UJIAN AMALI TINGKATAN 4 2023

BIOLOGY: CHAPTER 3 MOVEMENT OF SUBSTANCE ACROSS THE PLASMA MEMBRANE

Experiment 3.1: Movement of substances across a selectively permeable membrane using Visking Tubing
Aim : To study the movement of substances across a selectively permeable membrane
Problem statement : Does the size of dissolved particles affect the movement of substances across a selectively permeable.
membrane?
Hypothesis : Only small molecules are able to diffuse through a selectively permeable membrane while a large molecule
cannot diffuse through a selectively permeable membrane.
Variables: -
- Manipulated : Size of molecules
- Responding : Change in colour of solution in Visking tubing and beaker
- Constant : Surrounding temperature and soaking time

Materials : Benedict’s solution, 1% starch suspension, iodine solution, 30% glucose solution, distilled water, Visking
tubing, (12cm) and thread.
Apparatus : Test tubes, beaker, dropper, measuring cylinder and Bunsen burner

Procedure: -
1. Soak the Visking tubing in water for 5 minutes to soften it. Make a knot and tie one end of the Visking tubing with thread to
prevent leaking
2. Fill the Visking tubing with 10ml glucose solution and 10ml starch suspension. Tie one end of the Visking tubing tightly with a
thread. The solution colour is recorded.
3. Rinse the outside of the Visking tubing with distilled water.
4. Add 400ml of distilled water in a beaker.
5. Place the Visking tubing in the beaker and leave it for 30 minutes.
6. After 30 minutes, remove and transfer the Visking tubing into a dry beaker.
7.1 Conduct the iodine test for the solution in the Visking tubing and in the beaker. Put 2ml of each solution in separate test tubes
and add 1ml of the iodine solution. Observe the colour produced.
 7.2 Conduct the Benedict’s test on the solution in the Visking tubing and in the beaker. Put 2ml of each solution into separate test
tubes and add 1ml of Benedict’s solution. Heat the solutions in a water bath for 5 minutes and record any changes in colour.

Results
Contents Iodine test Benedict’s test
Visking tubing 10ml glucose solution + DARK BLUE BRICK RED
10ml starch solution PRECIPITATE
Beaker 400ml distilled water BROWN RED

Discussion:
1. What molecules is found in (a) Visking tubing (b) Beaker?
(a) Glucose and starch
(b) Glucose

2. What are the inference that can be made based on the (a) size of the starch molecule (b) size of the glucose molecule compared
to the pore size in the Visking tubing?
Glucose is a small molecule compare to the pore size in the Visking tubing therefore it is able to diffuse through selectively
permeable membrane however size of starch is bigger compared to the pore size of the Visking tubing.

3. What are the similarities between a Visking tubing and a plasa membrane?
Both Visking tubing and plasma membrane able to allow diffusion// transport small molecules
CONTOH SOALAN: PEPERIKSAAN PERCUBAAN SIJJIL PELAJARAN MALAYSIA (PPSPM) SBP 2023
1. A pupil repeats this experiment by replacing Solution P (glucose + starch solution) with Juice X into the Visking tubing.

Predict the colour changes in the Visking tubing after 15 minutes.

Prediction:
No changes in colour

Explanation:
P1 – Juice X contains sucrose.
P2 – Sucrose is a disaccharide.
P3 – Iodine will not react with disaccharide/sucrose // iodine only react with polysaccharide/starch.
Conclusion:
The hypothesis is accepted. Small molecules are able to diffuse through a selectively permeable membrane while large molecules
cannot diffuse through a selectively permeable membrane.