Supporting information

Neutral mechanisms and niche differentiation in steady state insular

microbial communities revealed by single cell analysis

Zishu Liua, Nicolas Cichockia, Thomas Hübschmanna, Christine Süringa, Irina Dana Ofiţerub,
William T. Sloanc, Volker Grimmd and Susann Müllera

a
UFZ – Helmholtz Centre for Environmental Research, Department of Environmental Microbiology,
Permoserstr. 15, 04318 Leipzig, Germany.
b
School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU,
United Kingdom.
c
Department of Civil Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom. dUFZ –
Helmholtz Centre for Environmental Research, Department of Ecosystems Analysis, Permoserstr. 15,
04318 Leipzig, Germany.

Contents

S1: Continuous reactor set-up................................................................................................................. 1

S2: Set-up of the disturbance experiments ............................................................................................. 2
S3: Overview of standard parameters measurement in this study ........................................................ 2
S4: Procedures of cell samples preparation ............................................................................................ 4
S5: Cytometric analysis............................................................................................................................ 5
S6: Reproducibility of cytometric measurements ................................................................................... 8
S7: Data lists for biostatistical evaluation of cytometric data................................................................. 9
S8: Biostatistic evaluation using gate-based cytometric data and standard parameters ..................... 21
S9: Comparison of different methods in calculating diversity metrics with cytometric data............... 25
S10: Quantification of community stability properties after drifts or disturbances ............................. 30
S11: Fates of subcommunities .............................................................................................................. 32
S12: Sequencing protocols and details.................................................................................................. 35
S1: Continuous reactor set-up
Experiments were performed with cells originating from an activated sludge basin of a wastewater
treatment plant (Eilenburg, Saxonia, Germany, 51°27'39.4"N, 12°36'17.5"E) and stored at -20 °C. Cells
were precultivated in a 1 L shaking flask containing 300 mL of a mixture of peptone medium and
synthetic wastewater (v:v = 2%:98%) on a rotary shaker at 125 rpm at 30°C for 17 h. The medium
contained 0.198 g L-1 peptone (from meat), 0.2 g L-1 meat extract, 0.219 g L-1 yeast extract, 0.1 g L-1
glucose, 0.49 g L-1 Na-propionate (filtered), 0.0059 g L-1 CaCl2.2H2O, 0.0294 g L-1 KCl, 0.06 g L-1 NaCl,
0.04 g L-1 K2HPO4, 0.2156 g L-1 KH2PO4 and 0.0196 g L-1 MgSO4.7H2O, which were purchased from:
Merck KGaA (Darmstadt, Germany), SERVA Electrophoresis GmbH (Heidelberg, Germany) and Carl
Roth GmbH(Karlsruhe, Germany).
Bioreactor cultivations were routinely performed in five isolated 1 L bioreactors with a final working
volume of 800 mL medium. Each of the five bioreactors was inoculated with 50 mL of an identical
preculture. The environmental conditions were set to 30°C (Incubator Hood TH 25, Edmund Bühler
GmbH, Hechingen, Germany), 350 rpm using a multipoint magnetic stirrer (Thermo Electron LED
GmbH, Langenselbold, Germany) and stirrer bars (45 × 8 mm, Labsolute®, Th. Geyer GmbH,
Renningen, Germany), and to an aeration rate of 150 mL min-1 compressed steril filtered ambient air
by using a rotor gas flowmeter (six measuring channels, Analyt-MTC GmbH, Müllheim, Germany). The
reactors were constructed from 1 L glass bottles (Duran®, customized at glass workshop of UFZ,
Leipzig, Germany) and were equipped with several tightly closed ports for maintaining sterile reactor
conditions. The loading rate was set to 1.08 g COD L-1 d-1 (chemical oxygen demand), the dilution rate
to 0.72 d-1 and the hydraulic retention time to 33.3 h by a a microprocessor controlled dispensing
pump IPC-N 12 (Ismatec®, Cole-Parmer GmbH, Wertheim, Germany). The cultivation mode was
continuous which was stably established after five times volume exchange for all five reactors in
parallel. The five reactors were sampled with high frequency (65 times in 91 d) by harvesting 2 x 5 mL
using sterile syringes connected to the ports. Syringes were replaced and ports were sterilized with
70% Ethanol each time before and after sampling. The whole experiment was run for 91 days. A sixth
reactor was operated with the identical set up procedure but without inoculation of the
precultivated microbial community to verify sterile reactor practices. Reactor construction and
experimental setup is shown in the schematic graph (Fig. S1.1).
The phylogenetic affiliation of members of the inoculum and the reactor communities is listed in S12
(Table S12.2), which was determined by using Illumina® MiSeq sequencing. Community dynamics
were measured on a single cell level by flow cytometry and used to calculate ecological metrics (main
text and S4-S11). Abiotic parameters were measured according to German guidelines and listed
below (S3).

1
Figure S1.1: A: Set up of the reactors. B: Experimental setup: all five reactors were run in an identical mode.
Two of these were not disturbed and served as controls (C1 and C2), while the other three reactors were
treated with identical temperature changes (D1, D2, and D3).

S2: Set-up of the disturbance experiments

Two of the five chemostats were performed under unchanged conditions and regarded as control
reactors (C1 and C2). The other three chemostats (D1, D2, and D3) were exposed to identical
disturbance situations which was a repeated change in temperature from 30°C to 40°C. After
reaching the continuous reactor equilibrium (five volume exchanges), the first increase in
temperature was started at day 7 to 35 °C for three days (8 d - 10 d), and to 40°C for 18 days (11 d -
28 d), followed by a temperature drop to 35 °C for three days (29 d - 31 d) and to 30 °C for another
18 days (32 d - 49 d). The second disturbance was identically processed during the following 42 days
(50 d - 91 d).

S3: Overview of standard parameters measurement in this study

Abiotic and biotic parameters were measured routinely. In addition to the reactors in the control and
disturbed group, one reactor was set up as abiotic control (AC) without any biomass growth, and
operated under the same conditions.
The OD (optical density) was measured spectrophotometrically at 600 nm (Ultraspec 1100pro,
Amersham Biosciences, Buckinghamshire, United Kingdom) using a 5 mm cuvette (100-QS, Hellma
GmbH, Müllheim, Germany) in addition to pH (EL 20, Mettler Toledo, Switzerland), and EC (electrical
conductivity, PHT-027, Graigar Group, China). All other parameters were analyzed with
NANOCOLOR® test tubes (Macherey-Nagel GmbH, Düren, Germany) according to German DIN
guidelines: NH4 (ammonium of supernatant, DIN 38406-E5), PHO (phosphate of heated supernatant,

2
DIN EN ISO 6878-D11), CODs and CODt (chemical oxygen demand of supernatant [-s] and total
sample containing cells [-t], DIN ISO 15705:2002). Supernatants were obtained after centrifugation at
21,000 g (Heraeus Fresco 21 centrifuge, Thermo Scientific, Langenselbold, Germany), 10 min and 4 °C.
CODt minus CODs is the CODb (COD biomass, Bullock et al., 1996), which indicate the contribution of
the microbial biomass to CODt. Numbers of cells per sample were counted by flow cytometry (S5,
measurement of cell counts). All data are shown in Figure S3.1.

Figure S3.1: Analysis of abiotic and biotic standard parameters over a time period of 91 d. From left to right:
abiotic control (AC), undisturbed reactors (C1 and C2), and reactors disturbed by temperature changes (D1, D2
and D3). Measured parameters are: temperature (T), pH, electrical conductivity (EC), ammonium (NH4),
phosphate (PHO), chemical oxygen demand of supernatant (CODs), of the total sample (CODt), and COD
biomass (CODb); number of cells and optical density (OD).

Reference
Bullock, C. M., Bicho, P. A., Zhang, Y., and Saddler, J. N. (1996) A solid chemical oxygen demand (COD)
method for determining biomass in waste waters. Water Res 30: 1280-1284.

3
S4: Procedures of cell samples preparation
1. Preparation of cells for measurement of community composition
Step 1: Cell sample fixation
For flow cytometric analysis, 4 ml samples were taken from the bioreactors, centrifuged (3,200 g, 10
min, 4 °C) and the supernatant discarded. The cells were washed with phosphate buffered saline
once (PBS, 6 mM Na2HPO4, 1.8 mM NaH2PO4, 145 mM NaCl, pH 7, 3,200 g, 10 min, 4 °C) and
stabilized by adding 2 ml paraformaldehyde solution (PFA, 2% in PBS) to the cell pellet and incubated
for 30 min at room temperature (RT). After another centrifugation step (3,200 g, 10 min, 4 °C), 4 mL
of ethanol (70%) were added for fixation and the cell solution stored at -20 °C for maximum 6 month.

Step 2: DNA staining

The stored samples were washed twice (3,200 g, 10 min, 4 °C ) with PBS, and cell solutions were
adjusted to an OD of 0.035 (dʎ700nm = 5 mm) with PBS. 2 mL of an adjusted sample were centrifuged
(3,200 g, 10 min, 4 °C), and the pellet resuspended with 1 mL solution A (0.11 M citric acid and 4.1
mM Tween 20, with distilled water) and incubated at RT for 10 min in an ultrasonication bath (Merck
Eurolab, Darmstadt, Germany) and 10 min without any further treatment. After another
centrifugation step (3,200 g, 10 min, 4 °C) the cells were stained with solution B [0.24 μM DAPI (4',6-
diamidino-2-phenylindole) in phosphate buffer (289 mM Na2HPO4 and 128 mM NaH2PO4 in distill
water)] overnight at RT.

2. Preparation of cells for determination of cell numbers

Step 1: Fixation
For counting cell numbers, 1 mL samples were taken from the bioreactors and centrifuged (21,000 g,
10 min, 4 °C), the supernatant discarded and the pellets frozen at -20 °C.

Step 2: SYBR Green I staining for cell counting

The frozen pellets were thawed at RT for 5 min and resuspended in 1 mL PBS, and 100 µL of this
solution were diluted with 900 µL PBS. 50 µL of the diluted sample were transferred into glass tubes
containing 670 µL PBS, and 40 µL absolute Ethanol (99.5%, Chemsolute®, Th. Geyer GmbH,
Renningen, Germany) and 40 µL SYBR Green I solution (10,000 × stock, first diluted to 200 × with
DMSO, then diluted to 10 × with Millipore water; Eugene, Oregon, US) were added and incubated
overnight at RT. With this procedure, a 200 fold dilution rate was determined for cell counting (f =
200, Equation S5.1).

4
S5: Cytometric analysis
1: Measurement of community composition
Step 1: Instrumental set up
The cells were flow cytometrically analyzed with a BD Influx v7 Sorter USB applying the BD FACS
Software 1.2.0.142 (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). The instrument was
equipped with a 488 nm Sapphire OPS laser (400 mW) and a 355 nm Genesis CX laser (100 mW, both
Coherent, Santa Clara, CA, USA). The 488 nm laser light was used for the detection of the forward
scatter (FSC, 488/10), the side scatter (SSC, 488/10, trigger signal), and the SYBR Green I fluorescence
(PMT3, 540/30). The DAPI fluorescence was measured at PMT9 (460/50) after excitation with 355 nm
laser light.
The fluidic system was run at 33 psi using a 70 µm nozzle. The sheath fluid consisted of 0.5 x
FACSFlow buffer (BD). For the daily optical calibration of the cytometer in the linear range, 1 µm blue
fluorescent FluoSpheres (Molecular Probes, F-8815, Eugene, OR, USA) and 2 µm yellow-green
fluorescent FluoSpheres (ThermoFisher Scientific, F8827, Waltham, MA, USA) were used. For
calibration in the logarithmic range, 0.5 µm UV Fluoresbrite Microspheres (Polysciences, 18339,
Warrington, PA, USA) were applied.
Step 2: Measuring cell samples
Prior to measurement, DAPI stained cells were spiked with 0.5 µm and 1 µm UV Fluoresbrite
Microspheres (both Polysciences, 18339 and 17458, Warrington, PA, USA). The microspheres served
as internal standards to monitor instrument stability and to allow the correct comparison of samples
(Beads, Fig. S5.1). Cell data were collected in logarithmically scaled 2D-dot plots according to DAPI
fluorescence for DNA content and forward scatter (FSC) for cell size related information. A master
gate was defined which comprised 200,000 virtual cells for each measurement (Fig. S5.1 A).
Step 3: Creation of the gate-template to determine community structure
According to measured samples, apparent cell clusters in 2D-dot plots (FSC and DAPI fluorescence)
were gated sample per sample (Koch et al., 2013) and all defined gates were combined together to
create the gate-template. In this study, the gate-template included 68 gates (G1 to G68; Fig. S5.1 B).
Step 4: Cell sorting
The cell sorting procedure was done according to the protocol of Lambrecht et al. (2017). Briefly, the
positions of gates (subcommunities) to be sorted were defined and manually assigned to the BD
FACS Software. Cell sorting was done using the ‘1.0 Drop Pure’ sort mode. Between 500,000 and
1,400,000 cells of selected gates were sorted into tubes at rates from 200 up to 1,000 cells per sec.
Sorted cells were harvested by two successive centrifugation steps (20,000 g, 6 °C, 20 min), and the
cell pellets were stored at -20 °C for subsequent DNA isolation and MiSeq analysis.

5
Figure S5.1: Exemplary 2D-dot plot (FSC and DAPI fluorescence) of a cell sample taken from C1 at day 49 which
was fixated, stained with DAPI and measured according to the described workflow. A: A master gate was set
that contained 200,000 cells (grey) while beads, used for alignment, were indicated with arrows. B: a master
gate-template was created with 68 gates (1 to 68), two further gates contained the alignment beads (grey).

2. Measurement of cell counts

Step 1: Instrumental set up
The set up procedure is the same as for the measurement of community composition.
Step 2: Determination of cell number
A defined volume of SYBR Green I stained cells was mixed with a defined volume of 1 µm yellow-
green fluorescent beads of known concentration (F-13081, Molecular Probes, Eugene, OR, USA).
SYBR Green I fluorescence was excited with 488 nm laser light and detected at PMT3 (540/30). After
removing instrumental noise with a ‘Parent gate’ in a 2D-dot scatter plot (FSC and SSC, Fig. S5.2 A),
the 2D-dot plot (FSC and SYBR Green I fluorescence, Fig. S5.2 B) was gated with regard to cells (‘Cell
count’) and beads (‘1.0 µm YG beads’). Cell counts were calculated according to Equation S5.1.

𝑓∙𝐶(count)∙𝐵∙𝑉
Equation S5.1: Number of cells mL−1 = 𝐵(YG)∙𝑉(stain)

f: The dilution rate of sample for counting, 200 fold in this study
C: The virtual cell number in the gate ‘Cell count’
B: The defined concentration of 1 µm YG beads
V: The volume of defined concentration 1 µm YG beads
B(YG): The number of beads in the gate ‘1.0 µm YG beads’
V(stain): The defined volume of SYBR Green I stained cells sample

6
Figure S5.2: Exemplary 2D-dot plots of sample 49 d from C1, stained with SYBR Green I. A: The ‘Parent gate’
was defined in a FSC/SSC 2D-plot, which removed instrumental noise. B: The gates ‘Cell count’ and ‘1.0 µm YG
beads’ were defined on the basis of the ‘Parent gate’ and used for cell counting.

Reference
Koch, C., Günther, S., Desta, A. F., Hübschmann, T., and Müller, S. (2013) Cytometric fingerprinting
for analyzing microbial intracommunity structure variation and identifying subcommunity function.
Nat Protoc 8: 190-202.
Lambrecht, J., Cichocki, N., Hübschmann, T., Koch, C., Harms, H., and Müller, S. (2017) Flow
cytometric quantification, sorting and sequencing of methanogenic archaea based on F 420
autofluorescence. Microb Cell Fact 16: 180.

7
S6: Reproducibility of cytometric measurements
To verify the reliability of cytometric measurements in this study, a sample from the C2 was taken at
day 5. The sample was divided into three parts and treated separately according to the workflow in
S4. As an outcome three parallel 2D-dot plots (FSC and DAPI fluorescence) were produced (M1-M3,
Fig. S6.1 A). The master gate-template (Fig. S5.1 B) was used to count cell abundances per gate using
FlowJo (V10, FlowJo LLC, Oregon, USA). The mean value of relative cells abundances per gate (%, as
fraction of the sum of cell abundances of all gates) varied between 16.64% (G15) and 0.015% (G58,
Fig. S6.1 B). Within the three replicates the variation of the relative cells abundance per gate was low
with standard deviations between 0.002% and 0.74% for gates G53 and G15, respectively (Fig. S6.1
B).

Figure S6.1: A: Cytometric 2D-dot plots (FSC and DAPI fluorescence) of triplicate measurements (M1 - M3) of a
sample taken from bioreactor C2 at day 5. The master gate-template (Fig. S5.1 B) was shown as an overlay. B:
Relative cell abundances per gate (G1 to G68, left to right) in triplicate measurements (M1, M2 and M3) were
shown with different colors (blue, red, and green, respectively), and the statistical analysis as mean value (black
rect) and standard deviation (black bar with cap) add as overlays.

8
S7: Data lists for biostatistical evaluation of cytometric data
1. Data lists of cells abundance per subcommunity per sample
For a sample, the relative cells abundance per subcommunity was defined as the proportion (%) of
cells per subcommunity to the sum of cells in all subcommunities. In this study, community
structures of 326 samples (one for the inoculum and 325 for cell samples taken from five reactors)
were determined by the described workflows in S4 and S5. The data list of relative cells abundances
of all subcommunities (68 gates × 326 samples) are given in Table S7.1-5. While the highest value
was 48.78% (G36 in C1 at 0.25 d) , the lowest value was 0% (e.g. G27 in C1 at 0.25 d) which means
the relative subcommunity was not present in a sample.

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Table S7.1: Overview of relative cells abundance per subcommunity (%) in samples taken from preculture (inoculum) and reactor C1 (G1 to G68). Reactors: Inoculum (IN), C1. Time: time points of sampling (day).
Reacto r Time G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 G13 G14 G15 G16 G17 G18 G19 G20 G21 G22 G23 G24 G25 G26 G27 G28 G29 G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G40 G41 G42 G43 G44 G45 G46 G47 G48 G49 G50 G51 G52 G53 G54 G55 G56 G57 G58 G59 G60 G61 G62 G63 G64 G65 G66 G67 G68
IN 0 0.084 0.029 0.134 0.213 0.797 0.172 0.247 0.633 0.118 0.041 0.078 0.319 1.470 4.498 0.162 0.221 0.107 0.550 0.078 0.045 1.007 0.047 0.051 0.230 0.191 0.022 0.010 0.409 0.230 0.034 0.252 0.025 0.022 1.132 0.700 33.360 11.116 6.206 19.460 5.483 3.451 0.306 0.268 0.879 0.343 0.193 0.014 0.041 0.006 1.042 0.118 0.180 0.380 0.032 0.039 0.297 0.383 0.464 0.036 0.526 0.069 0.014 0.281 0.016 0.052 0.485 0.098 0.005
C1 0.25 0.004 0.004 0.057 0.016 0.374 0.004 0.075 0.067 0.004 0.001 0.001 0.042 0.931 1.630 0.018 0.019 0.055 0.239 0.004 0.006 0.172 0.004 0.006 0.011 0.013 0.005 0.000 0.015 0.060 0.082 0.045 0.026 0.006 0.119 0.103 48.775 18.696 12.513 3.922 7.248 0.970 0.006 0.004 0.949 0.077 0.040 0.001 0.048 0.006 0.045 0.013 0.016 0.515 0.003 0.003 0.435 0.096 0.172 0.004 0.685 0.001 0.001 0.06 0.00 0.01 0.32 0.15 0.00
C1 1 0.089 0.005 0.059 0.116 0.233 1.720 0.268 3.843 0.348 0.006 0.264 1.047 4.960 5.733 2.557 3.139 0.584 1.245 0.059 0.012 2.035 0.607 0.031 1.099 0.340 0.028 0.012 1.705 0.538 0.048 0.311 0.015 0.003 2.479 0.951 22.117 5.349 2.277 8.329 8.663 2.727 2.999 1.704 1.043 0.539 0.494 0.116 0.024 0.005 1.676 0.037 0.082 1.316 0.004 0.374 0.212 0.569 0.203 0.158 0.884 0.597 0.001 0.23 0.00 0.14 0.31 0.31 0.02
C1 2 0.115 0.011 1.921 2.473 0.899 3.737 0.785 2.951 2.188 0.061 0.525 1.314 3.001 3.485 18.201 5.067 0.257 0.919 2.953 0.285 0.695 5.298 0.022 2.899 0.198 0.517 0.150 0.776 0.461 0.203 0.336 0.016 0.002 1.250 0.451 5.946 1.941 0.704 3.343 1.717 1.627 4.574 5.360 0.351 0.223 0.632 0.603 0.782 0.005 2.212 0.961 0.194 0.336 0.009 1.636 0.063 0.380 0.088 0.170 0.323 0.329 0.005 0.20 0.01 0.28 0.24 0.26 0.07
C1 3 0.084 0.034 3.805 1.884 2.203 7.838 1.873 4.085 0.721 0.132 0.703 1.255 3.178 4.275 15.560 6.665 0.445 1.728 2.502 0.991 0.225 5.538 0.041 1.236 0.226 1.173 0.402 1.168 0.339 0.588 0.383 0.031 0.023 1.325 0.710 1.870 0.623 0.336 2.733 0.662 1.429 4.616 3.910 0.742 0.472 0.582 1.370 1.746 0.012 1.211 1.175 0.151 0.155 0.025 0.808 0.084 0.122 0.027 0.211 0.164 0.750 0.007 0.13 0.02 0.15 0.10 0.19 0.06
C1 5 1.027 0.750 5.254 2.885 1.530 0.876 0.911 11.543 2.434 1.172 0.216 0.506 4.250 0.977 17.883 4.931 0.272 1.532 2.102 1.409 0.535 1.146 0.357 8.602 0.776 0.276 0.158 0.539 0.090 0.786 0.109 0.345 0.045 2.434 0.467 0.841 0.265 0.162 1.083 0.334 1.007 1.935 2.390 2.073 1.545 0.392 0.120 0.621 0.300 3.394 0.876 0.058 0.293 0.406 0.405 0.292 0.019 0.009 0.133 0.237 0.235 0.264 0.25 0.51 0.10 0.13 0.17 0.02
C1 7 1.337 2.576 20.238 6.549 0.929 1.178 0.777 0.720 2.319 3.705 0.323 0.352 1.126 0.639 2.537 1.167 0.618 1.147 3.360 10.442 0.264 1.550 0.397 2.342 0.900 0.361 0.244 1.257 0.315 0.228 1.147 0.079 0.332 2.016 0.913 0.256 0.072 0.045 0.651 0.122 0.568 1.045 1.134 0.151 0.102 0.220 0.302 0.808 9.242 1.748 0.832 1.045 0.048 3.129 0.305 0.196 0.028 0.008 0.501 0.026 0.411 0.421 0.05 0.83 0.09 0.34 0.67 0.23
C1 8 0.926 0.991 20.905 6.276 4.312 0.576 0.922 0.937 3.652 4.640 0.200 0.463 1.646 0.853 5.347 2.291 0.257 0.808 3.626 6.560 0.360 1.215 0.053 3.604 0.713 0.201 0.065 0.488 0.172 0.223 1.423 0.074 0.051 1.023 0.438 0.332 0.082 0.054 0.966 0.188 0.734 1.532 2.257 0.176 0.069 0.142 0.116 0.954 5.480 2.039 0.578 2.654 0.051 2.491 0.604 0.193 0.077 0.009 0.102 0.047 0.176 0.320 0.04 0.98 0.05 0.08 0.12 0.01
C1 9 0.706 0.704 17.074 4.725 4.792 0.336 0.472 0.740 3.569 4.468 0.110 0.487 1.627 1.092 8.924 3.498 0.120 0.732 3.844 6.770 0.284 3.582 0.020 4.803 0.574 0.071 0.019 0.239 0.129 0.141 1.010 0.047 0.011 0.831 0.222 0.558 0.113 0.076 1.429 0.277 1.212 1.996 2.351 0.069 0.026 0.135 0.051 0.135 5.666 2.471 0.212 1.981 0.031 2.030 0.904 0.128 0.034 0.007 0.031 0.030 0.110 0.191 0.04 0.77 0.09 0.02 0.05 0.01
C1 10 0.298 0.599 20.468 6.652 2.366 0.280 0.512 0.995 2.371 4.761 0.100 0.547 1.764 0.942 10.876 2.643 0.102 0.391 3.181 10.979 0.626 2.776 0.016 3.608 0.253 0.054 0.016 0.183 0.145 0.183 0.770 0.058 0.023 0.432 0.173 0.348 0.077 0.054 1.008 0.203 0.585 1.547 2.445 0.080 0.040 0.179 0.054 0.180 6.639 1.300 0.237 1.080 0.053 1.807 0.930 0.119 0.025 0.007 0.039 0.039 0.080 0.101 0.05 0.40 0.08 0.01 0.05 0.01
C1 11 0.190 0.284 23.229 5.980 1.174 0.359 0.342 1.293 2.426 3.699 0.203 0.527 1.440 0.853 17.818 1.819 0.076 0.288 3.344 7.318 0.352 3.227 0.031 4.330 0.245 0.053 0.008 0.181 0.231 0.256 0.466 0.094 0.021 0.386 0.106 0.352 0.081 0.062 1.154 0.237 0.552 1.739 3.308 0.129 0.046 0.294 0.075 0.147 3.847 1.282 0.316 0.524 0.075 1.039 1.204 0.064 0.015 0.003 0.029 0.095 0.120 0.061 0.07 0.26 0.08 0.03 0.05 0.02
C1 12 0.473 0.336 9.726 3.377 0.653 0.966 0.357 2.478 1.601 1.454 0.346 1.332 1.731 0.991 23.684 3.777 0.077 0.436 2.756 3.938 0.461 4.771 0.112 6.595 0.504 0.152 0.025 0.246 0.718 0.863 0.353 0.832 0.054 1.271 0.146 0.660 0.203 0.139 1.197 0.321 0.701 3.403 5.119 0.177 0.083 0.954 0.119 0.148 1.813 2.926 0.260 0.227 0.092 0.675 1.861 0.102 0.015 0.007 0.030 0.247 0.313 0.085 0.20 0.12 0.13 0.02 0.04 0.01
C1 14 0.568 0.477 9.196 3.568 3.623 2.464 1.914 5.562 1.948 1.009 0.691 4.520 3.097 1.745 6.942 2.699 0.272 1.498 1.958 2.980 1.294 1.937 0.073 4.087 0.324 1.047 0.065 0.401 3.261 1.687 1.247 1.592 0.048 1.719 0.472 1.843 0.643 0.438 2.001 0.755 1.564 1.392 2.319 0.320 0.380 3.085 0.588 0.762 1.361 2.210 0.580 0.519 0.191 0.470 0.687 0.134 0.009 0.002 0.093 0.368 0.217 0.074 0.33 0.19 0.36 0.02 0.08 0.02
C1 15 0.790 0.624 5.861 2.593 3.545 1.235 2.337 8.596 1.553 0.832 0.222 5.537 3.633 1.312 8.216 2.959 0.229 1.236 2.411 2.357 1.175 4.119 0.151 3.181 0.336 0.743 0.184 0.341 6.333 1.325 2.201 1.832 0.019 1.231 0.427 0.972 0.297 0.226 1.321 0.426 1.034 1.318 1.884 0.264 0.328 3.280 0.206 0.561 1.456 1.724 0.549 1.367 0.101 0.415 0.781 0.058 0.013 0.002 0.069 0.175 0.092 0.095 0.40 0.26 0.54 0.03 0.06 0.02
C1 17 0.349 0.227 2.977 1.353 2.604 0.445 3.082 15.888 0.764 0.391 0.082 5.949 5.003 1.618 6.425 2.979 0.056 0.445 1.442 1.269 2.476 3.452 0.050 1.732 0.178 0.151 0.013 0.102 11.527 1.050 5.527 1.331 0.020 0.507 0.144 1.077 0.358 0.232 1.319 0.536 0.769 0.969 1.153 0.541 0.462 1.973 0.082 0.587 0.635 1.074 0.309 3.341 0.202 0.144 0.543 0.054 0.030 0.005 0.009 0.263 0.046 0.031 1.01 0.07 0.52 0.01 0.03 0.00
C1 18 0.787 0.165 3.486 1.562 1.168 1.263 2.237 15.232 1.182 0.419 0.168 4.507 6.679 1.778 5.399 2.603 0.163 0.723 1.486 1.428 4.513 3.428 0.066 2.234 0.381 0.310 0.025 0.240 3.952 1.968 7.951 2.067 0.600 0.756 0.311 1.188 0.360 0.261 1.890 0.523 1.251 1.332 1.166 0.569 0.910 1.086 0.290 0.484 0.297 1.643 0.198 2.924 0.174 0.105 0.570 0.128 0.031 0.015 0.046 0.161 0.121 0.034 0.45 0.13 0.27 0.06 0.09 0.01
C1 19 0.509 0.228 3.231 2.016 1.090 0.873 2.823 21.508 1.063 0.377 0.112 4.342 6.471 1.110 8.468 4.601 0.148 0.696 1.411 1.241 3.624 2.130 0.124 1.441 0.218 0.267 0.080 0.208 2.357 1.289 6.841 2.039 0.036 0.708 0.315 0.846 0.316 0.264 1.009 0.327 1.000 1.114 1.585 0.283 0.385 1.178 0.182 0.135 0.331 1.197 0.307 3.363 0.062 0.138 0.436 0.081 0.035 0.015 0.042 0.086 0.073 0.031 0.26 0.20 0.54 0.09 0.09 0.01
C1 21 0.645 0.133 4.476 2.020 1.041 0.719 1.631 28.683 0.726 0.335 0.113 5.893 7.220 0.689 7.096 3.938 0.087 0.399 0.706 1.618 5.567 0.738 0.149 0.663 0.195 0.117 0.045 0.136 2.101 1.640 5.545 1.520 0.016 0.465 0.169 0.482 0.183 0.160 0.602 0.150 0.552 0.844 0.929 0.276 0.518 0.971 0.125 0.498 0.232 0.886 0.338 3.424 0.041 0.122 0.189 0.063 0.067 0.025 0.019 0.048 0.069 0.018 0.21 0.11 0.46 0.06 0.08 0.01
C1 22 0.883 0.473 5.928 3.030 0.587 0.557 0.763 32.273 0.602 0.576 0.157 5.157 6.845 0.652 8.019 4.217 0.037 0.220 0.665 2.012 5.248 0.547 0.214 0.469 0.115 0.186 0.050 0.052 1.254 2.202 3.177 3.947 0.021 0.171 0.082 0.219 0.057 0.052 0.487 0.086 0.354 0.783 1.370 0.175 0.399 0.496 0.084 0.169 0.599 0.627 0.238 1.066 0.041 0.231 0.167 0.058 0.038 0.016 0.010 0.050 0.021 0.024 0.17 0.23 0.28 0.01 0.01 0.00
C1 23 0.774 0.554 5.677 2.889 0.657 0.553 0.664 33.526 0.628 0.600 0.107 5.276 6.464 0.548 9.447 4.904 0.044 0.207 0.623 1.747 4.709 0.436 0.214 0.513 0.101 0.240 0.068 0.056 1.121 1.442 3.009 3.355 0.014 0.143 0.086 0.231 0.063 0.063 0.436 0.074 0.332 0.880 1.655 0.120 0.293 0.386 0.082 0.107 0.563 0.538 0.208 1.047 0.032 0.300 0.142 0.082 0.043 0.007 0.011 0.022 0.029 0.043 0.15 0.27 0.35 0.01 0.03 0.00
C1 24 0.553 0.466 4.053 2.251 0.868 0.513 0.415 39.034 0.474 0.436 0.117 6.911 7.446 0.646 8.208 5.104 0.054 0.241 0.584 1.136 4.618 0.537 0.102 0.463 0.085 0.134 0.039 0.074 0.707 1.310 1.389 2.144 0.003 0.160 0.098 0.363 0.131 0.109 0.560 0.121 0.448 0.939 1.780 0.196 0.336 0.232 0.078 0.126 0.263 0.528 0.139 0.834 0.046 0.202 0.160 0.099 0.069 0.010 0.013 0.069 0.046 0.053 0.22 0.20 0.20 0.02 0.03 0.00
C1 25 0.451 0.457 4.248 2.541 1.122 0.418 0.614 40.291 0.591 0.518 0.102 4.768 8.142 0.661 8.716 4.149 0.037 0.187 0.740 1.171 5.748 0.596 0.095 0.526 0.102 0.064 0.033 0.077 0.660 0.870 1.637 1.117 0.003 0.135 0.069 0.327 0.115 0.114 0.498 0.115 0.377 0.864 1.321 0.199 0.515 0.218 0.063 0.297 0.202 0.522 0.205 0.812 0.035 0.236 0.153 0.149 0.058 0.010 0.013 0.067 0.046 0.072 0.21 0.26 0.23 0.01 0.03 0.00
C1 28 0.205 0.954 4.728 2.273 1.817 0.146 0.603 41.237 0.529 0.605 0.061 6.377 6.793 0.466 9.002 4.495 0.032 0.134 0.480 1.027 5.926 0.546 0.044 0.481 0.114 0.026 0.022 0.040 0.232 0.818 0.315 2.269 0.004 0.101 0.060 0.280 0.101 0.079 0.320 0.098 0.287 0.705 1.506 0.149 0.531 0.203 0.027 0.053 0.536 0.335 0.151 0.116 0.030 0.425 0.133 0.052 0.038 0.016 0.009 0.034 0.030 0.115 0.13 0.32 0.20 0.01 0.02 0.00
C1 29 0.139 0.561 6.292 3.800 2.581 0.100 0.882 37.985 0.682 0.818 0.048 7.330 6.578 0.462 6.314 4.240 0.032 0.138 0.715 1.801 5.674 0.412 0.074 0.378 0.097 0.015 0.011 0.033 0.242 1.037 0.283 2.601 0.006 0.131 0.058 0.479 0.170 0.144 0.296 0.126 0.335 0.552 1.396 0.213 0.547 0.286 0.004 0.075 0.497 0.346 0.273 0.106 0.036 0.277 0.119 0.053 0.054 0.027 0.007 0.135 0.036 0.035 0.14 0.37 0.30 0.00 0.01 0.00
C1 30 0.117 0.354 6.758 4.065 4.460 0.072 1.441 33.452 0.896 0.719 0.061 7.325 6.171 0.981 6.410 3.727 0.024 0.154 1.005 2.099 5.035 0.739 0.078 0.558 0.139 0.021 0.012 0.036 0.286 1.135 0.181 2.726 0.007 0.193 0.091 0.637 0.230 0.161 0.559 0.208 0.343 0.492 1.187 0.428 0.624 0.388 0.005 0.140 0.451 0.412 0.417 0.086 0.105 0.217 0.192 0.085 0.032 0.014 0.004 0.183 0.018 0.034 0.14 0.24 0.43 0.00 0.00 0.00
C1 31 0.102 0.323 4.887 5.157 4.969 0.066 1.757 33.897 1.233 0.942 0.040 6.764 6.232 0.612 7.369 4.093 0.021 0.097 1.400 2.341 6.097 0.673 0.036 0.658 0.106 0.010 0.015 0.032 0.230 0.595 0.163 1.201 0.004 0.109 0.052 0.509 0.228 0.154 0.340 0.161 0.286 0.654 1.396 0.266 0.527 0.368 0.004 0.225 0.324 0.432 0.460 0.054 0.056 0.197 0.146 0.035 0.025 0.009 0.007 0.129 0.017 0.034 0.09 0.31 0.26 0.00 0.01 0.00
C1 35 0.286 0.820 8.506 17.144 8.741 0.185 1.887 10.210 3.125 1.359 0.035 5.770 3.419 1.451 4.733 2.702 0.042 0.218 3.026 2.062 1.113 0.696 0.012 1.285 0.178 0.025 0.027 0.061 0.479 6.307 0.089 2.827 0.019 0.260 0.068 0.446 0.145 0.128 1.225 0.285 0.567 0.832 1.221 0.319 0.296 0.420 0.017 0.354 0.564 0.962 0.279 0.038 0.098 0.412 0.238 0.589 0.009 0.002 0.009 0.060 0.064 0.085 0.08 0.68 0.32 0.04 0.04 0.00
C1 36 0.233 0.326 7.188 17.066 9.355 0.281 2.287 12.709 3.727 0.984 0.056 6.468 3.709 1.397 3.382 2.337 0.038 0.214 3.495 1.943 0.940 0.499 0.025 1.400 0.162 0.073 0.047 0.110 0.472 5.378 0.289 0.275 0.007 0.267 0.066 0.444 0.118 0.101 1.285 0.311 0.572 0.746 1.138 0.367 0.409 0.673 0.022 1.398 0.118 0.972 0.622 0.448 0.088 0.197 0.235 1.198 0.007 0.002 0.010 0.088 0.075 0.033 0.13 0.55 0.34 0.06 0.04 0.00
C1 37 0.435 1.164 2.968 16.132 11.571 0.358 2.798 17.671 3.575 1.048 0.086 6.961 3.546 1.224 3.374 1.702 0.075 0.345 2.927 1.275 1.103 0.417 0.044 1.648 0.290 0.118 0.064 0.131 0.376 2.360 0.415 0.072 0.004 0.379 0.120 0.619 0.198 0.152 1.147 0.295 0.673 0.613 0.952 0.430 0.485 0.650 0.039 0.798 0.151 1.118 0.363 0.545 0.090 0.363 0.226 1.292 0.017 0.002 0.015 0.063 0.082 0.222 0.37 0.47 0.44 0.20 0.13 0.01
C1 38 0.716 2.306 2.361 17.350 10.774 0.394 2.635 20.029 3.773 1.311 0.106 5.551 4.098 1.130 2.993 1.712 0.086 0.327 3.027 1.635 1.390 0.384 0.054 1.419 0.349 0.088 0.066 0.148 0.425 0.278 0.417 0.081 0.003 0.372 0.132 0.593 0.140 0.123 1.024 0.266 0.604 0.583 1.055 0.451 0.478 0.622 0.067 0.123 0.336 0.955 0.260 0.539 0.105 0.667 0.218 0.166 0.022 0.002 0.030 0.084 0.066 0.652 0.27 0.68 0.41 0.31 0.17 0.02
C1 39 0.498 1.429 8.433 24.009 8.111 0.460 1.674 9.931 3.448 1.871 0.114 3.639 4.288 1.750 2.412 1.791 0.141 0.451 3.067 3.076 0.704 0.469 0.027 1.310 0.276 0.082 0.046 0.143 0.325 0.428 0.353 0.055 0.002 0.294 0.184 0.632 0.142 0.121 1.776 0.328 1.061 1.053 1.671 0.234 0.173 0.493 0.102 0.477 0.269 1.350 0.294 0.575 0.064 0.463 0.245 0.107 0.030 0.003 0.034 0.080 0.087 0.181 0.06 1.68 0.30 0.34 0.26 0.02
C1 43 0.900 1.332 7.498 27.342 7.311 1.377 1.463 11.528 4.032 1.554 0.610 3.823 2.499 0.443 1.612 0.829 0.179 0.274 3.829 5.519 1.312 0.360 0.465 1.216 0.392 0.921 0.311 0.159 0.253 0.895 0.073 0.420 0.007 0.384 0.169 0.159 0.042 0.044 0.371 0.059 0.364 0.306 0.640 0.101 0.125 0.343 0.181 0.716 0.253 0.583 0.462 0.043 0.014 0.294 0.199 0.257 0.014 0.006 0.074 0.005 0.105 0.139 0.85 1.04 0.12 0.42 0.36 0.02
C1 44 0.769 1.595 4.557 24.774 10.286 0.975 2.203 15.799 4.601 1.121 0.364 3.455 2.962 0.432 1.527 0.960 0.143 0.274 3.656 3.459 1.559 0.314 0.414 1.387 0.377 0.843 0.384 0.228 0.306 0.690 0.082 0.520 0.010 0.403 0.188 0.099 0.023 0.031 0.313 0.043 0.282 0.365 0.790 0.105 0.242 0.411 0.112 0.183 0.297 0.690 0.415 0.055 0.008 0.469 0.184 0.174 0.020 0.006 0.098 0.003 0.120 0.373 0.65 0.48 0.18 0.71 0.41 0.04
C1 45 0.956 0.947 2.123 18.895 14.568 1.564 3.967 12.252 5.327 0.625 0.522 4.023 5.827 0.719 1.445 1.220 0.166 0.501 3.490 2.191 1.777 0.464 0.430 1.889 0.519 0.899 0.400 0.379 0.465 0.222 0.176 0.169 0.006 0.723 0.244 0.170 0.038 0.045 0.553 0.064 0.517 0.489 1.065 0.129 0.216 0.537 0.274 0.249 0.160 1.135 0.621 0.208 0.019 0.237 0.215 0.123 0.019 0.010 0.097 0.010 0.208 0.307 0.65 0.27 0.29 0.55 0.40 0.04
C1 46 1.702 0.181 3.814 6.531 14.703 6.265 3.653 5.700 2.612 0.510 1.611 2.514 3.866 0.742 2.031 0.561 0.184 0.359 1.260 0.898 2.496 0.921 1.260 1.001 0.554 2.947 1.526 0.432 0.684 0.417 2.719 0.557 0.007 0.808 0.229 0.755 0.139 0.100 0.719 0.454 0.580 0.264 0.792 3.511 4.613 0.422 1.572 0.304 0.083 0.437 0.396 2.473 0.181 0.082 0.359 0.230 0.044 0.007 0.162 0.074 0.484 0.035 2.78 0.49 0.23 0.41 0.46 0.09
C1 49 1.452 0.594 1.840 10.184 29.556 2.469 5.771 1.916 1.608 0.468 1.150 2.776 0.869 0.286 1.472 1.360 0.332 0.552 2.133 4.993 0.438 0.337 0.891 0.749 0.619 2.139 1.135 1.462 1.545 0.208 2.441 0.124 0.104 1.092 0.424 0.196 0.050 0.033 0.222 0.080 0.314 0.255 0.412 0.335 0.334 0.889 0.428 0.139 0.090 0.685 0.846 4.282 0.032 0.215 0.176 0.064 0.031 0.011 0.274 0.036 0.554 0.193 0.53 0.39 0.31 1.17 0.80 0.13
C1 50 0.843 1.439 0.933 31.460 19.870 0.500 5.362 0.853 4.972 0.994 0.342 0.975 0.647 0.361 1.013 0.784 0.156 0.522 5.355 6.906 0.244 0.475 0.328 2.257 0.715 0.077 0.047 0.333 0.877 0.033 0.903 0.042 0.051 0.802 0.236 0.192 0.042 0.029 0.361 0.077 0.397 0.422 0.471 0.121 0.081 0.624 0.068 0.051 0.096 1.258 0.701 1.065 0.039 0.263 0.174 0.037 0.012 0.005 0.071 0.040 0.154 0.083 0.05 0.75 0.22 0.13 0.17 0.02
C1 51 0.542 0.206 1.390 18.459 37.872 0.375 7.484 0.701 4.591 0.499 0.097 0.685 0.599 0.375 0.696 0.591 0.110 0.419 4.233 6.396 0.272 0.618 0.208 2.412 0.507 0.043 0.011 0.194 0.800 0.054 0.271 0.024 0.007 0.631 0.155 0.273 0.074 0.054 0.459 0.104 0.477 0.521 0.503 0.143 0.133 0.414 0.045 0.088 0.027 1.253 1.157 0.417 0.036 0.049 0.228 0.051 0.010 0.005 0.028 0.037 0.094 0.037 0.09 0.41 0.15 0.03 0.07 0.01
C1 52 0.438 0.278 1.475 22.662 31.770 0.369 5.749 0.408 6.540 0.667 0.131 0.391 0.473 0.273 0.788 0.515 0.123 0.566 5.633 6.254 0.124 0.690 0.406 3.317 0.574 0.062 0.022 0.241 0.212 0.087 0.099 0.031 0.004 0.847 0.222 0.182 0.045 0.035 0.381 0.059 0.477 0.624 0.616 0.128 0.115 0.286 0.051 0.072 0.043 1.704 1.046 0.157 0.014 0.117 0.295 0.126 0.013 0.004 0.037 0.013 0.114 0.035 0.02 0.47 0.12 0.06 0.09 0.01
C1 53 0.695 0.327 1.295 20.041 30.739 0.554 4.133 0.695 5.749 0.633 0.214 0.432 0.493 0.270 1.658 0.716 0.126 0.572 5.773 8.512 0.147 1.120 0.813 3.138 0.671 0.111 0.032 0.306 0.132 0.142 0.052 0.076 0.012 0.904 0.248 0.187 0.051 0.044 0.371 0.063 0.517 0.593 0.728 0.154 0.164 0.219 0.085 0.092 0.057 1.605 1.742 0.106 0.014 0.072 0.329 0.325 0.017 0.003 0.047 0.006 0.137 0.043 0.03 0.35 0.11 0.09 0.10 0.01
C1 56 1.329 0.560 1.020 24.721 9.140 0.795 2.519 1.024 8.118 2.076 0.307 0.427 0.830 0.489 1.487 0.630 0.224 1.198 6.705 7.902 0.305 1.141 1.393 4.988 1.082 0.205 0.052 0.465 0.309 0.052 1.012 0.054 0.027 1.686 0.409 0.308 0.061 0.039 0.773 0.125 1.132 0.986 1.031 0.237 0.668 0.342 0.096 0.048 0.052 3.175 1.757 1.155 0.013 0.137 0.455 0.022 0.550 0.009 0.076 0.014 0.239 0.147 0.28 1.02 0.09 0.15 0.12 0.01
C1 57 0.593 0.533 1.680 19.695 6.840 0.484 1.309 1.305 7.830 1.988 0.283 0.474 1.196 0.608 1.570 0.702 0.220 1.005 8.480 15.218 0.472 1.350 0.953 5.589 1.024 0.183 0.047 0.506 0.201 0.040 0.355 0.023 0.050 1.422 0.424 0.260 0.045 0.032 0.746 0.102 0.887 0.976 1.013 0.215 0.657 0.184 0.056 0.087 0.112 3.128 3.425 0.404 0.018 0.092 0.451 0.019 0.233 0.009 0.104 0.006 0.222 0.084 0.62 0.74 0.08 0.16 0.14 0.03
C1 58 1.206 1.295 1.232 30.650 7.370 0.876 2.010 1.800 7.934 2.650 0.369 0.374 0.999 0.508 1.132 0.515 0.170 0.776 6.284 7.853 0.516 0.918 2.247 4.153 1.015 0.399 0.089 0.478 0.171 0.033 0.311 0.030 0.018 1.384 0.334 0.231 0.038 0.025 0.562 0.086 0.627 0.729 0.798 0.234 0.779 0.174 0.097 0.056 0.069 2.299 1.161 0.351 0.015 0.293 0.314 0.016 0.128 0.009 0.085 0.010 0.242 0.225 0.26 1.66 0.07 0.14 0.09 0.02
C1 59 0.985 2.157 1.460 25.487 7.664 0.646 1.642 1.868 7.639 2.897 0.315 0.405 1.171 0.583 1.515 0.590 0.194 1.015 5.846 9.750 0.563 1.131 0.950 4.789 1.125 0.169 0.041 0.429 0.136 0.046 0.155 0.037 0.049 1.551 0.421 0.354 0.063 0.047 0.764 0.141 0.976 0.905 0.936 0.246 0.939 0.157 0.061 0.081 0.176 3.059 1.671 0.163 0.030 0.328 0.404 0.035 0.059 0.003 0.058 0.013 0.234 0.274 0.59 1.43 0.10 0.18 0.10 0.01
C1 60 0.742 0.831 1.792 28.570 7.417 0.218 1.760 2.438 9.154 2.368 0.081 0.365 1.257 0.650 1.309 0.638 0.076 0.844 6.742 6.292 0.627 1.889 0.032 5.714 0.837 0.011 0.004 0.149 0.137 0.052 0.104 0.016 0.007 1.094 0.186 0.404 0.082 0.061 0.849 0.169 1.178 1.131 1.156 0.282 1.192 0.177 0.029 0.097 0.064 3.599 1.872 0.130 0.028 0.185 0.546 0.032 0.028 0.001 0.026 0.019 0.072 0.120 0.36 1.32 0.15 0.10 0.12 0.01
C1 65 8.616 0.334 2.953 23.429 2.623 1.543 0.749 10.518 5.310 0.285 1.597 3.246 2.959 0.985 1.037 1.187 0.123 0.593 5.013 6.793 1.355 0.908 0.382 2.078 1.049 0.155 0.019 0.225 0.229 0.054 0.047 0.012 0.081 0.938 0.182 0.504 0.121 0.079 1.211 0.236 0.906 0.658 0.747 0.325 0.496 0.181 0.052 0.199 0.032 1.478 2.986 0.072 0.064 0.046 0.368 0.024 0.009 0.004 0.038 0.046 0.149 0.094 0.77 0.16 0.24 0.03 0.06 0.01
C1 66 10.443 0.206 2.674 20.983 4.011 2.495 1.049 13.105 5.089 0.313 1.854 3.016 3.089 0.996 1.468 1.165 0.156 0.598 3.832 3.971 1.608 1.014 0.550 1.835 1.046 0.187 0.020 0.271 0.261 0.066 0.044 0.015 0.064 1.003 0.212 0.533 0.122 0.095 1.204 0.242 0.943 0.654 0.717 0.359 0.500 0.191 0.050 0.129 0.027 1.368 2.025 0.023 0.098 0.026 0.383 0.039 0.011 0.007 0.058 0.057 0.180 0.036 0.43 0.21 0.41 0.04 0.10 0.02
C1 67 5.563 0.659 2.440 18.799 4.034 2.083 0.956 19.716 3.769 0.318 1.117 3.977 4.839 1.207 1.865 2.152 0.162 0.521 3.424 3.760 2.446 0.660 1.383 1.236 0.711 0.204 0.027 0.258 0.204 0.073 0.145 0.025 0.046 0.742 0.220 0.500 0.116 0.077 1.087 0.241 0.845 0.604 1.027 0.440 0.394 0.228 0.053 0.107 0.091 1.039 1.023 0.369 0.098 0.154 0.288 0.028 0.016 0.006 0.082 0.058 0.203 0.118 0.40 0.13 0.18 0.08 0.14 0.03
C1 69 1.976 0.784 3.500 17.498 7.562 0.715 2.172 19.047 2.939 0.671 0.441 3.196 4.643 1.286 2.634 2.081 0.279 0.678 3.497 4.103 2.341 1.329 1.192 1.264 0.436 0.166 0.032 0.254 0.312 0.064 0.148 0.024 0.016 0.673 0.340 0.647 0.166 0.114 1.144 0.307 0.947 0.649 0.993 0.294 0.212 1.045 0.045 0.125 0.409 1.109 1.041 0.104 0.072 0.250 0.418 0.031 0.023 0.005 0.125 0.057 0.122 0.096 0.32 0.19 0.36 0.08 0.18 0.03
C1 70 2.125 0.773 3.978 16.259 11.018 0.774 2.017 15.419 2.725 0.930 0.471 2.911 4.090 1.244 1.840 1.501 0.275 0.675 3.708 7.044 2.064 1.195 1.058 1.454 0.587 0.198 0.067 0.394 0.250 0.084 0.098 0.035 0.052 0.857 0.350 0.515 0.128 0.085 1.075 0.225 0.862 0.596 0.744 0.296 0.295 0.385 0.070 0.155 0.465 1.124 1.794 0.032 0.069 0.225 0.310 0.032 0.013 0.002 0.141 0.040 0.176 0.102 0.60 0.31 0.17 0.15 0.25 0.04
C1 71 2.164 0.807 3.651 20.753 12.937 0.587 2.719 12.274 3.590 1.719 0.493 1.746 3.596 1.119 1.169 1.331 0.228 0.416 4.180 7.716 1.821 1.101 0.162 1.909 0.530 0.026 0.007 0.163 0.083 0.067 0.035 0.014 0.033 0.448 0.208 0.475 0.120 0.073 1.088 0.215 0.684 0.536 0.606 0.236 0.345 0.138 0.020 0.109 0.651 1.183 1.034 0.027 0.061 0.202 0.295 0.035 0.016 0.006 0.151 0.038 0.065 0.072 0.38 0.85 0.07 0.12 0.25 0.04
C1 72 2.926 0.312 4.033 18.650 16.276 0.706 3.049 9.669 4.143 1.141 0.628 1.675 2.996 1.113 1.014 1.019 0.154 0.454 3.938 6.100 2.042 0.933 0.046 1.941 0.581 0.015 0.006 0.177 0.198 0.044 0.048 0.012 0.011 0.619 0.203 0.514 0.129 0.074 1.061 0.295 0.662 0.546 0.586 0.455 1.834 0.200 0.018 0.158 0.501 1.216 1.921 0.033 0.117 0.063 0.310 0.025 0.010 0.012 0.067 0.076 0.092 0.024 1.27 0.41 0.14 0.11 0.18 0.02
C1 73 7.555 0.377 2.936 23.838 12.202 1.718 2.808 7.147 5.101 1.043 1.582 1.345 2.599 1.116 0.760 0.895 0.302 0.492 4.488 5.222 1.673 0.891 0.174 1.745 0.646 0.024 0.002 0.109 0.137 0.049 0.042 0.011 0.007 0.491 0.137 0.437 0.111 0.056 0.977 0.271 0.498 0.498 0.583 0.429 1.071 0.196 0.026 0.102 0.345 0.915 1.668 0.047 0.146 0.081 0.327 0.024 0.016 0.005 0.036 0.078 0.105 0.039 0.45 0.41 0.14 0.06 0.16 0.02
C1 74 7.051 0.371 2.721 16.482 18.639 1.621 4.084 9.124 3.742 1.115 1.545 1.827 2.936 1.062 0.818 1.105 0.172 0.457 3.349 3.844 2.229 0.703 0.384 1.724 0.700 0.105 0.009 0.203 0.189 0.039 0.036 0.012 0.029 0.690 0.203 0.608 0.157 0.109 1.136 0.312 0.737 0.462 0.483 0.422 1.390 0.178 0.035 0.083 0.190 1.091 1.001 0.028 0.115 0.082 0.268 0.025 0.010 0.008 0.090 0.085 0.117 0.050 0.45 0.47 0.09 0.14 0.21 0.04
C1 77 17.258 0.252 1.408 14.925 18.241 2.615 3.054 7.574 3.295 0.511 3.107 2.176 2.043 0.688 0.643 0.669 0.431 0.752 2.639 3.233 1.313 0.371 0.325 1.305 1.068 0.187 0.012 0.213 0.256 0.052 0.022 0.007 0.139 0.865 0.272 0.434 0.117 0.074 0.848 0.216 0.507 0.321 0.347 0.309 0.731 0.166 0.031 0.100 0.023 0.745 1.192 0.029 0.093 0.040 0.179 0.041 0.012 0.004 0.069 0.090 0.166 0.037 0.52 0.32 0.12 0.06 0.12 0.02
C1 78 6.723 0.455 0.967 13.324 22.192 2.024 4.255 10.927 3.918 0.706 1.582 2.067 2.741 0.808 0.695 0.767 0.370 0.694 2.310 2.316 1.881 0.383 0.477 1.871 1.026 0.180 0.108 0.795 0.232 0.034 0.025 0.015 0.035 1.220 0.455 0.446 0.102 0.062 0.894 0.253 0.659 0.460 0.432 0.385 1.746 0.147 0.201 0.069 0.039 1.290 0.619 0.022 0.128 0.071 0.244 0.039 0.013 0.007 0.334 0.107 0.357 0.057 0.60 0.41 0.08 0.40 0.59 0.17
C1 79 12.008 0.444 1.330 12.840 20.715 2.542 3.957 11.621 2.992 0.696 2.270 2.783 2.756 0.778 0.976 0.884 0.210 0.390 2.279 2.425 1.672 0.333 0.868 1.215 0.757 0.350 0.023 0.222 0.372 0.059 0.028 0.017 0.078 0.731 0.205 0.435 0.102 0.071 0.899 0.239 0.567 0.315 0.387 0.324 0.928 0.197 0.043 0.078 0.074 0.772 0.497 0.020 0.125 0.105 0.184 0.040 0.009 0.008 0.117 0.120 0.146 0.057 0.44 0.32 0.10 0.16 0.26 0.04
C1 80 7.193 0.583 1.140 13.324 20.780 2.017 4.567 12.420 3.575 1.001 1.457 2.246 3.287 1.009 0.867 1.077 0.208 0.494 2.303 2.416 2.427 0.395 1.249 1.681 0.746 0.317 0.030 0.276 0.208 0.039 0.030 0.019 0.037 0.769 0.251 0.427 0.091 0.052 1.003 0.244 0.597 0.438 0.456 0.352 1.201 0.194 0.045 0.101 0.133 1.140 0.331 0.021 0.148 0.184 0.238 0.023 0.015 0.008 0.143 0.152 0.196 0.093 0.31 0.49 0.08 0.22 0.37 0.07
C1 81 8.182 0.555 1.134 13.153 22.201 2.177 4.863 11.548 3.065 0.537 1.672 2.555 3.203 0.995 1.117 1.139 0.313 0.564 2.573 2.014 2.248 0.491 0.870 1.318 0.829 0.138 0.017 0.297 0.314 0.035 0.043 0.024 0.024 0.850 0.349 0.466 0.108 0.070 0.911 0.248 0.588 0.380 0.419 0.346 0.990 0.187 0.038 0.083 0.071 0.918 0.580 0.033 0.115 0.144 0.192 0.030 0.015 0.006 0.180 0.092 0.176 0.093 0.33 0.28 0.13 0.15 0.19 0.03
C1 84 0.907 0.986 1.648 26.094 18.449 0.373 4.949 5.053 4.201 0.898 0.243 2.580 3.042 1.759 1.544 1.194 0.270 0.382 4.757 4.416 1.641 0.568 0.394 1.330 0.370 0.057 0.011 0.211 0.154 0.055 0.038 0.078 0.005 0.426 0.292 0.542 0.102 0.050 1.364 0.437 0.581 0.497 0.751 0.806 0.873 0.291 0.019 0.113 0.314 0.850 0.759 0.021 0.194 0.300 0.296 0.037 0.010 0.004 0.146 0.118 0.091 0.152 0.15 0.34 0.19 0.08 0.13 0.02
C1 85 6.461 0.775 2.009 21.569 19.928 1.457 4.072 7.434 3.248 0.922 1.197 2.075 2.009 0.607 1.113 0.997 0.448 0.642 3.689 4.745 2.121 0.451 0.786 0.946 0.640 0.096 0.027 0.194 0.204 0.079 0.037 0.038 0.027 0.551 0.292 0.241 0.042 0.039 0.436 0.134 0.296 0.278 0.363 0.544 1.387 0.229 0.038 0.085 0.370 0.589 1.201 0.034 0.086 0.190 0.159 0.041 0.014 0.003 0.077 0.063 0.119 0.118 0.33 0.21 0.26 0.05 0.07 0.01
C1 86 4.968 0.526 3.055 14.909 25.057 1.191 5.933 6.475 2.068 1.190 0.888 2.565 3.327 1.532 1.336 1.202 0.305 0.447 2.388 5.107 1.741 0.462 0.385 0.799 0.622 0.056 0.015 0.234 0.354 0.098 0.037 0.042 0.023 0.505 0.268 0.426 0.085 0.055 1.023 0.292 0.505 0.410 0.550 0.809 1.036 0.346 0.039 0.119 0.518 0.615 1.004 0.029 0.128 0.138 0.225 0.035 0.014 0.006 0.139 0.114 0.115 0.070 0.36 0.26 0.25 0.05 0.11 0.03
C1 87 6.527 0.832 2.804 18.584 20.909 1.348 4.699 6.278 3.618 1.806 1.170 1.647 1.762 0.648 1.089 0.818 0.451 0.695 3.103 6.518 1.740 0.575 0.520 1.147 0.683 0.063 0.019 0.179 0.212 0.071 0.038 0.041 0.017 0.603 0.267 0.268 0.063 0.049 0.472 0.168 0.324 0.298 0.407 0.528 1.231 0.255 0.037 0.094 0.706 0.634 1.005 0.031 0.095 0.204 0.199 0.033 0.016 0.003 0.064 0.076 0.117 0.103 0.29 0.27 0.36 0.04 0.07 0.01
C1 88 1.102 1.002 2.417 24.358 21.616 0.262 5.506 7.211 4.460 1.537 0.256 2.238 2.008 0.718 1.551 0.962 0.205 0.271 4.013 4.486 1.965 0.842 0.111 1.105 0.197 0.009 0.001 0.094 0.252 0.093 0.034 0.022 0.003 0.181 0.196 0.313 0.076 0.041 0.546 0.167 0.276 0.357 0.528 0.562 1.338 0.363 0.005 0.171 0.215 0.527 0.961 0.025 0.093 0.252 0.227 0.050 0.017 0.009 0.086 0.102 0.044 0.125 0.28 0.43 0.37 0.04 0.09 0.02
C1 90 8.447 1.113 2.044 12.314 27.084 2.106 5.247 5.592 2.027 1.036 1.481 2.701 3.306 2.042 1.378 1.036 0.219 0.354 1.929 1.795 1.284 0.652 0.463 0.892 0.655 0.060 0.024 0.309 0.324 0.143 0.044 0.161 0.026 0.553 0.235 0.691 0.096 0.058 1.551 0.570 0.687 0.428 0.598 1.022 0.700 0.329 0.080 0.145 0.116 0.731 0.545 0.016 0.202 0.254 0.261 0.061 0.007 0.003 0.151 0.144 0.175 0.151 0.19 0.38 0.34 0.05 0.15 0.03
C1 91 5.925 0.522 2.890 18.784 18.292 1.436 6.227 7.093 2.794 0.840 1.177 2.982 3.239 1.405 1.690 1.159 0.468 0.658 3.590 1.847 1.954 0.853 0.290 0.856 0.705 0.054 0.010 0.151 0.410 0.146 0.143 0.058 0.022 0.559 0.208 0.695 0.189 0.113 1.076 0.360 0.645 0.430 0.563 0.804 0.636 0.694 0.025 0.186 0.078 0.728 0.782 0.175 0.177 0.146 0.246 0.080 0.034 0.030 0.052 0.234 0.093 0.053 0.27 0.27 0.56 0.02 0.09 0.02

10
Table S7.2: Overview of relative cells abundance per subcommunity (%) in samples taken from reactor C2 (G1 to G68). Time: time points of sampling (day).
Reacto r Time G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 G13 G14 G15 G16 G17 G18 G19 G20 G21 G22 G23 G24 G25 G26 G27 G28 G29 G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G40 G41 G42 G43 G44 G45 G46 G47 G48 G49 G50 G51 G52 G53 G54 G55 G56 G57 G58 G59 G60 G61 G62 G63 G64 G65 G66 G67 G68
C2 0.25 0.014 0.015 0.070 0.030 0.591 0.026 0.095 0.164 0.016 0.011 0.008 0.139 1.204 1.506 0.042 0.062 0.185 0.576 0.012 0.011 0.429 0.011 0.007 0.037 0.061 0.003 0.002 0.091 0.122 0.181 0.100 0.037 0.005 0.399 0.289 45.210 17.429 10.091 5.028 8.921 1.992 0.039 0.022 0.758 0.121 0.049 0.003 0.059 0.004 0.200 0.013 0.047 0.449 0.008 0.007 1.281 0.134 0.205 0.018 0.860 0.020 0.002 0.08 0.00 0.01 0.25 0.12 0.00
C2 1 0.072 0.007 0.084 0.281 0.194 2.186 0.735 3.356 0.461 0.001 0.389 1.220 5.141 7.744 3.013 3.153 0.481 1.132 0.170 0.009 1.930 1.008 0.023 1.034 0.379 0.058 0.031 1.695 0.337 0.034 0.243 0.019 0.005 2.198 0.874 21.699 4.967 2.190 7.863 7.342 2.674 2.961 1.699 0.746 0.371 0.904 0.309 0.040 0.003 1.660 0.137 0.068 0.862 0.001 0.390 0.165 0.593 0.198 0.184 0.598 0.513 0.001 0.21 0.00 0.36 0.28 0.28 0.04
C2 2 0.141 0.008 1.975 1.988 0.699 4.601 0.873 2.003 1.755 0.066 0.279 0.769 2.517 2.782 11.712 6.300 0.362 1.096 4.242 0.383 0.573 9.739 0.018 2.958 0.141 0.932 0.441 1.092 0.234 0.264 0.158 0.011 0.004 1.340 0.549 6.114 2.141 0.996 4.111 1.633 1.682 5.455 4.081 0.266 0.232 0.430 0.636 1.065 0.004 2.487 1.051 0.093 0.220 0.014 1.760 0.090 0.321 0.090 0.252 0.195 0.520 0.004 0.19 0.03 0.19 0.23 0.34 0.08
C2 3 0.232 0.038 5.109 2.367 0.546 5.638 0.996 2.297 0.952 0.200 0.406 0.999 2.938 3.875 12.473 6.039 0.489 1.725 3.451 1.184 0.179 5.893 0.068 1.843 0.233 0.673 0.391 1.455 0.272 0.789 0.158 0.050 0.116 1.549 0.694 1.909 0.648 0.400 3.013 0.646 2.019 7.439 5.423 0.702 0.538 0.531 0.678 1.699 0.017 2.912 1.715 0.073 0.115 0.058 1.026 0.067 0.087 0.022 0.226 0.146 0.810 0.027 0.15 0.06 0.16 0.12 0.19 0.05
C2 5 0.694 0.155 3.120 5.224 1.062 0.827 0.945 6.811 2.314 0.431 0.205 5.364 2.850 0.990 15.487 2.825 0.166 0.729 1.288 1.397 1.449 1.322 0.476 6.743 0.417 1.071 0.366 0.373 1.116 0.181 0.618 0.041 0.944 1.960 0.321 0.610 0.193 0.101 0.731 0.315 0.504 0.849 3.604 7.788 5.659 0.706 0.062 0.375 0.515 2.609 0.721 0.101 0.361 0.230 1.091 0.088 0.024 0.015 0.117 0.267 0.087 0.078 1.03 0.14 0.37 0.17 0.16 0.05
C2 7 0.220 0.225 5.481 1.691 0.253 0.457 0.526 7.308 0.562 0.315 0.127 6.515 1.361 0.629 2.734 1.186 0.055 0.251 0.451 1.649 1.098 0.532 0.401 1.944 0.148 0.605 0.217 0.147 17.342 0.152 11.375 0.034 0.082 0.643 0.111 0.581 0.225 0.109 0.323 0.222 0.222 0.390 0.520 15.231 7.363 1.974 0.043 0.196 0.867 0.739 0.280 0.090 0.504 0.309 0.187 0.041 0.037 0.017 0.050 1.103 0.042 0.032 1.12 0.05 0.09 0.08 0.09 0.03
C2 8 0.588 0.329 8.396 2.052 1.189 1.144 1.286 2.888 1.759 0.389 0.831 4.315 1.682 0.796 3.049 1.318 0.407 0.888 0.689 1.420 1.140 0.670 3.413 3.803 0.863 1.941 1.098 0.914 8.261 0.272 11.913 0.148 0.215 2.083 0.555 0.601 0.171 0.110 0.440 0.352 0.611 2.241 1.301 7.283 3.982 1.556 0.297 0.286 0.566 2.067 0.346 0.284 0.149 0.332 0.177 0.131 0.039 0.010 0.353 0.117 0.265 0.147 1.51 0.20 0.12 0.49 0.58 0.20
C2 9 0.717 2.721 27.708 6.979 1.348 0.523 0.766 0.939 1.830 2.355 0.119 0.334 1.122 0.718 3.043 1.785 0.198 0.764 1.687 8.388 0.153 1.063 0.451 6.440 0.569 1.560 0.539 0.463 0.297 0.211 0.283 0.203 0.217 2.209 0.311 0.354 0.093 0.065 0.660 0.167 0.523 1.120 0.834 0.311 0.231 0.226 0.079 0.162 7.791 2.431 0.198 0.115 0.047 3.690 0.197 0.077 0.030 0.008 0.120 0.036 0.151 0.179 0.08 0.34 0.10 0.27 0.24 0.06
C2 10 1.481 1.008 29.446 10.063 0.858 0.519 0.488 0.613 4.981 7.122 0.182 0.282 0.741 0.426 1.044 0.602 0.203 0.699 4.817 5.629 0.143 0.622 0.266 5.058 0.790 0.796 0.217 0.511 0.077 0.678 0.116 0.229 0.083 1.246 0.370 0.184 0.057 0.042 0.396 0.095 0.378 0.543 0.833 0.067 0.032 0.132 0.079 0.715 3.627 2.154 0.390 0.064 0.027 4.278 0.196 0.066 0.017 0.003 0.098 0.041 0.199 0.612 0.03 1.82 0.09 0.18 0.13 0.02
C2 11 0.805 0.317 30.915 9.573 0.690 0.848 1.406 0.611 5.211 5.388 0.516 0.176 0.534 0.295 2.563 0.726 0.218 0.870 5.142 6.540 0.141 0.759 0.104 4.729 0.608 0.564 0.205 0.316 0.067 0.242 0.080 0.065 0.048 1.128 0.361 0.172 0.041 0.019 0.353 0.055 0.523 0.676 1.540 0.046 0.027 0.266 0.068 1.160 3.780 2.223 0.613 0.023 0.014 2.097 0.422 0.039 0.006 0.005 0.084 0.016 0.142 0.216 0.02 0.91 0.12 0.75 0.77 0.05
C2 12 0.534 0.279 28.427 8.301 0.461 0.897 1.467 0.667 4.807 4.204 0.904 0.247 0.574 0.244 8.097 1.110 0.164 0.867 3.613 5.528 0.086 1.507 0.157 5.192 0.430 0.823 0.320 0.306 0.159 0.152 0.184 0.050 0.119 1.206 0.323 0.116 0.022 0.017 0.353 0.047 0.563 1.062 3.110 0.036 0.027 0.291 0.052 0.846 3.191 2.582 0.529 0.018 0.007 1.449 1.039 0.026 0.004 0.002 0.062 0.013 0.108 0.129 0.04 0.55 0.14 0.51 0.60 0.05
C2 14 0.250 0.229 17.786 6.288 0.233 0.169 1.094 0.682 5.160 2.253 0.056 0.518 0.746 0.370 26.449 1.495 0.101 0.287 2.182 5.619 0.082 3.522 0.069 4.976 0.191 0.076 0.027 0.150 0.133 0.106 0.202 0.034 0.073 0.445 0.132 0.111 0.018 0.012 0.482 0.057 0.346 1.151 4.956 0.066 0.040 0.229 0.021 0.649 2.323 1.706 0.495 0.029 0.014 0.746 2.363 0.033 0.002 0.004 0.121 0.008 0.055 0.062 0.04 0.21 0.10 0.58 0.70 0.08
C2 15 0.269 0.263 14.403 5.106 0.089 0.107 0.533 0.662 4.469 2.796 0.043 0.254 0.709 0.351 30.828 1.641 0.049 0.239 2.639 5.041 0.062 3.886 0.023 6.456 0.179 0.055 0.018 0.086 0.096 0.049 0.096 0.020 0.038 0.353 0.088 0.259 0.049 0.037 0.610 0.108 0.473 1.449 5.542 0.071 0.031 0.176 0.015 0.223 2.539 1.803 0.255 0.023 0.012 1.027 2.394 0.014 0.004 0.000 0.056 0.014 0.027 0.069 0.02 0.35 0.04 0.13 0.15 0.03
C2 17 0.098 0.251 13.678 4.966 0.050 0.207 0.337 2.657 2.526 2.026 0.041 0.264 1.039 0.432 35.591 3.359 0.049 0.219 1.666 4.718 0.069 2.339 0.023 4.148 0.073 0.166 0.055 0.114 0.063 0.061 0.061 0.026 0.017 0.234 0.087 0.295 0.100 0.073 0.682 0.137 0.436 1.899 7.216 0.089 0.039 0.106 0.028 0.274 1.607 1.151 0.370 0.014 0.023 0.828 2.368 0.008 0.003 0.001 0.022 0.024 0.045 0.045 0.02 0.18 0.05 0.08 0.07 0.01
C2 18 0.233 0.403 7.028 4.297 0.033 0.453 0.327 1.958 2.764 2.878 0.041 0.174 1.032 0.433 33.552 3.930 0.061 0.375 2.832 7.417 0.088 4.098 0.033 5.269 0.154 0.222 0.118 0.205 0.061 0.054 0.051 0.046 0.037 0.362 0.134 0.395 0.124 0.088 0.715 0.144 0.605 2.721 6.203 0.096 0.037 0.129 0.046 0.129 2.323 1.510 0.385 0.014 0.018 0.627 1.797 0.008 0.006 0.001 0.028 0.020 0.097 0.069 0.02 0.31 0.04 0.07 0.05 0.01
C2 19 0.243 0.412 6.458 3.735 0.037 0.493 0.167 3.043 2.864 2.290 0.046 0.367 1.171 0.486 36.613 4.722 0.052 0.421 2.197 4.484 0.149 3.235 0.019 5.102 0.174 0.437 0.184 0.201 0.084 0.071 0.036 0.115 0.033 0.401 0.149 0.444 0.130 0.104 0.665 0.143 0.589 2.690 7.172 0.119 0.038 0.121 0.043 0.166 1.864 1.432 0.216 0.013 0.033 0.608 1.989 0.008 0.003 0.001 0.020 0.026 0.097 0.100 0.01 0.32 0.07 0.03 0.04 0.01
C2 21 0.131 0.383 4.184 5.979 0.052 0.732 0.136 7.926 2.848 1.364 0.054 6.336 2.261 0.608 21.391 10.318 0.056 0.312 3.461 7.274 0.090 2.700 0.062 2.402 0.137 0.394 0.194 0.223 0.295 0.301 0.030 0.160 0.276 0.349 0.127 0.343 0.122 0.080 0.647 0.112 0.412 4.244 3.526 0.103 0.056 0.662 0.053 0.160 0.936 0.959 2.351 0.015 0.027 0.175 0.541 0.010 0.005 0.000 0.015 0.019 0.150 0.058 0.02 0.18 0.45 0.00 0.02 0.00
C2 22 0.118 0.509 4.196 9.587 0.075 0.282 0.113 11.278 2.236 1.179 0.042 14.540 2.276 0.366 15.817 6.729 0.048 0.206 2.872 6.879 0.111 1.477 0.016 1.369 0.087 0.121 0.059 0.178 1.412 0.353 0.030 0.394 0.049 0.235 0.098 0.184 0.045 0.039 0.354 0.056 0.212 2.167 1.756 0.124 0.064 3.109 0.038 0.125 1.272 0.427 1.991 0.014 0.017 0.167 0.310 0.014 0.004 0.000 0.017 0.020 0.077 0.062 0.02 0.11 1.83 0.02 0.02 0.00
C2 23 0.313 0.599 4.579 11.628 0.095 0.415 0.144 9.954 2.453 1.490 0.231 14.970 1.573 0.277 11.425 5.849 0.051 0.202 3.312 6.740 0.088 1.194 0.022 2.911 0.891 0.108 0.051 0.145 1.298 0.434 0.027 0.625 0.102 0.637 0.085 0.136 0.031 0.032 0.282 0.055 0.212 2.081 1.543 0.087 0.048 2.768 0.030 0.158 1.580 0.920 2.065 0.014 0.008 0.209 0.214 0.025 0.004 0.000 0.027 0.005 0.077 0.074 0.02 0.15 2.17 0.02 0.03 0.00
C2 24 0.252 0.804 3.110 15.523 0.164 0.387 0.177 5.438 4.651 2.070 0.073 1.197 1.979 0.570 16.865 8.334 0.088 0.348 6.830 7.299 0.146 3.121 0.051 3.200 0.178 0.126 0.070 0.219 0.165 0.427 0.035 0.684 0.105 0.431 0.155 0.284 0.048 0.021 0.585 0.093 0.396 3.201 2.304 0.165 0.065 0.978 0.048 0.134 0.995 1.014 2.224 0.021 0.014 0.253 0.449 0.024 0.007 0.000 0.041 0.009 0.132 0.103 0.01 0.25 0.82 0.01 0.02 0.00
C2 25 0.313 0.715 2.175 15.016 0.213 0.489 0.231 5.842 4.650 1.558 0.090 1.755 2.695 0.861 14.495 9.673 0.106 0.514 6.807 4.528 0.204 2.989 0.075 3.683 0.584 0.110 0.066 0.253 0.125 0.404 0.027 0.631 0.068 1.048 0.196 0.333 0.052 0.037 0.905 0.134 0.678 3.312 2.284 0.197 0.091 2.070 0.042 0.265 0.522 1.575 1.483 0.009 0.027 0.225 0.576 0.021 0.003 0.000 0.039 0.009 0.186 0.061 0.01 0.30 1.29 0.02 0.03 0.01
C2 28 0.456 0.658 2.661 8.403 0.311 0.563 0.242 2.470 3.028 0.808 0.101 3.876 2.854 1.351 12.563 7.156 0.080 0.558 3.721 4.791 0.229 4.318 0.193 3.592 0.489 0.087 0.077 0.283 0.509 0.825 0.046 1.387 0.054 1.242 0.199 0.407 0.070 0.037 1.460 0.253 0.637 3.270 3.571 0.105 0.023 8.356 0.047 0.163 0.661 1.709 3.761 0.018 0.034 0.226 1.040 0.015 0.003 0.002 0.018 0.014 0.189 0.048 0.01 0.14 3.50 0.01 0.01 0.00
C2 29 0.345 0.482 8.340 9.983 0.207 0.536 0.164 1.449 2.012 0.949 0.137 3.633 1.944 0.898 9.552 5.496 0.032 0.358 3.048 8.553 0.219 3.418 0.217 2.445 0.171 0.063 0.061 0.253 0.603 4.933 0.075 4.106 0.031 0.714 0.117 0.250 0.045 0.027 0.848 0.169 0.330 2.083 2.412 0.070 0.019 6.620 0.057 0.384 0.616 0.819 5.016 0.036 0.022 0.144 0.742 0.022 0.005 0.001 0.015 0.010 0.155 0.020 0.01 0.11 3.39 0.00 0.01 0.00
C2 30 0.375 0.407 9.428 10.355 0.186 0.502 0.184 1.103 2.266 1.061 0.121 2.000 1.539 0.807 8.847 3.646 0.021 0.203 3.801 9.823 0.168 2.797 0.243 2.792 0.151 0.197 0.107 0.129 0.348 5.376 0.046 3.965 0.037 0.492 0.065 0.292 0.049 0.028 1.022 0.196 0.415 1.752 2.292 0.051 0.018 6.302 0.047 0.515 0.736 0.990 7.667 0.030 0.024 0.136 0.655 0.056 0.005 0.002 0.004 0.009 0.096 0.021 0.01 0.13 2.85 0.00 0.01 0.00
C2 31 0.388 0.264 8.193 10.465 0.083 0.505 0.109 1.290 2.882 1.064 0.145 1.410 1.810 1.083 7.941 3.422 0.030 0.292 4.202 9.138 0.152 2.806 0.259 3.294 0.191 0.065 0.050 0.117 0.193 5.705 0.032 3.759 0.018 0.536 0.080 0.542 0.072 0.038 1.450 0.311 0.814 2.267 2.819 0.057 0.023 4.922 0.069 0.638 0.529 1.574 7.816 0.010 0.022 0.116 0.751 0.062 0.004 0.001 0.008 0.016 0.091 0.019 0.01 0.12 2.83 0.00 0.01 0.00
C2 35 0.474 0.661 11.333 12.264 0.436 0.452 0.269 0.639 2.680 1.574 0.101 2.117 0.468 0.298 6.328 1.508 0.048 0.175 3.642 7.842 0.123 2.102 0.215 3.973 0.154 0.055 0.066 0.161 0.247 5.843 0.072 4.906 0.020 0.564 0.083 0.167 0.032 0.021 0.357 0.067 0.245 0.833 1.293 0.037 0.017 6.978 0.056 0.761 1.081 0.991 7.741 0.029 0.012 0.264 0.460 0.363 0.029 0.004 0.027 0.012 0.109 0.088 0.04 0.36 5.55 0.03 0.04 0.01
C2 36 0.460 0.479 11.070 12.308 0.286 0.415 0.141 1.960 4.900 2.005 0.114 6.480 1.261 0.620 6.857 2.414 0.024 0.151 3.653 6.327 0.526 2.053 0.266 5.161 0.133 0.060 0.031 0.097 2.892 5.632 0.080 2.265 0.019 0.787 0.056 0.211 0.049 0.031 0.536 0.117 0.250 0.934 1.868 0.141 0.068 2.994 0.039 2.192 1.115 1.249 1.195 0.038 0.041 0.253 0.675 0.068 0.008 0.002 0.011 0.033 0.072 0.088 1.13 0.43 2.15 0.01 0.01 0.00
C2 37 0.453 0.678 10.666 19.655 0.517 0.349 1.507 1.249 5.175 2.254 0.084 3.654 0.970 0.466 7.262 2.023 0.033 0.162 3.725 6.027 0.459 1.964 0.174 5.923 0.119 0.075 0.026 0.098 1.235 3.280 0.083 0.881 0.024 0.755 0.065 0.159 0.038 0.017 0.420 0.087 0.222 0.911 2.001 0.191 0.155 2.038 0.046 2.547 1.417 1.170 2.136 0.047 0.052 0.373 0.762 0.053 0.008 0.002 0.008 0.031 0.076 0.192 0.91 0.50 1.34 0.01 0.01 0.00
C2 38 1.309 0.529 7.159 28.773 0.641 0.390 0.495 0.827 7.562 2.129 0.207 1.359 0.666 0.341 6.074 1.210 0.041 0.240 5.629 6.294 0.202 1.273 0.296 5.263 0.223 0.253 0.028 0.109 0.603 2.742 0.121 0.631 0.236 0.768 0.085 0.195 0.043 0.032 0.404 0.098 0.346 0.786 2.394 0.082 0.064 1.684 0.062 1.848 0.849 1.544 1.760 0.085 0.022 0.367 0.679 0.047 0.009 0.002 0.010 0.011 0.066 0.316 0.16 0.53 0.78 0.00 0.01 0.00
C2 39 0.924 0.795 4.189 28.881 0.629 0.689 0.333 0.354 10.767 2.001 0.245 0.692 0.445 0.285 4.198 0.521 0.064 0.450 6.527 8.574 0.082 1.698 0.217 7.795 0.564 0.234 0.065 0.168 0.199 1.387 0.167 0.597 0.091 1.033 0.145 0.211 0.028 0.017 0.499 0.070 0.631 0.853 1.503 0.041 0.019 1.035 0.135 0.158 0.936 2.627 2.222 0.115 0.007 0.422 0.722 0.058 0.017 0.002 0.020 0.014 0.098 0.267 0.02 0.80 0.39 0.02 0.03 0.00
C2 43 0.556 1.248 7.073 24.726 0.516 0.285 0.210 0.489 9.573 1.984 0.077 0.706 0.508 0.344 3.113 0.391 0.044 0.732 6.088 9.724 0.093 1.169 0.064 6.070 0.614 0.032 0.024 0.115 0.152 4.423 0.104 2.163 0.011 1.065 0.154 0.355 0.050 0.029 0.704 0.111 1.158 0.790 0.994 0.068 0.026 0.998 0.027 0.413 1.292 3.154 1.753 0.011 0.004 1.117 0.390 0.050 0.008 0.002 0.005 0.002 0.051 0.662 0.04 0.68 0.40 0.01 0.01 0.00
C2 44 0.464 1.377 6.730 24.365 0.575 0.242 0.247 0.326 9.511 1.647 0.085 0.495 0.484 0.373 2.564 0.377 0.056 0.799 6.815 10.902 0.061 1.200 0.053 5.539 0.645 0.039 0.026 0.114 0.134 4.142 0.101 2.218 0.010 1.028 0.159 0.380 0.057 0.032 0.805 0.126 1.248 0.938 1.023 0.042 0.025 0.824 0.026 0.357 1.334 3.577 1.830 0.010 0.005 1.311 0.412 0.067 0.005 0.001 0.011 0.004 0.051 0.616 0.05 0.57 0.33 0.01 0.01 0.00
C2 45 0.460 0.394 7.116 24.779 0.330 0.397 1.783 0.822 9.932 1.300 0.150 1.053 0.817 0.528 3.786 0.735 0.195 0.929 6.315 6.952 0.103 2.339 0.077 4.503 0.335 0.038 0.030 0.530 0.252 0.773 0.073 0.247 0.009 1.095 0.349 0.801 0.208 0.153 1.039 0.262 1.388 1.084 1.698 0.097 0.055 1.230 0.107 1.440 1.251 3.482 3.648 0.042 0.018 0.357 0.700 0.064 0.005 0.008 0.041 0.018 0.153 0.050 0.10 0.36 0.54 0.03 0.05 0.00
C2 46 0.268 0.752 5.766 27.446 0.613 0.185 0.347 0.368 9.909 1.578 0.058 0.619 0.728 0.589 3.403 0.429 0.054 0.775 7.465 8.089 0.085 1.546 0.040 6.016 0.520 0.029 0.024 0.130 0.165 2.569 0.047 0.394 0.014 0.929 0.156 0.604 0.105 0.077 1.128 0.229 1.496 1.162 1.366 0.065 0.029 0.965 0.042 1.701 0.737 3.938 1.963 0.024 0.013 0.273 0.494 0.035 0.006 0.008 0.012 0.013 0.060 0.242 0.06 0.60 0.42 0.01 0.02 0.00
C2 49 0.945 0.171 0.336 2.416 1.057 0.199 0.160 18.089 1.277 0.795 0.070 6.661 6.234 4.484 1.549 1.909 0.069 0.192 1.128 0.490 12.287 0.601 0.499 1.172 0.218 0.183 0.057 0.085 2.507 0.066 0.151 0.029 0.252 0.259 0.106 0.506 0.144 0.071 0.826 0.306 0.382 0.397 0.470 17.103 2.962 0.812 0.031 0.043 0.089 0.507 0.304 0.141 1.530 0.158 0.183 0.258 0.235 0.063 0.041 0.544 0.038 0.036 4.42 0.11 0.37 0.04 0.09 0.09
C2 50 0.391 0.131 6.717 2.968 0.305 0.162 0.197 13.012 1.995 0.714 0.057 6.691 5.083 1.953 5.546 4.355 0.117 0.285 1.772 1.153 3.123 1.331 0.018 2.532 0.172 0.036 0.028 0.117 9.509 0.074 1.403 0.030 0.025 0.257 0.191 0.368 0.100 0.066 0.772 0.248 0.337 1.728 1.737 10.212 3.979 2.590 0.027 0.123 0.142 1.189 0.570 0.028 0.474 0.323 0.878 0.042 0.072 0.042 0.062 0.278 0.041 0.035 0.45 0.12 0.39 0.03 0.06 0.03
C2 51 0.578 0.197 7.303 3.482 0.258 0.164 0.507 16.032 3.134 0.795 0.105 4.112 5.315 1.688 1.815 4.195 0.081 0.222 1.239 2.177 5.879 2.980 0.066 1.034 0.219 0.068 0.016 0.113 5.359 0.042 0.319 0.010 0.092 0.354 0.113 0.440 0.131 0.061 0.812 0.262 0.319 1.364 2.102 7.529 4.359 1.558 0.019 0.135 0.233 1.638 3.207 0.019 0.442 0.247 2.512 0.027 0.036 0.029 0.028 0.505 0.087 0.041 1.25 0.13 0.35 0.02 0.03 0.02
C2 52 2.297 0.207 4.133 2.776 0.128 0.308 0.192 23.077 4.076 0.862 0.264 4.080 4.809 1.212 6.151 4.660 0.063 0.202 3.113 1.419 2.922 3.266 0.297 2.951 0.526 0.295 0.019 0.117 4.502 0.067 0.726 0.015 0.782 0.415 0.107 0.266 0.076 0.041 0.537 0.148 0.253 1.207 1.879 5.889 2.263 1.763 0.022 0.138 0.300 1.174 0.350 0.018 0.188 0.391 0.570 0.035 0.028 0.016 0.035 0.184 0.114 0.098 0.35 0.19 0.38 0.01 0.03 0.02
C2 53 0.353 0.017 1.175 1.339 0.176 0.090 0.119 25.537 0.818 0.223 0.039 6.877 8.649 2.287 3.716 4.625 0.050 0.077 0.713 0.366 11.201 0.817 0.045 1.494 0.084 0.050 0.031 0.069 1.207 0.014 0.100 0.011 0.854 0.101 0.076 0.120 0.037 0.019 0.388 0.111 0.096 1.495 1.266 11.486 6.248 1.721 0.022 0.040 0.014 0.300 0.612 0.022 0.441 0.033 0.276 0.034 0.045 0.016 0.029 0.207 0.027 0.012 0.61 0.02 0.78 0.01 0.03 0.03
C2 56 0.264 0.033 7.980 1.696 0.095 0.229 0.061 20.727 0.429 0.158 0.052 2.008 5.480 1.305 4.996 5.196 0.018 0.061 0.446 0.945 6.248 0.412 0.015 0.603 0.024 0.057 0.036 0.048 0.383 0.093 0.044 0.013 0.044 0.045 0.031 0.104 0.035 0.018 0.163 0.062 0.041 1.561 0.916 22.234 11.240 0.571 0.060 0.336 0.068 0.101 0.244 0.011 0.309 0.180 0.131 0.012 0.072 0.011 0.020 0.193 0.040 0.017 0.56 0.01 0.34 0.01 0.03 0.02
C2 57 0.564 0.020 4.549 1.151 0.091 0.126 0.057 23.092 0.327 0.107 0.112 2.212 5.619 0.849 4.159 3.257 0.028 0.064 0.340 0.663 4.725 0.308 0.033 0.432 0.022 0.152 0.054 0.044 0.371 0.055 0.046 0.011 0.086 0.062 0.049 0.139 0.036 0.024 0.132 0.071 0.072 1.145 1.709 25.721 13.635 0.536 0.023 0.146 0.048 0.096 0.212 0.013 0.354 0.113 0.128 0.014 0.061 0.014 0.025 0.181 0.012 0.039 1.09 0.02 0.26 0.03 0.04 0.03
C2 58 2.311 0.017 2.833 1.245 0.083 0.312 0.030 19.054 0.410 0.110 0.315 1.558 7.300 2.050 3.127 3.739 0.022 0.060 0.383 0.584 6.640 0.368 0.117 0.459 0.093 0.414 0.095 0.067 0.269 0.030 0.032 0.005 0.465 0.074 0.034 0.259 0.062 0.034 0.277 0.143 0.121 1.406 0.788 25.535 12.917 0.263 0.041 0.098 0.031 0.179 0.226 0.009 0.958 0.069 0.098 0.021 0.089 0.015 0.022 0.596 0.029 0.119 0.55 0.04 0.19 0.04 0.04 0.02
C2 59 0.452 0.008 1.353 0.996 0.095 0.441 0.082 15.427 0.625 0.123 0.347 2.060 7.299 1.742 1.633 1.520 0.050 0.147 0.670 0.365 4.892 0.819 0.180 0.514 0.062 0.390 0.202 0.115 0.430 0.014 0.062 0.006 0.992 0.131 0.062 0.607 0.175 0.083 0.352 0.388 0.247 0.776 0.856 32.156 14.641 0.371 0.082 0.078 0.006 0.455 0.430 0.014 1.050 0.010 0.225 0.017 0.097 0.020 0.045 0.889 0.050 0.041 1.14 0.02 0.20 0.08 0.07 0.02
C2 60 0.310 0.015 4.106 2.090 0.108 1.297 0.381 11.242 1.238 0.277 1.053 2.089 6.170 3.001 1.589 2.278 0.105 0.305 1.010 0.887 5.358 1.673 0.526 0.368 0.091 1.984 0.752 0.322 0.532 0.010 0.113 0.005 0.603 0.367 0.156 1.081 0.240 0.120 0.575 0.718 0.778 0.953 0.712 24.368 11.303 0.426 0.242 0.158 0.006 0.671 0.928 0.017 0.894 0.023 0.957 0.025 0.125 0.015 0.097 0.956 0.099 0.024 0.57 0.03 0.19 0.09 0.13 0.06
C2 65 0.139 0.135 2.899 4.054 0.068 0.926 0.440 4.500 10.008 0.690 0.321 2.755 1.543 0.471 1.583 0.893 0.195 0.400 7.528 0.816 1.181 3.584 2.274 3.533 0.796 3.370 1.550 0.573 2.939 0.027 3.810 0.001 0.026 0.992 0.362 0.437 0.136 0.061 0.260 0.386 0.366 0.355 0.629 16.130 6.955 0.601 0.252 0.531 0.059 2.142 0.405 0.055 0.243 0.273 0.399 0.010 0.059 0.008 0.233 0.194 0.138 0.127 2.17 0.20 0.20 0.22 0.27 0.13
C2 66 0.189 0.159 2.142 6.456 0.065 1.045 0.390 2.718 9.450 0.698 0.546 2.492 1.107 0.462 1.404 0.985 0.330 0.514 9.280 1.602 0.714 5.675 2.989 3.725 0.543 1.758 1.185 0.706 3.459 0.036 5.636 0.004 0.040 1.217 0.432 0.391 0.107 0.059 0.251 0.412 0.412 0.440 0.813 11.865 5.077 0.491 0.264 0.493 0.133 2.297 0.482 0.106 0.415 0.555 0.501 0.014 0.044 0.010 0.316 0.198 0.168 0.161 1.73 0.24 0.24 0.45 0.53 0.18
C2 67 0.263 0.559 2.444 4.016 0.075 0.872 0.820 2.057 10.977 0.681 0.226 3.337 1.025 0.394 1.244 0.896 0.162 0.414 10.193 1.415 0.869 4.906 1.511 4.613 0.626 2.519 1.587 0.469 3.705 0.057 9.244 0.007 0.026 0.958 0.308 0.234 0.046 0.029 0.234 0.116 0.367 0.420 0.938 9.777 4.646 0.576 0.177 0.481 0.320 2.507 0.347 0.509 0.194 1.153 0.481 0.012 0.031 0.009 0.142 0.074 0.119 0.365 1.45 0.19 0.20 0.14 0.17 0.07
C2 69 0.530 0.247 2.349 6.980 0.120 1.462 0.449 1.059 8.202 2.696 0.696 2.024 0.687 0.291 2.043 1.024 0.360 0.595 11.574 5.010 0.399 10.252 4.429 4.891 0.597 1.114 1.149 0.679 2.206 0.048 7.560 0.004 0.021 1.102 0.483 0.273 0.059 0.036 0.276 0.152 0.548 0.619 1.072 2.699 1.180 0.415 0.300 0.308 0.388 2.454 0.492 0.931 0.068 1.014 0.813 0.011 0.043 0.010 0.264 0.057 0.193 0.268 0.59 0.41 0.17 0.13 0.32 0.11
C2 70 0.208 3.477 2.380 10.001 0.107 0.823 0.669 1.230 10.021 1.684 0.168 1.546 0.711 0.447 1.060 0.798 0.157 0.332 12.446 5.074 0.981 7.420 3.779 3.512 0.415 2.935 0.937 0.327 2.719 0.071 8.125 0.053 0.041 0.585 0.207 0.253 0.049 0.038 0.266 0.119 0.330 0.343 0.688 1.149 0.480 0.629 0.130 0.147 1.997 1.839 0.483 0.340 0.092 2.107 0.634 0.033 0.028 0.003 0.135 0.082 0.122 0.600 0.32 0.56 0.17 0.13 0.20 0.06
C2 71 0.292 1.825 3.300 12.506 0.121 0.776 0.234 0.844 6.107 1.803 0.308 0.788 0.611 0.390 1.602 0.825 0.157 0.370 16.223 9.968 0.613 9.693 3.052 4.231 0.392 1.157 0.586 0.371 0.828 0.077 1.285 0.023 0.059 0.728 0.223 0.234 0.054 0.040 0.246 0.126 0.327 0.552 0.842 1.221 0.709 0.653 0.120 0.143 3.207 1.669 0.846 0.057 0.060 3.749 0.663 0.034 0.018 0.003 0.111 0.050 0.132 0.401 0.49 0.37 0.10 0.18 0.20 0.04
C2 72 0.281 1.738 2.026 20.483 0.273 1.046 0.207 0.564 9.946 2.503 0.342 0.873 0.450 0.235 0.753 0.871 0.199 0.604 10.653 8.886 0.196 8.273 1.929 3.292 0.349 1.494 0.507 0.478 0.879 0.036 1.295 0.001 0.012 1.241 0.347 0.155 0.036 0.026 0.266 0.062 0.324 0.542 0.963 1.693 0.610 0.533 0.122 0.132 1.724 2.458 0.468 0.048 0.032 2.705 1.145 0.007 0.007 0.001 0.115 0.030 0.190 0.928 0.22 0.79 0.11 0.12 0.14 0.03
C2 73 0.343 4.619 2.187 10.890 0.118 1.052 0.108 0.506 12.680 1.689 0.150 1.179 0.402 0.269 0.801 0.798 0.317 0.807 11.847 5.164 0.180 8.327 0.883 4.831 0.763 1.237 0.336 0.702 0.965 0.068 1.081 0.010 0.010 1.452 0.512 0.161 0.043 0.032 0.313 0.055 0.506 0.654 0.949 3.514 1.229 0.279 0.108 0.124 2.934 3.320 0.191 0.032 0.062 3.812 0.968 0.011 0.012 0.006 0.203 0.046 0.309 0.992 0.37 0.92 0.10 0.19 0.22 0.05
C2 74 0.444 3.245 2.706 13.206 0.121 1.986 0.132 0.438 8.813 1.699 0.237 0.825 0.242 0.181 0.814 0.595 0.423 1.089 13.027 6.898 0.143 6.690 1.671 4.559 1.049 1.248 0.331 1.186 0.658 0.068 0.193 0.007 0.017 2.251 0.664 0.172 0.028 0.033 0.285 0.065 0.506 0.447 0.740 3.232 1.301 0.228 0.197 0.143 3.343 2.238 0.422 0.007 0.065 4.308 0.601 0.007 0.004 0.000 0.320 0.071 0.752 0.834 0.38 0.72 0.07 0.22 0.32 0.09
C2 77 0.385 2.465 2.727 15.925 0.124 1.027 0.142 0.655 10.178 2.104 0.168 0.655 0.452 0.329 1.317 0.727 0.417 0.883 7.237 6.741 0.193 7.810 1.427 4.306 0.575 1.675 0.575 0.966 0.694 0.073 0.149 0.003 0.037 1.267 0.632 0.319 0.081 0.052 0.328 0.215 0.355 0.682 1.206 5.605 1.883 0.337 0.138 0.236 1.402 3.007 0.423 0.009 0.321 3.026 1.407 0.011 0.016 0.003 0.276 0.221 0.337 1.454 0.29 0.84 0.09 0.08 0.23 0.09
C2 78 0.447 2.929 2.088 17.566 0.137 0.644 0.133 0.732 11.747 2.539 0.080 0.725 0.437 0.338 1.195 0.574 0.475 0.883 7.615 6.268 0.186 4.390 0.572 4.388 0.912 0.946 0.315 0.827 0.734 0.072 0.202 0.001 0.022 1.102 0.645 0.366 0.092 0.057 0.394 0.166 0.502 0.532 0.807 6.966 2.471 0.383 0.101 0.140 1.581 3.093 0.390 0.008 0.399 3.417 0.735 0.005 0.009 0.015 0.254 0.167 0.275 1.535 0.26 1.40 0.10 0.17 0.28 0.07
C2 79 0.600 2.698 1.928 16.039 0.128 0.643 0.134 0.589 11.699 2.871 0.106 0.707 0.471 0.360 1.682 0.496 0.614 1.067 7.318 5.070 0.189 5.395 0.470 4.789 1.087 1.363 0.463 0.934 0.715 0.046 0.167 0.002 0.016 1.255 0.821 0.436 0.133 0.075 0.435 0.222 0.608 0.547 0.931 7.385 2.459 0.459 0.122 0.119 0.610 3.392 0.370 0.008 0.451 2.369 0.754 0.010 0.007 0.008 0.388 0.231 0.247 1.984 0.26 1.35 0.11 0.13 0.34 0.12
C2 80 0.731 4.141 1.755 16.026 0.135 0.588 0.155 0.640 11.467 2.724 0.111 0.802 0.512 0.467 1.542 0.467 0.690 1.114 7.270 4.191 0.450 3.197 0.592 4.615 1.279 1.414 0.449 1.048 0.673 0.052 0.224 0.014 0.013 1.228 0.888 0.491 0.146 0.097 0.472 0.220 0.576 0.474 0.747 7.486 2.856 0.541 0.099 0.072 1.203 2.998 0.412 0.008 0.469 3.204 0.491 0.009 0.005 0.004 0.422 0.224 0.244 1.522 0.19 1.72 0.18 0.19 0.42 0.13
C2 81 0.778 3.016 1.750 14.488 0.123 0.584 0.141 0.687 11.692 3.195 0.121 1.345 0.496 0.422 1.605 0.532 0.721 1.187 6.330 5.076 0.238 4.125 0.867 4.534 0.982 1.444 0.493 1.012 0.794 0.045 0.398 0.002 0.005 1.315 0.931 0.580 0.180 0.122 0.470 0.309 0.612 0.516 0.946 7.621 2.636 0.385 0.091 0.078 0.791 3.350 0.492 0.012 0.646 2.495 0.723 0.006 0.009 0.001 0.445 0.331 0.254 1.746 0.18 1.47 0.17 0.25 0.48 0.13
C2 84 0.391 2.015 4.823 16.749 0.169 1.025 0.300 0.452 7.381 4.048 0.289 1.314 0.539 0.366 1.752 0.544 0.773 1.738 6.686 7.372 0.179 3.074 2.941 4.242 0.693 1.838 0.847 1.288 0.503 0.087 0.491 0.007 0.008 2.029 1.150 0.481 0.121 0.101 0.606 0.211 0.880 0.722 0.976 4.619 1.667 0.397 0.122 0.314 0.635 3.201 0.748 0.022 0.122 1.335 0.492 0.009 0.021 0.009 0.428 0.095 0.320 0.886 0.22 1.19 0.22 0.21 0.41 0.11
C2 85 0.472 3.080 5.269 17.286 0.187 1.084 0.377 0.470 5.989 3.798 0.164 0.923 0.472 0.352 1.893 0.438 0.794 1.762 7.089 8.454 0.270 3.282 2.944 4.016 0.725 2.024 0.610 1.453 0.304 0.113 0.047 0.004 0.012 2.068 1.179 0.412 0.091 0.073 0.559 0.208 0.751 0.545 0.855 3.422 1.340 0.411 0.132 0.299 1.274 2.467 0.758 0.004 0.103 2.147 0.355 0.018 0.013 0.004 0.520 0.086 0.366 0.593 0.32 1.33 0.39 0.18 0.43 0.14
C2 86 0.387 1.069 7.021 16.099 0.129 1.170 0.516 0.601 5.917 3.036 0.384 1.136 0.546 0.382 2.664 0.687 0.925 1.976 5.936 6.985 0.168 5.315 4.134 4.277 0.579 1.446 0.704 1.366 0.453 0.146 0.056 0.005 0.011 2.347 1.254 0.440 0.109 0.080 0.628 0.183 0.951 0.839 1.359 2.897 1.228 0.478 0.136 0.451 0.703 2.785 1.004 0.005 0.064 1.200 0.735 0.013 0.008 0.003 0.517 0.098 0.318 0.344 0.27 0.98 0.42 0.28 0.53 0.12
C2 87 0.381 1.466 6.003 18.191 0.123 1.097 0.443 0.535 6.996 3.141 0.217 1.228 0.606 0.442 2.279 0.571 0.891 1.946 7.329 6.054 0.252 3.753 2.591 4.708 0.653 2.027 0.790 1.480 0.340 0.079 0.043 0.008 0.011 2.177 1.266 0.518 0.115 0.092 0.745 0.253 0.895 0.774 1.115 2.593 0.993 0.442 0.144 0.226 0.828 2.835 1.314 0.006 0.099 1.356 0.461 0.017 0.013 0.002 0.487 0.074 0.418 0.492 0.28 1.12 0.39 0.21 0.47 0.11
C2 88 0.391 1.076 8.443 16.249 0.079 1.216 0.471 0.710 5.563 4.220 0.398 1.127 0.787 0.589 2.727 0.622 0.711 1.593 6.184 10.565 0.923 3.112 2.050 3.899 0.510 1.602 0.718 1.217 0.468 0.096 0.078 0.030 0.029 1.831 1.015 0.464 0.108 0.079 0.702 0.219 0.767 0.750 1.072 2.236 0.930 0.657 0.142 0.274 1.207 2.440 1.906 0.014 0.102 1.108 0.514 0.029 0.014 0.004 0.412 0.068 0.354 0.383 0.28 0.53 0.32 0.16 0.35 0.11
C2 90 0.528 1.016 8.900 9.418 0.097 0.981 0.185 0.702 8.142 4.596 0.382 1.143 0.306 0.120 2.328 0.634 0.374 0.811 3.685 13.750 0.143 6.695 4.053 3.943 0.688 2.749 0.877 0.787 0.391 0.137 0.073 0.046 0.043 1.114 0.524 0.112 0.035 0.032 0.193 0.046 0.286 0.738 1.532 2.810 1.012 0.293 0.144 0.135 2.394 2.940 0.615 0.029 0.050 2.000 1.243 0.020 0.011 0.000 0.277 0.046 0.202 0.506 0.23 0.46 0.27 0.47 0.41 0.10
C2 91 0.479 0.538 7.534 7.409 0.203 1.667 0.371 1.089 3.503 4.269 1.144 1.524 1.389 0.624 3.953 5.451 0.551 1.771 3.012 11.690 0.390 4.434 2.225 3.466 0.677 1.576 0.605 1.126 0.437 0.090 0.148 0.046 0.026 1.865 0.908 0.417 0.129 0.111 0.902 0.188 0.915 2.235 2.487 3.418 1.292 0.437 0.176 0.106 1.387 3.855 0.652 0.038 0.127 0.507 2.057 0.033 0.017 0.002 0.251 0.182 0.299 0.136 0.42 0.36 0.23 0.19 0.20 0.05

11
Table S7.3: Overview of relative cells abundance per subcommunity (%) in samples taken from reactor D1 (G1 to G68). Time: time points of sampling (day).
Reacto r Time G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 G13 G14 G15 G16 G17 G18 G19 G20 G21 G22 G23 G24 G25 G26 G27 G28 G29 G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G40 G41 G42 G43 G44 G45 G46 G47 G48 G49 G50 G51 G52 G53 G54 G55 G56 G57 G58 G59 G60 G61 G62 G63 G64 G65 G66 G67 G68
D1 0.25 0.009 0.007 0.073 0.030 0.570 0.013 0.110 0.124 0.014 0.003 0.001 0.075 1.540 1.929 0.046 0.060 0.141 0.405 0.014 0.014 0.348 0.012 0.002 0.022 0.024 0.001 0.002 0.051 0.089 0.137 0.074 0.027 0.004 0.245 0.209 46.307 17.886 11.051 4.637 7.923 1.928 0.022 0.016 0.654 0.115 0.043 0.001 0.065 0.001 0.094 0.024 0.029 0.441 0.002 0.005 0.820 0.097 0.151 0.007 0.680 0.010 0.001 0.05 0.00 0.01 0.35 0.15 0.00
D1 1 0.309 0.015 0.136 0.332 0.419 3.741 0.671 2.822 0.480 0.013 0.748 1.200 3.390 5.893 3.367 2.625 0.556 1.448 0.212 0.023 1.453 0.947 0.102 1.053 0.758 0.152 0.058 2.455 0.494 0.058 0.411 0.026 0.015 4.912 1.188 19.577 4.676 2.071 6.488 8.304 2.722 2.519 1.747 0.718 0.410 0.775 0.399 0.069 0.002 1.575 0.147 0.115 0.956 0.007 0.476 0.301 0.437 0.144 0.257 0.630 0.817 0.005 0.28 0.01 0.26 0.26 0.33 0.04
D1 2 0.304 0.015 1.240 3.509 0.851 3.972 0.846 3.730 1.818 0.075 1.871 2.345 3.096 3.638 21.194 4.942 0.335 0.954 1.759 0.206 0.456 3.349 0.047 1.564 0.519 0.661 0.315 1.034 0.394 0.227 0.331 0.017 0.002 1.829 0.548 6.269 1.971 0.966 2.538 2.071 1.280 3.244 4.339 0.298 0.289 0.642 0.637 0.737 0.006 1.482 0.728 0.125 0.314 0.019 1.368 0.085 0.285 0.068 0.177 0.336 0.390 0.039 0.34 0.07 0.40 0.18 0.25 0.06
D1 3 0.143 0.028 4.593 2.456 1.993 4.351 1.521 4.329 0.821 0.219 0.708 1.180 3.544 3.637 17.939 7.254 0.422 1.590 3.561 1.250 0.211 5.763 0.089 1.372 0.203 0.997 0.348 0.884 0.271 0.675 0.381 0.043 0.054 1.232 0.666 1.813 0.670 0.335 2.321 0.605 1.264 5.574 3.708 0.611 0.335 0.576 0.643 1.348 0.015 1.323 1.419 0.159 0.137 0.050 0.703 0.100 0.081 0.020 0.193 0.153 0.484 0.012 0.11 0.03 0.15 0.10 0.19 0.04
D1 5 0.852 0.184 1.791 4.219 5.239 0.338 1.630 7.920 4.219 0.900 0.129 1.438 2.852 0.597 18.152 3.556 0.312 1.258 2.285 1.726 0.290 1.068 0.337 13.224 0.587 0.331 0.144 0.367 0.212 0.231 0.131 0.023 0.426 2.922 0.457 1.079 0.257 0.165 0.992 0.369 1.111 1.532 3.677 0.651 0.465 0.461 0.059 0.580 0.193 4.281 0.621 0.076 0.066 0.264 0.606 0.589 0.027 0.009 0.116 0.073 0.134 0.145 0.11 0.36 0.16 0.18 0.22 0.03
D1 7 2.631 1.332 16.412 8.454 0.482 0.731 0.255 2.157 5.099 2.439 0.497 1.909 1.159 0.383 6.543 1.849 0.197 0.845 2.019 5.635 0.368 0.720 0.265 10.117 2.104 0.193 0.078 0.611 1.601 0.448 0.921 0.219 0.087 2.969 0.401 0.234 0.051 0.036 0.415 0.100 0.381 0.877 2.486 0.148 0.124 0.340 0.068 0.391 2.835 3.512 0.456 0.052 0.032 1.491 0.509 0.187 0.015 0.005 0.268 0.046 0.294 0.566 0.10 1.15 0.10 0.13 0.32 0.15
D1 8 1.148 0.493 31.408 6.627 0.203 0.384 0.287 3.149 6.702 1.126 0.317 1.135 2.658 1.020 1.140 1.046 0.262 1.181 0.943 4.259 0.661 0.894 0.121 5.796 1.500 0.069 0.037 0.503 0.460 0.715 0.457 0.250 0.111 2.515 0.530 0.379 0.069 0.052 0.746 0.160 0.737 1.928 3.605 0.343 0.314 0.171 0.047 0.701 0.926 4.130 0.376 0.024 0.057 1.021 1.029 0.187 0.012 0.005 0.493 0.044 0.202 0.192 0.46 0.33 0.10 0.13 0.53 0.39
D1 9 0.727 0.384 24.529 7.619 0.206 0.151 0.203 2.849 7.918 4.919 0.109 0.354 2.297 0.753 1.694 0.899 0.240 0.617 2.447 6.799 0.732 0.790 0.028 5.716 0.731 0.023 0.019 0.293 0.105 0.120 0.038 0.050 0.060 1.399 0.320 0.258 0.078 0.060 0.538 0.154 0.383 1.173 3.380 1.077 0.988 0.195 0.028 0.195 3.499 3.433 0.286 0.025 0.186 2.383 0.767 0.087 0.037 0.003 0.574 0.082 0.066 0.242 0.30 0.83 0.06 0.34 1.48 0.68
D1 10 0.922 0.525 17.941 7.429 0.212 0.364 1.235 2.211 5.230 6.314 0.140 0.326 2.333 1.009 2.791 1.395 0.738 0.615 4.611 9.873 0.872 0.806 0.019 3.863 0.892 0.052 0.020 0.627 0.112 0.125 0.052 0.029 0.021 1.066 0.520 0.333 0.122 0.080 0.596 0.174 0.432 1.162 2.599 1.630 1.732 0.526 0.042 0.918 4.474 2.659 1.107 0.028 0.271 1.847 0.567 0.124 0.039 0.008 0.343 0.322 0.186 0.188 0.20 0.94 0.11 0.30 0.60 0.05
D1 11 0.273 3.544 6.175 6.553 0.996 0.161 7.945 8.408 0.984 0.812 0.249 3.321 1.940 1.314 2.774 1.771 0.060 0.431 1.626 3.560 2.529 0.519 0.031 0.521 0.372 0.005 0.001 0.426 1.614 0.069 0.893 0.030 0.004 0.588 0.292 1.174 0.325 0.117 0.982 0.786 0.448 0.526 0.512 8.805 13.743 2.127 0.009 1.043 0.852 0.585 1.316 0.545 0.948 0.286 0.162 0.093 0.132 0.014 0.016 1.642 0.183 0.273 0.93 0.26 0.34 0.01 0.02 0.00
D1 12 0.386 1.765 8.443 4.123 0.777 0.654 3.237 1.635 0.255 0.404 0.748 1.134 0.595 0.561 0.736 0.539 0.014 0.085 0.597 1.142 1.101 0.230 0.133 0.148 0.233 0.011 0.008 0.073 0.522 0.092 0.436 0.023 0.007 0.153 0.031 0.345 0.146 0.051 0.188 0.234 0.090 0.179 0.155 37.068 21.683 0.784 0.043 0.884 0.138 0.174 1.429 0.261 1.096 0.142 0.069 0.084 0.960 0.039 0.006 1.723 0.254 0.073 0.46 0.09 0.11 0.00 0.02 0.00
D1 14 2.102 0.070 1.960 1.529 1.209 1.488 5.528 1.715 0.316 0.069 1.326 1.200 2.352 1.547 2.117 1.854 0.087 0.356 0.403 0.211 1.175 0.836 0.204 0.564 1.088 0.032 0.086 0.230 0.780 0.319 0.655 0.098 0.032 0.584 0.167 2.382 1.020 0.470 1.184 0.906 0.737 1.448 0.713 20.178 5.184 1.967 0.058 1.014 0.025 0.838 0.595 0.304 2.076 0.026 0.353 0.146 5.477 2.499 0.039 13.537 0.820 0.009 1.24 0.01 0.40 0.01 0.04 0.01
D1 15 2.074 0.029 2.958 1.902 1.272 1.454 5.314 2.706 0.385 0.138 0.887 1.573 2.799 0.944 1.946 1.834 0.025 0.218 0.586 0.398 1.900 0.424 0.179 0.298 0.672 0.014 0.010 0.126 0.785 0.650 0.680 0.038 0.010 0.479 0.079 1.082 0.477 0.235 0.641 0.543 0.279 0.668 0.479 26.818 12.402 2.309 0.019 1.357 0.052 0.483 0.856 0.247 1.424 0.025 0.183 0.103 2.636 2.086 0.014 6.424 0.735 0.006 1.19 0.01 0.39 0.00 0.03 0.00
D1 17 1.139 0.048 3.625 2.114 3.267 0.536 4.534 3.184 2.815 2.276 0.427 2.003 4.055 0.827 2.212 2.006 0.019 0.244 6.235 0.933 1.723 0.755 0.157 1.908 0.804 0.013 0.003 0.107 0.944 2.101 0.727 0.122 0.032 0.613 0.058 0.598 0.170 0.073 0.569 0.357 0.290 0.536 0.774 20.042 5.800 2.190 0.010 3.544 0.043 1.001 1.170 0.239 1.301 0.264 0.380 3.074 0.209 0.156 0.006 3.004 0.397 0.021 0.79 0.05 0.36 0.00 0.01 0.00
D1 18 0.775 0.015 0.932 1.145 3.189 0.583 3.691 2.470 2.458 2.564 0.491 1.205 2.633 0.761 2.010 1.539 0.006 0.147 7.405 0.186 1.340 0.605 0.158 2.221 0.780 0.005 0.003 0.047 0.664 2.981 0.422 0.107 0.019 0.367 0.035 0.275 0.071 0.030 0.382 0.201 0.124 0.756 0.802 20.027 10.364 1.727 0.003 1.731 0.009 0.798 0.861 0.110 1.104 0.191 0.262 13.104 0.123 0.099 0.003 1.539 0.376 0.023 0.57 0.06 0.31 0.00 0.01 0.00
D1 19 0.627 0.092 4.529 1.592 5.445 0.359 3.607 1.569 1.874 2.788 0.241 0.908 1.401 0.617 1.470 0.921 0.014 0.147 6.667 1.414 1.087 0.416 0.172 1.696 0.501 0.021 0.004 0.063 0.545 8.157 0.244 0.099 0.025 0.319 0.044 0.336 0.095 0.037 0.285 0.249 0.112 0.441 0.489 10.218 7.222 1.445 0.012 7.465 0.013 0.558 1.141 0.092 0.778 0.303 0.164 16.726 0.054 0.032 0.005 0.935 0.095 0.053 0.42 0.13 0.41 0.00 0.01 0.00
D1 21 0.697 0.092 0.606 1.029 11.617 0.535 4.655 1.750 1.611 2.517 0.337 1.138 1.110 0.480 1.586 0.876 0.046 0.214 7.183 0.104 0.751 0.451 0.196 1.818 0.823 0.019 0.013 0.155 0.734 6.117 0.318 0.055 0.220 0.404 0.107 0.519 0.168 0.074 0.232 0.272 0.140 0.354 0.283 9.710 5.739 1.965 0.029 3.930 0.008 0.359 0.893 0.255 0.967 0.260 0.101 20.330 0.027 0.010 0.018 1.536 0.183 0.051 0.43 0.16 0.62 0.00 0.01 0.00
D1 22 0.357 0.090 1.617 1.109 13.873 0.204 3.283 1.576 1.496 3.186 0.161 1.076 1.207 0.527 1.445 0.922 0.008 0.064 9.226 0.377 0.706 0.424 0.106 1.848 0.412 0.017 0.008 0.050 0.718 7.111 0.240 0.042 0.022 0.171 0.022 0.294 0.100 0.040 0.231 0.187 0.100 0.298 0.261 6.815 3.049 1.707 0.006 4.638 0.006 0.228 0.605 0.218 0.563 0.290 0.120 24.376 0.015 0.003 0.004 1.013 0.069 0.036 0.53 0.11 0.38 0.00 0.00 0.00
D1 23 0.336 0.060 3.416 1.430 13.259 0.153 4.339 1.174 1.778 2.394 0.117 1.031 1.012 0.453 1.241 0.756 0.015 0.062 9.122 1.045 0.619 0.481 0.045 1.621 0.466 0.017 0.003 0.037 0.609 9.907 0.298 0.068 0.013 0.156 0.022 0.259 0.080 0.046 0.207 0.160 0.082 0.230 0.220 6.488 2.673 1.586 0.003 7.881 0.009 0.205 1.423 0.199 0.686 0.196 0.104 16.964 0.019 0.008 0.004 1.273 0.043 0.042 0.47 0.13 0.75 0.00 0.00 0.00
D1 24 1.634 0.148 1.586 1.792 13.108 0.375 3.742 0.815 2.137 3.040 0.272 1.164 0.876 0.390 1.075 0.631 0.025 0.130 10.729 0.424 0.542 0.559 0.174 2.373 0.660 0.178 0.016 0.110 0.551 7.249 0.263 0.125 0.247 0.346 0.045 0.452 0.157 0.074 0.266 0.240 0.148 0.207 0.160 6.070 2.162 1.654 0.019 5.938 0.009 0.317 1.479 0.137 0.630 0.305 0.099 18.676 0.020 0.006 0.011 1.187 0.107 0.153 0.44 0.24 1.09 0.00 0.01 0.00
D1 25 0.579 0.062 0.690 1.844 11.096 0.220 3.073 0.964 2.516 3.348 0.122 2.948 0.851 0.469 1.712 0.730 0.025 0.167 11.715 0.098 0.499 0.779 0.044 2.535 0.535 0.019 0.003 0.099 0.804 6.100 0.252 0.177 0.134 0.409 0.057 0.378 0.135 0.062 0.337 0.190 0.202 0.245 0.273 5.389 1.767 3.060 0.017 2.962 0.003 0.337 0.715 0.143 0.805 0.278 0.141 22.424 0.018 0.003 0.010 1.040 0.124 0.039 0.47 0.18 2.57 0.00 0.00 0.00
D1 28 0.237 0.103 1.978 1.372 10.355 0.065 4.144 1.211 2.024 1.871 0.034 4.861 1.637 0.761 2.623 1.748 0.001 0.039 9.344 0.241 0.818 1.580 0.004 1.932 0.201 0.002 0.000 0.015 0.941 7.754 0.197 0.125 0.001 0.130 0.007 0.225 0.050 0.017 0.562 0.222 0.109 0.314 0.457 1.932 0.527 4.296 0.001 5.259 0.010 0.185 1.631 0.155 0.317 0.108 0.224 21.918 0.012 0.004 0.001 0.473 0.064 0.029 0.24 0.07 2.22 0.00 0.00 0.00
D1 29 2.261 0.111 2.295 1.756 5.778 0.274 2.139 1.229 2.158 1.775 0.242 2.527 1.341 0.636 3.164 1.345 0.018 0.199 8.633 0.339 0.617 1.703 0.549 2.650 0.478 0.463 0.009 0.061 0.596 7.000 0.147 0.243 0.287 0.390 0.053 0.642 0.193 0.089 0.639 0.350 0.375 0.296 0.468 1.538 0.840 7.119 0.016 2.805 0.010 0.619 1.301 0.064 0.236 0.099 0.212 24.285 0.008 0.008 0.002 0.365 0.065 0.161 0.14 0.19 3.39 0.00 0.01 0.00
D1 30 0.683 0.083 4.523 2.360 4.960 0.498 1.877 0.652 1.542 1.186 0.242 1.916 1.059 0.649 2.240 0.503 0.031 0.191 7.098 0.395 0.275 1.052 0.313 1.876 0.304 0.040 0.010 0.067 0.472 4.999 0.155 0.221 0.524 0.227 0.068 0.525 0.142 0.067 0.757 0.281 0.441 0.280 0.297 0.298 0.148 16.697 0.013 2.383 0.013 0.393 2.912 0.068 0.068 0.063 0.102 25.703 0.006 0.005 0.006 0.078 0.067 0.063 0.04 0.07 4.71 0.00 0.01 0.00
D1 31 0.414 0.098 9.285 4.066 3.924 0.433 3.215 0.331 1.869 0.937 0.253 2.375 0.871 0.773 1.858 0.513 0.013 0.155 5.378 0.351 0.161 1.345 0.468 1.603 0.385 0.061 0.014 0.064 0.224 7.815 0.077 0.577 0.366 0.264 0.059 0.791 0.280 0.158 0.896 0.391 0.415 0.389 0.333 0.254 0.099 6.588 0.011 3.381 0.009 0.427 2.506 0.022 0.080 0.058 0.145 24.003 0.004 0.006 0.010 0.161 0.072 0.028 0.03 0.08 7.76 0.00 0.01 0.01
D1 35 0.174 0.666 13.717 4.580 2.198 0.061 1.591 0.715 0.689 0.422 0.052 2.160 1.589 1.237 3.126 0.949 0.005 0.073 1.747 1.532 0.295 0.905 0.023 0.552 0.105 0.008 0.000 0.021 0.293 5.179 0.042 1.173 0.037 0.120 0.072 0.830 0.174 0.066 1.473 0.548 0.484 0.551 0.529 0.204 0.047 15.062 0.001 4.587 0.094 0.661 3.655 0.008 0.092 0.231 0.179 15.997 0.003 0.002 0.011 0.095 0.012 0.152 0.02 0.06 8.05 0.00 0.00 0.01
D1 36 0.184 0.718 12.332 3.846 1.538 0.147 1.334 0.931 0.439 0.244 0.094 6.450 2.255 1.362 4.688 1.492 0.008 0.065 1.044 1.006 0.418 1.378 0.121 0.438 0.062 0.025 0.004 0.010 0.658 4.244 0.081 1.114 0.018 0.051 0.050 0.248 0.026 0.006 1.183 0.291 0.233 0.545 0.546 0.233 0.045 18.669 0.002 4.250 0.022 0.441 2.299 0.012 0.108 0.239 0.211 11.214 0.004 0.001 0.006 0.044 0.024 0.197 0.03 0.05 9.96 0.00 0.01 0.01
D1 37 0.209 0.667 14.899 3.960 1.288 0.200 1.497 1.040 0.612 0.200 0.126 7.933 2.581 1.531 4.885 1.885 0.009 0.087 1.006 0.955 0.486 1.650 0.248 0.502 0.090 0.046 0.011 0.026 0.662 3.936 0.073 1.099 0.010 0.086 0.061 0.296 0.040 0.011 1.317 0.349 0.278 0.670 0.655 0.200 0.037 15.479 0.004 3.923 0.011 0.435 2.287 0.011 0.100 0.228 0.279 8.849 0.003 0.001 0.008 0.056 0.028 0.166 0.03 0.05 9.62 0.00 0.01 0.02
D1 38 0.248 0.571 13.003 4.023 1.243 0.248 2.155 0.788 0.616 0.154 0.146 10.852 2.452 1.617 5.573 1.748 0.013 0.115 1.080 0.720 0.423 1.978 0.313 0.523 0.085 0.057 0.006 0.027 0.732 3.687 0.093 0.780 0.003 0.107 0.058 0.288 0.039 0.019 1.383 0.344 0.272 0.689 0.638 0.185 0.022 14.880 0.006 3.878 0.008 0.429 2.482 0.015 0.104 0.232 0.280 6.734 0.002 0.003 0.011 0.044 0.033 0.151 0.03 0.04 10.50 0.00 0.00 0.01
D1 39 0.291 0.469 13.891 4.427 1.131 0.454 1.580 1.263 0.873 0.206 0.235 7.164 2.923 1.664 5.414 1.965 0.007 0.104 1.750 1.084 0.418 2.112 0.323 0.762 0.095 0.064 0.014 0.048 0.481 2.477 0.094 1.057 0.002 0.118 0.078 0.422 0.046 0.028 1.593 0.492 0.360 0.746 0.715 0.147 0.029 16.361 0.007 4.334 0.009 0.502 2.724 0.016 0.099 0.223 0.353 2.406 0.004 0.004 0.012 0.070 0.041 0.137 0.03 0.06 12.96 0.00 0.01 0.02
D1 43 0.082 0.580 15.480 5.316 1.316 0.018 1.573 1.521 1.103 0.198 0.020 4.295 3.422 2.047 3.944 2.826 0.005 0.045 2.669 1.531 0.585 2.130 0.006 1.159 0.071 0.001 0.000 0.021 0.507 1.541 0.113 0.028 0.000 0.044 0.044 0.490 0.047 0.016 2.118 0.521 0.520 1.207 1.207 0.158 0.019 16.987 0.000 4.784 0.045 0.682 4.563 0.013 0.083 0.182 0.488 0.036 0.003 0.006 0.023 0.033 0.017 0.086 0.03 0.03 11.30 0.00 0.01 0.05
D1 44 0.099 0.382 16.558 6.421 1.201 0.079 1.524 1.340 1.282 0.220 0.067 3.022 3.127 1.886 3.621 2.455 0.008 0.070 3.386 1.489 0.516 2.092 0.010 1.516 0.105 0.002 0.000 0.022 0.519 1.138 0.137 0.027 0.002 0.077 0.040 0.564 0.072 0.022 2.081 0.543 0.622 1.177 1.120 0.142 0.019 15.480 0.003 4.455 0.047 0.804 6.100 0.011 0.072 0.140 0.417 0.026 0.003 0.008 0.017 0.039 0.038 0.083 0.03 0.04 11.31 0.00 0.02 0.06
D1 45 0.160 0.227 21.598 7.016 1.390 0.110 8.319 1.358 0.527 0.285 0.070 8.853 3.174 2.282 3.473 2.578 0.010 0.102 0.636 0.810 0.764 1.578 0.018 0.516 0.085 0.006 0.000 0.019 2.093 0.974 0.432 0.018 0.006 0.088 0.074 1.183 0.230 0.092 2.210 0.981 0.505 0.838 0.879 0.307 0.035 7.038 0.000 7.391 0.073 0.480 3.817 0.103 0.250 0.055 0.474 0.065 0.004 0.014 0.022 0.315 0.033 0.023 0.07 0.10 2.67 0.01 0.02 0.07
D1 46 0.359 0.058 14.201 8.118 0.727 0.433 9.419 3.214 0.972 0.254 0.281 8.334 4.172 1.983 4.161 2.957 0.017 0.178 1.225 1.038 0.897 2.046 0.058 1.169 0.180 0.008 0.002 0.069 3.550 1.713 0.797 0.026 0.007 0.206 0.087 1.113 0.267 0.112 1.845 0.822 0.506 0.872 1.111 0.344 0.083 5.986 0.005 5.827 0.041 0.657 3.896 0.244 0.252 0.063 0.612 0.067 0.009 0.012 0.017 0.373 0.131 0.005 0.12 0.07 1.59 0.00 0.01 0.02
D1 49 0.487 1.174 14.251 4.540 0.142 0.378 3.053 2.765 2.787 0.381 0.296 3.038 3.744 1.439 8.180 2.050 0.035 0.446 2.416 1.211 0.746 2.533 0.180 2.459 0.149 0.027 0.008 0.136 2.485 7.966 0.632 4.507 0.007 0.427 0.156 1.528 0.465 0.265 1.829 0.731 1.161 1.004 1.641 0.355 0.102 4.453 0.009 3.032 0.578 1.221 2.417 0.025 0.183 0.343 0.776 0.351 0.006 0.011 0.010 0.552 0.061 0.230 0.12 0.11 1.18 0.00 0.01 0.02
D1 50 0.622 1.351 10.497 4.301 0.090 0.456 2.623 3.078 2.162 0.408 0.422 4.231 2.739 1.896 9.789 2.003 0.018 0.293 2.331 1.362 1.225 2.430 0.270 2.147 0.162 0.012 0.006 0.081 4.705 11.847 1.075 2.746 0.004 0.335 0.084 1.279 0.264 0.111 1.955 0.791 0.865 1.004 1.670 0.677 0.291 3.890 0.008 1.556 0.209 0.966 1.976 0.034 0.361 0.353 0.876 0.239 0.005 0.015 0.003 0.640 0.075 0.230 0.69 0.12 1.03 0.00 0.01 0.01
D1 51 0.742 0.683 8.330 4.276 0.165 0.540 2.732 4.054 2.321 0.501 0.515 4.944 3.458 1.851 15.023 2.477 0.017 0.309 1.879 1.109 1.503 2.589 0.107 2.325 0.242 0.006 0.001 0.049 4.606 7.191 2.286 0.259 0.003 0.316 0.063 0.897 0.198 0.076 1.482 0.630 0.587 0.773 2.722 1.250 0.824 3.410 0.004 2.159 0.018 0.659 1.748 0.090 0.513 0.148 1.214 0.094 0.006 0.008 0.002 0.748 0.125 0.073 1.27 0.20 0.60 0.00 0.00 0.00
D1 52 0.735 0.557 6.912 6.337 0.109 0.549 4.027 2.859 3.511 0.236 0.438 3.477 2.263 1.333 22.362 1.804 0.018 0.249 3.632 0.585 1.177 3.590 0.100 2.543 0.316 0.014 0.000 0.056 4.408 2.380 2.719 0.097 0.035 0.316 0.055 0.968 0.261 0.139 1.024 0.572 0.418 0.534 1.779 0.879 0.563 4.656 0.006 1.620 0.021 0.428 1.921 0.112 0.397 0.177 1.127 0.051 0.017 0.011 0.005 0.568 0.132 0.109 0.84 0.05 0.81 0.00 0.00 0.00
D1 53 0.445 0.312 6.987 3.438 0.418 0.657 4.045 7.468 0.401 0.407 0.250 4.115 2.274 1.145 22.033 3.162 0.004 0.181 0.945 1.185 1.557 0.945 0.015 0.695 0.543 0.003 0.001 0.087 5.679 3.131 5.322 0.025 0.010 0.360 0.037 0.578 0.152 0.058 0.693 0.391 0.202 0.760 2.533 1.045 1.040 3.628 0.008 2.498 0.007 0.274 1.488 0.137 0.481 0.156 0.478 0.055 0.011 0.011 0.002 0.765 0.820 0.037 2.56 0.17 0.67 0.00 0.01 0.00
D1 56 0.397 0.579 5.495 4.952 1.457 0.115 1.995 6.028 0.828 1.176 0.050 3.552 1.174 0.344 25.410 2.650 0.001 0.044 1.457 1.829 0.853 1.518 0.003 1.568 0.215 0.001 0.000 0.033 3.093 7.590 2.980 0.120 0.009 0.122 0.008 0.059 0.008 0.004 0.186 0.063 0.056 0.736 3.555 0.336 0.400 3.459 0.002 4.915 0.017 0.178 0.767 0.072 0.054 0.387 0.762 0.420 0.011 0.025 0.000 0.044 0.152 0.084 0.91 0.69 4.00 0.00 0.00 0.00
D1 57 0.458 0.528 4.681 3.858 2.018 0.134 1.740 6.416 1.054 0.932 0.052 4.143 1.072 0.320 25.531 2.312 0.002 0.046 1.370 1.702 0.492 1.198 0.006 1.352 0.155 0.000 0.000 0.028 1.517 7.712 1.588 0.228 0.012 0.085 0.015 0.098 0.024 0.008 0.215 0.061 0.078 0.638 3.313 0.228 0.284 8.489 0.004 4.566 0.025 0.244 0.892 0.064 0.048 0.361 0.637 0.524 0.007 0.007 0.000 0.048 0.058 0.101 0.62 0.62 4.97 0.00 0.00 0.00
D1 58 0.595 1.051 4.852 5.034 1.859 0.132 1.837 3.033 1.138 1.265 0.096 9.847 0.778 0.225 20.365 2.151 0.002 0.047 1.624 1.462 0.322 2.235 0.010 1.644 0.208 0.000 0.000 0.027 1.355 5.943 0.690 0.163 0.006 0.112 0.012 0.043 0.009 0.003 0.162 0.034 0.075 1.054 3.298 0.182 0.281 7.208 0.005 5.706 0.018 0.451 1.088 0.047 0.024 0.717 0.859 0.469 0.008 0.032 0.001 0.021 0.053 0.186 0.45 0.77 6.62 0.00 0.00 0.00
D1 59 0.421 0.634 4.130 5.660 3.136 0.116 2.697 1.807 1.515 1.176 0.060 13.442 0.561 0.205 16.191 1.549 0.002 0.051 1.851 1.252 0.196 2.453 0.012 1.539 0.126 0.000 0.002 0.030 1.929 5.176 0.316 0.130 0.009 0.105 0.009 0.047 0.009 0.002 0.201 0.036 0.076 0.994 2.980 0.145 0.198 9.526 0.003 6.187 0.009 0.514 1.550 0.056 0.018 0.373 0.923 0.475 0.011 0.016 0.001 0.017 0.023 0.083 0.33 0.78 5.93 0.00 0.00 0.00
D1 60 0.536 0.455 3.433 5.070 3.298 0.203 5.085 2.224 1.315 0.957 0.161 23.110 0.666 0.237 12.696 1.786 0.002 0.038 1.744 0.777 0.184 1.928 0.017 1.310 0.196 0.001 0.001 0.013 3.723 3.191 0.176 0.197 0.007 0.110 0.007 0.041 0.004 0.003 0.228 0.033 0.099 0.953 2.495 0.128 0.167 6.720 0.002 6.290 0.006 0.514 2.012 0.061 0.022 0.331 0.713 1.315 0.017 0.029 0.001 0.018 0.057 0.048 0.23 0.56 2.06 0.00 0.00 0.00
D1 65 0.622 0.188 3.004 3.299 2.880 0.325 3.910 1.739 0.965 0.564 0.251 21.199 0.780 0.320 7.399 1.422 0.003 0.066 1.289 0.386 0.269 1.563 0.088 0.851 0.188 0.003 0.002 0.033 3.365 5.411 0.221 0.161 0.003 0.151 0.016 0.087 0.007 0.003 0.333 0.081 0.158 0.506 0.952 0.087 0.080 7.142 0.001 3.285 0.005 0.379 1.181 0.120 0.032 0.199 0.350 18.620 0.021 0.023 0.004 0.017 0.059 0.021 0.12 0.35 2.84 0.00 0.01 0.00
D1 66 0.676 0.177 3.121 4.362 2.696 0.328 4.244 1.657 0.997 0.757 0.271 23.617 0.896 0.381 6.983 1.354 0.005 0.047 1.377 0.434 0.241 1.616 0.059 0.845 0.166 0.001 0.001 0.024 2.384 5.521 0.240 0.198 0.003 0.127 0.015 0.102 0.010 0.004 0.363 0.107 0.133 0.453 0.821 0.112 0.076 5.288 0.005 3.038 0.006 0.327 1.262 0.100 0.045 0.285 0.351 17.951 0.017 0.029 0.001 0.027 0.108 0.017 0.13 0.38 2.61 0.00 0.01 0.00
D1 67 0.851 0.387 4.123 3.774 2.875 0.358 4.210 1.471 0.918 0.912 0.274 20.841 0.965 0.363 6.524 1.273 0.008 0.073 1.202 0.610 0.238 1.515 0.064 0.782 0.192 0.001 0.001 0.034 2.550 6.062 0.233 0.253 0.005 0.156 0.030 0.095 0.015 0.004 0.365 0.099 0.167 0.424 0.832 0.119 0.070 6.617 0.005 3.386 0.024 0.348 1.260 0.105 0.034 0.527 0.338 17.353 0.016 0.039 0.003 0.018 0.089 0.045 0.13 0.50 2.83 0.00 0.00 0.00
D1 69 0.754 0.213 4.062 4.825 3.729 0.313 4.444 2.239 0.976 0.873 0.233 25.479 1.055 0.410 6.684 1.502 0.006 0.036 1.343 0.734 0.334 1.348 0.023 0.731 0.213 0.000 0.001 0.021 5.100 4.760 0.463 0.232 0.000 0.132 0.011 0.132 0.015 0.009 0.401 0.130 0.126 0.423 0.883 0.189 0.070 5.262 0.003 2.981 0.026 0.267 1.089 0.109 0.055 0.261 0.308 11.786 0.022 0.038 0.003 0.034 0.182 0.032 0.20 0.43 1.24 0.00 0.00 0.00
D1 70 0.645 0.447 5.437 4.764 3.636 0.261 3.688 1.699 1.107 1.131 0.225 25.210 1.043 0.361 7.755 1.565 0.005 0.088 1.804 1.174 0.326 1.763 0.066 1.082 0.194 0.001 0.001 0.033 3.008 3.814 0.280 0.255 0.003 0.143 0.021 0.323 0.068 0.026 0.448 0.196 0.213 0.501 1.038 0.158 0.055 5.349 0.008 3.157 0.105 0.380 1.254 0.088 0.057 0.462 0.440 9.626 0.014 0.008 0.001 0.055 0.061 0.058 0.07 0.44 2.30 0.00 0.00 0.00
D1 71 0.584 1.450 7.688 4.114 3.642 0.140 1.176 1.217 1.474 0.978 0.107 16.881 0.898 0.359 7.852 0.895 0.010 0.128 2.403 1.618 0.227 1.857 0.110 1.475 0.189 0.011 0.005 0.026 3.371 6.751 0.158 0.789 0.012 0.192 0.027 0.422 0.090 0.050 0.601 0.234 0.396 0.371 0.858 0.065 0.026 12.055 0.005 1.832 0.296 0.539 0.983 0.053 0.039 0.632 0.402 6.061 0.013 0.004 0.002 0.032 0.023 0.292 0.05 0.34 4.43 0.00 0.00 0.00
D1 72 2.147 3.833 1.510 4.709 4.233 0.363 1.169 1.637 1.144 1.277 0.326 19.507 1.095 0.767 8.646 1.226 0.007 0.149 1.512 1.068 0.293 1.556 0.470 1.464 0.310 0.331 0.014 0.040 8.290 1.230 0.410 0.071 2.042 0.261 0.040 0.322 0.040 0.022 0.884 0.258 0.462 0.484 0.980 0.108 0.040 11.839 0.008 1.995 0.080 0.607 0.869 0.071 0.074 1.164 0.459 2.131 0.016 0.033 0.002 0.049 0.055 0.983 0.11 0.47 2.23 0.00 0.00 0.00
D1 73 0.969 2.585 1.235 3.080 3.027 0.273 1.109 1.958 1.897 0.986 0.168 17.944 2.122 1.102 10.388 1.764 0.023 0.263 2.144 0.627 0.525 2.298 0.106 2.092 0.298 0.030 0.010 0.089 4.422 1.520 0.214 0.123 0.465 0.330 0.066 0.758 0.138 0.055 1.439 0.443 0.797 0.756 1.296 0.159 0.049 14.102 0.017 2.189 0.034 1.067 0.671 0.072 0.093 0.929 0.589 1.606 0.011 0.034 0.009 0.083 0.064 0.750 0.09 0.28 5.16 0.00 0.01 0.00
D1 74 0.858 2.670 2.414 3.454 2.106 0.247 0.792 1.536 1.768 1.041 0.144 15.971 1.940 1.025 10.114 1.486 0.019 0.272 2.052 0.775 0.357 3.029 0.075 2.264 0.298 0.018 0.009 0.092 1.264 2.032 0.116 0.182 0.333 0.319 0.071 0.747 0.118 0.040 1.499 0.387 0.876 0.891 1.277 0.119 0.037 13.684 0.022 2.738 0.026 1.144 0.866 0.041 0.060 1.128 0.591 1.051 0.008 0.021 0.005 0.050 0.064 0.803 0.07 0.35 10.13 0.00 0.01 0.00
D1 77 0.556 2.465 11.224 4.645 0.661 0.244 0.911 1.539 1.346 0.810 0.162 11.106 2.787 1.581 11.249 1.997 0.020 0.197 1.668 1.194 0.441 2.617 0.234 1.681 0.147 0.028 0.009 0.046 0.550 3.350 0.030 0.225 0.045 0.188 0.063 1.590 0.362 0.145 2.149 0.868 0.911 1.087 1.682 0.151 0.034 8.211 0.007 4.157 0.016 0.883 1.677 0.012 0.130 0.879 0.687 0.453 0.003 0.002 0.006 0.134 0.047 0.543 0.03 0.22 6.88 0.00 0.02 0.01
D1 78 0.429 1.806 11.375 4.815 0.753 0.198 1.346 0.945 1.248 0.713 0.147 10.248 2.137 1.625 12.671 1.627 0.016 0.170 1.500 1.124 0.402 2.527 0.240 1.637 0.122 0.040 0.007 0.043 0.596 3.500 0.030 0.166 0.039 0.137 0.067 1.725 0.424 0.173 2.164 0.915 0.844 0.985 1.787 0.160 0.025 9.439 0.009 5.065 0.016 0.719 1.874 0.011 0.122 0.475 0.753 0.273 0.002 0.004 0.005 0.234 0.035 0.335 0.01 0.23 6.71 0.00 0.01 0.01
D1 79 0.381 1.460 12.791 4.866 0.595 0.172 1.154 1.711 1.279 0.571 0.094 7.396 2.846 1.562 12.849 1.884 0.019 0.154 1.265 1.346 0.652 2.304 0.276 1.512 0.093 0.067 0.014 0.028 0.354 4.538 0.039 0.244 0.086 0.130 0.072 1.660 0.376 0.160 2.017 0.914 0.849 1.023 1.854 0.182 0.035 9.542 0.005 4.800 0.017 0.712 1.683 0.011 0.111 0.475 0.731 0.158 0.001 0.005 0.012 0.175 0.023 0.266 0.03 0.17 7.17 0.00 0.01 0.01
D1 80 0.383 1.695 11.257 4.176 0.469 0.101 0.543 1.727 1.300 0.509 0.054 7.024 3.276 2.032 12.666 2.392 0.011 0.163 1.259 1.468 0.748 2.591 0.159 1.788 0.056 0.056 0.003 0.020 0.283 4.980 0.023 0.255 0.087 0.101 0.058 1.114 0.159 0.044 2.362 0.833 0.908 1.341 2.175 0.209 0.036 10.666 0.001 3.823 0.019 0.885 1.351 0.010 0.126 0.537 0.824 0.095 0.001 0.008 0.008 0.099 0.013 0.352 0.03 0.15 8.09 0.00 0.01 0.01
D1 81 0.569 1.989 13.868 4.831 0.571 0.217 0.660 1.426 1.527 0.509 0.125 4.698 2.520 1.372 13.522 1.827 0.016 0.149 1.682 1.549 0.533 2.577 0.179 2.023 0.084 0.064 0.014 0.032 0.153 5.811 0.015 0.300 0.115 0.123 0.063 1.922 0.573 0.274 1.948 0.897 0.899 1.063 2.098 0.143 0.016 6.924 0.006 3.980 0.024 0.832 1.560 0.010 0.113 0.811 0.898 0.097 0.002 0.004 0.005 0.316 0.019 0.454 0.02 0.15 8.20 0.00 0.02 0.00
D1 84 0.584 1.728 7.248 4.304 2.544 0.201 0.801 0.479 2.100 0.444 0.118 3.579 1.308 1.069 14.437 1.045 0.007 0.111 2.788 1.192 0.255 2.629 0.205 2.129 0.053 0.081 0.007 0.024 0.216 8.740 0.025 0.359 0.169 0.083 0.028 1.838 0.747 0.423 1.425 0.766 0.634 0.653 1.725 0.134 0.029 11.535 0.002 5.862 0.032 0.520 2.052 0.005 0.110 0.684 0.655 0.209 0.001 0.003 0.003 0.541 0.012 0.399 0.01 0.12 7.76 0.00 0.01 0.00
D1 85 0.765 4.773 6.026 3.478 2.707 0.186 0.708 0.986 1.889 0.640 0.098 2.979 1.353 0.809 13.013 1.329 0.010 0.136 1.899 1.432 0.389 2.095 0.270 1.852 0.077 0.117 0.006 0.029 0.259 10.938 0.048 0.415 0.198 0.126 0.044 1.594 0.640 0.337 1.256 0.625 0.631 0.675 1.600 0.101 0.026 11.252 0.004 5.977 0.200 0.656 1.821 0.006 0.070 1.651 0.631 0.381 0.003 0.005 0.001 0.311 0.011 2.037 0.03 0.28 5.09 0.00 0.01 0.00
D1 86 0.581 4.891 4.941 4.753 4.090 0.189 0.875 0.785 2.342 1.961 0.090 3.465 1.140 0.716 14.497 0.942 0.007 0.124 1.587 1.833 0.311 2.012 0.179 2.123 0.074 0.045 0.004 0.018 0.472 9.471 0.079 0.313 0.089 0.102 0.026 1.067 0.387 0.233 0.982 0.460 0.530 0.584 1.762 0.143 0.038 9.451 0.003 5.873 0.248 0.633 1.389 0.007 0.077 1.675 0.672 0.345 0.002 0.006 0.002 0.336 0.014 2.375 0.03 1.06 4.48 0.00 0.01 0.00
D1 87 0.715 8.168 6.310 3.452 2.439 0.152 0.567 0.714 1.675 0.969 0.094 3.013 0.965 0.669 13.113 0.935 0.008 0.134 1.758 1.722 0.267 1.979 0.185 1.918 0.092 0.033 0.003 0.023 0.303 11.640 0.052 0.504 0.041 0.130 0.032 1.417 0.640 0.399 1.016 0.490 0.638 0.617 1.568 0.110 0.020 7.746 0.003 6.101 0.248 0.714 1.277 0.004 0.060 2.544 0.676 0.252 0.003 0.007 0.004 0.323 0.014 3.878 0.02 0.49 3.93 0.00 0.01 0.00
D1 88 0.689 4.846 6.405 4.740 3.820 0.191 1.046 0.498 2.206 0.719 0.117 3.555 1.032 0.838 15.593 0.916 0.011 0.119 1.822 1.440 0.205 2.250 0.154 1.853 0.108 0.022 0.005 0.021 0.361 10.567 0.071 0.392 0.040 0.130 0.041 1.513 0.646 0.380 1.212 0.592 0.539 0.586 1.802 0.137 0.021 7.537 0.002 6.128 0.146 0.566 1.399 0.004 0.102 1.362 0.759 0.374 0.002 0.007 0.002 0.598 0.012 1.640 0.03 0.36 4.70 0.00 0.02 0.00
D1 90 0.641 9.990 6.334 4.050 3.601 0.196 0.544 0.411 2.040 1.130 0.131 2.159 0.608 0.493 13.771 0.557 0.008 0.155 1.330 2.143 0.152 1.860 0.197 1.547 0.103 0.048 0.003 0.030 0.334 11.337 0.078 0.452 0.053 0.140 0.039 0.892 0.382 0.232 0.790 0.358 0.534 0.417 1.530 0.080 0.023 7.841 0.005 4.946 0.831 0.619 1.071 0.003 0.054 3.163 0.638 0.442 0.004 0.006 0.002 0.295 0.019 4.066 0.01 0.39 3.68 0.00 0.01 0.00
D1 91 1.008 7.465 6.958 5.760 1.838 0.358 0.608 0.436 2.389 2.162 0.281 2.240 0.458 0.359 15.110 0.446 0.009 0.124 2.033 2.814 0.122 2.172 0.202 2.117 0.115 0.027 0.005 0.022 0.331 11.901 0.057 0.421 0.044 0.101 0.024 0.791 0.350 0.214 0.602 0.297 0.434 0.372 1.605 0.075 0.017 5.932 0.009 5.453 0.396 0.446 1.267 0.006 0.048 2.122 0.628 0.266 0.002 0.005 0.002 0.243 0.035 3.493 0.03 1.32 3.02 0.00 0.01 0.00

12
Table S7.4: Overview of relative cells abundance per subcommunity (%) in samples taken from reactor D2 (G1 to G68). Time: time points of sampling (day).
Reacto r Time G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 G13 G14 G15 G16 G17 G18 G19 G20 G21 G22 G23 G24 G25 G26 G27 G28 G29 G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G40 G41 G42 G43 G44 G45 G46 G47 G48 G49 G50 G51 G52 G53 G54 G55 G56 G57 G58 G59 G60 G61 G62 G63 G64 G65 G66 G67 G68
D2 0.25 0.005 0.005 0.076 0.029 0.367 0.004 0.080 0.085 0.008 0.002 0.001 0.056 1.312 1.971 0.033 0.051 0.127 0.407 0.008 0.012 0.252 0.007 0.004 0.018 0.020 0.001 0.001 0.036 0.066 0.078 0.031 0.018 0.002 0.178 0.182 47.239 18.153 11.167 4.820 7.739 1.854 0.013 0.010 0.558 0.096 0.051 0.001 0.045 0.001 0.068 0.019 0.020 0.444 0.002 0.007 0.407 0.091 0.147 0.006 0.906 0.005 0.001 0.05 0.00 0.01 0.35 0.18 0.00
D2 1 0.222 0.022 0.056 0.131 0.272 3.023 0.273 2.679 0.312 0.011 0.466 0.876 4.058 5.726 1.470 2.238 0.667 1.497 0.059 0.010 1.397 0.335 0.072 0.882 0.581 0.092 0.035 2.433 0.517 0.059 0.338 0.022 0.007 4.457 1.270 22.780 5.816 2.516 7.152 9.819 2.575 2.337 1.135 0.912 0.431 0.416 0.199 0.028 0.002 1.449 0.039 0.079 1.235 0.004 0.236 0.219 0.552 0.199 0.228 0.913 1.001 0.005 0.25 0.01 0.11 0.37 0.39 0.04
D2 2 0.110 0.009 1.361 1.937 0.554 5.850 0.725 3.059 1.417 0.051 0.856 0.942 2.697 2.788 18.787 6.149 0.321 0.900 3.166 0.241 0.710 5.919 0.027 2.469 0.204 0.772 0.310 0.948 0.576 0.158 0.363 0.015 0.002 1.436 0.525 5.762 2.091 0.951 3.001 1.613 1.419 4.477 4.857 0.258 0.215 0.586 0.966 0.613 0.005 1.946 0.801 0.112 0.264 0.004 1.455 0.053 0.296 0.068 0.194 0.237 0.430 0.004 0.21 0.02 0.22 0.18 0.27 0.07
D2 3 0.081 0.017 3.684 2.385 1.181 6.413 1.314 2.061 0.872 0.164 0.328 0.635 2.789 3.399 12.617 7.277 0.415 1.525 3.442 1.183 0.205 8.312 0.027 1.632 0.115 1.118 0.568 0.953 0.280 0.317 0.222 0.023 0.023 0.913 0.656 2.140 0.835 0.459 3.596 0.623 1.781 7.316 4.902 0.767 0.501 0.554 0.984 1.054 0.017 2.498 1.592 0.107 0.146 0.027 1.173 0.087 0.075 0.022 0.195 0.183 0.575 0.007 0.13 0.02 0.15 0.10 0.19 0.04
D2 5 0.137 0.032 2.058 1.094 1.448 0.257 0.983 8.217 0.628 0.139 0.069 4.252 3.330 2.271 1.959 0.675 0.056 0.150 0.543 0.299 5.534 0.478 0.181 0.485 0.114 0.325 0.094 0.170 7.982 0.199 1.810 0.032 0.103 0.169 0.092 0.773 0.216 0.104 0.850 0.574 0.241 0.313 0.274 22.024 18.078 1.670 0.054 0.361 0.014 0.322 0.415 0.057 2.075 0.033 0.111 0.246 0.167 0.162 0.028 2.026 0.045 0.020 2.06 0.04 0.12 0.05 0.08 0.02
D2 7 0.135 0.061 2.069 0.729 0.175 0.239 0.825 5.179 0.220 0.245 0.111 7.111 0.839 0.447 0.827 0.473 0.069 0.192 0.424 0.389 1.807 0.171 0.496 0.534 0.140 0.283 0.147 0.179 22.573 0.251 19.379 0.053 0.111 0.320 0.087 0.462 0.161 0.084 0.226 0.232 0.198 0.269 0.222 15.319 6.797 2.545 0.055 0.351 0.015 0.514 0.252 0.147 0.812 0.075 0.084 0.030 0.088 0.048 0.060 0.737 0.048 0.017 2.52 0.02 0.10 0.08 0.09 0.05
D2 8 0.549 0.109 1.397 0.779 0.707 0.715 0.395 2.732 0.764 0.243 0.677 0.916 3.300 1.255 0.632 0.739 0.157 0.663 0.471 0.348 6.118 0.287 0.885 2.335 0.749 1.207 0.429 0.586 2.506 0.298 1.018 0.051 0.808 1.463 0.331 1.381 0.342 0.191 1.049 0.874 0.522 1.471 0.464 28.305 15.937 0.453 0.193 0.296 0.063 1.771 0.246 0.156 1.411 0.119 0.091 0.215 0.156 0.090 0.204 1.008 0.241 0.140 5.04 0.21 0.06 0.23 0.33 0.12
D2 9 0.183 0.049 2.999 1.164 1.196 0.524 0.683 2.042 0.282 0.139 0.305 1.240 2.115 1.479 0.722 0.505 0.041 0.168 0.326 0.516 2.941 0.146 0.134 0.805 0.236 0.612 0.208 0.272 1.987 2.093 0.668 0.051 0.104 0.361 0.080 1.083 0.329 0.155 0.494 0.678 0.189 0.251 0.199 36.775 19.291 0.645 0.113 1.691 0.040 0.425 1.023 0.276 2.619 0.046 0.051 0.762 0.129 0.058 0.057 3.233 0.076 0.052 1.45 0.06 0.14 0.11 0.09 0.03
D2 10 0.156 0.057 6.621 1.728 4.485 0.664 2.033 1.077 0.240 0.118 0.370 1.419 0.506 0.478 0.624 0.341 0.030 0.108 0.333 1.058 0.822 0.133 0.100 0.548 0.194 0.280 0.088 0.178 2.346 9.547 0.966 0.564 0.040 0.363 0.051 0.399 0.100 0.052 0.134 0.241 0.076 0.193 0.191 26.898 17.025 0.926 0.102 4.718 0.070 0.292 1.959 0.374 1.426 0.036 0.045 3.341 0.064 0.023 0.024 1.656 0.073 0.040 0.60 0.04 0.15 0.02 0.04 0.01
D2 11 0.177 0.050 8.762 2.779 4.433 0.337 9.130 1.956 0.179 0.081 0.370 2.906 0.712 0.252 1.089 1.250 0.017 0.067 0.315 1.623 0.632 0.176 0.056 0.190 0.318 0.130 0.041 0.154 1.610 12.825 0.620 0.961 0.041 0.427 0.038 0.165 0.040 0.020 0.091 0.085 0.079 0.127 0.192 15.418 4.219 2.647 0.048 9.688 0.029 0.129 2.082 0.436 0.831 0.033 0.106 3.079 0.701 0.177 0.008 0.873 0.134 0.029 1.43 0.02 2.35 0.01 0.02 0.00
D2 12 0.552 0.038 6.607 2.457 6.475 0.435 10.871 3.183 0.270 0.112 0.384 6.098 1.804 0.208 1.746 2.491 0.011 0.100 0.323 1.161 0.656 0.201 0.048 0.346 0.450 0.028 0.007 0.092 1.509 12.146 0.836 0.734 0.041 0.366 0.038 0.104 0.039 0.017 0.155 0.059 0.078 0.189 0.449 11.756 1.415 2.507 0.016 9.762 0.021 0.410 2.124 0.332 0.683 0.027 0.175 2.860 0.161 0.073 0.004 0.769 0.228 0.026 1.34 0.03 1.35 0.00 0.01 0.00
D2 14 5.165 0.054 5.159 2.212 10.369 2.665 8.727 2.058 0.569 0.118 1.722 3.820 1.160 0.454 2.476 1.024 0.021 0.162 0.511 0.992 0.536 0.472 0.465 0.607 0.981 0.013 0.006 0.079 1.461 8.413 1.613 0.325 0.075 0.391 0.057 0.195 0.061 0.021 0.367 0.124 0.168 0.280 0.380 9.038 1.550 4.882 0.014 6.893 0.016 0.388 2.294 0.510 0.815 0.075 0.169 2.991 0.110 0.050 0.006 1.073 0.910 0.212 0.32 0.17 0.98 0.00 0.01 0.00
D2 15 5.349 0.063 6.282 2.623 6.715 3.391 8.030 1.811 0.592 0.139 2.208 3.771 1.342 0.304 3.179 1.201 0.011 0.094 0.838 1.463 0.597 0.759 0.929 0.625 0.675 0.011 0.004 0.059 1.277 9.439 1.275 0.650 0.074 0.298 0.018 0.178 0.064 0.026 0.169 0.109 0.070 0.136 0.342 8.751 1.922 4.112 0.019 8.527 0.019 0.313 2.459 0.431 0.924 0.053 0.179 2.096 0.078 0.020 0.003 0.826 0.914 0.083 0.21 0.08 0.77 0.00 0.01 0.00
D2 17 4.194 0.039 4.409 3.145 4.655 3.293 5.272 1.054 1.806 0.162 2.605 2.092 0.834 0.333 12.268 1.976 0.003 0.089 4.762 1.060 0.276 4.634 0.488 3.063 0.913 0.022 0.004 0.054 0.594 5.450 0.515 0.310 0.086 0.364 0.019 0.119 0.035 0.013 0.271 0.094 0.102 0.516 1.227 8.076 1.281 1.767 0.017 6.578 0.030 0.627 2.461 0.345 0.885 0.044 0.688 1.014 0.035 0.010 0.004 0.684 1.317 0.042 0.15 0.06 0.65 0.00 0.01 0.00
D2 18 5.836 0.036 4.688 3.339 5.042 3.338 3.782 0.833 2.042 0.167 1.615 1.116 1.059 0.497 14.279 1.456 0.003 0.087 4.649 1.363 0.237 4.422 0.200 3.462 1.622 0.029 0.003 0.039 0.408 5.514 0.360 0.215 0.072 0.781 0.014 0.161 0.028 0.017 0.434 0.117 0.174 0.623 1.578 6.600 1.631 1.375 0.009 5.640 0.026 0.888 2.438 0.197 0.624 0.028 0.752 0.869 0.023 0.009 0.001 0.482 2.021 0.050 0.10 0.07 0.42 0.00 0.01 0.00
D2 19 5.155 0.071 5.083 2.529 4.420 4.417 2.673 0.897 2.287 0.262 2.850 2.413 1.598 0.842 12.523 1.313 0.011 0.247 1.115 2.089 0.281 2.092 1.461 2.203 0.552 0.019 0.038 0.080 0.585 7.904 0.257 0.377 0.059 0.444 0.047 0.290 0.049 0.017 0.693 0.227 0.304 0.623 1.665 7.157 2.120 3.089 0.024 7.180 0.038 0.927 1.511 0.129 0.767 0.068 0.614 0.951 0.037 0.005 0.007 0.578 0.500 0.063 0.08 0.08 0.99 0.01 0.01 0.00
D2 21 9.148 0.119 5.170 1.977 5.944 6.041 2.698 1.313 0.849 0.255 3.666 1.932 1.899 1.333 4.125 1.657 0.016 0.426 1.011 1.878 0.398 1.273 1.754 1.656 0.690 0.025 0.021 0.096 0.769 10.395 0.209 0.361 0.074 0.558 0.079 1.581 0.439 0.215 1.543 0.823 0.810 0.798 1.218 3.123 0.529 3.686 0.030 7.080 0.029 1.338 1.606 0.118 0.682 0.066 0.464 1.097 0.017 0.007 0.004 0.930 0.779 0.103 0.08 0.10 0.87 0.00 0.01 0.01
D2 22 4.657 0.138 4.215 2.475 7.678 3.612 3.051 2.170 1.707 0.297 1.818 2.589 2.676 1.565 9.876 2.119 0.016 0.367 1.257 1.357 0.563 1.844 0.938 1.859 0.409 0.006 0.013 0.088 0.864 8.873 0.262 0.287 0.028 0.422 0.061 1.236 0.319 0.141 1.745 0.730 0.785 1.045 1.609 2.461 0.263 5.370 0.021 5.997 0.023 1.259 1.721 0.167 0.427 0.086 0.711 1.259 0.018 0.004 0.002 0.855 0.330 0.037 0.09 0.07 1.04 0.00 0.01 0.00
D2 23 3.560 0.110 3.738 2.149 5.411 3.131 2.695 1.524 2.353 0.149 2.043 2.381 2.820 1.719 15.611 2.326 0.016 0.261 1.217 1.116 0.537 2.848 0.867 2.453 0.424 0.009 0.031 0.083 0.368 8.838 0.141 0.353 0.010 0.310 0.059 1.234 0.300 0.143 1.895 0.799 0.711 1.120 2.000 1.316 0.188 4.981 0.019 5.207 0.030 0.921 1.612 0.098 0.478 0.066 0.866 0.809 0.010 0.004 0.003 0.793 0.394 0.018 0.06 0.03 2.22 0.00 0.01 0.00
D2 24 0.577 0.157 3.603 2.233 3.615 1.044 2.028 1.550 2.372 0.161 0.589 3.194 3.244 1.928 19.762 2.828 0.040 0.560 1.636 1.234 0.595 4.088 0.269 3.193 0.356 0.015 0.060 0.246 0.266 8.149 0.139 0.472 0.005 0.661 0.185 1.092 0.210 0.105 1.949 0.653 0.918 1.469 2.638 0.675 0.106 4.822 0.021 4.713 0.034 1.169 1.425 0.114 0.310 0.087 1.174 0.575 0.013 0.005 0.006 0.546 0.173 0.031 0.05 0.03 3.83 0.00 0.01 0.00
D2 25 1.326 0.163 2.920 3.236 4.107 1.402 2.236 2.388 2.311 0.290 0.897 4.644 4.107 2.458 17.283 3.639 0.037 0.629 1.901 1.158 0.696 3.276 0.263 2.220 0.531 0.014 0.026 0.220 1.125 4.555 0.157 0.300 0.011 0.740 0.156 1.108 0.216 0.083 2.391 0.752 1.085 1.564 2.947 0.712 0.108 4.483 0.031 4.081 0.061 1.409 2.173 0.129 0.286 0.181 1.305 0.547 0.015 0.006 0.005 0.360 0.263 0.047 0.08 0.08 2.06 0.00 0.01 0.00
D2 28 1.980 0.177 3.066 2.189 4.708 1.463 1.979 2.755 1.935 0.225 0.812 10.384 2.746 1.274 14.089 2.953 0.003 0.131 1.189 0.971 0.564 2.684 0.117 1.564 0.527 0.009 0.001 0.037 2.747 6.847 0.239 0.188 0.007 0.296 0.022 0.328 0.062 0.021 1.060 0.313 0.297 1.044 1.860 0.265 0.075 10.094 0.004 4.331 0.012 0.590 1.139 0.103 0.109 0.076 0.763 0.759 0.007 0.001 0.002 0.088 0.791 0.029 0.04 0.09 4.75 0.00 0.01 0.00
D2 29 1.137 0.301 3.928 5.761 3.599 0.692 0.984 0.906 2.999 0.276 0.574 4.014 2.059 1.392 11.981 1.725 0.010 0.234 2.797 0.844 0.266 2.848 0.980 1.754 0.340 0.107 0.028 0.062 0.680 10.337 0.146 1.672 0.009 0.260 0.043 1.009 0.205 0.085 1.759 0.647 0.724 1.082 1.461 0.124 0.024 12.220 0.007 2.305 0.203 0.915 1.930 0.069 0.077 0.115 0.731 1.039 0.006 0.001 0.000 0.118 0.088 0.090 0.02 0.08 7.11 0.00 0.00 0.00
D2 30 1.216 0.275 6.173 4.116 3.388 0.846 1.408 0.663 2.365 0.252 0.584 3.616 1.347 0.859 9.309 1.229 0.014 0.224 2.196 1.504 0.178 2.260 0.782 1.129 0.458 0.062 0.019 0.094 0.529 16.562 0.165 2.232 0.002 0.475 0.050 0.568 0.116 0.058 1.018 0.361 0.429 0.551 0.825 0.070 0.021 14.544 0.014 2.816 0.130 0.622 1.787 0.184 0.055 0.089 0.449 0.703 0.008 0.003 0.003 0.074 0.198 0.076 0.02 0.15 7.48 0.00 0.00 0.00
D2 31 4.276 0.036 4.444 3.169 2.538 1.739 0.771 0.253 0.150 0.090 0.958 3.469 0.378 0.143 4.494 0.500 0.019 0.136 1.494 0.791 0.071 1.098 1.279 0.194 0.833 0.040 0.024 0.200 0.144 9.789 0.055 0.326 0.002 0.448 0.065 0.075 0.031 0.016 0.276 0.025 0.149 0.295 0.437 0.039 0.047 25.075 0.052 2.477 0.010 0.269 3.991 0.047 0.006 0.034 0.098 1.004 0.006 0.003 0.014 0.101 0.579 0.005 0.02 0.08 20.30 0.00 0.01 0.01
D2 35 0.705 0.192 5.067 4.748 0.871 0.646 0.479 0.334 4.513 0.437 0.330 6.582 0.939 0.484 7.423 0.594 0.046 0.620 6.200 1.128 0.114 3.770 0.502 3.924 0.679 0.106 0.034 0.359 0.397 8.044 0.064 0.977 0.003 1.009 0.232 0.126 0.043 0.039 0.596 0.069 0.319 1.223 1.655 0.097 0.034 14.052 0.035 0.951 0.144 1.392 1.179 0.031 0.013 0.090 0.631 0.304 0.019 0.007 0.019 0.020 0.279 0.126 0.02 0.25 13.66 0.01 0.01 0.01
D2 36 0.077 0.015 2.426 3.462 0.699 0.125 1.960 9.312 0.378 0.107 0.090 12.805 2.654 1.854 5.495 4.675 0.053 0.235 1.490 0.700 4.411 1.349 0.041 0.560 0.060 0.002 0.001 0.031 9.063 0.223 0.536 0.030 0.001 0.189 0.186 0.243 0.075 0.038 0.466 0.284 0.091 0.538 1.089 7.673 6.146 7.894 0.006 0.264 0.014 0.427 3.151 0.057 1.009 0.018 0.494 0.068 0.078 0.012 0.048 1.318 0.037 0.003 0.24 0.01 2.70 0.03 0.09 0.09
D2 37 0.270 0.008 3.410 2.178 2.340 0.221 3.410 8.749 0.786 0.063 0.077 7.895 3.168 2.027 5.191 3.161 0.039 0.258 1.363 0.733 5.355 1.084 0.114 0.881 0.375 0.013 0.001 0.104 9.254 1.281 1.099 0.021 0.000 0.467 0.230 0.347 0.058 0.023 0.619 0.348 0.194 0.183 0.688 6.250 5.801 9.321 0.004 2.857 0.004 0.447 2.513 0.029 0.747 0.013 0.455 0.041 0.014 0.006 0.038 0.439 0.124 0.001 0.38 0.01 2.35 0.01 0.04 0.02
D2 38 0.214 0.016 8.861 1.785 1.279 0.117 1.648 5.845 1.214 0.085 0.081 4.420 3.767 2.343 3.284 1.649 0.030 0.206 1.356 1.434 4.184 0.953 0.077 1.200 0.199 0.008 0.003 0.053 6.329 16.841 0.778 1.136 0.001 0.353 0.164 0.576 0.103 0.041 0.805 0.612 0.225 0.223 0.453 7.167 5.100 4.969 0.001 1.012 0.143 0.619 1.480 0.021 1.286 0.017 0.189 0.425 0.012 0.002 0.022 1.009 0.020 0.005 0.52 0.02 0.98 0.00 0.02 0.01
D2 39 0.041 0.002 2.200 1.150 1.162 0.031 4.157 6.153 0.220 0.021 0.028 5.291 1.636 2.672 1.724 1.054 0.010 0.072 0.306 0.163 6.289 0.414 0.010 0.320 0.022 0.001 0.000 0.005 9.318 0.331 0.741 0.022 0.002 0.029 0.010 0.172 0.042 0.018 0.312 0.243 0.054 0.135 0.271 19.709 14.722 5.935 0.001 2.682 0.008 0.133 3.561 0.113 1.812 0.008 0.116 0.046 0.029 0.010 0.006 2.166 0.013 0.002 1.29 0.00 0.77 0.00 0.01 0.00
D2 43 0.153 0.060 10.168 0.987 0.712 0.087 1.015 6.055 0.926 0.102 0.058 6.875 1.843 2.492 2.464 1.541 0.063 0.165 0.651 1.960 3.621 0.444 0.040 1.148 0.130 0.004 0.003 0.020 11.713 14.738 0.823 1.881 0.002 0.102 0.041 0.447 0.070 0.022 1.009 0.732 0.146 0.396 0.515 7.920 4.942 3.733 0.002 0.904 0.351 0.725 1.011 0.010 1.145 0.065 0.140 0.267 0.019 0.008 0.011 1.451 0.010 0.017 0.38 0.05 0.40 0.00 0.01 0.00
D2 44 0.055 0.008 4.030 1.017 0.841 0.022 1.079 8.102 0.674 0.076 0.027 9.509 2.181 2.056 2.638 1.947 0.069 0.125 0.659 0.916 3.275 0.529 0.007 0.653 0.029 0.002 0.000 0.012 18.909 1.278 0.952 0.071 0.002 0.034 0.027 0.328 0.071 0.031 0.869 0.590 0.080 0.302 0.558 11.056 7.830 4.448 0.000 2.869 0.009 0.228 1.382 0.036 2.168 0.015 0.211 0.068 0.022 0.005 0.011 1.948 0.008 0.006 2.48 0.03 0.51 0.00 0.00 0.00
D2 45 0.083 0.010 1.768 1.467 0.664 0.037 2.654 7.333 0.520 0.040 0.030 5.117 1.698 0.923 2.711 1.143 0.161 0.182 0.475 0.109 2.584 0.583 0.005 0.788 0.045 0.000 0.000 0.011 21.638 0.139 2.044 0.007 0.002 0.047 0.038 0.126 0.034 0.018 0.296 0.173 0.051 0.174 0.601 15.583 11.475 5.718 0.001 0.675 0.006 0.131 1.469 0.044 1.932 0.010 0.215 0.046 0.020 0.012 0.013 1.952 0.029 0.011 3.43 0.01 0.62 0.00 0.04 0.01
D2 46 0.088 0.010 2.370 2.487 0.202 0.068 0.836 8.834 1.588 0.085 0.055 7.071 2.753 1.525 5.571 2.676 0.060 0.159 1.473 0.383 2.338 0.977 0.008 1.711 0.063 0.001 0.000 0.030 16.041 0.088 1.436 0.008 0.003 0.107 0.050 0.230 0.048 0.020 0.543 0.392 0.113 0.483 1.992 13.750 6.785 4.620 0.002 0.249 0.016 0.325 1.631 0.052 2.706 0.018 0.405 0.026 0.028 0.010 0.016 2.480 0.040 0.004 1.34 0.01 0.48 0.01 0.02 0.01
D2 49 0.207 0.025 3.437 1.356 0.247 0.138 0.399 8.935 0.829 0.273 0.069 4.708 5.048 2.366 11.113 5.132 0.151 0.168 0.964 0.990 3.673 1.226 0.009 1.322 0.325 0.002 0.001 0.083 4.651 0.771 0.398 0.027 0.004 0.530 0.048 0.282 0.090 0.049 0.297 0.260 0.072 1.020 3.596 13.001 7.709 5.898 0.003 0.589 0.122 0.143 0.929 0.015 1.497 0.073 0.539 0.040 0.023 0.017 0.026 2.374 0.155 0.009 0.50 0.03 0.96 0.02 0.04 0.01
D2 50 0.392 0.029 8.628 1.450 0.224 0.286 0.360 13.148 0.261 0.326 0.237 4.839 6.181 0.807 4.973 3.658 0.062 0.066 0.770 2.322 2.434 0.242 0.065 0.217 0.301 0.007 0.001 0.026 8.645 11.982 0.310 1.315 0.000 0.276 0.013 0.111 0.026 0.015 0.226 0.117 0.092 0.634 2.190 6.451 3.892 3.835 0.005 0.514 0.232 0.103 1.468 0.013 0.917 0.053 0.159 0.169 0.014 0.009 0.005 1.211 0.191 0.006 1.89 0.02 0.55 0.00 0.03 0.00
D2 51 0.026 0.011 0.796 0.646 1.099 0.009 2.508 8.988 0.142 0.029 0.011 5.928 2.372 1.211 2.338 2.221 0.026 0.020 0.280 0.091 4.772 0.195 0.004 0.107 0.031 0.002 0.000 0.005 9.410 0.195 0.435 0.026 0.002 0.041 0.003 0.062 0.008 0.005 0.124 0.096 0.014 0.163 1.027 20.758 14.019 5.568 0.000 1.440 0.006 0.026 1.691 0.092 2.917 0.010 0.079 0.103 0.123 0.029 0.004 2.584 0.010 0.002 4.08 0.00 0.96 0.00 0.01 0.00
D2 52 0.044 0.006 0.388 0.404 0.239 0.012 0.937 5.776 0.102 0.059 0.013 4.568 2.439 5.948 2.642 1.638 0.066 0.026 0.153 0.048 12.036 0.236 0.002 0.127 0.018 0.001 0.001 0.011 4.399 0.109 0.184 0.007 0.000 0.033 0.006 0.193 0.027 0.008 0.276 0.247 0.015 0.139 0.627 23.215 19.705 4.236 0.003 0.397 0.002 0.024 0.686 0.011 1.729 0.019 0.079 0.043 0.034 0.011 0.008 3.078 0.009 0.001 1.67 0.00 0.80 0.00 0.02 0.00
D2 53 0.407 0.058 7.834 2.623 1.898 0.164 0.545 3.188 0.143 0.505 0.162 2.713 0.769 1.900 1.081 0.693 0.030 0.095 0.285 5.280 2.921 0.279 0.034 0.288 0.176 0.023 0.007 0.062 2.875 5.129 0.192 0.036 0.016 0.161 0.032 0.260 0.040 0.012 0.174 0.357 0.070 0.150 0.212 22.078 8.725 0.659 0.015 6.922 0.030 0.123 0.672 0.035 2.390 0.198 0.122 0.197 0.086 0.048 0.021 1.845 0.123 0.045 11.38 0.07 0.31 0.01 0.02 0.00
D2 56 0.664 0.043 10.906 5.262 5.210 0.405 1.783 0.962 1.148 0.736 0.313 1.300 1.396 0.472 1.818 0.793 0.026 0.184 0.964 6.204 0.514 0.644 0.042 0.660 0.617 0.028 0.024 0.168 0.551 10.480 0.108 0.079 0.224 0.418 0.055 0.238 0.068 0.033 0.222 0.141 0.134 0.312 1.085 13.049 5.926 1.476 0.033 11.589 0.018 0.375 1.755 0.029 1.383 0.133 0.313 0.913 0.069 0.053 0.011 1.295 0.326 0.082 0.70 0.20 2.82 0.00 0.01 0.00
D2 57 0.771 0.121 6.315 4.925 4.797 0.249 2.103 0.811 1.315 0.787 0.331 1.724 0.936 0.424 8.705 0.946 0.008 0.120 2.744 4.403 0.364 3.177 0.063 2.045 0.609 0.051 0.009 0.083 0.725 7.213 0.232 0.117 0.103 0.345 0.035 0.265 0.070 0.027 0.188 0.195 0.093 0.242 1.637 9.258 3.901 4.182 0.012 8.450 0.031 0.394 2.948 0.035 0.889 0.147 0.747 0.813 0.035 0.042 0.004 1.192 0.111 0.087 0.74 0.43 5.12 0.00 0.01 0.00
D2 58 0.596 0.186 4.542 6.804 2.995 0.233 1.211 0.478 1.849 0.878 0.204 4.002 0.506 0.208 12.141 0.783 0.014 0.132 4.853 2.640 0.184 4.968 0.049 2.417 0.448 0.036 0.003 0.089 0.355 5.403 0.117 0.097 0.054 0.282 0.037 0.160 0.049 0.025 0.146 0.065 0.096 0.226 1.484 4.246 1.882 6.930 0.009 5.591 0.024 0.361 2.467 0.026 0.436 0.128 0.830 0.575 0.007 0.006 0.006 0.629 0.135 0.086 0.43 0.87 12.27 0.00 0.00 0.00
D2 59 0.457 0.238 3.124 8.680 3.371 0.169 1.749 0.415 2.326 0.817 0.164 8.080 0.371 0.131 12.841 0.844 0.012 0.069 4.507 1.837 0.112 4.261 0.029 2.053 0.278 0.018 0.003 0.047 0.371 3.636 0.096 0.033 0.050 0.181 0.023 0.074 0.033 0.020 0.111 0.035 0.075 0.279 1.390 2.111 0.868 7.954 0.005 5.411 0.027 0.264 2.231 0.027 0.110 0.133 0.673 0.464 0.010 0.005 0.005 0.307 0.089 0.059 0.30 1.07 14.45 0.00 0.00 0.00
D2 60 0.364 0.141 1.575 8.741 3.879 0.137 3.170 0.974 4.026 0.279 0.104 18.825 0.527 0.156 13.342 1.610 0.003 0.064 4.112 0.897 0.150 3.572 0.014 1.574 0.306 0.011 0.001 0.033 1.061 1.525 0.138 0.011 0.036 0.215 0.014 0.084 0.037 0.021 0.136 0.056 0.077 0.505 1.673 1.217 0.443 8.712 0.004 3.482 0.023 0.297 2.001 0.066 0.071 0.090 0.673 0.265 0.012 0.007 0.002 0.200 0.157 0.043 0.28 0.25 7.52 0.00 0.00 0.00
D2 65 0.203 0.325 0.758 14.605 1.751 0.015 1.130 0.607 7.636 0.438 0.026 15.708 0.428 0.199 9.835 0.863 0.004 0.070 4.548 1.545 0.069 2.432 0.005 1.742 0.178 0.004 0.000 0.013 2.584 1.027 0.217 0.038 0.008 0.160 0.010 0.152 0.042 0.023 0.266 0.069 0.198 0.426 1.233 0.093 0.042 16.596 0.001 0.881 0.065 0.449 1.003 0.072 0.019 0.097 0.606 0.315 0.013 0.005 0.000 0.023 0.014 0.034 0.04 0.42 7.62 0.00 0.00 0.00
D2 66 0.198 0.280 0.719 16.370 1.761 0.011 1.119 0.653 8.221 0.415 0.026 16.786 0.431 0.210 9.820 0.973 0.004 0.053 4.506 1.768 0.082 2.359 0.002 1.493 0.133 0.003 0.001 0.011 2.759 1.061 0.222 0.025 0.008 0.143 0.004 0.162 0.041 0.031 0.252 0.075 0.158 0.452 1.233 0.071 0.038 14.534 0.001 0.697 0.073 0.367 1.053 0.072 0.015 0.087 0.567 0.287 0.013 0.003 0.000 0.027 0.017 0.041 0.04 0.26 6.71 0.00 0.00 0.00
D2 67 0.221 0.417 0.671 15.100 1.191 0.026 0.778 0.594 8.061 0.470 0.037 18.987 0.425 0.228 9.928 1.074 0.004 0.081 4.281 1.452 0.099 2.830 0.007 1.695 0.178 0.012 0.000 0.011 2.475 0.777 0.215 0.034 0.005 0.142 0.007 0.194 0.041 0.035 0.319 0.089 0.212 0.577 1.347 0.061 0.028 13.861 0.001 0.508 0.076 0.448 0.761 0.050 0.019 0.114 0.578 0.205 0.015 0.003 0.002 0.027 0.025 0.068 0.04 0.49 7.29 0.00 0.00 0.00
D2 69 0.133 0.374 0.969 15.670 0.868 0.012 0.532 0.671 8.790 0.445 0.017 23.895 0.618 0.378 9.267 1.350 0.002 0.090 4.548 1.913 0.117 3.324 0.005 1.698 0.118 0.006 0.001 0.007 3.054 0.972 0.172 0.044 0.014 0.103 0.012 0.296 0.064 0.035 0.483 0.157 0.320 0.664 1.303 0.047 0.013 8.382 0.000 0.451 0.079 0.583 0.875 0.052 0.019 0.128 0.644 0.183 0.007 0.009 0.001 0.036 0.014 0.064 0.03 0.28 4.60 0.00 0.00 0.00
D2 70 0.162 0.488 1.072 14.258 0.935 0.010 0.526 0.835 8.392 0.450 0.022 23.005 0.729 0.465 9.540 1.505 0.004 0.107 4.099 2.185 0.120 3.028 0.009 1.757 0.138 0.008 0.000 0.008 3.744 0.976 0.164 0.024 0.012 0.115 0.009 0.373 0.090 0.049 0.626 0.188 0.370 0.724 1.338 0.064 0.016 9.441 0.000 0.514 0.108 0.655 1.058 0.060 0.028 0.121 0.659 0.193 0.028 0.008 0.002 0.035 0.011 0.098 0.02 0.27 3.94 0.00 0.00 0.00
D2 71 0.416 2.726 1.564 15.489 0.641 0.022 0.196 0.155 3.719 2.208 0.022 11.412 0.338 0.255 6.582 0.377 0.004 0.114 4.613 3.524 0.036 2.641 0.004 1.907 0.158 0.003 0.000 0.008 0.958 2.026 0.114 0.143 0.009 0.134 0.016 0.172 0.040 0.025 0.386 0.074 0.317 0.415 0.596 0.016 0.012 16.076 0.001 0.652 0.339 0.749 1.224 0.034 0.010 0.463 0.296 0.133 0.009 0.001 0.001 0.007 0.009 0.149 0.01 2.65 12.59 0.00 0.00 0.00
D2 72 0.166 1.524 3.150 11.843 0.869 0.009 0.262 0.452 3.134 1.737 0.016 13.391 0.519 0.287 7.354 0.571 0.001 0.057 3.523 4.596 0.062 2.106 0.007 1.629 0.095 0.003 0.000 0.007 4.806 5.155 0.361 0.200 0.012 0.086 0.007 0.118 0.030 0.017 0.376 0.084 0.252 0.433 0.703 0.028 0.012 18.914 0.000 0.795 0.533 0.523 1.167 0.030 0.010 0.243 0.294 0.274 0.008 0.000 0.000 0.010 0.017 0.078 0.02 2.02 5.01 0.00 0.00 0.00
D2 73 0.378 0.458 0.936 6.149 8.225 0.021 3.346 5.753 1.501 0.591 0.027 4.309 4.760 1.387 5.145 4.406 0.050 0.510 1.925 0.506 3.019 1.406 0.007 0.927 0.316 0.013 0.004 0.036 1.319 1.796 1.264 0.139 0.024 0.443 0.024 0.506 0.269 0.077 0.424 0.338 0.145 0.974 0.671 14.299 1.749 1.782 0.000 1.718 0.043 0.676 1.033 1.085 2.423 0.328 0.547 0.627 2.741 0.948 0.037 2.227 0.016 0.074 1.30 0.23 1.31 0.08 0.17 0.04
D2 74 0.282 0.063 0.241 1.152 0.430 0.031 1.294 5.166 0.192 0.226 0.087 1.658 0.893 4.927 0.955 0.837 0.010 0.097 0.254 0.603 18.630 0.283 0.002 0.232 0.696 0.000 0.000 0.080 0.197 0.119 0.189 0.011 0.002 0.626 0.033 0.332 0.085 0.031 0.194 0.202 0.051 0.202 0.125 24.706 28.882 0.962 0.001 0.235 0.065 0.075 0.143 0.064 1.042 0.070 0.103 0.044 0.022 0.007 0.006 1.043 0.073 0.018 0.16 0.02 0.48 0.00 0.04 0.01
D2 77 0.043 0.010 0.077 0.370 0.200 0.017 0.222 1.314 0.065 0.026 0.023 0.936 0.369 2.502 0.480 0.563 0.003 0.007 0.043 0.029 7.798 0.174 0.005 0.030 0.056 0.000 0.000 0.005 0.071 0.070 0.034 0.001 0.000 0.045 0.001 0.047 0.020 0.004 0.036 0.043 0.004 0.017 0.046 35.326 45.106 0.124 0.000 0.084 0.004 0.014 0.079 0.011 1.775 0.044 0.106 0.032 0.012 0.007 0.001 1.184 0.014 0.002 0.11 0.00 0.11 0.00 0.04 0.00
D2 78 0.049 0.018 0.120 0.358 0.240 0.020 0.236 0.917 0.054 0.025 0.024 0.259 0.293 1.360 0.246 0.142 0.003 0.002 0.046 0.042 5.957 0.077 0.006 0.022 0.049 0.001 0.000 0.003 0.052 0.165 0.033 0.011 0.002 0.033 0.001 0.027 0.014 0.002 0.024 0.020 0.006 0.013 0.019 37.872 48.530 0.130 0.000 0.141 0.004 0.013 0.061 0.008 1.265 0.056 0.026 0.061 0.014 0.020 0.000 0.501 0.012 0.002 0.13 0.00 0.08 0.01 0.06 0.00
D2 79 0.035 0.004 0.075 0.338 0.202 0.011 0.158 2.673 0.056 0.009 0.012 0.782 0.803 2.424 0.337 0.416 0.001 0.004 0.027 0.017 8.850 0.150 0.005 0.018 0.018 0.000 0.000 0.001 0.098 0.105 0.028 0.003 0.000 0.012 0.001 0.040 0.012 0.001 0.056 0.061 0.005 0.012 0.024 31.776 47.206 0.152 0.000 0.077 0.002 0.012 0.031 0.013 1.077 0.018 0.058 0.052 0.015 0.012 0.000 1.189 0.006 0.000 0.23 0.00 0.15 0.01 0.03 0.00
D2 80 0.081 0.005 0.099 0.492 0.179 0.014 0.925 5.756 0.086 0.018 0.018 0.781 1.741 3.607 0.380 0.452 0.002 0.007 0.089 0.055 14.447 0.241 0.008 0.076 0.015 0.000 0.000 0.002 0.069 0.102 0.020 0.011 0.000 0.019 0.002 0.190 0.048 0.019 0.219 0.158 0.049 0.067 0.080 26.175 38.249 0.832 0.001 0.105 0.004 0.064 0.164 0.009 1.321 0.012 0.078 0.039 0.014 0.010 0.001 1.475 0.007 0.003 0.17 0.00 0.60 0.00 0.03 0.00
D2 81 0.027 0.005 0.084 0.410 0.090 0.002 0.184 1.041 0.188 0.010 0.004 0.454 0.699 1.766 0.270 0.451 0.002 0.076 0.216 0.182 5.455 0.246 0.003 0.241 0.018 0.000 0.000 0.001 0.075 0.021 0.010 0.004 0.001 0.048 0.008 0.338 0.130 0.068 0.228 0.149 0.238 0.119 0.122 36.799 45.779 0.139 0.000 0.043 0.002 0.314 0.229 0.006 1.370 0.006 0.085 0.033 0.014 0.015 0.002 1.119 0.001 0.000 0.12 0.00 0.18 0.00 0.05 0.00
D2 84 0.045 0.004 0.454 0.430 0.086 0.190 0.064 1.722 0.061 0.012 0.166 0.424 0.674 2.564 0.166 0.179 0.025 0.049 0.094 0.274 7.242 0.075 0.030 0.052 0.028 0.192 0.074 0.034 0.519 0.044 0.047 0.005 0.002 0.051 0.032 0.398 0.134 0.068 0.131 0.196 0.103 0.033 0.034 35.444 44.323 0.154 0.027 0.072 0.006 0.055 0.280 0.006 0.950 0.003 0.027 0.016 0.019 0.018 0.016 0.886 0.014 0.004 0.23 0.00 0.07 0.11 0.06 0.00
D2 85 0.079 0.010 0.755 0.369 0.107 0.485 0.083 4.197 0.041 0.009 0.451 0.927 1.125 1.274 0.379 0.510 0.039 0.056 0.055 0.291 6.308 0.087 0.343 0.034 0.025 0.658 0.404 0.087 0.780 0.134 0.095 0.007 0.002 0.058 0.034 0.459 0.161 0.124 0.115 0.193 0.190 0.021 0.027 32.707 42.596 0.262 0.066 0.263 0.005 0.052 0.243 0.005 0.550 0.005 0.019 0.035 0.021 0.014 0.035 0.486 0.015 0.008 0.70 0.00 0.09 0.11 0.10 0.02
D2 86 0.070 0.009 1.053 0.284 0.083 0.484 0.024 2.717 0.029 0.017 0.299 1.540 1.032 1.377 0.385 0.823 0.063 0.069 0.034 0.331 4.526 0.074 1.031 0.025 0.029 1.932 0.679 0.130 1.271 0.571 0.101 0.022 0.003 0.135 0.056 0.893 0.331 0.211 0.102 0.419 0.330 0.015 0.023 31.850 40.630 0.268 0.109 0.207 0.004 0.050 0.102 0.013 0.808 0.006 0.029 0.030 0.007 0.007 0.060 1.107 0.028 0.008 0.44 0.01 0.08 0.24 0.22 0.05
D2 87 0.079 0.011 2.813 0.290 0.147 0.588 0.036 4.789 0.033 0.025 0.536 1.411 1.855 0.925 0.560 0.771 0.087 0.085 0.037 0.505 4.441 0.079 2.858 0.018 0.079 2.237 1.011 0.189 1.608 4.383 0.303 0.221 0.002 0.168 0.087 0.904 0.319 0.211 0.157 0.418 0.410 0.022 0.039 28.384 30.862 0.476 0.149 0.379 0.018 0.066 0.099 0.007 0.509 0.007 0.021 0.210 0.031 0.023 0.068 0.834 0.035 0.015 1.36 0.01 0.07 0.30 0.27 0.06
D2 88 0.114 0.013 2.878 0.323 0.286 1.489 0.107 1.896 0.015 0.019 2.020 0.899 0.769 0.565 0.320 0.252 0.158 0.087 0.027 0.528 1.518 0.031 7.524 0.011 0.189 3.842 2.017 0.378 2.186 5.293 0.487 0.147 0.010 0.245 0.162 1.363 0.706 0.463 0.168 0.606 0.348 0.011 0.021 27.566 25.261 0.480 0.257 0.880 0.005 0.138 0.128 0.012 0.662 0.008 0.012 0.233 0.012 0.009 0.169 2.064 0.064 0.008 0.81 0.01 0.04 0.24 0.30 0.14
D2 90 0.195 0.025 4.571 0.507 0.426 0.866 0.177 4.020 0.101 0.046 0.972 3.164 1.780 1.285 1.440 1.604 0.569 0.364 0.141 0.610 2.209 0.263 3.595 0.052 0.256 1.104 0.796 0.945 3.426 12.616 1.000 1.082 0.012 0.954 0.669 3.523 1.602 1.020 0.404 1.091 0.995 0.058 0.093 16.116 11.082 1.109 0.205 0.514 0.076 0.334 0.199 0.017 1.508 0.013 0.057 1.157 0.028 0.011 0.544 3.580 0.121 0.021 0.50 0.01 0.24 0.69 0.92 0.32
D2 91 0.168 0.019 5.280 0.761 0.690 1.202 0.260 3.511 0.175 0.070 1.444 3.348 1.654 1.319 1.774 1.459 0.701 0.633 0.265 0.772 1.594 0.342 2.779 0.106 0.398 1.360 0.868 1.395 3.627 15.322 0.852 1.267 0.013 1.681 0.913 4.344 1.762 1.034 0.450 1.310 1.072 0.080 0.157 12.345 6.780 1.441 0.291 0.632 0.096 0.420 0.284 0.020 1.675 0.023 0.088 1.200 0.020 0.011 0.629 3.699 0.148 0.027 0.38 0.03 0.31 0.35 0.59 0.28

13
Table S7.5: Overview of relative cells abundance per subcommunity (%) in samples taken from reactor D3 (G1 to G68). Time: time points of sampling (day).
Reacto r Time G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 G13 G14 G15 G16 G17 G18 G19 G20 G21 G22 G23 G24 G25 G26 G27 G28 G29 G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G40 G41 G42 G43 G44 G45 G46 G47 G48 G49 G50 G51 G52 G53 G54 G55 G56 G57 G58 G59 G60 G61 G62 G63 G64 G65 G66 G67 G68
D3 0.25 0.003 0.005 0.070 0.024 0.228 0.003 0.054 0.099 0.008 0.003 0.001 0.051 1.751 2.399 0.022 0.042 0.153 0.452 0.006 0.015 0.426 0.005 0.005 0.012 0.024 0.001 0.000 0.054 0.055 0.064 0.040 0.015 0.006 0.190 0.209 47.003 13.521 7.572 9.457 9.950 2.822 0.046 0.015 0.739 0.120 0.021 0.001 0.045 0.003 0.096 0.011 0.012 0.348 0.004 0.002 0.258 0.093 0.144 0.006 0.763 0.004 0.001 0.04 0.00 0.01 0.30 0.10 0.00
D3 1 0.075 0.009 0.315 0.705 0.311 2.647 1.121 2.446 0.495 0.011 0.269 0.967 4.178 5.660 3.026 3.228 0.438 1.471 0.328 0.033 1.797 1.413 0.024 1.628 0.304 0.051 0.024 1.537 0.341 0.032 0.275 0.013 0.002 2.860 0.911 18.622 4.559 2.075 9.042 5.194 3.927 5.051 2.377 0.619 0.273 0.981 0.324 0.097 0.004 2.360 0.526 0.092 0.901 0.007 0.680 0.123 0.721 0.210 0.136 0.424 0.597 0.001 0.17 0.00 0.38 0.30 0.25 0.03
D3 2 0.076 0.026 1.748 1.496 1.331 7.742 1.420 1.914 1.293 0.073 0.388 0.827 3.117 3.329 11.800 7.730 0.407 1.157 2.299 0.379 0.511 6.888 0.034 2.032 0.174 0.480 0.441 1.236 0.378 0.209 0.284 0.017 0.004 2.015 0.734 5.188 1.724 0.949 4.492 1.246 1.666 4.907 3.858 0.383 0.487 0.654 1.270 0.870 0.016 1.956 1.054 0.169 0.271 0.022 1.678 0.156 0.403 0.082 0.244 0.234 0.622 0.009 0.43 0.01 0.23 0.32 0.34 0.08
D3 3 0.273 0.033 4.781 2.961 3.991 5.499 2.660 3.292 1.026 0.254 0.535 0.944 2.720 2.913 14.042 6.397 0.449 1.344 3.153 1.770 0.327 7.032 0.072 1.656 0.229 0.972 0.351 1.127 0.292 0.621 0.306 0.043 0.069 1.411 0.601 1.565 0.604 0.369 2.266 0.490 1.067 3.255 3.193 1.528 1.102 0.969 0.753 1.127 0.029 1.223 2.316 0.146 0.301 0.058 0.879 0.429 0.075 0.018 0.249 0.265 0.562 0.027 0.16 0.03 0.22 0.19 0.32 0.07
D3 5 0.039 0.027 0.860 0.410 0.817 0.164 0.716 10.351 0.216 0.084 0.049 0.752 6.490 2.639 0.786 1.314 0.031 0.102 0.247 0.289 8.111 0.343 0.086 2.229 0.022 0.301 0.086 0.067 0.706 0.101 0.176 0.012 0.015 0.767 0.038 0.350 0.106 0.068 0.336 0.280 0.133 0.488 0.465 24.805 20.459 0.459 0.037 0.181 0.020 1.028 0.216 0.053 3.175 0.039 0.077 0.141 0.198 0.127 0.025 3.433 0.019 0.003 3.62 0.01 0.07 0.06 0.06 0.02
D3 7 0.120 0.861 7.013 1.980 0.141 1.016 0.249 8.312 0.928 0.508 0.122 8.093 2.382 2.412 2.794 5.137 0.244 0.971 0.510 2.997 1.829 0.279 0.078 0.737 0.050 0.379 0.264 2.033 23.348 0.533 5.685 0.256 0.038 0.332 0.593 0.522 0.209 0.140 0.663 0.265 0.276 0.459 0.834 1.186 1.225 3.619 0.335 0.274 2.325 0.821 0.374 0.016 0.275 0.561 0.156 0.044 0.040 0.018 0.401 0.240 0.566 0.018 0.25 0.03 0.14 0.21 0.21 0.07
D3 8 0.209 0.363 7.263 2.097 0.140 2.964 1.756 9.402 0.228 0.723 0.742 4.362 2.131 1.567 1.239 2.677 0.126 0.529 0.275 3.965 1.946 0.176 0.106 0.445 0.080 1.484 0.704 0.374 32.441 0.189 4.710 0.031 0.052 0.372 0.177 0.476 0.107 0.060 0.850 0.202 0.383 0.421 0.517 0.821 0.368 1.992 0.418 0.391 2.454 0.313 0.290 0.017 0.176 0.505 0.068 0.036 0.026 0.017 0.083 0.234 0.146 0.022 2.27 0.02 0.15 0.03 0.07 0.02
D3 9 0.503 0.330 4.694 1.403 0.245 0.958 0.665 10.187 1.152 0.750 0.532 3.266 2.291 0.918 1.211 1.688 0.062 0.195 0.298 2.324 1.587 0.204 0.092 0.978 0.297 0.547 0.256 0.244 36.225 0.523 11.563 0.026 0.109 0.446 0.095 0.269 0.057 0.030 0.605 0.140 0.274 0.381 1.115 0.646 0.336 1.239 0.103 0.568 1.503 0.549 0.182 0.029 0.148 0.357 0.174 0.106 0.016 0.014 0.037 0.110 0.122 0.068 3.63 0.07 0.06 0.09 0.09 0.01
D3 10 1.203 0.599 4.746 2.145 0.509 0.593 0.463 9.017 4.056 1.104 0.427 3.711 4.836 2.320 1.040 1.452 0.192 1.262 0.539 3.506 1.520 0.426 0.311 3.589 1.047 0.329 0.074 0.524 16.665 0.310 4.571 0.100 1.076 1.885 0.333 0.507 0.122 0.076 1.402 0.283 0.839 2.967 3.859 0.810 0.552 0.803 0.075 0.395 1.554 4.017 0.394 0.025 0.128 0.416 0.498 0.120 0.025 0.006 0.103 0.174 0.290 0.337 1.96 0.20 0.08 0.22 0.24 0.04
D3 11 0.771 1.240 3.799 3.381 3.412 0.200 1.790 2.193 2.726 0.727 0.095 1.054 2.665 1.039 0.797 0.621 0.050 0.322 0.700 2.161 2.566 0.429 0.112 1.440 0.579 0.061 0.008 0.268 1.331 5.002 0.795 0.207 0.412 1.033 0.123 0.354 0.077 0.050 0.493 0.243 0.280 0.765 1.924 4.479 21.931 1.513 0.020 2.387 0.232 1.415 0.949 0.117 0.367 0.161 0.480 1.498 0.048 0.098 0.060 0.417 0.107 0.344 14.36 0.34 0.26 0.03 0.08 0.01
D3 12 0.174 0.095 5.387 2.901 6.604 0.037 2.634 1.224 7.018 0.118 0.013 1.086 1.920 0.828 1.141 0.846 0.008 0.109 0.739 0.969 2.367 0.822 0.014 1.496 0.107 0.008 0.003 0.038 0.882 8.781 0.580 0.308 0.022 0.343 0.027 0.155 0.034 0.010 0.455 0.117 0.160 0.691 3.086 6.007 11.461 1.608 0.003 5.552 0.019 2.184 2.508 0.185 0.556 0.033 1.372 1.968 0.081 0.093 0.004 0.324 0.009 0.020 11.09 0.05 0.51 0.00 0.01 0.00
D3 14 0.023 0.022 3.619 1.946 13.291 0.003 6.494 0.954 3.526 0.078 0.006 1.428 3.704 0.642 1.452 4.324 0.001 0.048 0.716 0.420 0.863 1.368 0.000 0.314 0.063 0.001 0.000 0.016 0.659 8.356 0.458 0.122 0.001 0.083 0.008 0.145 0.023 0.011 2.104 0.120 0.169 0.440 2.577 9.754 6.261 3.291 0.002 6.414 0.010 1.787 2.487 0.214 1.051 0.023 2.440 1.790 0.059 0.050 0.001 0.698 0.012 0.010 2.07 0.03 0.94 0.00 0.00 0.00
D3 15 0.090 0.189 6.030 3.069 9.545 0.082 5.950 0.834 2.736 0.198 0.068 1.139 3.455 0.639 1.371 1.892 0.002 0.049 1.061 0.884 0.947 0.691 0.008 0.451 0.112 0.002 0.000 0.022 0.552 10.500 0.410 0.752 0.000 0.098 0.010 0.213 0.033 0.014 2.303 0.165 0.266 0.382 2.524 9.779 6.025 3.013 0.002 8.461 0.152 1.433 3.453 0.168 1.169 0.095 1.329 1.819 0.054 0.042 0.000 0.457 0.047 0.018 1.56 0.07 1.12 0.00 0.01 0.00
D3 17 0.907 0.049 4.686 2.627 9.712 0.778 4.613 2.044 1.792 0.392 0.670 2.623 3.415 0.889 3.488 2.494 0.004 0.129 3.593 0.667 0.717 1.291 0.030 1.087 0.623 0.000 0.000 0.058 1.106 9.741 0.439 0.464 0.000 0.270 0.028 0.226 0.036 0.015 1.366 0.187 0.219 0.537 1.362 11.603 3.860 4.149 0.009 5.400 0.050 0.632 2.445 0.105 1.561 0.026 0.552 1.689 0.028 0.015 0.000 0.396 0.672 0.006 0.38 0.04 1.00 0.00 0.01 0.00
D3 18 1.814 0.075 3.518 2.819 8.670 0.858 5.497 1.993 1.709 0.571 0.374 3.011 3.182 1.060 4.898 2.569 0.005 0.049 4.916 0.655 0.770 1.728 0.043 1.358 0.772 0.000 0.002 0.016 1.888 7.140 0.786 0.606 0.000 0.516 0.014 0.179 0.031 0.017 1.118 0.195 0.163 0.591 1.059 9.974 4.078 5.437 0.001 4.706 0.045 0.424 2.411 0.145 1.470 0.042 0.575 0.852 0.026 0.011 0.002 0.395 0.784 0.008 0.24 0.04 1.09 0.00 0.01 0.00
D3 19 1.200 0.204 4.248 2.500 7.092 1.172 5.591 1.966 1.856 1.243 0.538 2.475 2.545 1.222 3.450 1.989 0.008 0.243 7.376 0.805 0.929 1.258 0.089 1.616 0.653 0.003 0.004 0.105 1.565 7.038 0.591 1.726 0.002 0.368 0.053 0.403 0.082 0.027 0.874 0.330 0.306 0.488 0.781 10.316 4.140 4.740 0.014 5.263 0.087 0.618 2.299 0.106 1.394 0.129 0.395 1.155 0.023 0.013 0.003 0.600 0.462 0.053 0.18 0.12 0.87 0.00 0.00 0.00
D3 21 1.042 0.771 3.679 2.453 4.751 1.117 4.280 2.330 3.301 0.934 0.701 3.019 2.703 1.525 4.369 2.357 0.005 0.161 7.854 0.968 0.791 1.528 0.039 2.118 1.115 0.001 0.000 0.083 1.886 5.690 0.334 0.678 0.001 0.407 0.030 0.507 0.111 0.040 0.951 0.451 0.253 0.582 0.874 9.934 3.066 4.939 0.013 4.841 0.280 0.599 2.444 0.073 2.388 0.147 0.464 0.270 0.026 0.022 0.002 0.872 1.115 0.064 0.09 0.26 1.30 0.00 0.01 0.00
D3 22 0.582 0.769 3.674 2.715 5.073 0.648 3.695 2.032 2.893 1.394 0.277 2.393 2.530 1.538 3.697 1.938 0.032 0.436 10.000 1.073 0.824 1.423 0.026 2.313 0.787 0.001 0.006 0.193 1.501 7.560 0.393 0.351 0.002 0.495 0.122 0.704 0.164 0.067 1.033 0.471 0.435 0.673 0.873 9.953 3.074 5.354 0.014 3.952 0.121 0.838 2.173 0.086 1.698 0.212 0.420 1.193 0.035 0.038 0.007 0.778 0.721 0.076 0.10 0.29 1.05 0.00 0.01 0.00
D3 23 0.773 0.949 3.028 4.140 5.851 0.588 3.261 2.134 3.045 1.535 0.191 2.927 2.607 1.575 3.936 2.094 0.013 0.327 7.858 0.861 0.803 1.600 0.006 2.525 0.797 0.000 0.000 0.124 1.850 7.279 0.619 0.203 0.001 0.652 0.068 0.536 0.104 0.047 1.315 0.411 0.526 0.958 1.249 9.420 3.586 4.830 0.006 3.998 0.035 1.037 1.718 0.110 1.170 0.298 0.564 0.661 0.038 0.018 0.003 0.481 0.961 0.116 0.12 0.60 0.85 0.00 0.01 0.00
D3 24 0.751 1.885 2.916 6.902 4.033 0.178 2.948 1.416 4.526 2.441 0.087 2.001 2.257 1.435 2.913 1.761 0.028 0.460 7.834 1.218 0.572 1.460 0.003 3.136 0.819 0.000 0.002 0.165 1.304 6.878 0.232 0.261 0.001 0.534 0.146 0.775 0.163 0.085 1.530 0.474 0.965 1.145 1.265 8.821 2.798 3.560 0.006 4.435 0.032 1.694 1.681 0.078 1.669 0.557 0.551 0.364 0.039 0.019 0.006 0.541 0.306 0.255 0.07 1.72 0.87 0.01 0.01 0.00
D3 25 1.010 2.461 1.985 9.745 3.781 0.152 2.680 1.164 5.697 2.809 0.059 1.663 2.041 1.492 2.832 1.709 0.033 0.507 7.885 1.110 0.526 1.597 0.001 3.724 0.887 0.000 0.001 0.138 1.063 4.788 0.260 0.157 0.000 0.649 0.120 0.743 0.138 0.054 1.623 0.464 1.094 1.302 1.368 7.836 2.572 3.370 0.008 3.478 0.024 2.110 1.392 0.091 1.488 0.795 0.573 0.245 0.033 0.008 0.004 0.435 0.289 0.296 0.05 2.62 0.74 0.00 0.02 0.00
D3 28 0.616 3.475 1.368 20.107 3.295 0.116 1.725 0.875 9.026 3.234 0.049 1.409 1.556 0.879 2.963 1.274 0.010 0.202 7.688 2.964 0.292 1.744 0.004 3.492 0.624 0.000 0.000 0.038 1.209 2.761 0.364 0.134 0.000 0.426 0.025 0.281 0.037 0.020 0.991 0.194 0.599 1.148 1.855 4.199 2.364 3.106 0.001 1.994 0.042 1.733 0.814 0.094 0.305 0.603 0.806 0.204 0.040 0.014 0.002 0.109 0.204 0.221 0.05 3.48 0.54 0.01 0.00 0.00
D3 29 1.366 7.406 2.403 19.042 2.843 0.150 1.270 0.557 3.241 8.931 0.120 1.160 0.601 0.424 2.649 0.605 0.009 0.177 5.498 6.421 0.334 1.083 0.030 2.353 0.621 0.003 0.002 0.046 0.859 3.024 0.429 0.261 0.006 0.374 0.037 0.132 0.018 0.012 0.333 0.114 0.237 0.416 0.703 3.275 2.689 3.453 0.005 1.730 0.138 0.949 0.975 0.082 0.215 0.818 0.303 0.259 0.032 0.033 0.005 0.066 0.038 1.399 0.09 6.69 0.44 0.00 0.01 0.00
D3 30 3.061 6.947 3.293 13.265 1.732 0.294 1.012 0.459 4.026 7.492 0.243 0.870 0.644 0.654 3.041 0.529 0.048 0.502 5.599 3.789 0.315 1.313 0.043 3.845 1.894 0.006 0.003 0.219 0.539 5.113 0.265 0.317 0.002 1.313 0.158 0.248 0.045 0.017 0.500 0.195 0.328 0.452 0.718 3.104 2.144 2.892 0.012 1.587 0.101 1.611 0.929 0.062 0.316 1.060 0.314 0.382 0.011 0.006 0.015 0.054 0.110 2.494 0.04 6.75 0.61 0.02 0.02 0.01
D3 31 4.324 0.629 4.537 5.396 4.078 1.232 1.504 1.079 0.807 2.042 0.403 0.640 0.416 1.729 1.168 0.172 0.123 0.210 2.021 0.923 4.558 0.380 0.239 0.883 1.024 0.009 0.013 0.558 0.227 5.945 0.069 0.079 0.000 1.436 0.216 0.239 0.058 0.028 0.327 0.199 0.110 0.037 0.081 12.901 19.171 2.661 0.062 4.469 0.012 0.197 1.697 0.023 0.396 0.185 0.033 0.727 0.017 0.007 0.100 0.272 0.341 0.146 0.08 1.75 4.45 0.03 0.09 0.02
D3 35 2.568 0.133 9.057 1.012 0.396 2.072 0.155 1.346 6.608 0.350 0.726 0.482 1.391 2.799 0.913 0.530 0.074 0.187 0.629 2.011 3.901 0.165 2.533 1.780 1.003 0.237 0.147 0.720 0.065 19.652 0.015 1.337 0.001 0.636 0.149 0.352 0.054 0.031 0.308 0.347 0.153 0.017 0.378 13.814 13.132 0.118 0.360 1.136 0.118 0.724 0.103 0.007 0.806 0.027 0.197 0.213 0.025 0.016 0.137 0.065 0.365 0.059 0.08 0.81 0.09 0.02 0.07 0.09
D3 36 1.697 0.130 9.625 1.072 0.271 1.383 0.120 0.986 6.996 0.282 0.614 0.480 1.076 1.508 0.683 0.548 0.066 0.171 0.408 2.358 2.508 0.113 1.606 2.042 0.820 0.121 0.084 0.536 0.089 17.638 0.020 1.275 0.001 0.832 0.130 0.488 0.067 0.024 0.237 0.507 0.117 0.016 0.334 17.928 15.423 0.091 0.198 1.210 0.111 0.748 0.123 0.006 2.098 0.029 0.166 0.152 0.018 0.020 0.086 0.201 0.317 0.037 0.06 0.75 0.05 0.01 0.05 0.04
D3 37 0.703 0.051 5.461 0.584 0.567 0.307 0.338 2.063 2.880 0.122 0.174 0.691 2.173 9.155 0.733 0.780 0.021 0.058 0.199 1.581 10.309 0.121 0.425 0.873 0.442 0.049 0.018 0.088 0.242 4.438 0.136 0.224 0.004 0.338 0.042 0.385 0.082 0.033 0.346 0.370 0.120 0.104 0.222 23.209 18.832 0.230 0.017 1.831 0.029 0.247 0.123 0.054 5.705 0.020 0.072 0.133 0.033 0.014 0.013 0.518 0.059 0.063 0.08 0.55 0.08 0.01 0.02 0.01
D3 38 0.034 0.003 0.104 0.136 0.215 0.012 0.245 0.941 0.067 0.009 0.013 0.360 0.662 9.868 0.181 0.157 0.002 0.008 0.045 0.038 13.957 0.028 0.004 0.035 0.040 0.000 0.000 0.005 0.168 0.021 0.058 0.007 0.000 0.028 0.009 0.084 0.029 0.010 0.167 0.089 0.021 0.013 0.040 33.701 28.741 0.422 0.001 0.022 0.001 0.031 0.095 0.032 7.970 0.004 0.015 0.031 0.095 0.069 0.002 0.534 0.018 0.005 0.08 0.01 0.20 0.00 0.01 0.00
D3 39 0.015 0.004 0.124 0.215 0.272 0.006 1.548 0.575 0.027 0.012 0.003 0.599 0.264 4.742 0.235 0.176 0.003 0.003 0.027 0.044 7.469 0.029 0.004 0.018 0.011 0.001 0.000 0.001 0.276 0.030 0.061 0.003 0.000 0.010 0.001 0.052 0.015 0.004 0.084 0.065 0.003 0.007 0.043 37.765 32.035 1.674 0.000 0.048 0.005 0.008 0.112 0.025 9.473 0.003 0.021 0.024 0.070 0.017 0.001 0.766 0.005 0.003 0.08 0.01 0.75 0.00 0.01 0.00
D3 43 0.150 0.003 0.143 0.222 0.383 0.050 0.688 1.369 0.029 0.005 0.009 1.314 0.725 8.177 0.333 0.535 0.198 0.040 0.028 0.037 10.859 0.096 0.032 0.021 0.009 0.005 0.001 0.009 0.683 0.077 0.087 0.013 0.012 0.019 0.026 0.245 0.052 0.022 0.140 0.176 0.043 0.018 0.042 36.088 22.492 1.107 0.002 0.206 0.003 0.014 0.321 0.023 8.022 0.004 0.022 0.043 0.039 0.019 0.458 0.711 0.003 0.006 0.07 0.01 0.39 1.04 1.62 0.16
D3 44 0.122 0.011 0.751 0.521 0.459 0.076 0.956 1.815 0.046 0.016 0.015 3.070 0.619 3.470 0.667 1.017 0.161 0.034 0.056 0.110 5.086 0.153 0.029 0.031 0.008 0.019 0.009 0.011 1.592 0.106 0.138 0.014 0.018 0.022 0.019 0.231 0.050 0.024 0.179 0.190 0.073 0.032 0.066 35.162 24.416 2.115 0.006 0.650 0.005 0.020 0.989 0.024 7.563 0.008 0.042 0.042 0.093 0.090 0.628 0.600 0.007 0.009 0.11 0.01 0.74 1.56 2.32 0.70
D3 45 0.088 0.007 0.806 0.434 0.586 0.059 0.558 2.117 0.050 0.020 0.015 4.624 0.385 1.085 0.559 1.216 0.189 0.031 0.079 0.070 1.473 0.081 0.020 0.031 0.014 0.009 0.007 0.017 2.349 0.126 0.185 0.014 0.012 0.030 0.026 0.348 0.075 0.041 0.112 0.243 0.104 0.019 0.036 40.994 19.267 2.406 0.007 0.496 0.004 0.022 0.648 0.022 8.835 0.005 0.024 0.042 0.028 0.018 1.226 1.453 0.014 0.007 0.17 0.01 0.73 1.61 2.26 1.35
D3 46 0.213 0.020 1.372 0.759 0.352 0.146 0.535 2.061 0.125 0.059 0.033 4.132 0.430 4.388 0.557 1.098 0.143 0.024 0.188 0.228 2.612 0.107 0.037 0.085 0.024 0.013 0.015 0.044 2.780 0.137 0.300 0.015 0.023 0.063 0.042 0.245 0.046 0.034 0.129 0.204 0.102 0.033 0.054 38.595 9.921 2.765 0.009 0.201 0.013 0.061 0.606 0.023 14.853 0.010 0.036 0.047 0.047 0.053 1.051 2.388 0.019 0.021 0.25 0.02 0.83 0.38 2.05 1.73
D3 49 0.025 0.005 0.514 0.427 0.157 0.015 0.034 0.952 0.025 0.037 0.002 2.022 0.443 12.588 0.366 0.647 0.005 0.002 0.058 0.209 11.604 0.090 0.005 0.014 0.004 0.002 0.002 0.002 0.495 0.015 0.030 0.003 0.006 0.005 0.005 0.117 0.019 0.005 0.136 0.156 0.005 0.017 0.034 32.400 17.773 0.530 0.002 0.020 0.013 0.013 0.260 0.005 14.568 0.009 0.038 0.022 0.028 0.037 0.023 2.406 0.002 0.003 0.09 0.00 0.37 0.03 0.04 0.02
D3 50 0.037 0.005 0.241 0.516 0.321 0.017 0.115 1.536 0.032 0.018 0.003 3.810 0.345 5.069 0.532 1.035 0.003 0.007 0.167 0.255 5.922 0.088 0.001 0.012 0.003 0.001 0.002 0.010 0.676 0.023 0.032 0.003 0.000 0.015 0.001 0.044 0.010 0.002 0.065 0.068 0.003 0.016 0.058 38.481 26.232 0.725 0.002 0.057 0.012 0.012 0.501 0.019 11.355 0.003 0.048 0.017 0.133 0.128 0.002 0.513 0.006 0.003 0.13 0.00 0.48 0.00 0.01 0.00
D3 51 0.057 0.008 0.253 0.627 0.309 0.039 0.547 2.503 0.059 0.030 0.018 7.077 0.385 1.194 0.961 1.837 0.005 0.014 0.157 0.141 2.950 0.080 0.003 0.054 0.019 0.001 0.001 0.018 1.185 0.044 0.044 0.003 0.001 0.028 0.007 0.074 0.021 0.012 0.063 0.057 0.010 0.034 0.123 37.634 29.163 1.194 0.004 0.202 0.008 0.026 0.637 0.018 8.143 0.004 0.042 0.035 0.069 0.100 0.004 0.565 0.017 0.008 0.30 0.01 0.74 0.00 0.01 0.00
D3 52 0.066 0.010 5.800 0.963 0.320 0.045 0.263 2.745 0.104 0.025 0.027 7.078 0.698 5.023 1.618 2.354 0.002 0.019 0.167 1.845 4.608 0.222 0.008 0.124 0.023 0.004 0.001 0.012 0.819 6.094 0.048 0.077 0.001 0.029 0.008 0.160 0.036 0.015 0.244 0.157 0.039 0.154 0.325 25.194 18.511 0.966 0.006 2.530 0.007 0.050 0.700 0.016 7.331 0.007 0.196 0.032 0.049 0.036 0.002 0.578 0.014 0.003 0.14 0.01 1.23 0.00 0.01 0.00
D3 53 0.221 0.040 10.383 4.191 3.229 0.116 1.833 2.654 0.202 0.416 0.076 3.703 0.476 0.673 4.487 1.239 0.004 0.027 0.522 6.764 0.835 0.344 0.012 0.392 0.078 0.012 0.008 0.025 0.655 5.733 0.106 0.028 0.029 0.054 0.008 0.101 0.017 0.004 0.106 0.072 0.035 0.127 0.700 15.309 7.184 0.556 0.008 13.432 0.024 0.072 2.927 0.033 4.733 0.163 0.233 0.180 0.142 0.115 0.001 2.309 0.062 0.051 1.27 0.07 0.37 0.01 0.01 0.00
D3 56 0.138 0.452 6.782 4.454 3.578 0.063 4.093 5.365 0.654 0.500 0.018 2.987 1.526 0.267 17.884 3.019 0.007 0.047 1.999 1.462 0.516 1.470 0.015 1.181 0.097 0.004 0.001 0.024 0.328 2.480 0.261 1.113 0.005 0.113 0.011 0.070 0.021 0.011 0.113 0.039 0.035 0.690 2.566 9.871 6.832 2.814 0.001 2.755 0.189 0.209 2.529 0.155 1.874 0.193 0.676 0.774 0.191 0.132 0.000 0.815 0.128 0.038 0.61 0.22 2.52 0.00 0.01 0.00
D3 57 0.175 0.920 7.103 3.924 2.967 0.033 2.592 7.811 0.980 0.678 0.007 4.540 1.693 0.273 17.942 3.489 0.005 0.037 1.565 1.444 0.680 1.218 0.012 1.146 0.055 0.003 0.001 0.012 0.400 2.824 0.199 2.121 0.012 0.079 0.008 0.065 0.018 0.003 0.129 0.034 0.038 0.644 2.796 7.818 4.381 4.320 0.002 1.708 0.300 0.194 1.640 0.132 1.290 0.257 0.565 0.956 0.136 0.113 0.000 0.711 0.046 0.188 0.54 0.30 3.71 0.00 0.00 0.00
D3 58 0.252 1.297 9.550 4.717 1.738 0.024 1.491 4.233 1.169 0.794 0.014 5.931 1.623 0.337 17.997 3.460 0.005 0.041 2.438 1.608 0.690 2.988 0.014 1.682 0.093 0.002 0.000 0.024 0.209 3.365 0.116 2.669 0.017 0.089 0.013 0.045 0.009 0.003 0.165 0.032 0.055 0.927 2.509 4.935 2.156 3.634 0.000 1.202 0.445 0.256 0.944 0.083 1.433 0.307 0.875 0.927 0.117 0.118 0.002 0.380 0.045 0.184 0.22 0.51 6.79 0.00 0.00 0.00
D3 59 0.132 0.489 9.300 4.292 2.511 0.009 1.075 6.925 1.378 0.480 0.003 12.469 1.888 0.359 18.315 4.198 0.003 0.048 1.663 1.693 1.075 2.350 0.009 1.037 0.044 0.001 0.000 0.014 0.997 3.541 0.169 1.799 0.008 0.081 0.006 0.043 0.006 0.001 0.175 0.029 0.039 0.769 2.688 3.487 1.486 4.778 0.000 1.128 0.078 0.193 0.474 0.120 0.371 0.082 0.759 0.553 0.136 0.104 0.000 0.211 0.013 0.021 0.22 0.39 3.28 0.00 0.00 0.00
D3 60 0.143 0.297 8.335 4.366 1.877 0.007 0.850 6.246 1.577 0.394 0.004 19.710 1.832 0.334 18.091 4.057 0.001 0.048 1.776 1.509 0.875 2.634 0.008 1.034 0.041 0.001 0.000 0.009 0.933 2.656 0.108 1.220 0.010 0.046 0.006 0.044 0.010 0.003 0.163 0.029 0.046 0.942 2.509 1.963 0.743 4.541 0.000 0.924 0.064 0.210 0.412 0.081 0.322 0.084 0.766 0.333 0.124 0.087 0.003 0.165 0.011 0.013 0.14 0.30 3.92 0.00 0.00 0.00
D3 65 0.293 0.287 6.014 4.865 1.883 0.020 0.623 6.160 1.749 0.417 0.002 19.264 1.607 0.315 15.243 3.693 0.004 0.019 1.653 1.590 0.866 2.411 0.006 0.830 0.039 0.001 0.000 0.004 3.747 2.260 0.128 1.013 0.003 0.029 0.007 0.041 0.008 0.004 0.164 0.032 0.063 0.528 1.907 0.186 0.176 11.904 0.000 0.766 0.042 0.200 0.843 0.067 0.041 0.063 0.627 0.313 0.050 0.033 0.000 0.029 0.003 0.057 0.06 0.46 4.29 0.00 0.00 0.00
D3 66 0.294 0.176 6.686 5.069 2.331 0.012 0.811 5.617 1.750 0.281 0.002 20.333 1.194 0.225 12.541 2.274 0.005 0.020 1.691 1.639 0.428 1.965 0.003 0.747 0.049 0.001 0.000 0.004 4.762 2.810 0.165 1.048 0.005 0.047 0.005 0.040 0.009 0.004 0.158 0.027 0.077 0.407 1.556 0.111 0.122 14.076 0.000 0.939 0.029 0.208 1.221 0.069 0.026 0.051 0.470 0.382 0.045 0.027 0.001 0.018 0.001 0.080 0.04 0.31 4.50 0.00 0.00 0.00
D3 67 0.396 0.211 6.851 6.061 1.968 0.027 0.810 3.844 1.723 0.367 0.006 20.519 1.100 0.216 12.279 2.326 0.004 0.019 2.123 1.573 0.378 2.593 0.002 0.913 0.040 0.001 0.000 0.005 2.236 2.374 0.103 0.920 0.005 0.043 0.007 0.035 0.010 0.005 0.112 0.019 0.066 0.509 1.550 0.077 0.074 13.646 0.002 0.894 0.033 0.188 1.795 0.061 0.023 0.069 0.566 0.310 0.038 0.019 0.002 0.010 0.003 0.051 0.03 0.36 7.38 0.01 0.00 0.00
D3 69 0.231 0.154 5.444 8.023 2.049 0.015 0.988 3.539 2.094 0.201 0.001 21.765 1.057 0.232 11.108 2.219 0.003 0.024 2.645 1.687 0.382 2.479 0.000 0.678 0.033 0.001 0.000 0.002 3.486 2.123 0.122 0.643 0.003 0.035 0.004 0.038 0.008 0.004 0.169 0.034 0.065 0.460 1.217 0.053 0.062 13.235 0.001 0.913 0.018 0.190 2.808 0.055 0.017 0.052 0.509 0.267 0.021 0.017 0.001 0.006 0.002 0.031 0.03 0.22 6.03 0.00 0.00 0.00
D3 70 0.327 0.350 5.042 10.207 1.901 0.026 0.782 2.611 2.184 0.326 0.003 18.478 0.703 0.157 9.431 1.382 0.003 0.024 3.303 1.847 0.238 2.013 0.001 0.562 0.026 0.001 0.000 0.007 2.916 2.032 0.100 0.672 0.001 0.040 0.009 0.020 0.004 0.003 0.093 0.024 0.045 0.323 0.915 0.036 0.043 16.018 0.002 1.023 0.036 0.157 3.767 0.056 0.006 0.071 0.380 0.273 0.021 0.010 0.002 0.003 0.005 0.033 0.02 0.36 8.53 0.01 0.00 0.00
D3 71 0.492 1.990 3.324 11.351 1.066 0.017 0.258 0.319 2.346 1.336 0.006 6.844 0.239 0.068 8.220 0.346 0.004 0.024 4.496 2.056 0.049 2.338 0.000 1.131 0.073 0.000 0.000 0.010 0.412 3.467 0.047 0.436 0.000 0.047 0.008 0.026 0.003 0.003 0.075 0.014 0.045 0.173 0.495 0.008 0.013 19.940 0.000 0.797 0.056 0.200 5.219 0.039 0.004 0.231 0.286 0.488 0.015 0.004 0.004 0.002 0.001 0.186 0.01 1.30 17.52 0.01 0.01 0.00
D3 72 0.292 1.879 2.053 9.379 2.955 0.010 0.557 0.248 2.426 1.814 0.006 7.465 0.202 0.088 8.347 0.368 0.001 0.027 3.211 2.013 0.040 2.087 0.001 1.177 0.082 0.002 0.000 0.004 1.440 3.558 0.157 0.120 0.000 0.041 0.004 0.029 0.011 0.006 0.084 0.014 0.053 0.150 0.479 0.009 0.012 25.712 0.001 1.488 0.046 0.220 3.839 0.033 0.004 0.171 0.336 0.965 0.010 0.005 0.000 0.001 0.003 0.253 0.01 1.81 12.19 0.00 0.00 0.00
D3 73 1.034 3.262 2.019 11.296 1.939 0.036 0.230 0.119 2.936 1.336 0.024 7.138 0.146 0.067 6.925 0.261 0.003 0.033 4.257 1.488 0.032 2.214 0.007 1.733 0.149 0.001 0.001 0.013 0.392 4.865 0.044 0.211 0.001 0.074 0.010 0.021 0.006 0.004 0.086 0.012 0.060 0.159 0.374 0.009 0.007 18.523 0.002 2.036 0.039 0.277 3.454 0.031 0.003 0.727 0.251 0.562 0.009 0.002 0.001 0.011 0.008 0.883 0.00 1.50 16.64 0.00 0.01 0.00
D3 74 1.627 5.096 2.813 11.688 1.433 0.086 0.339 0.101 2.298 2.348 0.042 3.712 0.236 0.120 6.580 0.247 0.008 0.069 3.758 1.427 0.037 2.474 0.024 1.368 0.177 0.004 0.007 0.034 0.060 5.790 0.027 0.382 0.004 0.137 0.019 0.076 0.013 0.010 0.179 0.040 0.143 0.241 0.430 0.013 0.006 15.531 0.009 1.786 0.068 0.437 3.023 0.029 0.004 1.051 0.251 0.590 0.008 0.002 0.006 0.011 0.017 1.121 0.01 2.35 17.96 0.00 0.01 0.01
D3 77 2.187 10.653 4.718 10.239 1.105 0.227 0.251 0.147 3.970 3.111 0.171 7.218 0.454 0.276 5.603 0.423 0.045 0.313 2.335 2.012 0.049 2.332 0.059 2.283 0.550 0.010 0.004 0.150 0.238 7.402 0.023 1.170 0.006 0.579 0.111 0.170 0.035 0.026 0.409 0.084 0.376 0.569 0.727 0.023 0.009 7.107 0.020 1.621 0.336 1.355 0.628 0.015 0.010 2.061 0.372 1.155 0.011 0.004 0.023 0.023 0.076 2.907 0.01 2.74 6.62 0.01 0.03 0.01
D3 78 2.054 7.609 5.815 10.793 0.862 0.239 0.112 0.090 4.727 2.462 0.188 2.495 0.349 0.206 4.676 0.323 0.015 0.257 3.112 2.086 0.041 1.985 0.031 2.266 0.533 0.007 0.010 0.123 0.059 10.323 0.012 1.759 0.003 0.519 0.068 0.122 0.021 0.015 0.291 0.048 0.310 0.481 0.609 0.013 0.009 8.536 0.019 2.868 0.493 1.198 1.280 0.012 0.005 1.693 0.279 1.322 0.007 0.005 0.008 0.020 0.062 3.296 0.00 1.78 8.96 0.00 0.01 0.01
D3 79 1.076 4.035 5.200 12.002 0.818 0.163 0.205 0.120 5.770 2.269 0.134 0.975 0.349 0.168 3.915 0.446 0.018 0.254 4.137 2.360 0.065 1.858 0.023 2.689 0.608 0.015 0.004 0.115 0.043 11.832 0.016 1.896 0.006 0.560 0.063 0.086 0.013 0.009 0.225 0.029 0.236 0.499 0.674 0.020 0.010 15.298 0.011 1.459 0.485 1.204 1.710 0.007 0.005 0.819 0.365 1.319 0.000 0.001 0.002 0.012 0.051 1.769 0.00 1.39 8.07 0.00 0.01 0.00
D3 80 1.329 4.100 5.871 12.821 0.400 0.175 0.128 0.056 5.716 1.642 0.129 1.326 0.177 0.108 3.391 0.201 0.017 0.253 4.409 1.668 0.025 1.313 0.038 2.661 0.735 0.008 0.006 0.089 0.029 11.584 0.007 3.572 0.003 0.625 0.056 0.037 0.007 0.004 0.155 0.018 0.185 0.337 0.507 0.014 0.005 12.389 0.006 1.282 0.792 1.289 2.418 0.002 0.002 0.991 0.209 0.609 0.000 0.001 0.001 0.010 0.041 1.539 0.00 1.32 11.15 0.00 0.01 0.00
D3 81 0.924 3.295 4.581 14.100 0.481 0.136 0.138 0.057 5.854 1.917 0.101 1.438 0.248 0.137 3.410 0.269 0.012 0.182 4.205 2.023 0.025 1.314 0.028 2.330 0.621 0.004 0.000 0.064 0.048 9.392 0.017 3.458 0.003 0.443 0.039 0.041 0.007 0.004 0.177 0.030 0.170 0.363 0.524 0.013 0.008 17.339 0.005 0.461 0.686 1.034 2.400 0.004 0.004 0.830 0.249 0.797 0.001 0.001 0.003 0.007 0.039 1.344 0.00 1.23 10.92 0.00 0.01 0.00
D3 84 0.959 2.127 6.121 14.149 0.736 0.112 0.237 0.090 6.159 1.509 0.096 3.148 0.226 0.102 3.189 0.318 0.006 0.187 3.884 2.069 0.045 1.376 0.040 2.401 0.705 0.003 0.001 0.040 0.251 15.163 0.032 3.042 0.002 0.475 0.029 0.028 0.006 0.004 0.121 0.011 0.151 0.375 0.513 0.006 0.007 14.727 0.004 0.615 0.532 1.111 0.948 0.007 0.005 0.724 0.277 1.139 0.002 0.001 0.000 0.011 0.019 1.092 0.00 0.94 7.59 0.00 0.00 0.00
D3 85 0.830 1.941 6.554 13.520 0.680 0.081 0.364 0.212 6.501 1.684 0.069 2.492 0.351 0.128 3.473 0.561 0.008 0.187 4.088 2.165 0.075 1.663 0.016 2.842 0.683 0.002 0.000 0.044 0.335 18.541 0.042 3.525 0.002 0.495 0.031 0.028 0.003 0.001 0.169 0.015 0.165 0.505 0.692 0.012 0.006 11.507 0.002 0.661 0.532 1.274 0.884 0.008 0.004 0.650 0.398 1.452 0.001 0.001 0.001 0.015 0.020 1.006 0.00 0.98 4.81 0.00 0.01 0.00
D3 86 0.945 2.603 6.419 12.319 0.613 0.082 0.161 0.090 5.299 1.721 0.086 4.669 0.304 0.182 3.987 0.328 0.012 0.189 2.953 2.281 0.037 1.730 0.029 2.228 0.559 0.006 0.002 0.053 0.256 16.947 0.026 3.831 0.004 0.377 0.037 0.056 0.004 0.002 0.220 0.026 0.198 0.430 0.612 0.011 0.004 10.268 0.002 0.516 0.712 0.997 1.111 0.009 0.004 0.962 0.324 1.301 0.001 0.001 0.002 0.014 0.029 1.402 0.00 1.06 8.34 0.00 0.01 0.00
D3 87 2.997 2.419 3.766 7.827 8.813 0.476 0.436 4.053 2.728 0.507 0.157 5.671 2.070 0.848 3.969 1.156 0.059 0.265 2.260 0.937 1.810 1.130 0.076 2.557 0.674 0.015 0.008 0.084 1.510 5.899 0.716 3.304 0.019 1.054 0.079 0.150 0.046 0.028 0.647 0.614 0.227 0.502 0.749 2.794 0.787 2.905 0.010 0.288 0.530 0.910 0.927 0.701 2.461 1.004 0.416 1.339 2.182 1.057 0.029 0.936 0.112 0.879 1.15 0.35 4.49 0.07 0.34 0.02
D3 88 0.981 0.088 6.813 5.867 0.482 0.225 3.551 2.908 2.386 0.113 0.146 4.916 2.155 0.463 1.324 3.804 0.906 0.224 0.758 0.313 0.846 1.777 0.045 3.160 0.327 0.016 0.009 0.076 0.868 0.403 0.223 0.152 0.023 0.748 0.449 0.119 0.179 0.874 0.466 0.111 0.269 2.434 2.462 25.118 6.394 1.090 0.013 2.040 0.014 2.713 3.242 0.084 0.795 0.031 1.038 0.191 0.078 0.016 0.128 0.816 0.080 0.039 0.16 0.08 0.47 0.04 0.42 0.45
D3 90 0.552 0.610 7.078 9.988 0.830 0.180 1.115 0.928 4.544 0.943 0.145 2.487 0.799 0.376 7.143 2.082 0.025 0.118 4.746 1.982 0.062 4.712 0.133 2.533 0.245 0.006 0.001 0.032 0.337 13.232 0.117 2.962 0.007 0.245 0.029 0.134 0.037 0.017 0.470 0.061 0.322 0.831 1.288 4.765 0.029 5.292 0.004 2.382 0.636 1.161 3.515 0.007 0.068 0.240 0.892 0.855 0.001 0.005 0.001 0.168 0.019 0.161 0.01 0.43 4.85 0.00 0.02 0.00
D3 91 0.484 0.789 6.138 11.106 0.932 0.168 0.726 0.682 4.175 0.906 0.122 2.097 0.833 0.420 6.033 1.481 0.006 0.087 4.672 1.976 0.080 3.495 0.127 2.064 0.193 0.006 0.001 0.022 0.315 14.161 0.097 2.357 0.001 0.161 0.013 0.067 0.013 0.015 0.448 0.049 0.245 0.707 1.087 0.849 0.004 10.590 0.003 2.523 0.359 0.972 4.495 0.005 0.022 0.264 0.725 0.784 0.003 0.001 0.002 0.084 0.014 0.199 0.01 0.53 8.00 0.00 0.01 0.00

14
2. Data lists of abiotic and biotic standard parameters
Standard parameters were measured (Fig. S3.1, methods in S3) and used to calculate correlations to
subcommunities, which were Time: time points of sampling (day); T: cultivation temperature (°C);
OD: optical density measured in 600 nm with 5mm cuvette; EC: electrical conductivity (mS cm-1);
NH4: concentration of ammonium (mg N L-1); PHO: concentration of orthophosphate (mg P L-1);
CODt: total chemical oxygen demand (mg L-1) of supernatant and biomass; CODs: chemical oxygen
demand (mg L-1) of supernatant; CODb: chemical oxygen demand (mg L-1) of biomass, calculated by
CODt minus CODs. Overview of measurement results per each reactor (C1, C2, D1, D2 and D3) show
as Table S7.6-10. The parameter ‘number of cells’ was removed from the calculation of correlations,
as it measured less time points and represented comparable values to CODb (Fig. S3.1).

15
Table S7.6: Overview of parameters measurement from C1.
Reactor Time T OD pH EC NH4 PHO CODt CODs CODb
C1 0.25 30 0.3315
C1 1 30 0.5015
C1 2 30 0.378
C1 3 30 0.281 7.985 1.16 28 63.79 668 73 595
C1 5 30 0.3225
C1 7 30 0.2555 7.88 1.11 33.5 60.16 618 166 452
C1 8 30 0.2875
C1 9 30 0.335
C1 10 30 0.343 8.12 1.16 27 62.67 528 83 445
C1 11 30 0.3565
C1 12 30 0.3615
C1 14 30 0.355 8.02 1.18 29 63.23 616 58 558
C1 15 30 0.3765
C1 17 30 0.3845 8 1.11 25 61.4 696 59 637
C1 18 30 0.3495
C1 19 30 0.369 8.125 1.09 20 62.36 654 79 575
C1 21 30 0.391 8.055 1.06 17.5 64.35 684 109 575
C1 22 30 0.3985
C1 23 30 0.4035 8.02 1.07 16 63.98 688 87 601
C1 24 30 0.4165
C1 25 30 0.413 8.005 1.07 14 66.05 707 75 632
C1 28 30 0.427 8.06 1.04 11 61.46 703 84 619
C1 29 30 0.429
C1 30 30 0.427
C1 31 30 0.404 8 1.05 10 61.57 677 89 588
C1 35 30
C1 36 30 0.391 7.85 1.04 6 62.59 672 71 601
C1 37 30 0.385 7.83 1.05 6 61.92 670 81 589
C1 38 30 0.385
C1 39 30 0.373 7.855 1.04 6 61.54 640 89 551
C1 43 30 0.3895 7.745 1.02 4 66.02 686 80 606
C1 44 30 0.3775
C1 45 30 0.362
C1 46 30 0.3305 7.855 1.07 9.5 67.65 630 92 538
C1 49 30 0.3235 7.765 1.01 4 63.92 609 91 518
C1 50 30 0.3155
C1 51 30 0.3505
C1 52 30 0.3465 7.75 0.99 2 59.35 642 79 563
C1 53 30 0.3225
C1 56 30 0.3285 7.775 1.02 2 60.7 615 93 522
C1 57 30 0.3195
C1 58 30 0.3105 7.755 1.04 3 63.25 577 86 491
C1 59 30 0.3
C1 60 30 0.3075 7.555 1.04 3 63.2 587 105 482
C1 65 30 0.371 7.745 1.02 2 57.05 676 122 554
C1 66 30 0.366
C1 67 30 0.355 7.715 1.01 1.8 57.61 614 78 536
C1 69 30 0.358 7.815 1.01 1.6 56.82 774 127 647
C1 70 30 0.346 7.735 1.01 2 59.97 684 147 537
C1 71 30 0.3405
C1 72 30 0.351
C1 73 30 0.3455 7.76 0.995 1.9 59.19 745 221 524
C1 74 30 0.352
C1 77 30 0.354 7.73 1.01 1.8 58.62 776 234 542
C1 78 30 0.355
C1 79 30 0.343 7.765 1.02 1.9 62.14 710 177 533
C1 80 30 0.3435
C1 81 30 0.3385 7.76 1.01 1.6 58.96 750 216 534
C1 84 30 0.339 7.79 1 1.6 59.64 713 187 526
C1 85 30 0.3355
C1 86 30 0.33 7.69 1.01 1.8 59.3 705 199 506
C1 87 30 0.3315
C1 88 30 0.3135 7.7 1.09 2.4 59.64 627 135 492
C1 90 30 0.2945 7.75 1.03 1.8 60.67 702 251 451
C1 91 30 0.455

16
Table S7.7: Overview of parameters measurement from C2.
Reactor Time T OD pH EC NH4 PHO CODt CODs CODb
C2 0.25 30 0.382
C2 1 30 0.519
C2 2 30 0.3655
C2 3 30 0.272 8.07 1.2 29.5 64.06 604 92 512
C2 5 30 0.307
C2 7 30 0.346 7.92 1.13 28 60.3 679 188 491
C2 8 30 0.337
C2 9 30 0.2675
C2 10 30 0.2695 8.13 1.13 32.5 63.29 503 105 398
C2 11 30 0.2895
C2 12 30 0.309
C2 14 30 0.324 8.095 1.23 28.5 62.6 614 40 574
C2 15 30 0.3425
C2 17 30 0.344 8.08 1.13 25.5 63.61 652 32 620
C2 18 30 0.348
C2 19 30 0.34 8.13 1.12 24.5 62.87 627 37 590
C2 21 30 0.35 8.15 1.13 28 63.27 622 96 526
C2 22 30 0.349
C2 23 30 0.325 8.08 1.15 27.5 64.34 576 67 509
C2 24 30 0.3205
C2 25 30 0.3225 8.045 1.15 27.5 65.48 585 78 507
C2 28 30 0.3105 8.125 1.12 24 63.11 565 54 511
C2 29 30 0.315
C2 30 30 0.2995
C2 31 30 0.2935 8.16 1.16 28 62.34 553 71 482
C2 35 30
C2 36 30 0.3895 8.05 1.17 25 65.26 698 40 658
C2 37 30 0.353 8.01 1.15 23.5 63.36 647 51 596
C2 38 30 0.314
C2 39 30 0.2905 8.32 1.15 23 65.06 571 87 484
C2 43 30 0.3505 7.955 1.025 5 65.49 678 90 588
C2 44 30 0.3555
C2 45 30 0.3675
C2 46 30 0.351 7.79 1.05 4.5 65.3 675 96 579
C2 49 30 0.234 7.865 1.09 19 66.96 477 120 357
C2 50 30 0.2775
C2 51 30 0.3715
C2 52 30 0.362 7.905 1.05 14 63.41 639 215 424
C2 53 30 0.3625
C2 56 30 0.399 7.76 1.03 4 61.6 643 124 519
C2 57 30 0.41
C2 58 30 0.407 7.72 1.03 3 64.07 594 112 482
C2 59 30 0.408
C2 60 30 0.4145 7.705 1.02 2 63.49 609 117 492
C2 65 30 0.4115 7.76 0.99 2 56.03 630 96 534
C2 66 30 0.404
C2 67 30 0.385 7.695 1.005 1.2 57.27 614 102 512
C2 69 30 0.3525 7.74 1.01 1.2 61.1 665 136 529
C2 70 30 0.324 7.68 1 1.2 59.75 693 171 522
C2 71 30 0.301
C2 72 30 0.3075
C2 73 30 0.313 7.69 0.99 1.4 60.65 674 179 495
C2 74 30 0.3205
C2 77 30 0.356 7.66 1 1.7 59.64 682 169 513
C2 78 30 0.3535
C2 79 30 0.3495 7.705 1.01 1.7 62.83 738 236 502
C2 80 30 0.358
C2 81 30 0.3525 7.735 0.99 1.6 61.69 731 214 517
C2 84 30 0.3375 7.76 0.99 1.7 62.6 708 176 532
C2 85 30 0.322
C2 86 30 0.3235 7.66 1 2.6 58.85 721 230 491
C2 87 30 0.3145
C2 88 30 0.3025 7.84 1.08 4.6 59.98 644 173 471
C2 90 30 0.305 7.895 1.03 7.2 60.89 580 124 456
C2 91 30 0.297

17
Table S7.8: Overview of parameters measurement from D1.
Reactor Time T OD pH EC NH4 PHO CODt CODs CODb
D1 0.25 30 0.3645
D1 1 30 0.557
D1 2 30 0.415
D1 3 30 0.2855 8.125 1.23 30 63.52 522 79 443
D1 5 30 0.279
D1 7 30 0.264 8.06 1.11 30.5 60.3 645 182 463
D1 8 35 0.289
D1 9 35 0.2815
D1 10 35 0.281 8.1 1.15 37 65.77 520 106 414
D1 11 40 0.2935
D1 12 40 0.366
D1 14 40 0.3795 8.13 1.25 34.5 65.76 468 102 366
D1 15 40 0.335
D1 17 40 0.378 8.17 1.21 36 65.99 495 61 434
D1 18 40 0.3675
D1 19 40 0.3855 8.41 1.215 36.5 65.98 498 66 432
D1 21 40 0.4155 8.36 1.2 38 67.39 545 85 460
D1 22 40 0.4215
D1 23 40 0.418 8.31 1.21 34.5 67.03 548 64 484
D1 24 40 0.41
D1 25 40 0.39 8.36 1.21 35.5 69.07 548 66 482
D1 28 40 0.338 8.29 1.19 31 63.99 542 62 480
D1 29 35 0.315
D1 30 35 0.28
D1 31 35 0.268 8.23 1.2 33 63 530 77 453
D1 35 30
D1 36 30 0.356 8.11 1.135 23.5 65.75 632 57 575
D1 37 30 0.352 8.17 1.14 24.5 64.45 645 64 581
D1 38 30 0.3435
D1 39 30 0.334 8.155 1.15 27 63.98 577 71 506
D1 43 30 0.3425 8.165 1.14 27 68.26 607 76 531
D1 44 30 0.346
D1 45 30 0.3315
D1 46 30 0.319 8.07 1.18 30 68.34 611 112 499
D1 49 30 0.338 8.13 1.13 27 65.22 630 84 546
D1 50 35 0.334
D1 51 35 0.3135
D1 52 35 0.317 8.18 1.155 29.5 62.33 537 70 467
D1 53 40 0.3085
D1 56 40 0.3355 8.285 1.2 32.5 64.63 561 62 499
D1 57 40 0.3445
D1 58 40 0.3415 8.29 1.21 32 66.31 558 58 500
D1 59 40 0.3305
D1 60 40 0.3315 8.32 1.2 31.5 66.31 546 41 505
D1 65 40 0.332 8.335 1.16 30 59.41 592 47 545
D1 66 40 0.3435
D1 67 40 0.3445 8.35 1.17 30.5 63.13 598 62 536
D1 69 40 0.3505 8.365 1.17 30 62.9 634 96 538
D1 70 40 0.3445 8.305 1.16 31.5 64.37 764 210 554
D1 71 35 0.347
D1 72 35 0.3105
D1 73 35 0.314 8.23 1.135 28 62.56 645 130 515
D1 74 30 0.329
D1 77 30 0.353 8.215 1.13 24 63.01 684 118 566
D1 78 30 0.3605
D1 79 30 0.359 8.2 1.13 24.5 64.76 719 142 577
D1 80 30 0.3575
D1 81 30 0.354 8.155 1.13 26 64.3 755 174 581
D1 84 30 0.36 8.195 1.12 25 63.51 713 129 584
D1 85 30 0.3595
D1 86 30 0.364 8.08 1.12 24.5 61.8 682 119 563
D1 87 30 0.369
D1 88 30 0.3685 8.15 1.19 25.5 61.46 731 142 589
D1 90 30 0.362 8.21 1.14 26.5 61.8 644 88 556
D1 91 30 0.3525

18
Table S7.9: Overview of parameters measurement from D2.
Reactor Time T OD pH EC NH4 PHO CODt CODs CODb
D2 0.25 30 0.364
D2 1 30 0.534
D2 2 30 0.372
D2 3 30 0.2655 8.2 1.22 28 62.18 552 66 486
D2 5 30 0.311
D2 7 30 0.364 7.975 1.11 30.5 60.84 567 83 484
D2 8 35 0.2975
D2 9 35 0.3425
D2 10 35 0.3685 8.255 1.13 35.5 66.4 468 91 377
D2 11 40 0.3995
D2 12 40 0.397
D2 14 40 0.367 8.28 1.25 39 65.76 475 69 406
D2 15 40 0.368
D2 17 40 0.3585 8.2 1.2 36.5 65.99 504 42 462
D2 18 40 0.3435
D2 19 40 0.3335 8.38 1.2 35 66.32 526 54 472
D2 21 40 0.3395 8.44 1.17 33.5 66.68 550 90 460
D2 22 40 0.344
D2 23 40 0.3475 8.36 1.18 31 66.85 557 50 507
D2 24 40 0.35
D2 25 40 0.3495 8.19 1.2 32.5 68.22 677 75 602
D2 28 40 0.3435 8.31 1.17 29 63.88 573 80 493
D2 29 35 0.338
D2 30 35 0.32
D2 31 35 0.2455 8.27 1.22 36 63.33 553 198 355
D2 35 30
D2 36 30 0.2195 8.145 1.19 30.5 66.6 343 84 259
D2 37 30 0.3115 8.105 1.18 30.5 65.17 467 66 401
D2 38 30 0.353
D2 39 30 0.3135 8.065 1.17 29 65.67 468 92 376
D2 43 30 0.351 8.11 1.15 29 67.34 577 126 451
D2 44 30 0.3555
D2 45 30 0.3645
D2 46 30 0.3405 7.48 1.23 33 69.87 572 162 410
D2 49 30 0.3565 8.045 1.15 29.5 67.53 553 112 441
D2 50 35 0.357
D2 51 35 0.31
D2 52 35 0.2895 8.14 1.18 32.5 63.88 426 92 334
D2 53 40 0.3445
D2 56 40 0.326 8.265 1.2 33 65.64 476 74 402
D2 57 40 0.346
D2 58 40 0.3315 8.365 1.22 33 66.42 510 70 440
D2 59 40 0.3385
D2 60 40 0.3475 8.205 1.22 34.5 67.19 588 70 518
D2 65 40 0.306 8.42 1.18 34 60.54 532 63 469
D2 66 40 0.309
D2 67 40 0.3065 8.395 1.195 33.5 63.01 534 68 466
D2 69 40 0.3155 8.415 1.19 33 63.13 642 155 487
D2 70 40 0.3165 8.34 1.18 33.5 64.59 677 173 504
D2 71 35 0.316
D2 72 35 0.29
D2 73 35 0.052 8.295 1.23 43.5 64.37 282 175 107
D2 74 30 0.19
D2 77 30 0.3785 8.08 1.16 31.5 63.35 557 150 407
D2 78 30 0.3815
D2 79 30 0.36 8.11 1.165 31.5 65.78 553 160 393
D2 80 30 0.377
D2 81 30 0.3875 8.06 1.16 29.5 64.42 610 177 433
D2 84 30 0.387 8.015 1.13 24.5 63.51 633 194 439
D2 85 30 0.38
D2 86 30 0.391 7.95 1.12 20.5 61.92 820 346 474
D2 87 30 0.4035
D2 88 30 0.395 7.82 1.13 11.5 60.89 659 182 477
D2 90 30 0.3805 7.735 1.03 4 61.35 705 197 508
D2 91 30 0.369

19
Table S7.10: Overview of parameters measurement from D3.
Reactor Time T OD pH EC NH4 PHO CODt CODs CODb
D3 0.25 30 0.403
D3 1 30 0.477
D3 2 30 0.352
D3 3 30 0.267 8.15 1.24 29.5 63.66 620 100 520
D3 5 30 0.286
D3 7 30 0.308 7.935 1.11 35 60.84 595 121 474
D3 8 35 0.326
D3 9 35 0.332
D3 10 35 0.298 8.17 1.16 37 65.62 480 83 397
D3 11 40 0.308
D3 12 40 0.292
D3 14 40 0.37 8.23 1.23 34 65.6 502 115 387
D3 15 40 0.382
D3 17 40 0.327 8.225 1.2 36 65.99 508 60 448
D3 18 40 0.334
D3 19 40 0.343 8.405 1.18 29.5 66.49 516 54 462
D3 21 40 0.368 8.35 1.175 33.5 67.03 499 79 420
D3 22 40 0.365
D3 23 40 0.37 8.32 1.19 31 66.67 559 103 456
D3 24 40 0.373
D3 25 40 0.367 8.325 1.19 32.5 68.03 548 77 471
D3 28 40 0.377 8.27 1.17 29.5 63.99 553 98 455
D3 29 35 0.402
D3 30 35 0.317
D3 31 35 0.254 8.19 1.25 41.5 62.34 411 117 294
D3 35 30
D3 36 30 0.467 8.125 1.16 28.5 64.05 572 52 520
D3 37 30 0.42 8.07 1.19 30 65.76 536 59 477
D3 38 30 0.372
D3 39 30 0.348 8.13 1.195 32.5 65.67 423 57 366
D3 43 30 0.326 8.14 1.21 38.5 68.65 472 76 396
D3 44 30 0.346
D3 45 30 0.369
D3 46 30 0.374 8.09 1.22 35.5 66.82 582 93 489
D3 49 30 0.355 8.125 1.2 38 66.67 540 114 426
D3 50 35 0.356
D3 51 35 0.367
D3 52 35 0.379 8.215 1.22 40.5 62.81 489 77 412
D3 53 40 0.397
D3 56 40 0.387 8.295 1.23 38 65.08 531 70 461
D3 57 40 0.383
D3 58 40 0.37 8.375 1.22 34.5 66.52 562 69 493
D3 59 40 0.351
D3 60 40 0.348 8.325 1.2 32 66.41 588 62 526
D3 65 40 0.317 8.355 1.17 31.5 59.97 635 69 566
D3 66 40 0.304
D3 67 40 0.294 8.36 1.19 32.5 62.9 556 63 493
D3 69 40 0.304 8.425 1.18 32 63.35 621 134 487
D3 70 40 0.302 8.36 1.17 32 64.14 606 118 488
D3 71 35 0.31
D3 72 35 0.317
D3 73 35 0.308 8.215 1.13 28.5 61.44 634 96 538
D3 74 30 0.294
D3 77 30 0.256 8.135 1.16 33.5 62.9 608 164 444
D3 78 30 0.263
D3 79 30 0.272 8.165 1.175 33 63.85 629 144 485
D3 80 30 0.294
D3 81 30 0.303 8.115 1.15 28.5 62.37 671 119 552
D3 84 30 0.325 8.175 1.12 26.5 62.37 714 130 584
D3 85 30 0.324
D3 86 30 0.34 8.02 1.15 28 59.41 728 167 561
D3 87 30 0.195
D3 88 30 0.218 8.155 1.35 50 62.83 518 210 308
D3 90 30 0.402 8.21 1.24 43 62.6 748 228 520
D3 91 30 0.369

20
S8: Biostatistic evaluation using gate-based cytometric data and standard parameters

If not stated otherwise, all calculations and graphs were generated with R version 3.3.3 (R
Development Core Team, 2017) using the packages flowCyBar (Koch et al., 2013;
http://bioconductor.org/packages/flowCyBar/) and vegan (Oksanen et al., 2017) for dissimilarity
analysis as well as Hmisc (Harrell, 2017), psych (Revelle, 2017), and qgraph (Epskamp et al., 2017) for
correlation analysis and visualization.
1. Dissimilarity analysis
Several steps were undertaken to test the reactors’ microbial community data for their dissimilarity.
Step 1: All data in Tables S7.1-5 (one inoculum and 325 samples from reactors) were used for
analyzing community dissimilarity.
Step 2: According to Koch et al. (2013), dissimilarities between samples were calculated as Bray-
Curtis distance for all five reactors. The combined data were visualized separately in two nMDS plots
for the C1-2 and D1-3 reactor set ups, respectively (Fig. 1B and Fig. 3B) by using the vegan package
‘metaMDS’ function, with arguments: distance=’bray’, try=500, trymax=1000.
Step 3: The results of the dissimilarity analyses were tested for significance (by function ‘ANOSIM’ in
R package vegan). In the ANOSIM procedure, the rank order of dissimilarity values was used (Clarke,
1993; Buttigieg and Ramette, 2014). The mean rank of dissimilarities within sample groups was
compared with the mean rank of dissimilarities between sample groups, and statistic R-values and
significance p-values were determined. R-values are usually given in the range from 0 to 1, with
higher R-values suggesting higher differences between sample groups. R-values can also be negative,
but close to 0, when dissimilarities between and within groups were considered to be same in
average. P-values confirm whether the difference between sample groups (R-value) is significant
(with p-values < 0.05).
In this study, the significance of dissimilarity was tested pairwise for reactors C1, C2, D1, D2, and D3
(e.g. C1×C2 and C1×D1). For the adaptation phase (days 0-7) no significant differences were found for
any pair of two reactors, since all R-values were negative and showed p-values above 0.05 (Tab. S8.1).
That means the dissimilarities between sample groups (reactors) was lower in comparison to those of
the samples within each reactor which can be expected for an adaptation phase. Contrarily, samples
and sample groups from days 8 to 91 (continuous cultivation) showed significant difference between
reactors (p value < 0.05, Tab. S8.1). The differences between reactors D1 to D3 were smaller (R-
values of D1×D2: 0.176, D1×D3: 0.119 and D2×D3: 0.029) in comparison to those of the control
reactors (R-value of C1×C2: 0.317). Furthermore, differences between reactors from different
operations (e.g. C1×D1) were generally higher than differences between reactors from the identical

21
operation, with exception of C2×D3 with an R-value of 0.288. The findings confirm that the microbial
community structures between reactors D1-D3 were more similar then all other tested reactor pairs.

Table S8.1: ANOSIM outputs for the adaptation and continuous cultivation phases. Significance of dissimilarity
between reactors were tested pairwise.
Adaptation phase (0 – 7 d)
Reactor pairs C1×C2 C1×D1 C1×D2 C1×D3 C2×D1 C2×D2 C2×D3 D1×D2 D1×D3 D2×D3
R-value -0.122 -0.161 -0.063 -0.107 -0.098 -0.144 -0.141 -0.043 -0.061 -0.157
p-value 0.890 0.970 0.699 0.886 0.815 0.945 0.921 0.637 0.641 0.959
Continuous cultivation (8 – 91 d)
Reactor pairs C1×C2 C1×D1 C1×D2 C1×D3 C2×D1 C2×D2 C2×D3 D1×D2 D1×D3 D2×D3
R-value 0.317 0.590 0.502 0.428 0.489 0.400 0.288 0.176 0.119 0.029
p-value 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.033

2. Correlation analysis
Data from S7 (Tab. S7.1-10) were used for correlation analysis according to Günther et al. (2016) to
find dependencies between parameter groups in the five reactors.
Step 1: Cytometric cell based biotic data as well as abiotic parameters were involved to get
information on microbial community behaviour. For the cytometric data the relative cells
abundances per dominant subcommunities (abundance above the average 1.47%) were used.
Step 2: The data were subsampled into phases with equal sample numbers (10 successive time
points). 1st phase: this phase comprised the adaptation phase with 6 (fivefold working volume
exchange, 7 days) and 4 successive time points (days 8 - 11) when continuous growth conditions
were already established. 2nd phase: This phase comprised the majority of data. In reactors C1 and C2,
correlations were calculated following the height of drift events for 10 time points which were two
for C1: day 14 (drift 1) and day 46 (drift 2) and three for C2: day 9 (drift 1), day 49 (drift 2), and day
65 (drift 3). In the reactors D1-D3, the subsampling was performed according to the temperature
pressures which were set up at days 7 (T1), 28 (T2), 49 (T3) and 70 (T4). 3rd phase: The last phase
represented the communities in the reference spaces.
Step 3: The Spearman’s rank-order correlation coefficient (rho, range from -1 to 1) was used for
correlation of the cytometric cell data with abiotic and biotic parameters. Respective p-values were
also recorded.
Step 4: All p-values were subjected to multiple testing correction using the approach of Benjamini
and Hochberg (1995). All correlations with a final p-value below 0.05 and a coefficient rho ≥ 0.75
(positive correlation) or rho ≤ -0.75 (negative correlation) were counted as significant correlations.

22
Step 5: Significant correlations between subcommunities (SC vs. SC) as well as between
subcommunities and abiotic parameters (SC vs. Abio) were counted (Table 1 and Table 2) and
visualized for phases described in step 2 (Fig. S8.1).

Reference
Benjamini, Y., and Hochberg, Y. (1995) Controlling the false discovery rate: a practical and powerful
approach to multiple testing. J R Stat Soc Ser B 57: 289-300.
Buttigieg, P. L., and Ramette, A. (2014) A guide to statistical analysis in microbial ecology: a
community-focused, living review of multivariate data analyses. FEMS Microbiol Ecol 90: 543-550.
Clarke, K. R. (1993) Non-parametric multivariate analyses of changes in community structure. Austral
Ecol 18: 117-143.
Epskamp, S., Costantini, G., Haslbeck, J., Cramer, A. O. J., Waldorp, L. J., Schmittmann, V. D., and
Borsboom, D. (2017) qgraph: Graph Plotting Methods, Psychometric Data Visualization and
Graphical Model Estimation. R Package Version 1.4.3, [WWW document]. URL https://cran.r-
project.org/web/packages/qgraph
Günther, S., Faust, K., Schumann, J., Harms, H., Raes, J., and Müller, S. (2016) Species-sorting and
mass-transfer paradigms control managed natural metacommunities. Environ Microbiol 18: 4862-
4877.
Harrell, F. E. (2017) Hmisc: Harrell Miscellaneous. R Package Version 4.0-3, [WWW document]. URL
https://cran.r-project.org/web/packages/Hmisc
Koch, C., Günther, S., Desta, A. F., Hübschmann, T., and Müller, S. (2013) Cytometric fingerprinting
for analyzing microbial intracommunity structure variation and identifying subcommunity function.
Nat Protoc 8: 190-202.
Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., McGlin, D., et al. (2017) vegan:
Community Ecology Package. R Package Version 2.4-3, [WWW document]. URL https://cran.r-
project.org/web/packages/vegan
Revelle, W. R. (2017) psych: Procedures for Psychological, Psychometric, and Personality Research. R
Package Version 1.7.5, [WWW document]. URL https://cran.r-project.org/web/packages/psych

23
 A
1st phase 2nd phase (subsampled by drift events) 3rd phase
Drift 1 Drift 2 Drift 3
C1

C2

B
1st phase 2nd phase (subsampled by temperature changes) 3rd phase
T1 T2 T3 T4
40 °C
30 °C
7 18 28 43 49 60 70 81
D1

D2

D3

Figure S8.1 Significant correlations visualized as networks (R package ‘qgraph’) per defined phase. The first phase includes the adaptation of the community to reactor conditions. The last phase represents the communities in the reference spaces. The second phase subsamples according to A: drift events (Drift 1 – Drift 3 in C1 and C2)
or B: temperature changes (T1 – T4 in D1, D2 and D3). Significant correlations between dominant subcommunities (blue circle) and abiotic parameters (red circle) were represented as colored edges, were positive correlations are shown in green (rho ≥ 0.75 ) and negative correlations in red (rho ≤ -0.75).

24
S9: Comparison of different methods in calculating diversity metrics with cytometric data
1. Cytometric α- diversity
In a recent study, Props et al. (2016) showed that microbial community diversity calculation based on
single cell data can be determined by using order-based Hill numbers (Hill, 1973) and provide
ecological information comparable to 16S rRNA amplicon sequencing. In contrast to the 128 X 128
binning grid Props used, Liu et al. (2018) invented calculation of Hill numbers based on gate-based
data. Gate-based Hill numbers (Dq=0,1,2) per sample were calculated with Equation S9.1 to Equation
S9.3. The α-diversity was evaluated by using the numbers of all subcommunities of a community that
contain cells (which were practically all gates for q=0), and with the order q increasing (0 to 2), more
emphasize was placed on subcommunities with higher cell abundances while subcommunities with
low cell numbers were relatively ignored.

Equation S9.1: Dq=0 = 𝐺

𝐺
Equation S9.2: Dq=1 = 𝑒 − ∑𝑖=1 𝑝𝑖ln(𝑝𝑖)
1
Equation S9.3: Dq=2 = ∑𝐺 2
𝑖=1(𝑝𝑖 )

𝐺: Number of subcommunities per sample.

𝑝𝑖 : Relative cells abundance of the ith subcommunity.

In addition, α-diversity values can also be determined by using the cell numbers of only the dominant
subcommunities (cell abundance per gate above the average 1.47%). Testing the data of the five
reactors a comparable outcome between both approaches was found (Fig. S9.1). The diversity values
of high-order gate-based Hill numbers (Dq=1,2) for all subcommunities were the same to those
obtained of Dq=0 for dominant subcommunities, which proved the estimation of α-diversity values
using gate-based information and its suitability to indicate community structure changes over time.
An overview of all diversity metrics is shown in Table S9.1 (α-diversity: Hill numbers Dq=0,1,2 of all
subcommunities, and Dq=0 of dominant subcommunities). The α-diversity values were calculated with
an R-script (URL https://github.com/LiuZishu/MCFlowCytoAnalysis).

25
 70

C1
35

0
70

35 C2

0
70
Diversity value

35 D1

0
70

35 D2

0
70

35 D3

0
0 10 20 30 40 50 60 70 80 90
Time (d)

Figure S9.1: Variation of microbial community α-diversity over time. α-diversity values were calculated as gate-
based Hill numbers Dq=0 (square), Dq=1 (triangle) and Dq=2 (diamond) for all subcommunities (open symbols) and
Dq=0 for the dominant subcommunities (solid symbols) per reactor; C1: dark grey, C2: light grey, D1: blue, D2:
red, and D3: green.

26
Table S9.1: Overview of cytometric α-diversity values for all subcommunities (Dq=0,1,2) and dominant
subcommunities (Dq=0) for reactors C1, C2, D1, D2, and D3. For D1-D3 the temperature changes were shown
(35 °C: light red, 40 °C: dark red).
α - diversity
Time(d) Dominant subcommunities Dq=0 of all subcommunties Dq=1 of all subcommunties Dq=2 of all subcommunties
C1 C2 D1 D2 D3 C1 C2 D1 D2 D3 C1 C2 D1 D2 D3 C1 C2 D1 D2 D3
0 7 68 11.0 5.8
0.25 6 7 8 7 8 67 68 67 68 67 5.5 7.0 6.5 6.1 6.5 3.4 3.9 3.7 3.6 3.8
1 18 18 17 15 19 68 68 68 68 68 22.4 23.2 26.4 22.4 26.4 12.4 12.8 14.9 12.0 15.5
2 21 21 21 17 20 68 68 68 68 68 29.2 30.6 28.8 28.1 32.1 17.1 20.8 14.9 16.1 21.4
3 18 21 17 19 20 68 68 68 68 68 30.3 31.5 28.8 29.7 33.2 18.8 21.3 16.8 19.8 21.3
5 17 14 16 15 10 68 68 68 68 68 27.3 29.5 24.9 17.7 13.5 14.9 17.9 13.8 9.6 7.7
7 14 12 19 10 15 68 68 68 68 68 26.5 19.4 26.7 13.9 22.7 13.5 11.4 15.9 7.9 11.6
8 17 19 12 9 17 68 68 68 68 68 25.9 34.0 20.3 20.3 17.7 14.2 21.5 8.3 8.5 7.6
9 18 15 15 12 11 68 68 68 68 68 24.9 20.3 21.5 13.8 14.6 15.9 9.4 11.1 5.6 6.1
10 16 12 16 12 18 68 68 68 68 68 20.3 17.9 26.3 15.8 30.2 11.9 8.5 15.2 8.1 17.8
11 13 12 17 16 17 68 68 68 68 68 17.9 17.6 27.6 20.7 24.3 9.5 8.1 17.6 13.2 12.0
12 16 12 8 16 18 68 68 68 68 68 23.2 18.9 11.1 21.9 24.3 11.8 9.1 5.1 14.6 17.1
14 27 14 18 18 19 68 68 68 68 65 39.7 15.6 26.2 27.1 24.0 29.2 8.3 13.3 18.8 17.2
15 20 14 16 17 17 68 68 68 68 65 36.3 14.1 21.4 27.1 25.1 26.1 7.4 9.8 19.4 17.8
17 16 15 21 19 19 68 68 67 68 68 27.0 12.8 28.5 28.4 27.9 16.4 6.2 15.7 20.4 19.3
18 20 16 17 20 20 68 68 68 68 66 31.4 15.0 21.9 27.8 29.6 19.4 7.2 12.1 19.3 21.9
19 15 15 14 21 20 68 68 68 68 67 25.4 14.4 22.6 29.4 30.9 13.4 6.3 14.2 20.2 22.2
21 15 15 15 21 22 68 68 68 68 67 19.4 19.3 21.0 32.0 33.1 9.1 11.7 11.9 22.2 24.6
22 12 16 14 23 21 67 68 68 68 68 16.6 18.0 17.5 32.2 32.2 7.6 11.8 9.6 22.9 22.4
23 12 18 13 23 22 68 67 67 68 67 15.7 20.1 19.4 29.8 33.2 7.1 13.3 12.2 18.9 24.3
24 10 15 16 20 23 68 67 68 68 67 13.3 19.7 21.0 27.2 33.8 5.6 12.3 12.2 15.4 25.0
25 9 18 16 22 24 68 67 68 68 67 12.9 22.0 19.8 31.0 33.3 5.3 13.8 10.9 18.9 24.3
28 11 18 19 19 20 68 68 66 68 66 11.6 26.7 19.5 25.2 25.3 5.1 18.9 11.5 16.6 14.4
29 11 18 18 18 16 68 68 68 67 68 13.1 24.6 22.2 25.9 23.4 5.9 18.1 11.3 16.8 14.1
30 11 18 15 15 20 68 67 68 68 68 15.5 23.5 17.1 22.1 28.5 7.2 16.9 8.9 13.2 19.6
31 11 18 15 13 17 68 68 68 68 68 14.6 24.7 19.1 14.3 22.7 7.0 18.2 10.6 7.9 13.3
35 14 16 16 12 12 68 68 67 68 68 22.5 22.3 18.8 21.8 18.7 14.0 15.7 11.6 14.2 10.7
36 13 19 13 17 12 68 68 67 68 68 22.0 25.6 18.3 22.4 16.6 13.4 17.8 11.5 16.0 9.5
37 13 17 15 18 12 68 68 67 67 68 21.7 23.2 19.5 25.6 14.4 11.8 13.5 12.5 18.9 8.4
38 13 14 15 16 5 68 68 67 68 65 20.4 18.8 19.7 24.5 5.9 10.7 9.0 12.9 15.7 4.3
39 17 12 17 16 7 68 68 68 68 65 21.4 17.0 20.3 17.0 5.7 10.9 8.4 12.4 11.1 3.8
43 11 12 18 15 6 68 68 66 68 68 18.9 18.6 19.3 22.0 8.1 9.1 10.1 12.2 14.4 4.8
44 13 12 17 16 11 68 68 68 67 68 18.6 18.4 19.4 19.8 9.9 9.2 10.1 12.2 12.5 5.1
45 13 12 15 15 10 68 68 67 67 68 21.5 20.6 19.9 16.3 9.3 11.6 10.6 11.8 9.8 4.6
46 21 14 17 20 12 68 68 68 68 68 33.0 17.7 24.5 20.5 11.0 20.7 9.1 16.5 13.5 5.3
49 15 13 21 15 7 68 68 68 68 68 21.7 18.8 30.1 23.0 7.5 9.0 10.7 19.6 15.9 5.3
50 7 18 21 15 7 68 68 68 68 67 13.9 24.3 30.1 21.5 6.7 6.5 16.5 19.6 15.0 4.2
51 7 20 22 15 7 68 68 68 66 68 11.0 25.8 29.3 15.9 7.0 5.2 17.0 19.0 10.5 4.2
52 9 18 19 14 13 68 68 68 67 68 11.5 23.4 24.8 12.8 13.3 5.9 12.6 13.3 8.1 8.1
53 10 12 17 16 15 68 68 68 68 68 13.1 15.1 23.6 18.0 18.9 6.5 8.9 13.0 10.9 13.0
56 12 11 17 13 19 68 68 66 68 67 21.3 12.8 20.3 22.4 23.7 10.6 8.0 10.8 14.3 15.0
57 11 10 16 18 19 68 68 67 68 66 20.6 11.3 19.2 27.2 24.1 11.3 6.7 10.3 19.9 15.3
58 12 11 16 18 20 68 68 68 68 67 18.4 13.1 21.1 23.8 24.3 8.2 7.5 12.8 16.7 15.2
59 14 9 19 17 18 68 68 67 67 67 20.8 12.5 20.5 20.6 20.9 10.3 6.3 13.5 14.0 13.1
60 13 13 17 17 17 68 68 67 66 65 18.1 20.3 19.0 18.6 18.1 8.9 10.2 10.9 11.8 10.6
65 15 19 14 12 17 68 68 68 65 64 20.7 26.9 17.1 15.2 17.2 11.1 16.4 9.8 10.0 10.8
66 15 17 14 12 16 68 68 67 65 65 21.4 29.2 16.8 14.9 16.6 11.7 18.9 9.3 9.8 10.5
67 14 16 13 10 17 68 68 68 67 66 21.2 27.6 18.2 14.8 16.5 11.0 17.9 10.4 9.6 10.4
69 14 15 13 11 16 68 68 68 66 66 22.4 28.8 18.1 14.7 16.2 11.7 18.6 9.9 8.9 10.3
70 15 17 16 12 15 68 68 67 65 67 23.1 26.4 19.5 15.8 15.8 12.9 16.9 10.4 9.4 10.5
71 14 14 16 15 13 68 68 67 65 65 19.9 23.0 21.7 16.6 14.7 11.1 13.5 13.8 10.9 9.6
72 15 15 16 16 17 68 68 66 62 66 21.0 21.5 23.2 17.5 15.1 11.7 11.9 13.0 11.3 9.0
73 15 13 18 19 17 68 68 68 68 67 19.8 25.1 23.8 30.4 16.1 10.5 15.8 13.2 19.7 10.5
74 15 16 18 6 16 68 68 68 67 68 21.1 25.5 23.3 9.3 17.5 11.7 15.9 13.6 5.4 11.3
77 12 14 20 5 19 68 68 68 67 68 18.2 26.4 25.3 4.5 24.8 10.1 15.9 16.7 3.0 18.0
78 15 15 19 3 20 68 68 68 66 68 21.1 24.8 24.0 3.7 23.4 10.9 14.3 15.6 2.6 17.0
79 13 15 18 5 16 68 68 68 66 68 19.1 26.2 23.8 4.5 20.6 10.5 15.5 15.5 3.0 13.6
80 13 16 17 7 15 68 68 68 66 68 20.5 27.3 23.5 6.6 19.6 11.0 16.0 15.8 4.1 13.5
81 13 16 20 4 14 68 68 68 65 68 19.7 28.7 24.4 4.6 18.2 10.5 17.2 15.5 2.9 11.8
84 13 17 17 5 14 68 68 68 68 67 17.7 29.3 23.5 4.9 18.0 8.5 17.2 15.3 3.0 11.7
85 12 17 19 4 15 68 68 68 68 66 18.2 28.6 25.4 6.1 18.8 9.5 16.7 16.4 3.4 11.8
86 13 14 19 6 15 68 68 68 68 67 19.4 29.8 25.7 7.2 19.4 9.7 17.9 16.9 3.7 12.8
87 13 16 18 10 21 68 68 68 68 68 19.1 28.7 24.9 11.0 36.7 10.1 16.2 16.5 5.4 26.8
88 13 16 17 13 17 68 68 68 68 68 15.9 28.4 24.9 13.2 22.9 8.1 16.3 16.0 6.5 11.3
90 15 16 17 15 17 68 68 67 68 68 19.9 26.4 23.6 26.1 24.7 9.3 16.9 15.5 14.8 17.4
91 13 21 19 16 16 68 68 67 68 67 21.4 33.2 23.7 29.7 21.6 11.3 22.6 15.4 17.2 14.7
27
2. Cytometric β-diversity
The applied continuous cultivation conditions supported all cells that can survive a dilution rate of
0.72 d-1. However, their assembly was highly varying both between reactors (inter-community β-
diversity) and also over time within each reactor (intra-community β-diversity) which points to viable
neutral forces (Fig. 2B, 4B). To calculate their strength, inter-community β-diversity values, based on
pairwise comparison of samples of conjoint time points and which are from two identically operated
reactors (C1xC2, D1xD2, D1xD3, D2xD3), were compared to test for positive gradients in divergence,
and thus neutral behavior (Fig. 7). Values of intra- and inter-community β-diversity were given in
Table S9.2.

Table S9.2: Overview of cytometric β-diversity. Intra-community β-diversity: calculated by pairwise comparison
of samples along time within one reactor; Inter-community β-diversity: calculated by pairwise comparison of
samples at identical time points between reactors. For D1-D3 the temperature changes were shown (35 °C:
light red, 40 °C: dark red).
β - diversity
Time(d) Intra- community Inter- community
C1 C2 D1 D2 D3 C1×C2 D1×D2 D1×D3 D2×D3
0.25 1 1 0 1
1 12 11 9 8 11 0 4 2 6
2 11 11 10 12 9 0 4 7 5
3 11 8 12 6 10 7 6 5 7
5 13 15 11 24 20 5 23 20 7
7 15 10 11 5 17 16 21 16 11
8 9 9 11 7 6 20 13 19 16
9 1 18 9 9 6 9 21 16 13
10 2 7 3 10 9 10 20 14 24
11 3 4 15 6 17 5 13 14 9
12 5 2 9 6 5 6 14 16 12
14 17 2 12 8 5 19 16 21 15
15 9 0 6 1 2 12 13 17 12
17 10 3 11 8 8 19 12 6 10
18 8 1 4 7 5 18 17 11 12
19 7 1 7 7 4 16 15 10 11
21 4 6 5 8 4 14 20 13 15
22 3 3 3 8 1 12 17 11 10
23 2 4 1 2 3 12 20 11 11
24 2 5 3 5 9 11 16 11 13
25 3 3 6 2 3 13 14 12 12
28 4 2 7 7 8 13 10 13 15
29 2 4 5 9 6 13 10 10 14
30 0 0 5 5 4 13 6 15 15
31 2 2 0 6 13 17 6 12 10
35 5 6 5 9 15 10 12 22 16
36 1 5 5 15 2 12 12 21 19
37 2 6 4 3 6 10 9 17 14
38 4 5 0 4 7 15 9 18 13
39 6 4 2 6 2 13 15 18 9
43 6 4 3 9 3 9 13 22 13
44 4 0 3 7 5 13 17 22 9
45 4 6 4 5 1 11 16 19 9
46 14 6 2 11 4 27 15 19 12
49 12 23 8 5 5 18 18 26 8
50 10 9 2 6 2 17 16 22 12
51 0 8 3 8 2 21 17 21 8
52 2 8 3 3 6 17 19 18 9
53 3 10 6 10 8 18 15 14 7
56 2 5 8 13 8 19 12 12 8
57 3 3 3 5 2 17 10 9 7

28
Table S9.2 (continue): Overview of cytometric β-diversity.
β - diversity
Time(d) Intra- community Inter- community
C1 C2 D1 D2 D3 C1×C2 D1×D2 D1×D3 D2×D3
58 7 3 6 2 5 21 8 6 8
59 6 2 3 3 2 19 6 11 11
60 5 4 2 4 3 18 4 8 8
65 8 12 5 9 2 16 10 9 7
66 2 4 0 0 1 16 10 8 6
67 5 3 1 2 1 18 9 10 9
69 2 7 4 1 1 15 14 9 7
70 1 6 3 1 1 20 10 7 7
71 5 5 6 7 4 14 7 7 4
72 3 3 8 1 4 18 12 11 5
73 4 2 8 21 2 16 13 9 18
74 2 3 4 15 5 17 20 10 20
77 3 6 6 3 5 18 23 15 24
78 3 3 1 2 1 18 22 15 23
79 2 2 3 2 6 20 19 16 21
80 2 1 3 2 1 19 18 16 22
81 2 0 7 3 1 21 24 10 18
84 6 7 5 1 2 18 22 9 19
85 3 2 2 1 1 19 23 10 19
86 1 3 4 2 0 17 23 6 19
87 2 2 1 6 12 17 24 15 17
88 2 2 3 5 22 15 26 18 22
90 8 6 4 10 14 21 26 10 22
91 6 9 2 3 1 20 27 9 24

Reference
Hill, M. O. (1973) Diversity and evenness: a unifying notation and its consequences. Ecology 54: 427-
432.
Liu, Z., Cichocki, N., Bonk, F., Günther, S., Schattenberg, F., Harms, H., et al. (2018) Ecological stability
properties of microbial communities assessed by flow cytometry. mSphere 3: e00564-17.
Props, R., Monsieurs, P., Mysara, M., Clement, L., and Boon, N. (2016) Measuring the biodiversity of
microbial communities by flow cytometry. Methods Ecol Evol 7: 1376-1385.

29
S10: Quantification of community stability properties after drifts or disturbances
Drift events, by nature, are contradictory to stability of microbial communities. Among others,
deviation between samples within one reactor is one parameter that indicates instabilities. Using a
workflow from Liu et al. (2018), the height of deviations after drifts (i.e. C1 and C2) or disturbances
(i.e. D1, D2, and D3) was calculated. In short, Canberra distance values were determined by using the
numbers of subcommunities and their respective cell abundancies for pairwise comparison of
successive samples taken from one reactor. The community structure prior to a drift/disturbance
event was set as reference structure and, the respective time point as reference point, to calculate a
possible following deviation in structure. Two drifts were observed in C1 with day 14 (drift 1) and day
46 (drift 2) and three in C2 with day 9 (drift 1), day 49 (drift 2), and day 65 (drift 3). In the reactors
D1-D3, the subsampling was performed according to the temperature changes which were set up at
days 7 (T1), 28 (T2), 49 (T3) and 70 (T4).

C1

C2

D1

D2

D3

0 10 20 30 40 50 60 70 80 90
Time (d)
Figure S10.1: Scheme of drift/disturbance events that occurred in every reactor (C1: dark grey, C2: light grey,
D1: blue, D2: red, D3: green). Adaptation phases (dash lines) were not considered. White vertical lines marked
drifts (C1: 14 d and 46 d; C2: 9 d, 49 d, and 65 d) or the starting of parallel disturbance events in D1, D2 and D3
(at 7 d, 28 d, 49 d and 70 d). Black lines represented samples selected as reference points (C1: 12d and 45d; C2:
8d, 46d, and 60d; D1 – D3: 7d, 28d, 49, 70d).

Both drifts and disturbances caused deviations from the reference points (Fig. S10.2 A and B). Values
of deviations (dc) were summarized as a mean with standard deviation (mean ± SD, Table S10.1), and
as peak values for each event (dc.peak, Table S10.1). Another parameter of the stability concept is the
ability of a community to stay unchanged in the presence of a disturbance, which is described by the
term resistance. The ability to return to original community states after disturbance is described as
recovery (engineering resilience).

30
A 1.0
Deviation after drfits

0.5

0.0

B
Deviation after disturbances

1.0

0.5

0.0

0 10 20 30 40 50 60 70 80 90
Time (d)
Figure S10.2: Structural deviations from the reference points (black circles) were followed after drift events (A)
or disturbances (B). The radius of references space was determined by using the data of C1 (Fig. 1A) and
marked as dashed line (rc = 0.23). Points represent community states which deviated from the reference points
as the responding to drift events or disturbances. A: Community deviations after drift events in C1 (dark grey)
and C2 (light grey). B: Community deviations after disturbances in reactors D1 (blue), D2 (red) and D3 (green).

Table S10.1: Structural deviations, resistance (RS) and recovery (RC) were calculated within the successive time
period for each event as the workflow of Liu et al. (2018). Events were drifts in reactor C1 and C2 and
temperature changes (T1 - T4) in reactor D1, D2 and D3. Structural deviations from the reference points were
quantified as Canberra distances (dc); dc values were summarized (mean ± SD) and named the max value as
dc.peak per event.
Reference Time period
Reactor Event dc (mean ± SD) dc.peak RS RC
time point (days)
Drift 1 Day 12 33 0.43±0.05 0.49 0.51 0.02
C1
Drift 2 Day 45 46 0.35±0.03 0.43 0.57 0.13
Drift 1 Day 8 38 0.56±0.05 0.63 0.37 0.05
C2 Drift 2 Day 46 14 0.57±0.06 0.63 0.37 0.02
Drift 3 Day 60 31 0.51±0.03 0.57 0.43 0.09
T1 Day 7 21 0.56±0.12 0.66 0.34
T2 Day 28 21 0.47±0.05 0.55 0.45
D1
T3 Day 49 21 0.44±0.10 0.51 0.49 0.08
T4 Day 70 21 0.38±0.06 0.45 0.55 0.07
T1 Day 7 21 0.53±0.10 0.63 0.37
T2 Day 28 21 0.48±0.08 0.61 0.39 0.12
D2
T3 Day 49 21 0.51±0.06 0.56 0.44 0.04
T4 Day 70 21 0.65±0.14 0.77 0.23 0.05
T1 Day 7 21 0.54±0.11 0.66 0.34 0.04
T2 Day 28 21 0.60±0.14 0.74 0.26
D3
T3 Day 49 21 0.65±0.14 0.75 0.25 0.01
T4 Day 70 21 0.53±0.10 0.66 0.34 0.15

Reference
Liu, Z., Cichocki, N., Bonk, F., Günther, S., Schattenberg, F., Harms, H., et al. (2018) Ecological stability
properties of microbial communities assessed by flow cytometry. mSphere 3: e00564-17.

31
S11: Fates of subcommunities
1. Fate of unique subcommunities per reactor
The relative cells abundance per unique subcommunity was followed overtime and per reactor (Fig.
S11.1). For many subcommunities huge variations in cell numbers were found during the whole
experimental set up (e.g. G44 in D2), while other subcommunities seem to have been washed out
(e.g. G36 and G39) or remained at low abundance levels (e.g. G26).

32
Figure S11.1: Relative cells abundance of unique subcommunities per reactor (from left to right C1, C2, D1, D2,
and D3). Subcommunities are displayed according to the order of the mean value of relative cells abundance
per subcommunity of all samples.

33
2. Fate of dominant subcommunities in the inoculum
The fate of the dominant subcommunities in the inoculum was followed overtime per reactor (Fig.
S11.2). Seven dominant subcommunities of the inoculum (abundance above 1.47%, G14, G36, G37,
G38, G39, G40, G41) were found in all five reactors and maintained their abundancies at the start
with identical quantities. During the adaptation phase (7 days), these subcommunities lost their
abundance to values below 1.47% (dashed line, Fig. S11.2). With exception of subcommunity G14,
the other 6 starting dominant subcommunities were almost not dominant any more in continuous
cultivation.

Figure S11.2: Abundance variations of dominant subcommunities in the inoculum over time. Seven dominant
subcommunities (abundance above 1.47%, dash line, G14, G36, G37, G38, G39, G40 and G41) are marked by
different colors. Due to the adaptation process the starting dominant subcommunities became part of the rare
fraction of cells.

34
S12: Sequencing protocols and details
1. Sample preparation for sequencing analysis
Illumina® 16S rRNA gene amplicon sequencing was used for analysis of whole community and sorted
subcommunity composition. For the whole community analysis, 1 mL cell sample was taken from the
reactor and centrifuged (21,000 g, 10 min, 4 °C), the supernatant was discarded and the pellet frozen
at -20 °C until DNA isolation. Sorted subcommunity samples were harvested and stored according to
the given protocol (S5, cell sorting). In addition, to ensure the quality of data sets a mock community,
MBARC26 (Singer et al., 2016), was added to the sequencing project to track the quality of the whole
sequencing process.

2. DNA Extraction and quality testing

The DNA extraction procedure was performed according to the Chelex protocol (Lambrecht et al.,
2017). 70 µL 10% (wt/vol) Chelex 100 solution (Biorad, Hercules California, USA) was used to extract
DNA from about 500,000 cells (stored at -20 °C). The quality of the extracted DNA was tested by
visualizing the product of a preceding polymerase chain reaction (PCR) via gel electrophoresis (1.5%
agarose). This PCR step was done with 35 cycles in a S1000 Thermal cycler (Biorad, Hercules
California, USA) by using the universal primers Forward 27F 5'-AGAGTTTGATCMTGGCTCAG-3' and
Reverse 1492R 5'-TACGGYTACCTTGTTACGACTT-3' (Lane, 1991; Liu et al., 2018).

3. Library preparation for Illumina® MiSeq sequencing technique

The V3-V4 region of the bacterial 16S rRNA gene region was amplified with the primers Pro341F 5’-
CCTACGGGNBGCASCAG-3’ (Takahashi et al., 2014) and Pro805R 5’-GACTACNVGGGTATCTAATCC-3’
(Herlemann et al., 2011). These primers as well as the barcoded primers used for the library were
synthesized by Eurofins (Eurofins Scientific, Luxembourg City, Luxembourg) as well as the barcoded
primers for performing the library.
The PCR was performed with 20 pmol Forward and Reverse primers, 4 nmol of dNTP mix (Promega,
Fitchburg, Wisconsin, USA), 4 µL 5x Phusion® GC solution, 40 nmol of MgCl2 (both provided in the
polymerase kit), 0.4 units of Phusion® High-Fidelity Polymerase (New England Biolabs, Ipswich,
Massachusetts, USA) and 2 µL DNA solution. Nuclease free water (Qiagen, Velno, Netherlands) was
added to adjust the final reaction volume to 20 µL. The following PCR conditions were used: 3 min of
initial denaturation at 95 °C, a denaturation step at 95 °C for 30 sec, annealing at 52 °C for 60 sec,
extension for 1 min at 72 °C and a final extension at 72 °C for 10 min before storage at 4°C. The
sorted samples were run for 18 PCR cycles and the non-sorted samples for 15 cycles. More reaction
cycles of sorted samples are set according to the lower DNA content of DNA extracted solutions. For
each batch, a negative control without any DNA was amplified up to 35 cycles and checked via gel

35
electrophoresis (1.5% agarose) to ensure that no contamination was present. In absence of
contamination, the procedure continued by the purification of the amplicons using the Agencourt®
AMPure® XP-Kit (Beckman Coulter, Brea, California, USA) following their recommended protocol. The
purified DNA fragments were resuspended in nuclease free water (Qiagen, Velno, Netherlands) up to
10 µL. The second step PCR took place within the same conditions as mentioned above using 2 µL of
the purified amplicon solution and the sample-corresponded barcoded primers for 8 cycles. Again,
negative controls were run in parallel without DNA up to 35 PCR cycles to test potential
contaminations. Samples free of contamination were purified as described before. These DNA
fragments were quantified using the Qubit® 3.0 (Life technologies, Carlsbad, Califorania, USA) and
the HS DNA kit (Life technologies, Carlsbad, California, USA), to finally be equimolarly pooled and be
sequenced with MiSeq (Illumina®, San Diego, California, USA) at the Helmholtz Center for Infection
Research by the Genome Analytics Group (GMAK, HZI, Braunschweig, Germany). To minimize the
technical bias, every PCR was done in triplicates, purified and quantified separately before to be
pooled as a unique sample after both PCR steps. The mock community was also processed in the
same way.

4. Sequencing data evaluation procedure

The Illumina dataset was first quality trimmed with PRINTSEQ (Schmieder and Edwards, 2011) from
the 3’ side at a minimum of Q=30 in a window of 20 bases. The remaining sequences were
demultiplexed and merged using Mothur version 1.39 (Schloss et al., 2009). The pre-clustering step
helped us to remove the singletons from the data set and the chimeras were removed using UCHIME
(Edgar et al., 2011). The OTU classification was done with the Mothur’s average neighbour clustering
algorithm with a 97% sequence similarity cut off on the SILVA database version 128 (Quast et al.,
2013). The analysis based on a data set of 4,297,663 forward-reverse overlapped sequences. All the
raw data are available under the BioProject accession number: PRJNA437592.
The data set comprised 56 samples which enclosed 44 whole community samples (Inoculum: 1, C1:
11, C2: 9, D1: 6, D2: 11 and D3: 6, Table S12.1) and 12 sorted subcommunities (Table S12.1). The
obtained data set comprised samples between 10,576 (Samp08, Table S12.1) and 326,254 cleaned
reads (Samp029, Table S12.1), therefore the number of 10,576 was kept as threshold for
normalization to allow diversity comparison between samples. Due to this, normalization was
performed and the rarefaction curves of all samples before and after normalization are shown in Fig.
S12.1 A and B. After normalization, sequences were classified on the class level where a total of 72
OTUs was determined as shown by the rarefaction curves in Fig. S12.1 C.
To ensure the quality of the sequencing run as well as the sequencing analysis on the whole data set,
the mock community MBARC26 (Singer et al., 2016) was part of the pooled samples. 93,800

36
sequences were obtained from the mock community control. Contamination sequences were found
at a maximum abundance of 0.35%. According to this result and following the guidelines of Bokulich
et al. (2013) we determined our OTU threshold at 0.35% meaning that the sequences below this
abundance threshold per class within a sample can not be taken into account. Applying this threshold
to the 56 sample set, 23 OTUs were assigned at the class level (OTU01 - 23, Table S12.2).

Table S12.1: List of whole community samples and sorted samples with their respective information. Reference
numbers of samples (Samp01 - 56), reactors (C1, C2, D1, D2 and D3), sampling time (day), number of cleaned
reads and identities: whole community (WC) or sorted subcommunity (grey shading).
Number of Number of
Ref NO. Reactor Time Identity cleaned Ref NO. Reactor Time Identity cleaned
reads reads
Samp01 Inoculum 0 WC 102,963 Samp29 D2 7 WC 326,254
Samp02 C1 1 WC 47,422 Samp30 D2 28 WC 77,392
Samp03 C1 7 WC 35,543 Samp31 D2 31 WC 102,468
Samp04 C1 14 WC 55,318 Samp32 D2 39 WC 52,845
Samp05 C1 28 WC 53,036 Samp33 D2 49 WC 108,427
Samp06 C1 31 WC 40,894 Samp34 D2 60 WC 107,230
Samp07 C1 39 WC 56,732 Samp35 D2 70 WC 47,082
Samp08 C1 49 WC 10,576 Samp36 D2 73 WC 93,334
Samp09 C1 60 WC 77,852 Samp37 D2 81 WC 76,247
Samp10 C1 70 WC 32,038 Samp38 D2 90 WC 88,880
Samp11 C1 81 WC 62,805 Samp39 D3 1 WC 49,029
Samp12 C1 90 WC 31,472 Samp40 D3 7 WC 24,802
Samp13 C2 1 WC 72,309 Samp41 D3 28 WC 49,222
Samp14 C2 7 WC 59,141 Samp42 D3 49 WC 72,734
Samp15 C2 14 WC 87,068 Samp43 D3 70 WC 68,513
Samp16 C2 28 WC 66,920 Samp44 D3 90 WC 66,225
Samp17 C2 31 WC 65,779 Samp45 C1 49 G5 52,523
Samp18 C2 49 WC 37,375 Samp46 C1 21 G8 42,368
Samp19 C2 70 WC 63,599 Samp47 C1 28 G8 45,947
Samp20 C2 81 WC 92,194 Samp48 C2 39 G4 35,468
Samp21 C2 90 WC 96,114 Samp49 C2 46 G4 56,337
Samp22 D1 1 WC 36,691 Samp50 C2 81 G4 92,241
Samp23 D1 7 WC 48,561 Samp51 C2 60 G8 17,227
Samp24 D1 28 WC 90,674 Samp52 C2 81 G9 61,482
Samp25 D1 49 WC 44,906 Samp53 C2 60 G45 251,528
Samp26 D1 70 WC 45,446 Samp54 C2 81 G45 75,164
Samp27 D1 90 WC 19,816 Samp55 D2 90 G30 93,547
Samp28 D2 1 WC 54,677 Samp56 D2 81 G44 45,115

37
 A B

C D

Figure S12.1: Rarefaction curves per sample without singletons. A: before normalization to 10,576 reads (green
line); B: after normalization; C: after classification to class level; D: after removing OTUs below the abundance
threshold of 0.35%.

Table S12.2: List of classified phylotypes (OTU 01-23) and their sequence abundances in the 56 samples;
unclassified sequences were shaded with grey color.
Ref NO. Domain Phylum Class Sequences
OTU 01 Bacteria Proteobacteria Alphaproteobacteria 255013
OTU 02 Bacteria Proteobacteria Betaproteobacteria 108218
OTU 03 Bacteria Proteobacteria Gammaproteobacteria 73163
OTU 04 Bacteria Bacteroidetes Sphingobacteriia 70161
OTU 05 Bacteria Bacteroidetes Flavobacteriia 22492
OTU 06 Bacteria Saccharibacteria 18022
OTU 07 Bacteria Actinobacteria Actinobacteria 9204
OTU 08 Bacteria Acidobacteria Blastocatellia 7763
OTU 09 Bacteria Bacteroidetes Cytophagia 6518
OTU 10 Bacteria Firmicutes Bacilli 4896
OTU 11 Bacteria Proteobacteria Deltaproteobacteria 3677
OTU 12 Bacteria Gracilibacteria 2037
OTU 13 Bacteria Chlorobi Chlorobia 1545
OTU 14 Bacteria Firmicutes Clostridia 552
OTU 15 Bacteria Verrucomicrobia Verrucomicrobiae 549
OTU 16 Bacteria Actinobacteria Acidimicrobiia 242
OTU 17 Bacteria Thermotogae Thermotogae 231
OTU 18 Archaea Euryarchaeota Halobacteria 209
OTU 19 Bacteria Chloroflexi Caldilineae 197
OTU 20 Bacteria Cyanobacteria ML635J-21 133
OTU 21 Bacteria Chloroflexi Anaerolineae 106
OTU 22 Bacteria Nitrospirae Nitrospira 54
OTU 23 Bacteria Chloroflexi Chloroflexia 39
OTU 24 Unclassified 1585
38
5. Comparison of MiSeq and flow cytometry data

a) Community structures revealed by phylotypes on the class level

Using class affiliation and their relative sequence abundance, community compositions of whole
community samples as well as sorted subcommunities were shown in Figure S12.2 A and B
respectively.

Figure S12.2: Taxonomical assignment based on the bacterial 16S rRNA genes (V3-V4 regions) and sequenced
with Illumina® Miseq. The number of sequences was normalized to 10,576 reads per sample, the OTU threshold
was set to 0.35%, and 23 OTUs were assigned to the class level (Tab. S12.2). A: Relative abundance of bacterial
communities from whole community samples per reactor (C1, C2, D1, D2 and D3) per day. For the reactors D1
to D3 the temperature disturbance frequencies are shown. B: Relative abundance of bacterial subcommunities
per reactor, per day, and per sorted gate. For each sample the rarefaction curve (black line) is shown as overlay.

The community inoculum in this study originated from the activated sludge of a nearby wastewater
treatment plant (see S1) and showed the typical phylogenetic composition (Hu et al., 2012) of
dominant Alpha-, Beta- and Gamma-proteobacteria, and Bacteroidetes (Sphingobacteria and
Flavobacteria; Fig. S12.2 A). In addition, other known phylotypes of wastewater communities such as
Saccharibacteria TM7 (Ferrari et al., 2014), Actinobacteria (Wagner et al., 2002), Blastocatellia (Gao
et al., 2016) and Cytophagia (Shu et al., 2015) were found with high abundance in our reactor
samples.

39
Consideration of abiotic factors can help us to draw hypothesis about distinct community functions.
For example, the presence of ammonium oxidizing bacteria (AOB) can be assumed because the
ammonium concentrations decreased in the control reactors and in D2 from about 30 mg N L -1 to
nearly zero (Fig. S3.1). AOB and nitrite oxidizing bacteria (NOB) can be expected to have high
abundances in a wastewater treatment plant. However, only the class Nitrospira was found in the
inoculum with an abundance of 0.51% (54 reads out of 10,576) and this phylotype was not found in
any of the other reactor samples with the given OTU threshold of 0.35%. Since AOB and NOB are
known to belong to the rare biosphere in many ecological situations (1 - 3%, Sanchez, 2016; Ye et al.,
2011) and to grow with small growth rate (e.g. μmax = 1.4 d-1 for AOB and 0.9 d-1 for NOB, Moussa et
al., 2005) we can expect, them to have a low abundance in our set-ups and to be washed out
(dilution rate = 0.72 d-1). Therefore, we lowered the threshold to zero to find related reads for these
bacteria in the normalized sample sets. For the class Nitrospira, two reads were found in D2 at day 31
(whole community sample) and one read in C2 at days 39 and 81 (subcommunity G4), respectively. In
addition, AOB from the genus Nitrosomonas were also found with 2 reads in the inoculum and 1 read
in C1 (at days 28 and 49). The presence of such reads can highlight the existence of NOB and AOB in
our reactors and their active ammonium oxidizing function.
Polyphosphate-accumulating organisms (PAOs) were reported to be closely related to Beta-
proteobacteria (genus Rhodocyclus, Crocetti et al., 2000; Candidatus Accumulibacter phosphatis,
Martín et al., 2006) and Actinobacteria (genus Propionibacter, Crocetti et al., 2000). In addition, some
reports (Mulkerrins et al., 2004; Li et al., 2010) have shown that in the biological phosphorus removal
process, PAOs were found to function preferably in the mesophilic or even psychrophilic range.
Temperature increases from 20 °C to 35.5 °C seem to rather decrease the phosphorus removal rates
(Panswad et al., 2003). Bacteria belonging to the class Beta-proteobacteria and Actinobacteria were
commonly present in all five reactors and we even found higher values of total phosphate in the
supernatant (PHO, mg P L-1, S3) of the disturbed reactors (D1:64.4 ± 2.3; D2:64.8 ± 2.3; D3:64.3 ± 2.3)
in comparison to those in control reactors (C1: 61.5 ± 2.6; C2: 62.4 ± 2.5) through the time.
Flow cytometry clearly showed community structure shifts for all five reactors due to the adaptation
process of the inoculum to the reactor conditions. Comparable shifts (Fig. S12.2 A) were also found in
the sequence data set with high abundances of Flavobacteria (27 - 33.1%) and Gamma-
proteobacteria (33.3 - 43.6%) at the first day of the experiment (Fig. S12.2 A). Following, a decrease
in abundance for the Flavobacteria to nearly zero in all reactors could be observed and also for the
Gamma-proteobacteria to 0.9 - 5.6% (C1 and C2) as well as to 0.5 - 32.3% (D1 to D3, 7 d - 90 d, Fig.
S12.2 A). Furthermore, and according to the class numbers of samples (Fig. S12.2 A), it can be
concluded that community diversity (richness) was finally maintained in the five reactors as was also
observed by the cytometric α-diversity. In addition, the speciation events in D3 at day 49 and D2 at

40
day 81 that caused an intermittent drop down of diversity, was observed both on the class (Alpha-
proteobacteria, Fig. S12.2 A) and the cytometric levels (G44 and G45, Fig. 2B and 4B).

b) Community assembly revealed by phylotypes on the class level

With the data on the class level, a dissimilarity analysis was performed for the whole community
samples using the same protocol applied for the cytometric data (Fig. 1B and 3B). In addition,
canonical correspondence analysis (CCA, Ter Braak and Verdonschot, 1995) was made with Miseq
data of whole communities and data of corresponding abiotic parameter measurements (T, OD, NH4,
PHO, CODt, CODs and CODb) to indicate the deterministic influence of the temperature stressors.
CCA performed in R program with the vegan package (cca{vegan}). Results of dissimilarity analysis
and CCA were shown as Figure S12.3 A and B, in respectively.

Figure 7 A: Dissimilarity analysis and B: Canonical correspondence analysis (CCA) on class level for 44 whole
community samples. Samples from each reactor were colored differently with C1: dark grey, C2: light grey, D1:
blue, D2: red, D3: green and inoculum: black. The size of points is increasing with sampling times. The
cultivation temperature was represented by the color density of the points: 30 °C (no color), 35 °C (transparent
color) and 40 °C (opaque color). Corresponding values for abiotic parameters were included in the CCA analysis.

A comparable pattern was found where similar trends in community assembly revealed the
consistence of the cytometric data (Fig. S12.3 A). Samples from the adaptation phase clustered apart
from the continuously grown communities, with the samples from the control reactors being clearly
apart from the disturbed ones. According to CCA results (Fig. S12.3 B), the deterministic influence of
the temperature disturbance was revealed. The increase in temperature was found to have
influences on other abiotic parameters such as causing increasing values for electrical conductivity,
pH, phosphorus, and ammonium which can, vice versa, be suspected to influence also the community
compositions. Similar to the cytometric data we found a clear unified effect of high temperature on
decreasing variations among disturbed reactors (Fig. 5).
41
Although Miseq data generated comparable results to flow cytometric data, it is still remarkable that
the numbers of samples that underwent the Miseq analysis were too low to show the dynamic
variation of the communities with the same resolution as was produced by the cytometric analyses.

Reference
Bokulich, N. A., Subramanian, S., Faith, J. J., Gevers, D., Gordon, J. I., Knight, R., et al. (2013) Quality-
filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat Methods 10:
57-59.
Crocetti, G. R., Hugenholtz, P., Bond, P. L., Schuler, A., Keller, J., Jenkins, D., and Blackall, L. L. (2000)
Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes
for their detection and quantitation. Appl Environ Microbiol 66: 1175-1182.
Edgar, R. C., Haas, B. J., Clemente, J. C., Quince, C., and Knight, R. (2011) UCHIME improves sensitivity
and speed of chimera detection. Bioinformatics 27: 2194-2200.
Ferrari, B., Winsley, T., Ji, M., and Neilan, B. (2014) Insights into the distribution and abundance of
the ubiquitous candidatus Saccharibacteria phylum following tag pyrosequencing. Sci Rep, 4: 3957.
Gao, P., Xu, W., Sontag, P., Li, X., Xue, G., Liu, T., and Sun, W. (2016) Correlating microbial community
compositions with environmental factors in activated sludge from four full-scale municipal
wastewater treatment plants in Shanghai, China. Appl Microbiol Biotechnol 100: 4663-4673.
Herlemann, D. P., Labrenz, M., Jürgens, K., Bertilsson, S., Waniek, J. J., and Andersson, A. F. (2011)
Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME J 5:
1571-1579.
Hu, M., Wang, X., Wen, X., and Xia, Y. (2012) Microbial community structures in different wastewater
treatment plants as revealed by 454-pyrosequencing analysis. Bioresour Technol 117:72-79.
Lambrecht, J., Cichocki, N., Hübschmann, T., Koch, C., Harms, H., and Müller, S. (2017) Flow
cytometric quantification, sorting and sequencing of methanogenic archaea based on F420
autofluorescence. Microb Cell Fact 16: 180.
Lane, D. J. (1991) 16S/23S rRNA sequencing. In Nucleic Acids Techniques in Bacterial Systematics.
Stackebrandt, E., and Goodfellow, M. (eds). Chichester, UK: John Wiley & Sons Ltd.
Li, N., Ren, N. Q., Wang, X. H., and Kang, H. (2010) Effect of temperature on intracellular phosphorus
absorption and extra-cellular phosphorus removal in EBPR process. Bioresour Technol 101: 6265-
6268.
Liu, Z., Cichocki, N., Bonk, F., Günther, S., Schattenberg, F., Harms, H., et al. (2018) Ecological stability
properties of microbial communities assessed by flow cytometry. mSphere 3: e00564-17.
Moussa, M. S., Hooijmans, C. M., Lubberding, H. J., Gijzen, H. J., and Van Loosdrecht, M. C. M. (2005)
Modelling nitrification, heterotrophic growth and predation in activated sludge. Water Res 39:
5080-5098.
Mulkerrins, D., Dobson, A. D. W., and Colleran, E. (2004) Parameters affecting biological phosphate
removal from wastewaters. Environ Int 30: 249-259.
Panswad, T., Doungchai, A., and Anotai, J. (2003) Temperature effect on microbial community of
enhanced biological phosphorus removal system. Water Res 37: 409-415.
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., et al. (2012) The SILVA ribosomal
RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:
D590-D596.
Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., et al. (2009)
Introducing mothur: open-source, platform-independent, community-supported software for
describing and comparing microbial communities. Appl Environ Microbiol 75: 7537-7541.
Schmieder, R., and Edwards, R. (2011) Quality control and preprocessing of metagenomic datasets.
Bioinformatics 27: 863-864.

42
Singer, E., Andreopoulos, B., Bowers, R. M., Lee, J., Deshpande, S., Chiniquy, J., et al. (2016) Next
generation sequencing data of a defined microbial mock community. Sci Data 3: 160081.
Sanchez, O. (2016) Bioremediation of Wastewater: Factors and Treatment. Oakville, Canada: Apple
Academic Press.
Shu, D., He, Y., Yue, H., and Wang, Q. (2015) Microbial structures and community functions of
anaerobic sludge in six full-scale wastewater treatment plants as revealed by 454 high-throughput
pyrosequencing. Bioresour Technol 186: 163-172.
Takahashi, S., Tomita, J., Nishioka, K., Hisada, T. and Nishijima, M. (2014) Development of a
prokaryotic universal primer for simultaneous analysis of bacteria and archaea using next-
generation sequencing. PLoS One 9: e105592.
Ter Braak, C.J., and Verdonschot, P.F. (1995) Canonical correspondence analysis and related
multivariate methods in aquatic ecology. Aquat Sci 57: 255-289.
Wagner, M., Loy, A., Nogueira, R., Purkhold, U., Lee, N., and Daims, H. (2002) Microbial community
composition and function in wastewater treatment plants. Antonie Van Leeuwenhoek, 81: 665-680.
Ye, L., Shao, M. F., Zhang, T., Tong, A. H. Y., and Lok, S. (2011) Analysis of the bacterial community in a
laboratory-scale nitrification reactor and a wastewater treatment plant by 454-
pyrosequencing. Water Res 45: 4390-4398.

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