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Introduction to Molecular
Spectroscopy Lecture 1
Lecturer Lecture Plan
Dr Simon Officer 1. Introduction to UV Molecular
Spectroscopy
Contact Information:
2. Instrumentation and Quantitative
Dr Simon Officer Analysis
N548 3. Introduction to Fluorescence
s.officer@rgu.ac.uk Spectroscopy

01224 262370 4. FTIR

5. Atomic Emission Spectrometry

6. Atomic Absorption Spectrometry

ER by Erin Ross
Last edited less than a minute ago
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 Lecture Plan
Introduction to UV Molecular Spectroscopy: This lecture provides an overview of
UV molecular spectroscopy, which involves the study of the interaction between
molecules and ultraviolet light. Topics covered include the principles of UV
spectroscopy and its applications in various fields.
Instrumentation and Quantitative Analysis: This lecture focuses on the instruments
used in UV spectroscopy and the techniques for quantitative analysis. Students will
learn about the components of a UV spectrophotometer and how to perform
quantitative measurements.
Introduction to Fluorescence Spectroscopy: In this lecture, students will be
introduced to fluorescence spectroscopy, which involves the study of the emission of
light by molecules after they absorb photons. The principles of fluorescence and its
applications in various fields will be discussed.
FTIR: This lecture introduces Fourier transform infrared spectroscopy (FTIR), a
technique used to obtain infrared spectra of samples. Students will learn about the
theory behind FTIR and its applications in various fields, including chemistry and
materials science.
Atomic Emission Spectrometry: This lecture focuses on atomic emission
spectrometry, a technique used to analyze the elemental composition of samples.
Students will learn about the principles of atomic emission and the instrumentation
used in this technique.
Atomic Absorption Spectrometry: In this lecture, students will learn about atomic
absorption spectrometry, a technique used to determine the concentration of
elements in a sample. The principles of atomic absorption and the instrumentation
used in this technique will be discussed.
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 Tutorial: Molecular Spectroscopy
Students will attend a tutorial session on molecular spectroscopy, where they will have
the opportunity to reinforce their understanding of the concepts covered in the lectures.
The tutorial will involve problem-solving exercises, discussions, and hands-on
experiments to enhance their knowledge and analytical skills in molecular spectroscopy.

Directed Study
Atomic Background
Interferences and
Background Correction
This section of directed study focuses on
atomic background interferences and
background correction techniques in
spectroscopy. Students will explore the
sources of interferences in atomic
spectroscopy and learn about various
methods to correct for them. The
importance of accurate background
correction in obtaining reliable analytical
results will be emphasized.
Mass Spectrometry (Part
1 and 2)
Students will delve into the principles
and applications of mass spectrometry in
two parts. In Part 1, they will learn about
the basic concepts of mass spectrometry,
including ionization techniques, mass
analyzers, and detectors. In Part 2, they
will explore the applications of mass
spectrometry in various fields, such as
drug discovery, forensics, and
environmental analysis.

Today's Topics
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 Today's Topics
1 Revision from AS1801 –
Chemistry for Life
Sciences
Students will revisit and revise key
concepts covered in the AS1801
Chemistry for Life Sciences course,
including the electromagnetic
spectrum, calculations involving
wavelength, energy, and
frequency, molecular transitions,
chromophores, visible light, and
absorption of light by dyes.
3 Molecular Orbitals and
Key Transitions
This section explores the concept
of molecular orbitals and their role
in UV spectroscopy. Students will
learn about the electrons involved
in UV absorptions, molecular
orbitals theory, bonding, anti-
bonding orbitals, and key
electronic transitions.
5 Effect of Solvent on Spectra
This topic focuses on the effect of solvent on the absorption spectra of organic
molecules. Students will explore how the polarity of solvents can influence the
position of absorption peaks in UV spectroscopy.
Revision
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2 Introduction to UV
Molecular Absorption
Spectroscopy
This topic provides an introduction
to UV molecular absorption
spectroscopy, which involves the
study of the interaction between
molecules and ultraviolet light.
Students will learn about the
principles of UV absorption
spectroscopy and its applications
in various fields.
4 Conjugation and
Auxochromes
Students will delve into the
concepts of conjugation and
auxochromes, which impact the
absorption spectra of organic
molecules. They will learn how
conjugated systems and
auxochromes influence the
wavelengths of light absorbed by
chromophores.
 Revision

Please refer to the slides on Moodle for revision on the following topics covered in
AS1801:

1 The Electromagnetic Spectrum

Students will revise the different regions of the electromagnetic spectrum and
their respective wavelengths, frequencies, and energies.

2 Calculations involving Wavelength, Energy, and Frequency

Students will refresh their understanding of the relationships between

wavelength, energy, and frequency through mathematical calculations.

3 Molecular Transitions
This section revisits the concept of molecular transitions, wherein electrons
undergo energy level changes and absorb or emit radiation.

4 Chromophores
Students will review the characteristics and properties of chromophores,
which are responsible for the absorbance of light in organic molecules.

5 Visible Light
The properties of visible light, including its range of wavelengths and colors,
will be revised.

6 Absorption of Light by Dyes

Students will revisit the absorption of light by dyes and understand the
principles behind the coloring of various substances.

If you have any questions, please feel free to ask.

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 The Absorption Process

1 Transmittance
The intensity of the beam is
decreased as some energy is
absorbed by analyte molecules.
Transmittance, represented as I/I0,
quantifies the fraction of the
incident light intensity (I) that
passes through the sample
compared to the initial light
intensity (I0).
2 Absorbance
Absorbance, represented as
log(I0/I), measures the amount of
light absorbed by the analyte. It is
commonly expressed as the
negative logarithm of the
transmittance
(log(1/Transmittance)).

3 Percent Transmittance

Transmittance is often expressed as a percentage (%T) by multiplying the

transmittance value by 100. For example, %T = (Transmittance) x 100.

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