ECOSHEL

CO2 Incubator
(Water-coat)

User Manual

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Ⅰ.Application Range:

The CO2 Cell Incubator provide modern medicine, medicine, biochemistry

and agricultural science research and industrial production departments of

biological cells, tissues, bacterial culture used.

II.Feature

1.This product is made up of high quality thermal insulation materials adopted

water jacket structure , with air duct liner and fan forced convection formed to

raise the box temperature uniformity and the equilibrium concentration of CO2。

2.Imported first-class CO2 detected by the infrared waveguide patents and gold-

plated probe.To ensure the accuracy of measurement data, sensor life of up to 15

years.

3.Working indoor CO2 concentrations can range from 0 to 20% of the arbitrary,

and in order to prevent pollution, reduce the concentration of CO2 gas in the

impurity effect.Work to improve the measurement accuracy and cleanliness of the

room, the equipment used within the loop gas filter.

4.When the door is open, automatically turn off fans and CO2 into the valve,

and stop heating.Savings of CO2 gas consumption, and isolated to reduce air

pollution caused by the entry.

5.Temperature and CO2 concentration in the use of computer data analysis

and intelligent PID control.High precision, strong anti-interference.Temperature

control which uses two probes, respectively, the door control box mild
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temperature, so that studio temperature high precision, small fluctuations.

6.Slight-touched adjustment switch, light and flexible.

7.Independent temperature control door, reduce the external temperature

thermostat on the system of studio and glass doors to prevent frost.

8.Natural evaporation humidifier, so that work to maintain good indoor humidity.

9.Box temperature, carbon dioxide concentration and the set of parameters, digital

display. Door heat, carbon dioxide into the air, high-water level, water shortage

with LED instruction.

10.Super warm, breathe and other protection features to ensure safe operation of

equipment

III.Main Technical Data

Model CI-80 CI-160
Capacity L 80 160
Temperature range ℃ RT+5～60
Temperature fluctuation ℃ ≤±0.2
Temperature uniformity ℃ ≤±0.3
Timing range （1～9999）min or no timing function
Air ml/min 160～1600
Flow
CO2 ml/min 10～100
Power ～220V 50Hz
Rated power W 600 900
Working chamber size （cm3） 41×40.3×51 50.5×45.3×70
Overall size （cm3） 57×65.5×88.5 68.5×69.5×107.5
N.W/G.W kg 60/90 80/115

Ⅳ.Installation

1.The equipment should be placed in a dry, flat, non-toxic and hazardous gas areas.

And should avoid direct sunlight, there is a certain space around equipment to

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facilitate maintenance.

2.Use of this equipment must be equipped with 99.9% of high purity gas cylinders,

and CO2 loaded valve (above components by users themselves). Cylinders should

be placed in the incubator near the incubator with silicone hoses and the rear "of

CO2 into the air intake" connected.

3.Incubator at ambient temperature (20 ~ 25) ℃ for the best. Incubator used to set

a minimum temperature of at least higher than the ambient temperature 5 ℃.

Ⅴ.Operating instructions of temperature controller

1.Panel diagram（Equipped instruments，CO2 temperature controller)

High Water
Door Pump2
Power Heat Overtemp Pump1
water Shortage Sterilize Light
Heat

Inner temperature

Pump1 Pump2 Sterilize Light

2.On the right of the panel is a set of keys, the functions are as follows：

1) Key : This key is a function key,after powered on, press this button can
enter the temperature setting state;Press this button for five seconds can enter

the other parameters setting state;When the instrument into the set state, the

bottom of LED will flashing

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2)"pump1 "Key: press this key to connect to pump1, press again to disconnect it.

3）"pump2 "Key:press this key to connect to pump2 , press again to disconnect

it.

4)▲Key:The key is the addend key. When setting parameters, press the button

to plus 1 .

5)▼Key:The key is the subtraction key. When setting parameters, press the key

minus 1.

6)◄Key：The key is the left key. When setting parameters, the key can moved to

set data bit. In the boot state, click the button, you can see the indoor temperature.

Note: Please press the key" " after setting parameters, or the parameters can
not been written to memory.

Instructions:

1)When running ， the temperature window (top row) shows the inside

temperature.Time window (bottom row) shows remaining time (when time set to

zero, the time window shows set temperature), when the remaining time reaches

0, it shows end， and will stop running.When it stopped running, press the key"

" to rerun.

2)Set inside temperature and run time as follows:①Press the key,the top

row digital display temperature settings,end-tern is blinks → ②Use ▼、▲、◄

modify status →③press the key" " ,the bottom row digital display the set

time→④Use ▼、▲、◄ modify time status →⑤Good parameters by key

parameter to write into memory,exit the setting state,return to running.

3)Set other parameters as follows:①Press the

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 ， the bottom row digital display parameter settings,end-term use in

flashing state → ②Use ▼、▲、◄ modify time status →③press the key"

"to write into memory, and adjust out the next parameter, and so on →④After

setup is complete,press the key for about 5 minutes, exit the setting state,
return to running.

4)Set the PID parameters as follows:Press the key for about 5 minutes,enter
the other parameter setting state first,then press ◄ key for about 5 minutes, enter

PID parameter setting state, the top row digital display each parameters symbols，

the bottom row digital display PID parameters,end-term use in flashing state →

②Use ▼、▲、◄ modify time status →③press the key" "to write into

memory, and adjust out the next parameter, and so on →④After setup is

complete,press the key for about 5 minutes, exit the setting state, return to
running.

5)Check indoor temperature:When running,press ◄ key, the temperature window

shows the inside temperature,three LED flash simultaneously,press ◄ key

again,return to show the inside temperature.

Definition and instructions of other parameter symbol :
Prompt Setting Range Instruction Factory value

AL 0～10.0℃ Overtemp Alarm 1.0℃

SC -10.0～10.0℃ Measurement error Random

Td -10.0～10.0℃ Measurement error correction Random

TS 0～10.0℃ Temperature difference between Random

the door and the box
TT 0～300S Temperature difference between Random

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 the water and the box

Definition and instructions of PID parameters:

Prompt Setting range Instruction Factory value

P1 0～50.0℃ Ratio of inner temperature previous Random
control
I1 1～2500S Inner temp integral time Random
D1 1～1000S Inner temp differential time Random
P2 0～50.0℃ Ratio of door temperature previous Random
control
I2 1～2500S Door temp integral time Random
D2 1～1000S Door temp differential time Random

1. Wiring diagram：

100Ω
Water Temp

Inner Temp Indoor Temp

Display door switch

COM
water shortage

high water
Main board
Water coat
1# 2# Fan Light Door Heat Steri
Pump Pump Heat lamp

1 2 3 4 5 6 7 8 9 10 11 12
Transformer

220VAC

2. Attention：
1) It will display ER1 if the inner probe is broken circuit or short circuit，ER2
for inner door broken circuit or short circuit，ER3 for short circuit or broken

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 circuit for the probe in the water.ER4 for machine beeping.
2) If the tested temp>setting temp ＋ AL ， the machine will beep to alarm
continuously，if the tested temp<setting temp＋temp alarm value, the machine
will beep to alarm, press any key to mute.
VI.Order of operations of using
1.Irrigation: Connect one end of the rubber tube to the "inlet，outlet" valve
behind the device，and the other end connected to water, open the "inlet ，
outlet 'valve. Put water into the water jacket by the water pressure. When
injected water is more than 12 liters (the water level exceed the heating tubes), it
can be connected to power, continue to inject water and observe the water level
indicator (water shortage was likely to alarm) until the high water level
indicator---the green light on，stop inject water, turn off "into , outlet "valve .
Or directly to inject water into the overflow until the water overflow, then
release a little water.
2.Please inject pure water or distilled water to prevent scaling inside the water
jacket.
3.Compounding PH liquid and detergent：
Please insert the PH bottle and the detergent bottle.
A．Detergent-The copper sulfate solution
CuSO2 / 5H2O 0.7～1.4g
H2SO4 0.25ml
Musk grass powder （few）
Stilled Water 500ml
B．PH liquid phenol red liquid
According to the room temperature, the solution should be changed once every
two weeks.
Stilled Water 1000ml
0.2%phenol red liquid 3ml

NaHCO3Reference Table
RT(℃) 14 16 18 20 22 24 26

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 NaHCO3(g) 1.87 1.80 1.73 1.67 1.61 1.54 1.48

RT(℃) 28 30 32 34 36 37 38

NaHCO3(g) 1.43 1.38 1.33 1.28 1.23 1.21 1.18

VII. Order of operations of using

Follow the instruction after installation and watering.

1.Open the door, make the working chamber clean and put the shelves well.

2.Please inject pure water or distilled water to prevent scaling inside the water

jacket.

3.After installed the incubator and irrigated water, you can operate according to

the following steps:

1)Open the door, clean the studio, put away the shelf.

2)If need humidity, humidity tray will need to join two-thirds of the water, place

the bottom in the studio, closing the door.

3)Open the power switch behind the control box, this time incubator should

display temperature, and high-water level indicator light on.

4)Press the "sterilize" key, sterile the studio. (Note: when there are cultures inside,

do not press this key!!) After sterilization, press "sterilization" button again, close

the germicidal lamp.

5)First of all, setting the required temperature for heating of the incubator. (See

the Ⅴ, the temperature controller operating instructions.)

6)Wait until the temperature constant, you can open the door and put the culture

into the box.

7)Press “CO2 /power”button，Turn on the CO2 control power supply.

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8)First, set up the required percentage of CO2 concentration. (See VI

concentration controller operating instructions)

9)Open the switch of the CO2 gas cylinder, adjust the CO2 valve slowly so that

the second pressure gauge indicates about 0.025~0.05Mpa，if the pressure is too

large and the CO2 concentrations rise too fast, it will increase the fluctuation range.

If the pressure is too small and the CO2 concentrations rise too slowly, it will

cause an alarm. Open the regulator valve (clockwise to open) general, it was

opened before it was sent out. Concentration can be seen gradually increased

after 1min, it can reach 5.0% after 10min (when the set value is 5.0%).

10)When working, if it requires cleanliness, you can open pump 1 or pump 2

(alternate use) for the inner loop filter.

4.When the incubator stops working, please click these steps:

1)Close the switch of the CO2 gas cylinder and the reducing valve.

2)Close the CO2 control power and the pump power to make the CO2 control

panel and pump stop working.

3)Open the door, remove the humidity tray. And to withstand the door switch by

hand to make the situation open the door to work in the incubator a few minutes

to disperse water vapor box.

4)Close the door and continue heating about 10min, then turn off the power and
clean inside.
VIII.Attention
1. Equipment should be installed in a clean place where has small changes
in temperature as possible.
2. Before powering on should be fully familiar and understand the
component parts of the manual, to master the correct use.
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 4. When the Incubator has no water, it can not boot, so as not to damage
the internal parts.

6. Turn the power off when exchanging the lamp .

7. Please do not randomly adjust the regulator valve behind the control
box, so as not to affect the CO2 concentration control. When the CO2
concentration increased too slowly or too quickly ,you can adjust
regulator valve, clockwise to increase, counterclockwise to decrease.
8. Connected CO2 gas cylinder to incubator inlet with a hose, and
fastened to prevent leakage. Second Reducing valve adjust in (0.05)
MPa and the maximum is not greater than 0.1Mpa.
9. If caisson pressure of CO2 cylinder is too low to generate the flow
unstable, it should be reversed or re-inflated before use.
10. To save energy and extend lamp's life, "light" switch can switch at any
time as needed.

12. In order to reduce temperature fluctuations and the uniformity after the
studio closed, so need to delay about 10min after closed (delay time can
be set according to actual by yourself), when it starts to heat up, please
do not frequently open the door!
13. Please exhaust the water in the water jacket when unused for long
periods .
IX.Troubleshooting
Phenomenon Reason Treatment

1．Power Alarm Sensor broken See phenomenon 2；

Continue to inject water to until high water

Water is not filled
level lights on

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 2．Box Temp
ER1 Bad sensor or open studio Check wiring or replace sensor.
Display
Bad sensor or open circuit
ER2 Check wiring or replace sensor.
within the door
Temperature sensor is
ER3 Check wiring or replace sensor.
broken or open circuit
the water level wiring short
ER4 Check wiring and measuring probe.
circuit or disconnect
Plug is not plugged in or
3.No Power Plugged in plug or connected line.
disconnected.
Fuse open Replace the same model fuse
4.Not Heating Set the temperature lower Adjust and set temperature

Door not properly closed or Close the door，replace or adjust the door
the door switch failure
switch.
Heater bad Replace the same model heater

5.CO2 Alarm CO2 cylinder low pressure Adjust valve to 0.06～0.08MPa

CO2 Increased the Regulator valve adjustment is too small,

concentration of slow adjusting it clockwise.
Regulator valve regulating valve opening is

CO2 Concentrations too big or too big. （ Counterclockwise

exceeded.
transfer Small regulator valve.）

6.CO2 Concentration Door not closed，(Fan not to

Open the door or adjust the door switch.
did not rise
switch)
Sensor broken Replace the same model sensor
Solenoid valve broken Replace solenoid valve
7.Temperature display
Uncorrected Amended in accordance with instructions.
not allowed
8.CO2 Concentration
Uncorrected Amended in accordance with instructions.
not allowed

﹕All the herein above should be done by a qualified person, turn off the power
to maintain！！
X.Storage and transportation
The incubator should be stored in excellent room whose relative humidity less

than 80%, non-corrosive gases . Transportation should be shock, moisture and has

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other necessary protective measures. Can not be inverted transport, handle with

care.

XI.After Sales
Our company has implement three warranty (including repair, replace or
return) of the incubator for one year (except heating element ). Under
warranty any damaged or not working which is because of the quality of the
product ,we responsible for free repair or replace components of the
product ;After one year， in line with the spirit of customer service,we will
make every effort to provide users convenience. Note:If you dismantle the
CO2 test kits inside，this will cancel the Warranty Service

Packing List

No. Descriptions Categories Quantities Notes

1 CO2 Cell Incubator machine 1
2 Humidity pan Fittings 1
3 Shelf Fittings 2 160L:3
4 Silicone tube for filling CO2 gas Fittings 1
5 Rubber tube for filling water Fittings 1
Wearing
6 Fuse tube（Φ5×20） 1
parts
7 Operation Manual Document 1
8 Product certificate Document 1
9 Warranty card Document 1
10 Packing List Document 1

The listed items are in compliance with the shipped goods

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