. 2015. 1. . 95–100.

DOI: 10.14258/jcprm.201501410

615.32:547.9+543.544

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genia crassifolia (L.) Fitsch.), // . 2003. 1. . 73–77.
6. Ph.Eur. 2012: European Pharmacopoeia, 8 th edition. Strasbourg (FR): Directorate for the Quality of Medicines and
HealthCare of the Council of Europe (EDQM), 2012. Pp. 1162–1163.
7. Uva Ursi Leaf (Arctostaphylos uva-ursi (L.) Spreng). Standards of analysis, quality control, and therapeutic // Ameri-
can Herbal Pharmacopoeia. 2008.
8. . . / .: . , . . ., 2013. . 316–319.
1 2014 .

11 2014 .
100 . , . , . , .

Kurkin V.A.1*, Ryazanova T.K.1, Platonov I.A.2, Pavlova L.V.2 QUANTITATIVE DETERMINATION OF ARBUTIN
IN THE LEAVES OF ARCTOSTAPHYLOS UVA-URSI (L.) SPRENG.
1
Samara State Medical University, Chapaevskaia st., 89, Samara, 443099 (Russia), e-mail: Kurkinvladimir@yandex.ru
2
Samara State Aerospace University named Academician S.P. Korolev, Moskovskoe shosse, 34, Samara, 443086 (Rus-
sia)
The method of quantitative determination of arbutin in leaves of bearberry (Arctostaphylos uva-ursi (L.) Spreng.) using
reverse-phase HPLC and justified optimal conditions of extraction of arbutin from raw materials of plants (extractant – 40%
ethyl alcohol; the extraction time – 60 min a boiling water bath; the ratio of «raw material : extractant» – 1 : 50). Conditions of
carrying out of the analysis: the mobile phase 0,01 M KH2PO4 acidified 3 4 to pH 3,00±of 0,01, and acetonitrile (9 : 1); the
flow of the mobile phase: 0,6 ml/min; detection at 280 nm. It is determined that the error of a single definition of the contents of
arbutin in leaves of bearberry with confidence probability of 95% is +3,14%. The content of arbutin in leaves of bearberry var-
ies from 10,85±0,24% to 11,16±0,35%.
Keywords: bearberry, Arctostaphylos uva-ursi (L.) Spreng., leaves, arbutin, HPLC-analysis, spectrophotometry, iodime-
try, standardization.
References
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HealthCare of the Council of Europe (EDQM), 2012. Pp. 1162–1163.
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Received July 1, 2014

Revised October 11, 2014

*
Corresponding author.