3/28/23, 6:29 PM Rapid detection of the main carbapenemases in Brazil directly from spiked blood culture using the

spiked blood culture using the RESIST-3 O.K.N. immunoassay - PubMed

Rapid detection of the main carbapenemases in

Brazil directly from spiked blood culture using
the RESIST-3 O.K.N. immunoassay
1 2 1 3 1 3 4 1 2 5 6 7
Priscila Lamb Wink , Amanda Silva Martins , Everton Inamine , Tanise Vendruscolo Dalmolin , Afonso Luís Barth

Affiliations
PMID: 31270693 PMCID: PMC6863339 DOI: 10.1007/s42770-019-00109-y
Free PMC article

Abstract
The emergence of carbapenem-resistant Enterobacterales (CRE) is a matter of public health concern. Carbapenemases are the main mechanism of
resistance among CRE, and its rapid detection is essential. The detection of carbapenemases usually requires culture-based methods and molecular
assays, which may be costly and need long turnaround times. Recently, an easy and rapid immunochromatographic assay for carbapenemases (OXA-
48, KPC, and NDM) detection based in lateral flow immunoassay with specific monoclonal antibodies on a nitrocellulose membrane has been
developed. We aimed to evaluate the RESIST-3 O.K.N. in colonies from pure culture as well as in spiked blood cultures with Enterobacterales. All
carbapenemase producers (CP) presenting the OXA-48-like, KPC, and NDM enzymes presented positive results in both pure colonies and spiked blood
cultures. None of the carbapenemase non-producers (CNP) presented positive results in the tests. A total of 97% CP isolates presented positive results
in pure colonies in less than 5 min. For CP directly from blood culture, the mean time to positivity for OXA-48-like and KPC was 1 min, whereas it was
25 min for NDM. Our results indicate that this immunoassay can be used to detect carbapenemases directly from blood culture bottles in a routine
diagnostic laboratory, which would reduce the turnaround time of CP detection.

Keywords: Carbapenemase; Multiplex immunochromatographic assay; RESIST-3 O.K.N.; Rapid diagnostic.

https://pubmed.ncbi.nlm.nih.gov/31270693/ 1/2
3/28/23, 6:29 PM Rapid detection of the main carbapenemases in Brazil directly from spiked blood culture using the RESIST-3 O.K.N. immunoassay - PubMed

Figures

Fig. 1 Evaluation of the

tests results…

https://pubmed.ncbi.nlm.nih.gov/31270693/ 2/2