Student Name:

LAB #2
LAB SKILL: ORR
TITLE: Separation Techniques
TOPIC: Chromatography
AIM: To separate the solute components in a black ink pen or a mixture with screened methyl by
paper chromatography
APPARATUS/MATERIALS: 100ml beaker, chromatography paper, water, ethanol, black pen,
petri dish
METHOD:
1.Draw a horizontal line 10mm from the bottom of the chromatography paper
2.Mark an ‘X’ in the centre of the line
3.Use the black ink pen to make a spot on the ‘X’
4.Pour an equal amount of ethanol and water into the beaker
5.Carefully insert the chromatography paper into the beaker with ethanol and water. (about
5 mm height)
6. Make observations and calculate the Rf values for the different components of the ink
OBSERVATIONS:
DIAGRAMS:
 Before - (draw a copy of your spotted chromatogram)
 After – (paste your dried chromatogram beside your before drawing)
 Describe what is observed at the beginning, during and at the end of the lab
 Calculations of Rf values
DISCUSSION:
 Define chromatography
 Identify the mobile and stationary phases in this lab
 Explain what the Rf values tell you about each component
CONCLUSION:
 Your conclusion must be answer to the aim
Student Name:

LAB #2
LAB SKILL: ORR
TITLE: Separation Techniques
TOPIC: Chromatography
Aim: To separate the solute components in a black ink pen by paper chromatography
Specific Objective: Section A, Specific Objectives 2.2
Skills Assessed: Observation, Recording and Reporting

Number CRITERIA Marks

s
1 Observation 3
A Colours listed that the ink separated into 1
Stated order of colours as they appeared on chromatography paper 2
2 Recording 3
Diagram
A Labelling 1
B Title 1
C Neatness 1
3 Reporting 4
A Grammar 2
B Method 2
C
D

TOTAL
10
Student Name:

Lab 2: Chromatography [STUDENT VERSION]

Skill: Analysis and Interpretation

Characteristics Marks

A. Definition of Chromatography 1

B. Identify the mobile and stationary phases in this lab 1

1

C. Component that moves the furthest 1

D. Component that moves the slowest 1

E. Reason substance move the furthest or slowest 1

F. Calculation of Rf value (1 Mark for each component) 2

G. Conclusion related to the aim 1

H. Source of Error/Limitation/Precaution 1

TOTAL 10