MASS SPECTOMETRY

1. The liquid from _________________ is introduced directly into the ionization

source.
2. _____________ is the most common ionization source for liquid chromatography
and mass spectrometry
3. _____________ is applied to a corona discharge needle, which emits a cloud of
electrons to ionize compounds after they are converted into a gas phase.

_____________ 4. A soft ionization technique that is typically used to determine the

molecular weights of proteins, peptides, and other biological macromolecules
______________ 5. The source contains a heated vaporizer to allow rapid desolvation
of the drops
______________ 6. Used to analyze biomolecules such as peptides and proteins.
Converts samples to gaseous ions and applies an electrostatic potential
_______________ 7. A kind of mass analyzer that applies an electrostatic potential to
accelerate the ions through an elongated chamber called the flight tube.
_______________8. Commonly used method to determine a protein's mass, protein
samples are mixed with an appropriate amount of matrix solvent and placed onto a
stainless-steel plate.
_______________ 9. Tandem mass spectrometry is also named as?
_______________ 10. Most commonly used is the ‘product ion scan’ which involves
isolating the ion of interest
_______________ 11. Is a technique that is used across a varety of fields due to its
accuracy and ability to discern its prescence of multiple chemical spicies, even at very
low concentrarions.
_______________ 12. Is defined as the mass of a given compound divided by the width
of the corresponding peak and is commonly designated by the term full width at half
maximum (fwhm)
_______________ 13. The most common means of detecting ions employs an electron
multiplier. In this detector, a series of dynodes with increasing potentials are linked
_______________ 14. Was brought into the operating room and the analysis was
conducted during the surgery itself. This work is made possible through the use of
desorption ambient ionization, a method that introduces samples directly into mass
spectrometry without extraction
_______________ 15. Is used to amplify dna from the unknown pathogen and esi-tof
ms is used to calculate exact masses of these pcr-generated dna fragments.

16. The most common form of ionization used in gas chromatography/mass

spectrometer is _______________.
17. Electrospray ionization involves passing the lc effluent through a capillary to
which a _____________ has been applied.

PROCESS OF ELECTROSPRAY IONIZATION

_________________18. Under the action of a high-voltage electric field, the sample
solution is ejected from the capillary to form a charged droplet.
_________________ 19. The size of the charged droplets reaches the nm level, and
the ions in the droplets turn into gas phase ions.
__________________ 20. The droplets entering the spray chamber are evaporated by
the countercurrent of the heated dry gas (like nitrogen gas), and the diameter of the
droplets becomes smaller, and the surface charge density increases

21. In tof – time to flight - the ionization of the low molecular weight matrix is
distinguished from the _________________ peptides and proteins when it
reaches the detector.
22. _________________ is the most common mass analyzer in use today
23. In quadrupole - the electric field on the two sets of diagonally opposed rods
allows only ______________________ value to pass through the analyzer to the
detector.

24. ___________________ can be thought of as a modified quadrupole.

25. ____________________- is a technique used to analyze a sample either by
using two or more mass spectrometers connected to each other (tandem-in-space)
or a single mass spectrometer by several analyzers arranged one after another
(tandem-in-time)
26. In a three-dimensional ion trap, the four rods, instead of being arranged
parallel to each other, form a three-dimensional sphere in which ions are
_____________.
27. A common form of ms/ms is to link ________________ in a series. This
instrument is referred to as a triple quad
28. _______________- mass spectrometry can be used for the identification of
pathogens in modern microbiology laboratories.
29. Putting isolated ____________________ onto the maldi plate and ionized,
which results in a protein fingerprint of the species. (with this they can compare it
with their digital database of the species.)
30. Mass spectrometry at the point of care use of portable mass spectrometry to
analyze _____________________ intraoperatively