Standard Operative Procedures in Histopathology For Laboratory Technicians

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STANDARD OPERATIVE

PROCEDURES IN HISTOPATHOLOGY

FOR LABORATORY TECHNICIANS

DR. DEEPIKA K. HEMRAJANI


(SMS Medical College, Jaipur)
STANDARD OPERATIVE

PROCEDURES IN HISTOPATHOLOGY

FOR LABORATORY TECHNICIANS


TRAINING SCHEDULE

Day Session - I Session - II Session - III Session-IV

1 Introduction to Fixation Types of fixative Exercise / Evaluation by


Histopathology & Aims Choice of fixative assessment
Responsibility of the Theories & Washing out &
technician. Collection & Principle Post chromatization
record keeping

2 Decalcification Treatment of Removal of pigments Exercise / Evaluation by


Aims, Principle & Hard tissues Formalin, Melanin, assessment
Assessment of end point Picricacid & Mercury
of decalcification

3 Tissue processing Casting Automatic tissue Exercise / Evaluation by


Dehydration clearing & processor & processing assessment
impregnation schedule

4 Types of knives, Parts of machine & hints Trouble shooters in Exercise / Evaluation by
microtomes & microtomy on section cutting section cutting assessment

5 Basic terminology used in Types of mountants & H&E staining principle Exercise / Evaluation by
staining and Theories of types of haematoxylin procedure & causes of assessment
staining poor staining

6. Special staining Special staining Special staining Exercise / Evaluation by


(connective tissue) (PAS & Mucicarmine AFB) for iron, calcium & bilirubin assessment

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