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11. Statement I: PCR stands for polymerase 15. Statement I: In gene gun method cells
chain reaction are bombarded with high velocity
Statement II: In PCR multiple copies of microparticles of gold or tungsten coated
gene of interest is synthesized in vitro with DNA
two set of primers Statement II: Gene gun is also known as
[NCERT Page No-172] biolistic method
(a) Both the statements are correct [NCERT Page No-171]
(b) Statement I is correct but statement (a) Both the statements are correct
II is incorrect (b) Statement I is correct but statement
(c) Statement II is correct but statement II is incorrect
I is incorrect (c) Statement II is correct but statement
(d) Both the statements are incorrect I is incorrect
(d) Both the statements are incorrect
12. Statement I: In PCR a thermostable
DNA polymerase which remains active at 16. Statement I: In a method known as
lower temperature is employed microinjection recombinant DNA is
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Neutron Neet 2024 Zoology Sankalp
Biotechnology: Principle and processes
PNS – 02
directly injected into nucleus of animal I is incorrect
cell (d) Both the statements are incorrect
Statement II: Isolation of genetic
material from bacterial cell can be 20. Statement I: In a cloning vector it needs
achieved by treating them with lysozyme to have preferably one or more than one
[NCERT Page No- 171] restriction site for commonly used
(a) Both the statements are correct restriction enzymes
(b) Statement I is correct but statement Statement II: DNA is a hydrophobic
II is incorrect molecule it can’t pass through cell
(c) Statement II is correct but statement membranes directly
I is incorrect [NCERT Page No-169&170]
(d) Both the statements are incorrect (a) Both the statements are correct
(b) Statement I is correct but statement
17. Statement I: The Ti plasmid of II is incorrect
agrobacterium has now been modified (c) Statement II is correct but statement
into a cloning vector I is incorrect
StatementII: Agrobacterium (d) Both the statements are incorrect
tumefaciens a pathogen of monocots
plants causes tumor in these plants 21. Statement I: A stirred tank reactor is
[NCERT Page No-170] usually cylindrical or with a curved base
(a) Both the statements are correct Statement II: The most commonly used
(b) Statement I is correct but statement bioreactors are of stirring type
II is incorrect [NCERT Page No-174]
(c) Statement II is correct but statement (a) Both the statements are correct
I is incorrect (b) Statement I is correct but statement
(d) Both the statements are incorrect II is incorrect
(c) Statement II is correct but statement
18. Statement I: If any protein encoding I is incorrect
gene is expressed in heterologous host it (d) Both the statements are incorrect
is called recombinant protein
Statement II: The cells can be multiplied 22. Statement I: Transformation is a
in a continuous culture system to obtain procedure through which a piece of DNA
the desired product is introduced in host bacterium
[NCERT Page No-173] Statement II: pBR322 has restriction
(a) Both the statements are correct sites for both Hind1 and HindII
(b) Statement I is correct but statement [NCERT Page No-169]
II is incorrect (a) Both the statements are correct
(c) Statement II is correct but statement (b) Statement I is correct but statement
I is incorrect II is incorrect
(d) Both the statements are incorrect (c) Statement II is correct but statement
I is incorrect
19. Statement I: The most commonly used (d) Both the statements are incorrect
matrix in gel electrophoresis is agarose
Statement II: DNA separated by gel
electrophoresis can be visualized only 23. Statement I: Selection of transformants
after staining from non transformants can be done by
[NCERT Page No-168] antibiotic resistance gene
(a) Both the statements are correct Statement II: In insertional inactivation,
(b) Statement I is correct but statement protein gets inactivated
II is incorrect [NCERT Page No-170]
(c) Statement II is correct but statement (a) Both the statements are correct
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Biotechnology: Principle and processes
PNS – 02
(b) Statement I is correct but statement
II is incorrect
(c) Statement II is correct but statement
I is incorrect
(d) Both the statements are incorrect
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Neutron Neet 2024 Zoology Sankalp
Biotechnology: Principle and processes
PNS – 02
Answer Key
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