GENERAL BIOLOGY 1

Laboratory Worksheet 1: The Compound Light Microscope

2nd Period, A.Y. 2023-2024

Name: _____________________________________ Section: _______________________________

Professor: ___________________________________ Date Performed:
_______________________ Score:
A. Label the parts of the microscope. (18 points)
/65

White-
Blue-
Red-
Yellow-

https://www.walmart.com/ip/AmScope-40X-1600X-LED-Binocular-Compound-Microscope-
New/39549420
Figure 1. The parts of a compound light microscope

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Laboratory Worksheet 1: The Compound Microscope
Senior High School Department
2nd Period/ A.Y. 2023-2024
B. Calibration of the Microscope (Ocular Micrometer Calibration) (15 pts)

Objective Number of stage Number of Calibration of 1 Ocular Division

micrometer ocular divisions
divisions super superimposed =#Stage Micrometer Divisions X 10 um
imposed over over with stage #Ocular Micrometer Divisions
with ocular micrometer.
micrometer (4 pts) (4 pts)
(4pts)
Scanner

LPO

HPO

OIO

A. Onion cell (Allium cepa) under HPO (3pts)

Size of 1 cell: _________um
Calculation:

C. Using the microscope: 3 points each for the drawing (3x3=9)

Criteria 3 points 2 points 1 point
Color
The color of the drawing reflects
that of the image seen.
Completeness
The student satisfies all The student satisfies 2 The student satisfies only 1
The drawing shows the entire image
the three criteria listed of the criteria listed of the criteria listed
as seen under the microscope.
Accuracy
The drawing is detailed with all parts
presented.

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Laboratory Worksheet 1: The Compound Microscope
Senior High School Department
2nd Period/ A.Y. 2023-2024
 Letter “e” before viewing

Sketch of the letter e using the LPO. Sketch of the letter e using the HPO

GUIDE QUESTIONS: Be concise in answering the following questions.

Rubric for the 1st three questions and Q5:

2 points- if the response is correct
0 point- if the response is incorrect

1. What happens to the position of letter e when viewed under the microscope? Is it the same as
when viewed with the naked eye or is it inverted? (2 points)

2. When you move the slide to the left, in which direction does the letter image appear to move?
(2 points)

3. When you move the slide towards you, in which direction does the letter image appear to
move? (2 points)

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Laboratory Worksheet 1: The Compound Microscope
Senior High School Department
2nd Period/ A.Y. 2023-2024
4. Explain your observations on the three questions above. (3 points)
Rubric
3 points-Explanation is clear with correct supporting details.
2 points- Explanation is somewhat clear with few correct supporting details.
1 point- Explanation is vague with no supporting details.

5. When you shift to HPO, what happens to the letter image? How about the field of view? (2
points)

The letter image _____________________

Field of view_________________________

6. What is the total magnification of your microscope when the given objective is in place?
(1point each= 1x4= 4 points). Do not forget to put “x” beside the total magnification value. No
x=0.5 point.

Objective in Place Total Magnification

Scanner
Low Power Objective (LPO)
High Power Objective (HPO)
Oil Immersion Objective (OIO)

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Laboratory Worksheet 1: The Compound Microscope
Senior High School Department
2nd Period/ A.Y. 2023-2024
C. Magnification/Reduction = Size of the drawing/size of the object. (8 points total)

Specimen Actual Size Computation for the Is it magnified or

Magnification/Reduction of a reduced?
5cm drawing (1 point each)
(1 point each)
Hydrilla tooth cell 0.01mm
(diameter)

(1 point)
Length of a
potted plant
(Measure from the
part above the
soil to the tip)
(1 point)
Rice grain
(length)

Submission Note:
1. Save the document in x.pdf format with the file name:
Section_Group#_ ACTIVITY# (ex. STEM12A1_Group4_Activity05)

2. Upload the file to the corresponding module under the submission page in the CANVAS.

Table of Contributions: Indicate the work contribution of the group members below. Member with no
participation will be graded zero (0).

Name Contribution Remarks

1.
2.
3.
4.
5.

COPYRIGHT OF THIS MATERIAL (except video links) BELONGS TO:

Biology Department
De La Salle University

NO PART OF THIS MATERIAL MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM OR BY ANY MEANS --- ELECTRONICALLY OR
MECHANICALLY

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Laboratory Worksheet 1: The Compound Microscope
Senior High School Department
2nd Period/ A.Y. 2023-2024