Saraswati Vidhya Mandir, Gandhinagar

Chapters : 1,2 Std 12 : Biology Date : 07/03/24

Total Marks : 30 Unit Test Time : 03 Hour
Section A
//X
• Choose correct answer from the given options. [Each carries 1 Mark] [30]

1)
1. Antibiotics inhibit the growth of or destroy
(A) Bacteria and fungi (B) Bacteria and viruses
(C) Bacteria, algae and viruses (D) Bacteria, fungi and viruses
2)
2. Broad spectrum antibiotic is that which
(A) Acts on both pathogens and hosts
(B) Acts on all bacteria and viruses
(C) Acts on a variety of pathogenic micro-organisms
(D) Is effective in very small amounts
3)
3. The sequence that controls the copy number of the linked DNA in the vector, is termed
(A) Recognition site (B) Selectable marker (C) Ori site (D) Palindromic sequence
4)
4. Use of living organisms or their substances in industrial processes is called
(A) Microbiology (B) Biotechnology (C) Industrial engineering (D) Genetic engineering
5)
5. Definition of biotechnology was given by .....
(A) American federation of biotechnology (B) European federation of biotechnology
(C) Australian federation of biotechnology (D) Indian federation of biotechnology
6)
6. Assertion : Agrobacterium tumefaciens is popular in genetic engineering be-cause this bacterium is associated
with the roots of all cereal and pulse crops.
Reason : A gene incorporated in the bacterial chromosomal genome-gets auto-matically transferred to the
crop with which the bacterium is associated.
(A) Both Assertion and Reason are correct and the Reason is a correct ex-planation of the Assertion.
(B) Both Assertion and Reason are correct but Reason is not a correct expla-nation of the Assertion.
(C) The Assertion is correct but Reason is incorrect.
(D) Both the Assertion and Reason are incorrect.
7)
7. Match the column - I with column - II with respect to the nomenclature of enzyme Eco RI and select the
correct answer from codes given below.
Column −I Column−II
AE (1) 1st in order of identificaiton
B Co (2) Name of genus
CR (3) Name of species
DI (4) Name of strain
(A) A − 3, B − 4, C − 1, D − 2 (B) A − 2, B − 3, C − 4, D − 1
(C) A − 2, B − 1, C − 4, D − 3 (D) A − 2, B − 3, C − 1, D − 4
8)
8. It is called sticky ends.
(A) Single stranded part of DNA. (B) Double stranded part of DNA.
(C) Over hanging single stranded part DNA (D) Over hanging double stranded part of DNA
9)
9. Restriction endonucleases
(A) Are used for in vitro DNA synthesis
(B) Are synthesized by bacteria as partWish You - All
of defense The Best
mechanism
(C) Are present in mammalian cells for degradation of DNA when the cells dies
(D) Are used in genetic engineering for ligating two DNA molecules
9) Restriction endonucleases
(A) Are used for in vitro DNA synthesis
(B) Are synthesized by bacteria as part of defense mechanism
(C) Are present in mammalian cells for degradation of DNA when the cells dies
(D) Are used in genetic engineering for ligating two DNA molecules
10)
10. In Gene cloning :
(A) Gene is isolated and inserted in same organism
(B) Gene is isolated and inserted in different organism
(C) Gene is isolated and inserted in plasmid of other organism
(D) Gene is isolated and inserted in chromosomal DNA
11)
11. Identify the wrong statement with regard to Restriction Enzymes.
(A) Sticky ends can be joined by using DNA ligases.
(B) Each restriction enzyme functions by inspecting the length of a DNA se-quence.
(C) They cut the strand of DNA at palindromic sites.
(D) They are useful in genetic engineering.
12)
12. The term genetic engineering is used for
(A) Blotting technique (B) RNA reaction technique
(C) Protein synthesis technique (D) Recombinant DNA technique
13)
13. Plasmid of which bacterium was first time used in recombinant DNA technology?
(A) E.coli (B) Salmonella typhimurium
(C) Haemophilus influenzae (D) Streptococcus pneumoniae
14)
14. An alien DNA is linked with the origin of replication, so that this alien piece of DNA can replicate and
multiply itself in the host organism This can also be called ......
(A) Tissue culture (B) Cloning (C) Splicing (D) Mutation
15)
15. Restriction endonuclease, an enzyme used in Genetic Engineering is employed for
(A) Probing exons (B) Cutting double stranded DNA
(C) Cutting single stranded DNA (D) Join strands of DNA
16)
16. Recombinant DNA technology was discovered by
(A) Khorana (B) Bateson and de Vries (C) Watson (D) Cohen and Boyer
17)
17. I. Copy number is defined as the number of copies of plasmid present in a cell.
II. It varies from 15 − 100 copies per cell.
Choose regarding the above statements.
(A) I is true, Il is false (B) II is true, I is false (C) Both are true (D) Both are false
18)
18. Which enzyme is used as molecular scissors ?
(A) Proteases (B) Ligase
(C) Restriction Endonuclease (D) Liases
19)
19. ........ make cuts at specific positions within the DNA.
(A) DNA ligase (B) Restriction endonuclease
(C) DNA polymerase (D) Reverse transcriptase
20)
20. Restriction enzymes are also called
(A) Molecular markers (B) Vectors (C) Carriers (D) Molecular scissors
21)
21. Recombinant DNA technology is related with
(A) C. Darwin (B) Stanley Cohen (C) Herbert Boyer (D) Both (B) and (C)
22)
22. Which of the following is used in biotechnology
(A) Cattle (B) Yeast
(C) Both cattle and yeast Wish You - All(D)
TheNeither
Best cattle nor yeast
22) Which of the following is used in biotechnology
(A) Cattle (B) Yeast
(C) Both cattle and yeast (D) Neither cattle nor yeast
23)
23. Special sequence from where DNA replications starts.
(A) Selectable marker (B) Origin of replication
(C) Sequence of replication (D) Recognition site
24)
24. It increases the efficiency with which DNA enters the bacterium through pores in its cell wall.
(A) PO−4 (B) CI− (C) Ca++ (D) Na++
25)
25. In gel electrophoresis, the separated bands of DNA are cut out and extracted from the gel piece. This step
is called
(A) Elution (C) Competency (B) Origin replication (D) Transformation
26)
26. Which of the following components are used in gel electrophoresis ?
I. Ethidium bromide
II. Restriction endonuclease
III. Agarose
IV. UV radiation
Choose the correct option :
(A) I and II only (B) I and III only (C) I, II and IV only (D) I, II, III and IV
27)
27. What is true about DNA polymerase used in PCR ?
(A) It is used to ligate introduced DNA inrecipient cells.I
(B) It serves as selectable marker.
(C) It is isolated from virus.
(D) It is thermostable enzyme.
28)
28. Choose the correct option
A Cla I Hind III
Pvu I
Pst I B
C tetR
pBR322 Sal I

D
rop

Pvu II
(A) A → Hind IB → EcoR I C → ampR D → ori (B) A → Hind IB → Bam HI C → kanR D → ampR
(C) A → Bam HI B → Pst IC → ori D → ampR (D) A → EcoR I B → Bam HI C → ampR D → ori
29)
29. Genes of interest can be selected from a genomic library by using
(A) Restriction enzymes (B) Cloning vectors (C) DNA probes (D) Gene targets
30)
30. Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes)
or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population, which express the desired
gene.
(A) Both Assertion and Reason are correct and the Reason is a correct ex-planation of the Assertion.
(B) Both Assertion and Reason are correct but Reason is not a correct expla-nation of the Assertion.
(C) The Assertion is correct but Reason is incorrect.
(D) Both the Assertion and Reason are incorrect.
Wish You - All The Best
(B) Both Assertion and Reason are correct but Reason is not a correct expla-nation of the Assertion.
(C) The Assertion is correct but Reason is incorrect.
(D) Both the Assertion and Reason are incorrect.

Wish You - All The Best

 Saraswati Vidhya Mandir, Gandhinagar
Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 03 Hour

Section [ A ] : 1 Mark MCQ

No Ans Chap Sec Que Universal_QueId

1. D Chap 9 S4 1 QP23P13B1214_P2C9S4Q1
2. C Chap 9 S4 2 QP23P13B1214_P2C9S4Q2
3. C Chap 9 S4 3 QP23P13B1214_P2C9S4Q3
4. B Chap 9 S4 4 QP23P13B1214_P2C9S4Q4
5. B Chap 9 S4 5 QP23P13B1214_P2C9S4Q5
6. D Chap 9 S4 6 QP23P13B1214_P2C9S4Q6
7. B Chap 9 S4 7 QP23P13B1214_P2C9S4Q7
8. C Chap 9 S4 8 QP23P13B1214_P2C9S4Q8
9. B Chap 9 S4 9 QP23P13B1214_P2C9S4Q9
10. B Chap 9 S4 10 QP23P13B1214_P2C9S4Q10
11. A Chap 9 S4 11 QP23P13B1214_P2C9S4Q11
12. D Chap 9 S4 12 QP23P13B1214_P2C9S4Q12
13. B Chap 9 S4 13 QP23P13B1214_P2C9S4Q13
14. B Chap 9 S4 14 QP23P13B1214_P2C9S4Q14
15. B Chap 9 S4 15 QP23P13B1214_P2C9S4Q15
16. D Chap 9 S4 16 QP23P13B1214_P2C9S4Q16
17. C Chap 9 S4 17 QP23P13B1214_P2C9S4Q17
18. C Chap 9 S4 18 QP23P13B1214_P2C9S4Q18
19. B Chap 9 S4 19 QP23P13B1214_P2C9S4Q19
20. D Chap 9 S4 20 QP23P13B1214_P2C9S4Q20
21. D Chap 9 S4 21 QP23P13B1214_P2C9S4Q21
22. C Chap 9 S4 22 QP23P13B1214_P2C9S4Q22
23. B Chap 9 S4 23 QP23P13B1214_P2C9S4Q23
24. C Chap 9 S4 24 QP23P13B1214_P2C9S4Q24
25. A Chap 9 S4 25 QP23P13B1214_P2C9S4Q25
26. D Chap 9 S4 26 QP23P13B1214_P2C9S4Q26
27. D Chap 9 S4 27 QP23P13B1214_P2C9S4Q27
28. D Chap 9 S4 28 QP23P13B1214_P2C9S4Q28
29. C Chap 9 S4 29 QP23P13B1214_P2C9S4Q29
30. A Chap 9 S4 30 QP23P13B1214_P2C9S4Q30

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 Saraswati Vidhya Mandir, Gandhinagar
Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 01 Hour

Student Name : _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Roll Number : 0 1 2 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

A B C D A B C D A B C D
1. 11. 21.
2. 12. 22.
3. 13. 23.
4. 14. 24.
5. 15. 25.
6. 16. 26.
7. 17. 27.
8. 18. 28.
9. 19. 29.
10. 20. 30.

Saraswati Vidhya Mandir, Gandhinagar

Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 01 Hour

Student Name : _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Roll Number : 0 1 2 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

A B C D A B C D A B C D
1. 11. 21.
2. 12. 22.
3. 13. 23.
4. 14. 24.
5. 15. 25.
6. 16. 26.
7. 17. 27.
8. 18. 28.
9. 19. 29.
10. 20. 30.
 Saraswati Vidhya Mandir, Gandhinagar
Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 01 Hour

OMR ANSWER SHEET

Marks Identifier :

A B C D A B C D A B C D
1. 11. 21.
2. 12. 22.
3. 13. 23.
4. 14. 24.
5. 15. 25.
6. 16. 26.
7. 17. 27.
8. 18. 28.
9. 19. 29.
10. 20. 30.

Saraswati Vidhya Mandir, Gandhinagar

Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 01 Hour

OMR ANSWER SHEET

Marks Identifier :

A B C D A B C D A B C D
1. 11. 21.
2. 12. 22.
3. 13. 23.
4. 14. 24.
5. 15. 25.
6. 16. 26.
7. 17. 27.
8. 18. 28.
9. 19. 29.
10. 20. 30.
 Saraswati Vidhya Mandir, Gandhinagar
Chapters : 1,2 Std 12 : Biology Date : 07/03/24
Total Marks : 30 Unit Test Time : 03 Hour
Section A
//X
• Choose correct answer from the given options. [Each carries 1 Mark] [30]

1.

//X
 Ans : (D)
2.

//X
 Ans : (C)
3.

//X
 Ans : (C)
4.

//X
 Ans : (B)
5.

//X
 Ans : (B)
6.

//X
 Ans : (D)
7.

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//X
 Ans : (B)
8.

//X
 Ans : (C)
9.

//X
 Ans : (B)
10.

//X
 Ans : (B)
11.

//X
 Ans : (A)
12.

//X
 Ans : (D)
13.

//X
 Ans : (B)
14.

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//X
 Ans : (B)
15.

//X
 Ans : (B)
16.

//X
 Ans : (D)
17.

//X
 Ans : (C)
18.

//X
 Ans : (C)
19.

//X
 Ans : (B)
20.

//X
 Ans : (D)
21.

//X
 Ans : (D)
22.

//X
 Ans : (C)
23.

//X
 Ans : (B)
24.

//X
 Ans : (C)
25.

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//X
 Ans : (A)
26.

//X
 Ans : (D)
27.

//X
 Ans : (D)
28.

//X
 Ans : (D)
29.

//X
 Ans : (C)
30.

//X
 Ans : (A)

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