Unit 18- Bio technology (II)
- Complete the Structured Essay questions and develop the lesson note
1. 21). What is a "Bio - Reactor / Fermenter" ?
BIO
REACTOR
2. 22) State 4 examples for applications of "Bio reactor’ in bio-technology (4)3
4,
23) Name the set up shown in the diagram below Questions (23-26) are based on the
following diagram
motor I
Lt
—fw x
pH and
temperature
sensors
water in
=>
—+ water out
[> stiring
paddles
23) a. Identify what enters or leaves via W, X, Y and Z (one example each) (4)5. 23)b. Identify the part labelled as "W" in the diagram above and state the purpose of
W in the above set up (3)
6. 23) c. Identify the part labelled as "X" in the diagram above and state the purpose of
X in the processes carried out by the above set up. (2)
7. 234). Name the inlet shown by "Y" explain the significance of having a broad plate at
the inlet "Y" (3)
8. 23 e) Why are pH and temperature sensors used in the above set up? (2)9. 23 £) Identify the letter representing the Cooling water jacket and briefly explain the
purpose of having a cooling water jacket in the above set up (2)
10. 24) Having a rotating paddle stirrer ensures that the nutrients are evenly distributed
within the set-up. Do you agree with the above statement? Explain. State another
use of having a paddle stirrer in the above set-up (1)
11, 25)"Z"in the above equipment comprises of a tap. Briefly explain the importance
of having a tap in the Z (2)12. 26) Suggest one advantage of the motor being located outside, rather than inside,
the reaction vessel shown in the diagram (1)
13, 27) How are biological washing powders synthesized ? Briefly explain how the
enzymes used in this application can be helpful in removing stains (4)14. 28) Observe the following diagram that shows the effect of using Pectinase
enzyme on juice extraction. Fill in the statement underneath the diagram with
‘suitable words. (1)
control and the test pectinase enzyme.
Juice extracted
without Pectinase
‘There is an extensive increase in the volume of the
15, 28a) Comment on the clarity of the juice extracted with/ without Pectinase and
briefly explain the reason for the difference in clarity (2)16. 29) Read the following diagram and briefly explain how Lactose- free milk is being
produced (4)
HOW Do WE MAKE Ir
LACTOSE FREE?
@
MILK contains natural
sugar ealled LACTOSE,
‘WHAT IS LACTOSE?
‘Ourproduets ure made from
sah ergaeteile Towtatt Tomake our products lactose
free, we add the LACTASE
ENZYME to our organis mile.
&, om
ES &-§
inga TWO DIGESTIBLE
SUGARS:
lactose Free, we add an enzyme
called lactase that breaks down
the lsetore (milk sugad) into two
casily digestible sugars. Ifyou
have lactose intolerance you
donk have enough lsetase
‘enzymes to digest the suger in
mill, and this can be really
‘uncomfortable. So,we do this
little step for you.
MADE WITH SIMPLE *
INGREDIENTS GLUCOSE GALACTOSE
Made with pure and simple
ingredients, live and active
soatteresiard Fxphimilieend
cream Frem local family farms.
/
LacTOSE Frep
Greet Valley17. 29 a) How can lactose- free milk be useful to individuals who have encountered
issues in milk digestion? (2)
18. 29 b) Briefly explain how the knowledge of microbes have been made into use in
the production of lactose free milk19. 30) What is meant by "Gene Modification"20. 31) Fig. 4.1 shows the stages in the process of genetic engineering to produce the
hormone insulin. What is the name given to the bacteria whose genome is altered to
produce compounds which are useful to the human kind?
stage K
stage L
a wee
—
DNA plasmid
i |
| sitet
a— cuts the plasmid
insulin gene inserted into
the plasmid
transformed plasmic
inserted into bacterium to
form transformed bacterium
production of insulin
Fig. 4.121. 32. Describe how the location and organization of genetic material in the human.
cell shown in stage K of Fig. 4.1 is different from that in the bacterial cell shown (3)
22. 33. Briefly explain how the bacterial plasmid is altered genetically to be used as a
vector in GMOs in Insulin production
23. 34. Use your knowledge of bacterial cells to name two structures that the
transformed plasmid must pass through to form a transformed bacterium in stage
L of Fig. 4.1. (2)24. — 35. State the type of reproduction that takes place in stage M of Fig. 4.1. Use your
knowledge of the process of cell division to explain why it is important that this
type of reproduction occurs. (3)
25. 36. Stage N of Fig. 4.1 may take place in a container similar to that used in the
large-scale production of antibiotics. State the name of this type of container
26. 37 Name the condition in humans that is treated using insulin produced by the
bacteria in stage N of Fig. 4.1
27. Name the student
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