"Evaluating the Therapeutic Potential of Abrus precatorius (Saga),

Clitoria ternatea (Pukingan), and Oldenlandia diffusa (Ulasiman-kalat)

Leaves Extracts for Managing Open Wounds; Utilizing Lactobacillus

reuteri"

Rationale

This study aims to increase our understanding of three plant extracts to manage open

wounds without utilizing human participants. The use of herbal remedies is intended to improve

health and facilitate healing without the use of chemicals or medications. As a result, it is less

expensive, has fewer negative side effects, and offers greater health benefits. Wounds, whether

caused by accidents, medical operations, or injuries, are common and frequently necessitate

skilled management to prevent infections and promote speedy and uneventful recovery.

Traditional herbal treatments have a long history of use throughout cultures and have been

recognized for their potential medicinal capabilities, including wound healing. This study

focuses on three different plants: Saga (Abrus precatorius), Pukingan (Clitoria ternatea), and

Ulasiman-kalat (Oldenlandia diffusa).

The purpose of this study is to determine the efficacy of the extracts of the leaves

Saga (Abrus precatorius), Pukingan (Clitoria ternatea), and Ulasiman-kalat (Oldenlandia diffusa)

in activating Lactobacillus reuteri in order to examine their ability to heal a wound, by

stimulating a probiotics. One probiotic that is present in many parts of the human body,
including the skin, is Lactobacillus reuteri (Mu, et al., 2018). The results of animal research by

Erdman and Poutahidis (2016) have shown that, Lb. Reuteri has the ability to speed up the

healing of skin wounds. This will determine the similarities and differences between a

commercial wound treatment and Saga (Abrus precatorius), Pukingan (Clitoria ternatea), and

Ulasiman-kalat (Oldenlandia diffusa) in terms of their reactions to Lactobacillus reuteri. This

will be a crucial chance to expand our understanding of this plant's ability to cure wounds

without using human participants. This study aims to provide knowledge and information on

how these plants might be used to treat wounds instead of conventional medicine, as a less

expensive, environmentally friendly, and non-toxic alternative.

Research Question

1. What is the significant effect of Saga, Pukingan, and Ulasiman - kalat in treating open

wounds.

2. What is the effectiveness of Saga, Pukingan and Ulasiman - kalat in treating open wounds.

Expected Outcomes

The researchers expected outcome of the study is to prove that plants Abrus

precatorius (Saga), Clitoria ternatea (Pukingan), and Oldenlandia diffusa (Ulasiman-kalat) will
be able to treat open wounds. The researchers expected that the experiment would be safe and

effective. The researchers also look forward to see changes in the lactobacillus reuteri in the petri

dishes to determine which is the most effective. They also expected that they did the correct

preparation and exact dosage. They also expected that their data analysis technique would be

efficient.

Procedures

In this study, experimentation will be done to conduct the study to prove that the

following plants Abrus precatorius (Saga), Clitoria ternatea (Pukingan), and Oldenlandia diffusa

(Ulasiman-kalat) can be used to treat open wounds. A total of 10 petri dishes are going to be

used, wherein treatment groups will be using 2 plates each. Duplication is done for each group in

order to ensure an accurate and reliable results.

Abrus precatorius (Saga), Clitoria ternatea (Pukingan), and Oldenlandia diffusa

(Ulasiman-kalat) will be collected in the necessary quantity by researchers. The leaves will be

mashed with a mortar and pestle. After crushing the leaves, they will be placed in a cheesecloth

to extract their juices. After squeezing, the extracts will be placed in a container. The extracts

will be placed in individual containers, with the final container containing a blend of the three

plants extracts. Each agar plate shall be coated with Lactobacillus reutri. The samples will be

distributed equally throughout the surface of the solidified Agar. All petri dishes will be placed

in sealed plastic bags to avoid spreading of bacteria.

Risk and Safety

This study contains microorganisms specifically lactobacillus reuteri. Lactobacillus

reuteri is used to test the mixture of Abrus precatorius (Saga), Clitoria ternatea (Pukingan), and

Oldenlandia diffusa (Ulasiman-kalat) since human participants cannot be used because it will

higher the potential risks. The risk and safety assessment form will be provided by the

researchers for the safety of the researchers and other persons that will be helping them.

Chemical and biological hazards might be encountered inside a laboratory. The following forms

must be filled in by the researchers before proceeding to experiment: Checklist for Adult

Sponsor (1), Student Checklist (1A), Research Plan/Project Summary Instructions, Approval

Form (1B), Qualified Scientist Form (2), and Risk Assessment Form (3).

Data Analysis

The researchers will rate the efficiency of the leaves extracts on a scale of 1 to 3. The

term “efficiency” refers to the extract's inability to grow bacteria in the petri dish. The term

“effective” refers to the extract's ability to grow some bacteria in the petri dish. Finally, "most

effective" signifies the extract's ability to grow many bacteria in the petri dish.
Bibliography

Erdman, S. E., & Poutahidis, T. (2016, January 1).

Microbes and Oxytocin. International Review of Neurobiology.

https://doi.org/10.1016/bs.irn.2016.07.004

Mu, Q., Tavella, V. J., & Luo, X. (2018, April 19).

Role of Lactobacillus reuteri in Human Health and Diseases. Frontiers in Microbiology.

https://doi.org/10.3389/fmicb.2018.00757
 Lopez National Comprehensive High School

Maharlika Highway Lopez, Quezon

"Evaluating the Therapeutic Potential of Abrus precatorius (Saga),

Clitoria ternatea (Pukingan), and Oldenlandia diffusa (Ulasiman-kalat)

Leaves Extracts for Managing Open Wounds; Utilizing Lactobacillus

reuteri"

Group 11

Life Science

Proponents:

De Castro, Yurrica Arwa D.

Mariquina, Chandra Jules R.

Tadeo, Ava Nicole L.