Bio Technology - Tute 02

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Unit 18- Bio technology (II) - Complete the Structured Essay questions and develop the lesson note 1. 21). What is a "Bio - Reactor / Fermenter" ? BIO REACTOR 2. 22) State 4 examples for applications of "Bio reactor’ in bio-technology (4) 3 4, 23) Name the set up shown in the diagram below Questions (23-26) are based on the following diagram motor I Lt —fw x pH and temperature sensors water in => —+ water out [> stiring paddles 23) a. Identify what enters or leaves via W, X, Y and Z (one example each) (4) 5. 23)b. Identify the part labelled as "W" in the diagram above and state the purpose of W in the above set up (3) 6. 23) c. Identify the part labelled as "X" in the diagram above and state the purpose of X in the processes carried out by the above set up. (2) 7. 234). Name the inlet shown by "Y" explain the significance of having a broad plate at the inlet "Y" (3) 8. 23 e) Why are pH and temperature sensors used in the above set up? (2) 9. 23 £) Identify the letter representing the Cooling water jacket and briefly explain the purpose of having a cooling water jacket in the above set up (2) 10. 24) Having a rotating paddle stirrer ensures that the nutrients are evenly distributed within the set-up. Do you agree with the above statement? Explain. State another use of having a paddle stirrer in the above set-up (1) 11, 25)"Z"in the above equipment comprises of a tap. Briefly explain the importance of having a tap in the Z (2) 12. 26) Suggest one advantage of the motor being located outside, rather than inside, the reaction vessel shown in the diagram (1) 13, 27) How are biological washing powders synthesized ? Briefly explain how the enzymes used in this application can be helpful in removing stains (4) 14. 28) Observe the following diagram that shows the effect of using Pectinase enzyme on juice extraction. Fill in the statement underneath the diagram with ‘suitable words. (1) control and the test pectinase enzyme. Juice extracted without Pectinase ‘There is an extensive increase in the volume of the 15, 28a) Comment on the clarity of the juice extracted with/ without Pectinase and briefly explain the reason for the difference in clarity (2) 16. 29) Read the following diagram and briefly explain how Lactose- free milk is being produced (4) HOW Do WE MAKE Ir LACTOSE FREE? @ MILK contains natural sugar ealled LACTOSE, ‘WHAT IS LACTOSE? ‘Ourproduets ure made from sah ergaeteile Towtatt Tomake our products lactose free, we add the LACTASE ENZYME to our organis mile. &, om ES &-§ inga TWO DIGESTIBLE SUGARS: lactose Free, we add an enzyme called lactase that breaks down the lsetore (milk sugad) into two casily digestible sugars. Ifyou have lactose intolerance you donk have enough lsetase ‘enzymes to digest the suger in mill, and this can be really ‘uncomfortable. So,we do this little step for you. MADE WITH SIMPLE * INGREDIENTS GLUCOSE GALACTOSE Made with pure and simple ingredients, live and active soatteresiard Fxphimilieend cream Frem local family farms. / LacTOSE Frep Greet Valley 17. 29 a) How can lactose- free milk be useful to individuals who have encountered issues in milk digestion? (2) 18. 29 b) Briefly explain how the knowledge of microbes have been made into use in the production of lactose free milk 19. 30) What is meant by "Gene Modification" 20. 31) Fig. 4.1 shows the stages in the process of genetic engineering to produce the hormone insulin. What is the name given to the bacteria whose genome is altered to produce compounds which are useful to the human kind? stage K stage L a wee — DNA plasmid i | | sitet a— cuts the plasmid insulin gene inserted into the plasmid transformed plasmic inserted into bacterium to form transformed bacterium production of insulin Fig. 4.1 21. 32. Describe how the location and organization of genetic material in the human. cell shown in stage K of Fig. 4.1 is different from that in the bacterial cell shown (3) 22. 33. Briefly explain how the bacterial plasmid is altered genetically to be used as a vector in GMOs in Insulin production 23. 34. Use your knowledge of bacterial cells to name two structures that the transformed plasmid must pass through to form a transformed bacterium in stage L of Fig. 4.1. (2) 24. — 35. State the type of reproduction that takes place in stage M of Fig. 4.1. Use your knowledge of the process of cell division to explain why it is important that this type of reproduction occurs. (3) 25. 36. Stage N of Fig. 4.1 may take place in a container similar to that used in the large-scale production of antibiotics. State the name of this type of container 26. 37 Name the condition in humans that is treated using insulin produced by the bacteria in stage N of Fig. 4.1 27. Name the student This content is neither cieated nor endorsed by Google Google Forms

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