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Microbial Nutrition and Growth: Nutrition Obtaining Required Substances From The Environment
Microbial Nutrition and Growth: Nutrition Obtaining Required Substances From The Environment
Growth
Assist. Prof. Dr. Abbas Obaid Al-Janabi
College of Anbar Medicine
IRAQ-2020
• Phosphorous – Mineral
deposits
• Sulfur – Minerals, H2S
• Metal Ions - Minerals
Mineral Nutrients Important in
Microbial Metabolism
• Potassium – essential to protein synthesis
and membrane function
• Sodium – used in some types of cell
transport
• Calcium – cell wall and endospore stabilizer
• Magnesium – component of chlorophyll;
membrane and ribosome stabilizer
• Iron – component of proteins of cellular
respiration
• Zinc, copper, nickel, manganese, etc.
Growth Factors
• Organic compounds that cannot be
synthesized by an organism & must be
provided as a nutrient
– essential amino acids, vitamins
Nutritional Types
Growth of a population at an
exponential rate under optimal
conditions
Stages in Normal Growth
• Lag phase
• Exponential/log phase
• Stationary phase
• Death or decline
Mathematics of Population Growth
Growth Curve
Methods for Monitoring Growth
• Dilution Plating
• Turbidity analysis using
spectrophotometer
• Direct count with hemacytometer
• Optical detection – Coulter Counter
Turbidity
Direct Microscopic Count
Electronic Counting
It is Ideal to Perform Most
Manipulations of a Culture When
it is Growing in Log Phase