Page 1 of 2

2907069

Certificate of Analysis
Vessel / Object: 5 Job No: 158-24-00040
Location: Harlingen, TX / Key Petrol Terminal (United States) Date Sampled: 01/12/24
Job Type: Discharge Date Tested: 01/13/24
Product Grade: Regular Gasoline Version: 1 / 13 Jan 2024 01:53
Client Reference: Biosilver LLC / 4670085

Sample Sample ID, Type & Description

158-24-00040-022 ST 5 Composite After Discharge

Method Test Min Max Result Units Remarks

ASTM D5191 DVPE @ 100F° (ASTM EQ) 11.5 14.45 psi
ASTM D5191 Volume of Container in mL 1000
ASTM D5188 Vapor-Liquid Ratio (TV/L)=20 47 40.3 °C
ASTM D86 Distillation Evaporated
Procedure Used Automatic
Barometric Pressure 101.2 kPa
IBP 20.0 °C
10% 60 35.8 °C
50% 77 116 77.7 °C
90% 185 140.0 °C
FBP 225 183.5 °C
Residue 2 1.0 %vol
ASTM D4052 Gravity by Digital Density Meter
API Gravity @60°F 66.9 °
Specific Gravity @ 20/4° C 0.7084
ASTM D3227 Mercaptan Sulfur 20 3 mg/kg
ASTM D130 Copper Strip Corrosion Rating @50°C/122°F for 3 1 1A
Hours
ASTM D381 Gum Unwashed and Solvent Washed
Unwashed Gum Content < 0.5 mg/100ml
Solvent Washed Gum Content < 0.5 mg/100ml
ASTM D525 Oxidation Stability @100°C 240 > 240 mins
ASTM D2699 Research Octane Number
Research Octane Number by Compression 87.2
Ratio
Room Barometric Pressure 30.0 in. Hg
Intake Air Temperature 99 °F
ASTM D2700 Motor Octane Number
Motor Octane Number by Compression Ra- 82 82.8
tio
Room Barometric Pressure 30.0 in. Hg
Mixture Temperature 286 °F
ASTM D4814 Antiknock Index (R+M)/2 87 85.2
ASTM D1319 Hydrocarbon types by FIA (Aromatics, Olefins &
Saturates)
Dyed Gel Lot Number 3000000984
Aromatics 14.8 %vol
Olefins 7.3 %vol
ASTM D3606 (Proce- Benzene 2.0 0.62 %vol
dure B)
ASTM D2622-16 Total Sulfur Content 80 16.8 ppmw

AmSpec LLC - 17000 R.L. Ostos Rd - Brownsville - TX - 78521

This document is issued by the Company subject to the Terms and Conditions at https://www.amspecgroup.com/terms-conditions. Any holder of this document is advised that informa-
tion contained herein reflects the Company’s findings at the time and place of its intervention only and within the scope of the Client’s instructions. The Company’s sole responsibility is
to its Client and the Company disclaims any liability to third parties. Any alteration, forgery or falsification of the content or appearance of this document is unlawful.
 Page 2 of 2
2907069

Certificate of Analysis
Vessel / Object: 5 Job No: 158-24-00040
Location: Harlingen, TX / Key Petrol Terminal (United States) Date Sampled: 01/12/24
Job Type: Discharge Date Tested: 01/13/24
Product Grade: Regular Gasoline Version: 1 / 13 Jan 2024 01:53
Client Reference: Biosilver LLC / 4670085

Sample Sample ID, Type & Description

158-24-00040-022 ST 5 Composite After Discharge

Method Test Min Max Result Units Remarks

ASTM D4815 Oxygenates in Gasoline
MTBE < 0.20 %wt
Methanol < 0.20 %wt
Ethanol < 0.20 %wt
Isopropanol < 0.20 %wt
tert-Butanol < 0.20 %wt
n-Propanol < 0.20 %wt
sec-Butanol < 0.20 %wt
DIPE < 0.20 %wt
Isobutanol < 0.20 %wt
n-Butanol < 0.20 %wt
TAME < 0.20 %wt
ETBE < 0.20 %wt
Total Oxygen Content 2.7 < 0.01 %wt

__________________
Ezequiel Castillo
Laboratory Technician

Ezequiel Castillo
__________________
Ezequiel Castillo
Laboratory Technician

AmSpec LLC - 17000 R.L. Ostos Rd - Brownsville - TX - 78521

This document is issued by the Company subject to the Terms and Conditions at https://www.amspecgroup.com/terms-conditions. Any holder of this document is advised that informa-
tion contained herein reflects the Company’s findings at the time and place of its intervention only and within the scope of the Client’s instructions. The Company’s sole responsibility is
to its Client and the Company disclaims any liability to third parties. Any alteration, forgery or falsification of the content or appearance of this document is unlawful.