow determination requires hensive curriculum and enforce rigorous training
an additional evaluation of the inflow artery or a common criteria for the POCUS novice to ensure the resulting
single outflow vein segment. benefits for patients. This would include didactic
training in principles of ultrasound and hands-on prac-
Physical Examination tice performing the particular examination. In addition,
The final step before performing a POCUS is a standard- knowledge and application of standardized documen-
ized physical examination of the DVA.7 This standardized tation, image storage, and report writing are equally
physical examination described in the literature includes important. A total of 50-100 training studies are often
evaluating the following 5 criteria: thrill, bruit, pulsatility, necessary to allow for competent and independent ul-
augmentation, and the collapse of the vein during arm trasound evaluation of the DVA. These studies should
elevation.8 The mastering of this comprehensive physical include B-Mode images of the body of the access with
examination enables the understanding of flow, pressure, measurement of depth and diameter. They should
and difficulties that could arise during needle cannulation. note if side-branches/hematomas are present and
Table 2 shows the 5 key elements of the physical should measure access flow volumes in the brachial
examination with a description of a normal and abnormal artery.
examination. The absence of To date, most training
a bruit and thrill will typi- programs for vascular tech-
CLINICAL SUMMARY
cally be sufficient to deter- nologist apply criteria of
mine that an access is the American Institute of
Point-of-care ultrasound for the nephrologist should
completely thrombosed. Ultrasound in Medicine.4
include dialysis vascular access (DVA) evaluation.
Increased pulsatility, with Currently, the American
no collapse of the body of Training includes mastering the correct nomenclature of
Society of Diagnostic and
the access, indicates a likely the DVA and proper physical examination technique.
Interventional Nephrology
outflow obstruction. Poor Brightness-mode (with black and white images) ultrasound is taking the lead in outlining
augmentation with absent allows for evaluation of depth and diameter of the vessel as criteria for vascular access
pulsatility indicates a likely well as evaluation for extravasations and can be a guide for ultrasound training and
inflow obstruction. Different cannulation of the access. certification in the use of
types of accesses are predis- ultrasound throughout
Spectral Doppler-mode, although requiring more training
posed to certain locations of and sufficient understanding of ultrasound physics, en- nephrology. These certifica-
stenoses as upper arm access ables reliable flow volume measurements which adds tion guidelines are presently
and grafts often have essential information to the overall DVA evaluation. under development.
outflow obstructions and
forearm fistulas most often
have inflow problems.9 ACCESS ULTRASOUND
In short, outflow stenoses will increase the intra-access Ultrasound evaluation has been shown to be helpful in
pressure with increased bleeding propensity, infiltrations, determining clinical maturation of the DVA while looking
and high venous pressure alarms while on the dialysis at diameter, depth, and flow volumes at 2 and 6 weeks
machine. Inflow stenoses are associated with difficult after a newly created access.10-12 A DVA is considered
needle placement, arterial needle alarms, or collapse of usable if the body of the access has a diameter of 4-6 mm
the access around the venous needle. Inflow and outflow and a depth of less than 5-6 mm, and the access has a
stenoses may decrease the overall access flow; therefore, flow volume of .500-600 mL/min.10,13 In our experience,
measurement of flow volume alone does not allow one the rule of 6 (,6 mm depth, .6 mm diameter, and
to determine where a stenosis is located. .600 mL/min flow) for defining access maturation is
easier to remember and obtains better results in dialysis
What Instruments Are Needed? units.
Ultrasound machines of any size, equipped with a To date, vascular laboratories or radiology departments
high-frequency probe (7.5-12 MHz) are required. have been responsible for performing maturation studies
Brightness-mode (B-Mode) ultrasound is standard on of the DVA, but with proper training, nephrology
any machine including hand-held ultrasound devices. practitioners can obtain the same parameters at the
For the utilization of Doppler, one has to differentiate bedside or in the office using POCUS. It is important to
between color Doppler and spectral Doppler. While color note that several additional questions can also be
Doppler is available on nearly all types of machines, answered using ultrasound at the bedside. Table 3 lists
Abbreviation: RADAR ¼ radial artery deviation and reimplantation (surgical technique for radial-cephalic autogenous access creation).
many of the questions, which may be addressed with determine if an access vein or graft is accessible with
POCUS applications. needles. If the vessel in the body of the access is at more
than 5- to 6-mm depth, cannulation will be difficult or
Technical Aspects of Performing POCUS of DVA impossible. Figure 3 demonstrates a vessel that is sufficient
Rest the patient’s arm comfortably on a pillow. Before in size and at appropriate depth for cannulation. If the
beginning any DVA ultrasound study, it is of utmost vessel is not 5- to 6-mm wide, it will be difficult to
importance to apply a liberal amount of ultrasound gel to cannulate without significant trauma and usually
the region of interest. Without adequate gel, the trans- indicates that the access does not have the right flow and
mission of ultrasound waves, and therefore the image pressure balance.
acquisition, will be incomplete. In addition, it is important Tributary veins, known as side branches, can divert flow
not to apply too much pressure with the ultrasound probe. from the main access vein and can be a cause for poor
augmentation observed during the physical examination.
B-Mode Ultrasound Side branches can occur in the presence and in the absence
POCUS with B-Mode allows for a visual map of the DVA. It of stenoses of the access vein. In this situation, side
helps identify straight and curved sections. A transverse branches can be located by performing a transverse sweep
and longitudinal evaluation with B-Mode helps to (sweeping of the ultrasound probe from the inflow to the
Figure 1. Images of the most frequent types of accesses. (A) Left forearm radial-cephalic autogenous access. (B) Left upper
arm brachial-cephalic autogenous access. (C) Right upper arm transposed brachial-basilic autogenous access. (D) Left
forearm brachial-cephalic loop prosthetic access. (E) Left upper arm brachial axillary loop prosthetic access.
Adv Chronic Kidney Dis. 2021;28(3):227-235
230 Voiculescu and Hentschel
Table 2. Physical Examination Components for Normal and Dysfunctional Dialysis Accesses
Exam Component Normal Outflow Stenosis Inflow Stenosis
Thrill Continuous (juxta-anastomotic) Continuous (@stenosis) Discontinuous*
Discontinuous* (juxta-anastomotic)
(juxta-anastomotic)
Bruit Continuous (inflow/body) Continuous/Discontinuous* Continuous/Discontinuous*
High pitch @stenosis High pitch @stenosis
Pulsatility (feel at body of access) Normal Increased (retrograde Decreased (antegrade to stenosis)
to stenosis)
Augmentation (pulsatility while Normal Normal Weak
occluding outflow)
Collapse with arm elevation Complete (forearm fistula) No collapse retrograde Complete antegrade to stenosis
(observe at body of access Partial (upper arm fistula) to stenosis
while elevating arm over (This does not apply to grafts.)
the heart level)
*Depends on flow volume of the access. Bruit becomes discontinuous with lower flow volumes.
Figure 3. Ultrasound images of a forearm radial-cephalic access showing a transverse and longitudinal view of the body of
the access with depth and diameter determination. Access vein and depth are adequate for needle insertion.
them in graph form. While B- and C-Mode ultrasound are ultrasound machines. Special attention is needed to make
relatively easy to learn, several additional steps need to be sure that the software is present and the settings are
mastered to correctly apply spectral Doppler. correct.
Spectral Doppler is required for flow volume determina- To obtain accurate flow volume measurements in a
tion. Timed average mean flow velocities (TAMVs) in a vessel, several rules need to be strictly applied (Table 5).
vessel as well as the diameter of the vessel for calculation With the understanding that only a straight vessel with
of the area are the parameters needed for the flow volume nonturbulent flow allows for correct flow measurement, it
calculation. TAMV needs to be obtained over several pulse is best to measure access flow volumes in the brachial artery close
cycles. The area of the vessel is calculated after measuring to the elbow or in a singular outflow vein as opposed to the “stick
the diameter of the vessel. zone”.10,12,18,19 Too often, the body of the access is tortuous
The following formula is used to measure flow volumes. and has varying diameters, and the flow is turbulent,
Flow volume in ml/min ¼ Mean velocity (TAMV) (cm/sec) resulting in inaccurate measurements. Also, it is of utmost
3 Area (cm2) 3 60 (sec) This formula is preprogramed in importance to measure flow velocities at the proper angle
Figure 4. Right forearm radial-cephalic autogenous access with poor augmentation. Ultrasound images demonstrate several
side-branches, which were then marked on the skin for planning of side-branch ligation as well as a fascia overlying the
vessel, which may require fasciotomy. At the level of the fascia, the depth is marginal for needle insertion and may need
additional tissue-removal (lipectomy) to make the vessel more accessible.
Figure 5. Left upper arm brachial-cephalic autogenous access, which suffered infiltrations. The B-Mode ultrasound
demonstrates that the vein is now at considerable depth, and a hematoma is overlying the vein. The diameter of the access
vein is sufficient. A map on the arm after evaluation of the depth can help with needle insertion.
(,60o), which is often impossible in a superficial vessel, of more than 25% over 4 months points toward access
such as the body of the access or the radial artery. Only a dysfunction.10
few studies compare the accuracy of ultrasound flow Sometimes, DVA can have too much flow, ie, greater than
measurement with both the brachial artery and outflow 1.5 or 2 L/min. High flow puts the access at risk of bleeding,
vein with that of invasive flow measurement.20 Figure 7 and high pressures, vein dilation with aneurysm
demonstrates the correct location for measuring flow formation. Patients with high flow are at risk to develop
volume in the brachial artery in a patient with a left steal symptoms and/or heart failure. With diagnosis of
forearm radial-cephalic autogenous access. Figure 8 high flow, it may be best to refer the patient for expert
demonstrates the images and measurements obtained for evaluation and possible treatment with flow reduction,
flow volume determination. ie, precision banding.23
DVA flow volumes can be highly variable. Flow volumes
can range from 200 to .3000 mL/min. The critical flow What Measurements Should Not Be Attempted with
volume of an autogenous access has been determined to POCUS
be around 400 mL/min. In a prosthetic access, the critical Spectral Doppler utilization for measurement of flow
flow volume is approximately 600 mL/min.21,22 A fistula velocities and determination of stenosis of the DVA is
with a flow volume below 400 mL/min will not sustain difficult because of the aforementioned reasons, and the
efficient dialysis at pump speeds of 350-400 mL/min, and values are difficult to interpret. They also have limited
a graft with a flow volume below 600 mL/min is at risk clinical use. Therefore, measurement of flow velocities
for thrombosis. In the event where serial flow volume along the entire circuit is not recommended to be a
measurements are performed, a decrease in flow volume standard for nonexpert vascular sonographers.
Table 4. Ultrasonographic Appearance of Commonly Seen Pathologies Associated With the Dialysis Access
Pathology Sonographic Appearance of Access-Related Pathologies
Seroma An-echoic round structure
Hematoma Hypoechoic/echo inhomogenous structure around the access vein
Abscess Hypoechoic/echo inhomogenous structure similar to a hematoma but with additional clinical signs of infection
Aneurysm Dilated segment of the access vein
Pseudoaneurysm Out pouching of the vessel with a pulsatile flow that appears on color and spectral Doppler
Tissue edema Cobblestoning pattern
Figure 6. Left upper arm brachial-cephalic access with “lump” and difficulties in needle insertion. B-Mode demonstrates
partially occluding thrombus with patent vessel underneath. The addition of color Doppler demonstrates continuous flow
into this partially thrombosed pseudoaneurysm.
Figure 7. (A) Left forearm radial cephalic autogenous access, which is superficial and tortuous. (B) and (C) demonstrate the
difficulty to place the probe on the access. There is no area on the access vein allowing correct positioning. (D) The probe is
now placed at the elbow, evaluating the brachial artery. In this position, the vessel can be insonnated at an angle ,60 , and
compression of the vessel is less likely.
for pseudoaneurysms, hematoma, and extravasation with are considered to put patients at risk for steal or congestive
B- and C-Modes. heart failure.
Heart Failure or Steal Symptoms. Access flow volume SUMMARY AND CONCLUSIONS
measurement can help diagnose a high-flow access. Even POCUS represents a very valuable extension of the DVA
though there is no standard definition of high flow, physical examination. It is portable, noninvasive, easily
generally flow volumes greater than 1500-2000 mL/min repeatable, and can be performed efficiently at bedside
Figure 8. (A) B-Mode image of the brachial artery demonstrating the direction of ultrasound waves as compared to the artery
and (B) the spectral Doppler for flow volume determination using the diameter and timed average mean velocities (TAMV)
over several heart cycles.
by trained nephrology staff. Although the training may 10. Vascular Access 2006 Work Group. Clinical practice guidelines for
take several weeks, the trainee is more than compensated vascular access. Am J Kidney Dis. 2006;48(Suppl 1):S176-S247.
by the immediate availability of pertinent information https://doi.org/10.1053/j.ajkd.2006.04.029.
11. Robbin ML, Chamberlain NE, Lockhart ME, et al. Hemodialysis
describing the DVA and the resulting diagnosis based on
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B- and C-Mode ultrasound in conjunction with the skills 12. Robbin ML, Greene T, Allon M, et al. Prediction of arteriovenous fis-
for performing a detailed physical examination should tula clinical maturation from Postoperative ultrasound measure-
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ACKNOWLEDGMENTS for vascular access assessment and cannulation in hemodialysis: a
The authors thank David Binder for his careful review and scoping review. Semin Dial. 2020;33(5):355-368.
editing of the manuscript. 16. Niyyar VD. Ultrasound-based simulation for cannulation in outpa-
tient hemodialysis units: an educational protocol. J Vasc Access.
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