PAGE NO. :.

DATE 1

SPECTROfLOURINETRy
masuemerrt e ucesenco tensty ata
T
narticdax A ummiacerro
radiatian usCslechiely kla
emrtrg 2es
uiscenc phesphejluaescence
ght _i3 mcidlent em
ubence - uchen a beom
theu emt viaiye Lbht aoddtieo
eexton sub adiaiem uà ncident
phsaphca12cence- Lohen_Jught 1ght
Cn emtConfinueus euen}
3ub.they
en certan
attes rcdentught 3 cut ol

Parthcable-

hecy uoxeYCenCee
Lohem a meleculo ue_m pomd sate then

Thi_state eld. aimalt stnte & when thig

Condittto n gioLrd stete old 2glt
oLmd stafe.
 PAGE NO.
DATE: I

oherm EMR gun tten aoms exciteg B

Cemclthem Heie alse ma onois &touend
is
tRen c Smglet excted state lonsabe

Hor achieomgsabilit 3 poce33 OCCLLY

OLohen molecile moLe om acund to exuted
Ztate ox urce -ea m blLo " there Collál
tHn c Collagen deactiateo
ohen_malecule m excuted sterte & lome
etaleuneK emeaq leelCvo)tken t me
te gacumd stote ahich raliackien A_
sntHed eldRucxescence.
be molecuule maue pom Smglet exctted to
tanplet exccted Starte The vbratien
elaxotien ccau 8 tmaues te gacund atate
ej phesphetiescemce
nd àte 's cut then al&e fer erne
ume_diafen.
- vibrctienal envgt
exccies.9ttte
Irrtesystem.

ADH
excutaliem acvegenc
phephooegemCA

Sgttt

tdte 1
 PAGE NO. :

DATE: I

SRECERePLAURMEIRy
Itexyetem rcemg Famasiiom bluo 2 2oencagetie-
vibcckiemal leuel-autg ditspm muuHiplicties-
LcOcence-
|SngRtexceted emuseien o 3imget qsoLme gtote
tcte Gadietien
phogphmeLcence
e
Faplet excited
Btocte.
ShngRt ztate- Au a m mlecuo oxe pomed ( )
Dothle 2tate- Am umbat 1g (1or Sigle 3pim
iplet tate- umbaked e e_9ame spo (11 r LL)_
e exctec state- ave npauhed but e
abn. ) opp.
tate
factox attecttg lourescence
Ccmugatien- A moleculo mugt _hove yn2crksortHem_
2ethot uv/uia adahm Caom be ahscrbed
thexe s ne abaarptlern aodudtien hem r e
Lol met be Rouieacence

Nakue e 3ubstituent grsuþpe

EDGikë Amimo-NH,),Hydtaoryl OH) qsEp
ephonce eLae9cerco.Jnteñaty
,0
EOG_LKe Nie-No), Caxböxyle (-CooHgab
educe uoeacence T

Rugut
Rigad stauchoe
Mese eALxeseencoetensite
l e sts- Legg M
 PAGE NO.:
DATE: I

Aousen
Bphen
Rigid tr. laxible sty.
eHect tempctcge-
neaso9e m tempottse leada te n
Call13tenn mcleclos
heselexe
eLsescert ntensitt, em ather bamo, m
Hemplead te m Cellisie ep molecula
Lohich eautt im leuregrent mten81ity

Collisem ae geduco, & lourescert

actibity-
n_gthcs and i n lesg eLeDCYo
Collisem t ad rcduco einescect aetiuit
Oxygen- 0. uoegcent I m 2 OCu
wo
oxid iee ub
memlaaescent Su
2 T Bxenches ()luexELerce bcen
Ma
poosamagnetic pacpextiesemcleCular eoerq-
Q tfas tiplet gscimdState
ettect o pH deperd on chemical stz o
moclecilo.
eq-Anilme m neutxalalkalime meduu
ges isible -ueaence but m
Hem quue acdi
VCgion only. *
 PAGE NO.
DATE: /

umdL`gocuhted
& de
hm acidudc Ceno are Condtiem
fhenol alkalme
but m
euacecerco
mat gaie geedoLaeeent
aae_diaeecihtecd g u e
they
AmL n g
ntenstu f mcuilent liaht- T
loLrescence T alse
m He sample dedombsaitem-
iNvis
abeexptiem.
ac"
Ihotnchemical phatoeachem de.
Semetmes leads
de
2ezm-
CasesReu. Cannat be
I n such is mat Stemgli
L a e l m g t h which
Hence, a eheBen te aued }uch
ueh

Labsabed sheuld he 20/ E þc89ible

ee ubte
elatiem cttevobe

edect e loncentiatien=
I eone. o &ub.
Aounescence
the
only oben absexbarco i2 lesg tham 0-02
euescenco TL betex_
T xLa
etieenc

kouvegcerce

B ti s ia tue m l&O Cnc [ugmi) but t

bilgh Conc. ( g m i ) _ t d o e s no obey
necity.
'neay

Co ne
 PAGENO.:
DATE: I

elbraotton eu
enench CHgh Cene.
7uneo
one

AveNCHERS
e on oceggiatt
Guenchng- xefexs
educesRe Qeuaescent Tea giien au
may OcoG duete: va1ous yactex lh
lhig
pH,Conc., temp, , a 2, heau metaltt
g Guenchin9 agenta2-
uecbm agnt Tybleo flouaobbae
Thieeymate Anthsac ene
ehloicte Gunme
Todne uptapham
Dis fide lsasihme
NHnc Oxide Napbtialene
Lollsena? Auenchmg- accurs by isteractiem
a Quemchex sleculg CA) Xrt8aa
ecited molecule. tBe leescemt aub (t
AsmpliPled mech Canbe uomtten
fe cdesCabe tRi3 prece,2

P+
Hese, the texactiem re9ults_m the-
dasaibatiom
men- eachve
e exctcchien emerqs
y by a
enerqy ransyer rom
 PAGENO.:
DATE:

de A out ex lea ueICence

Conc.
Sel Bugmchng- At lon
lmeanty ohserueo
CmgImR)s a m e sub, T m the
high high Conc.
OMESCernt I obaexoed.
Aueochirg-
hi phenomena elc set4
HOghCene Cuugmt)
uoNexene
touo Conc.(ug
saytcone
7uneotnty uenctrkaF
ICLng-
Cone
ehemica Auemchmg-
O
Dueto Ncmeus tadtx like chamge m pH, ho
reate
halides 8 EG S heau metol áaxe
chemital Suench -
CollagenBuenchog esutt ofbeLEOL Yacfexg
like mes3Le Ralucte,beniat metal temb
Collagem_0re henco
N Y wbexe ma
Auencheng take place
lmstaumenfahiem
Bamle
tignt Bocste
Jholde onc Fexrteko
emisse
exctahen emu'ssibn amble holde_
t e fMrono Mono
Detecto3
Deteotey Readout daulto
Readnut

looce
 PAGENO.:.
DATE: I

a ligRA
manly uSed
SoLace xecn_c
Xenonpaoduuces Cootinuou 2pectum

Nie
NonachnomacoX
emiSSon
e x c t a h n

m2e% Tacdibtiem
Allons only adiaHen
moleculos enluemtted by am
ohich excites molecule3 e dotectey
arfom3
Samble holder- te held the ample
glas 5
ade up Auty e ghabe
Could besqsuave lcuidnical n
Detecto.3 ill Conuert ligkt erergE
Sigmalthat me duzpla,yed
mte eloctzica
e n omdeut doiteg.
used as dotecter
PMT à Commcoly
RuEOscemespec.

autbLut unentsom
Readeut dauico ke
brte mea sumng edlesikó hat
detectex ia fed
mditateg bxtent e t o n uascence
ApplicotHiemg-
useJm-
OluTescet ndicaterrg maumly ColmE
e - +lusescem
ci baSe Lt-hcn
grem-
Qunne sulphate 2 blue-uidlet
Acacdine Canesn - Uiolet

PhaxmacOutica nalai&
 PAGE NO. :
DATE:

Comb Reagett exctatilom A

Hudnocotison 75/v/HSoy
m ettamel
Y6o

Nidottnamide eyanogen chlovide 250

Detesmmatien e Vit B, 8 B2
Lagud ehnomatoqraphy flucrescene ug an imp.
methedef determmmop lompcurds ashep-
appeox at he end ebmemategrathyu oxe
Capilaay_elkctiepbaesug eelumm.
5Cigmic analyei3 ualtahiue 8tantitatiue_analyacs
eeparnic aomatie Comp m_air þa00tants,
auteobile exhaute,etc.