MICROBIOLOGY RECALLS

1. Characteristics of fungi – AOTA

2. Septate hyphae- divisions
3. Molds w/ aseptate hyphae produce a specialized hyphal structure –sporangiophore
4. Basic, branching, interwinning structures of molds – mycelium
5. Mycelia w/in the colony that grow into the substrate – vegetative hyhae
6. Smallest RNA – enterovirus
7. RNA consists – DNA, RNA
8. High CO2 – capnophiles (5-10%)
9. Facultative anaerobe – grow either in presence /absence of CO2
10. Microaerophile – require little O2 to survive
11. Reduced, preformed, organic molecules from other bacteria- heterotrophs
12. Utilize energy produced by organic or inorganic compounds –chemotrophs
13. Common temp. for pathogenic bacteria – mesophile
14. Heat loving bacteria – thermophile
15. 0-2 degree celcius –psychrophile
16. Alkaline peptone –complex media
17. Ideal incubation – 35
18. Start biosynthesis – Lag phase
19. Growing/dividing – log phase
20. Nutrient – stationary phase
21. Simplest method sterilization – autoclave
22. Indicator autoclave – B. stearothermophilus
23. Organisms normally do not cause disease in their natural habitat to a healthy person –
opportunistic pathogen
24. Bacteria – prokaryotic
25. S. aureus – B. lactamate
26. Kirby-bauer test – filter paper disks
27. S.auerus- TSS syndrome
28. Exfoliative S.aurues- scalded skin syndrome
29. UTI in young; Resistant novobiocin – S.saprophyticus
30. Latex agglutination- clumping and protein A
31. S.aureus appearance in SBA – Bhemolytic
32. Lancefield is based on – antigen cell wall
33. Animal virulence factor – Hyalurodinase
34. Methyl Red test indicator- methyl red
35. Coffee/kidney/bean shaped – Neisseria
36. Virulence factor N.gonorrhea – common pili
37. Main cause of meningitis – H.influenza
38. P.aeruginosa incubation- 42
39. Found in “raw milk”- Brucella
40. Leptospirosis reference method – MAT
41. B.pertusis – whooping cough
42. Clue cells – G.vaginalis
43. Acid fast organism best method – kinyoun
44. Preferred diff S.aureus from S.epidermidis – coagulase test
45. Quellung test – capsular swelling
46. Specimens for N.gonorrhea – AOTA (eyes, rectum, oral cavity)
47. Chinese letters “palisade” –corynebacterium
48. Babes-ernst - C.diptheria
49. Acid fast bacilli retain dye- carbol fuschin
50. “rice water”- vibrio cholera
51. Aseptic technique – Pasteur
52. Septic technique – Lister
53. Bacillus antracis discovery – R.koch
54. pH bacteria -6.5-7.5
55. pH fungi – 5-6
56. Motility test – wet mount and hanging drop
57. Ethylene oxide – gas
58. VF of S.pneumoniae – capsule
59. Traveler’s diarrhea – E.coli
60. Salmonella antigens – S, O, Vi antigen
61. T. pallidum motility – corkscrew motility
62. Causes chanroid w/c STD charac. By paniful and tender genital lesions that advance to
ulcers with satellite lesions – H.ducreyi
63. W/ V growth factor – H.parahemolyticus
64. Gum/dental infection – periodonditis
65. Appear B.anthracis – Medusa head
66. European relapsing fever – B.recurrentis
67. Lyme disease – B. burgdorferi
68. Severe form of illness that affects the liver and kidney – Icteric leptospirosis/weil
syndrome
69. Havernhill fever- Streptobacillus molliformnis
70. Light microscope –
71. Serve as energy source – inclusion bodies
72. Ocular brightfield microscope – 10x
73. Measure relative velocity at w/c light passes through a material – refractive index
74. Compensate for the diff. in observer’s sight/vision bet.the left and right eyes
75. Controls the amount of light entering the condenser – consider iris diaphragm dial
76. Utilized in observation of chlamydiae, legionellae, mycobacteria, fungi – fluorescent
microscope
77. Detect T.pallidum – dark-field microscopy
78. 100,000x magnification – electron microscopy
79. Mistaken as lymphocyte – Cryptococcus
80. Bacteria unit – um
81. S.aereus lyses – RBC, Plts, macrophages, ;AOTA
82. Cause PINTA – T.carateum
83. Confirmatory test – treponemal test
84. B.pseudomallei – whitmore’s disease
85. Miscarriage – L.monocytogenes
86. Aerotolerant – P.acnes
87. Neutralize the antimicrobial – thiol broth
88. Viruses contain – DNA /RNA
89. Viruses according to shape – helical/polyhedral
90. Negri bodies – rabies/rhabdoviridae
91. Dark granules – metachromatic/ black
92. Flagella all over the organisms – peritrichous
93. Has calcium dipicolinate – clostridia
94. Stain for acid-fast bacilli – methylene blue
95. Positive for acid fast bacilli – red/pink
96. Most pathogenic bacteria – mesophile
97. Bacteria classification that uses energy from organic molecules – heterotrophs
98. SPS –prevents phagocytosis and complement activity
99. Incubator temp for CSF-
100. Deoxychocolate agar for –enterobacteriaceae
101. CTBA is for –
102. Antibiotic class for treatment of M. tuberculosis -
103. Coagulase test plasma –
104. Distinguish micrococcus from strep –
105. Grows on 6.5% NaCl – enterococcus
106. Leptospira test – culture
107. Whooping cough – B. pertussis
108. Interwinning – mycelium
109. Staphylococcus differential from streptococcus – catalase negative
110. The same coagulase with S.aureus – S.intermedius
111. Difference bet. S.aureus and S. intermedius
112. Similar morphologic physiologic and genetic traits into specific group –
classification
113. Bacterial genetics – DNA and RNA
114. Stains capsule –negative staining
115. Most pathogenic : facultative anaerobe
116. Fungi exhibit sexual phase – perfect fungi