Serwoja

RE- Rheumaoid fackor

late ogan inabon
Fest
Reverse
Pintple Padsive ogaminakon test

Pro Cedure: Posikve

Pa bent's Sernm,
Add one dop of
Control Seva
on he espechve
and Ne9ahve

20neS of a test plate

Rheumatoid rlagent
(atx
Add one drop o4f

Mix enty
mthun mimmtes.

ina hon
Look r 8tm

ReCult
Posi bve Aaghninahon

eabve No A0nnination

Iotepre taion
Somple TU{m
Undimked
16 TO
1 Dim kon

3 i mkon
 Sero logm

ASoL Anti . Streptolysin oj - latez ogynknabkon

test

Passive agotminabon test

Piniple :
Prote dure
of Pakents Senum,
Add one drop
Sevw on he
Conhol
PosiBve and Megaive
Cpecve 2 cnes of a t e s t pate

ASO reagent
Add one drop af (ate

Mix 9ent

inutes.

na ion wih in
Look for amuh

ReSnlt
Aggmbna kon
Posikve
Negnbvve No 33ubinaion.

terpe tahon
eo Ivm
Undi mted Sample
400 1ml
1 Dilubion
n Diuion
l600 Tu
3 Dimbon
 Seeona
CRP IC Reathve Proten latex agaubna hon
tes
Reverse

Priniple Passive agalwinabon test

ProCe dnuYe
Add one drop of Pabents Seum, PoSibve

Cvntwl Sera On the respecive

and Neaabve
2oneS of a test plate

Add One drop of loatex (rP rtagenE

Mix ent

9minahon wihin amnutes

Leok r

ReSnlt
PoSikve Aggminabon
v No aghmbna hon.
Negai
Interpretaion :

Undimted Somple- mglLit

1s Dhbon a m ib

rd piLukon 4
4 mgLi
 9erolog
Widal Fest

Tube test
Principle a09tmkinahon
Proednure
Pakents Semm is Senally daluted In nomal

test hubes,
salune in Felix bhebe for o anauination,
preyer's tube for H aggutinaion
. To eath Set of aimted sea, reSpe ckve four

anbigen Snspensioni ( To, TH, AH,BH) ave added .

Conbo wbes Contaiing he ankgers and homal

Saline $hould ke kept check fr ausegjhninahon

.Test ku bes a?e inmbated waterbath at
tn
3ic
bvermghb
Results
Negahve Buton omaion oC Curs

PoSihre:- 0 ag3mbinahon Compalt 9ranu lar

Chal ky chumps with clem Super nakant
fid .

H ggminabon -arge
loose f bnfby
with (Car
Cotlon -

Wcoly cwmps
supermoknnt flnid.
Sibcant hter :
o eal t
More than
O ajminaion
loo d bhtbon.

Moye Fhan O enal

Haggminahon
2oo dimhon.
 Se roboa

RPR Ropid Plsma Reagin.

Printiple Side Flo cm labon test

(edare

Add One drp of pabent's Seum, Posikve

and negnhr Con bol Se in the

espethve 2one 04 RPR Cavd

Add one d p 0f RPR- anhgen

Mix genty

Plae he RPR Card in he Rotatr

Rotate min at loo k pM.

Kead the resnlt

Resnht
Presente of Foctnla hion
Reative
Abente of Floctulahion.
Non Reatkve -

I o t e p r e kabon :

Vndimked Sample :2 Dim hon.

T°FDi mbon
Dimhon
Dimhon.
2 74 Di mbon
1 6 Dimbon
3 Dimhon
3 2 Diuion .
Di m ion
 Se
ELTSA E2yme Linked Jmmuno Sorbeot Assag
Prnip le:- Anbgen or An hbody is adsobed

On h e Sohd phase. TisF S ennmis add ed and

innbated for he formaien f ambgen- an Hboo

Compli, Unboun d Componen are remored

Wsn The amount 2f bcund frn ch'n iS

measued by addibon nked

enyme
an h- human Snbs hrate
tobndin. and apYvprare

obour and he
prdn Ce Prodmchs dekeched y
ELTSA reader

Cedre
in ath
IAdd appoprate
ume of _dibment

well0f
Milo ire plate
EsF Senm
Add
apppriate vo ume vf
in resseckve wels
Posibve and vegakre Conho
adhsive lm and
wih
Cove the plate
3 r

ime.
innbare
a t 37c far Spe Ci h cd
rKh
the velk by
and
Contenks
he
4Aspiz1te

wah Sombon.

Conjnge
JnCubate aE 37 c for
5 Add

Spcihd kme
he contenk. & wash he wel
Aspime
Add bufered Sabs hate ard Chomaaty *
Spe i Bred
Somiors. Incubare at 27 e

ime
lad absorbone
7 Add Skop So mbon ahd

a 450 nm f bbo nm Yeatr

ya mlwpAre

oD Lophtal Densihy] -mesured

Resuls
less han
Negabve 14 0D vaue of Sample is

of Control by ien tntvr.

OD Negabve
1f 09 vame of Sample tesFed
Posihve
ex ceeds OD of Negakve Conrod
iven facor.