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UNIT - 5 - Hydrophobic Interaction Chromatography
UNIT - 5 - Hydrophobic Interaction Chromatography
It was described for the first time by Shepard & Tiselius in 1949 using the term
“salting-out chromatography”. Finally, Hjerten in 1973 described this
technique as the “hydrophobic interaction chromatography” based on the
retention of proteins on weakly hydrophobic matrices in presence of salt.