TARGET : PRE-MEDICAL 2023 ENTHUSIAST COURSE

TARGET : PRE-MEDICAL 2023

ENTHUSIAST COURSE
BIOLOGY
BIOTECHNOLOGY : PRINCIPLES AND PROCESSES
1. After completion of biosynthetic stage, the 5. In PCR, multiple copies of _____A_____ of
genetically engineered product is subjected interest is synthesised _____(B)_____ using
through a series of process known as : two sets of _____ (C) _____.
(1) Downward processing (1) A-RNA, B-in vivo, C-Primers
(2) Down grade processing (2) A-DNA. B-in vivo, C-Primers
(3) Upstream processing (3) A-Protein, B-in vivo, C-Primers
(4) Down stream processing (4) A-DNA, B-in vitro, C-Primers
2. The genetically engineered product has to be 6. The correct sequence in RDT is -
formulated with suitable ______ (A)____.
Such formulation has to undergo through (1) Isolation of DNA Fragmentation of DNA
_____ (B) ______ as in case of _____(C) Isolation of desired DNA fragment
______. Ligation of DNA fragment into vector
(1) A : Pesticides, B : Clinical Trial, C : Drugs Transferring recombinant DNA into host
(2) A : Drugs, B : Pesticides, C : Clinical Trial Expression
(3) A : Preservatives, B: Clinical Trial,C : Drugs (2) Isolation of desired DNA fragment
(4) A : Clinical Trial, B : Preservatives,C ; Drugs Fragmentation of DNA Ligation of DNA
3. How many among following are false: fragment into vector Isolation of DNA
A. Bioreactor can be a vessel in which raw Transferring recombinant DNA into host
materials are biologically converted into Expression
specific products.
(3) Isolation of DNA Ligation of DNA
B. Bioreactor assure not to provide optimum
fragment into vector Isolation of desired
growth condition.
DNA fragment Fragmentation of DNA
C. Most commonly used bioreactors are
Transferring recombinant DNA into host
without stirrer.
Expression
D. Air bubbles can be passed into bioreactor
according to requirement. (4) Ligation of DNA fragment into vector
(1) one (2) two Isolation of DNA Isolation of desired
(3) three (4) four DNA fragment Fragmentation of DNA
4. A. In almost all recombinant technologies the Transferring recombinant DNA into host
ultimate aim is to produce a desirable protein. Expression
B. Foreign gene gets expressed under suitable 7. Bacterial colonics that have a foreign DNA
condition. fragment inserted in to the plasmid will appear
C. If any protein encoding gene is expressed white, because-
in a heterologous host, it is called
(1) X-gal can be cleaved by P-galactosidase
recombinant protein
D. The cultures may be used for extracting (2) Gal gene shows insertional inactivation due
desired protein. to insertion of foreign DNA
How many among following are true. (3) Gal gene is active
(1) one (2) two (4) X-gal can be cleaved by permease
(3) three (4) four

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 TARGET : PRE-MEDICAL 2023 ENTHUSIAST COURSE
8. The cells can be multiplied in a ____(A)____ 15. Restriction enzyme ECoRI cuts DNA between
where in the used medium is drained from one bases G and A only when the sequence of DNA is:
side while fresh medium is added from the (1) GATATC (2) GAATTC
other to maintain the cells in their (3) GATTCC (4) GAACTT
physiologically most active ____(B)____. 16. Agrobacterium tumifaciens:
(1) A-Batch culture system, B-Lag phase A. Is a bacteria
(2) A-Continuous culture system, B-Log phase B. Acts as a natural genetic engineer
(3) A-Batch culture system, B-Log phase C. Has been used to transfer gene for RNA
(4) A-Continuous culture system, B-Lag phase interference in plants
9. Recipient cells are made ________ to take up mark the correct statements:-
DNA present in its surrounding. (1) A only (2) A and B only
(1) complete (2) component (3) B and C only (4) A, B and C
(3) complement (4) competent 17. Two enzymes responsible for restricting the
10. DNA polymerase used in PCR is isolated from: growth of bacteriophage in E. coli were
(1) Thermus aquaticus which is a bacteriaum isolated. One was methylase and other was
(2) Thermus aquaticus which is a fungi restriction endonuclease. What was the
(3) Thermus acidophillus which is a bacterium significance of methylase?
(4) Thermus acidophillus which is a fungi (1) Able to cut the DNA of bacteriophage at
11. Blue white selection is used: specific sites.
(1) To treat for the presence of plasmid in (2) Able to remove the methyl group and hence
bacteria prevent the action of restriction
(2) To reveal the identity of a cloned DNA endonuclease on host DNA
fragment (3) Protection of host DNA from the action of
(3) To express the product of a cloned gene restriction endonuclease by adding methyl
group.
(4) To test for the presence of a cloned insert
in a plasmid (4) Able to ligate the two cohesive ends of DNA
molecules.
12. DNA fragments generated by the restriction
endonucleases in a chemical reaction can be 18. Which of the following vectors is/are used for
separated by: cloning in eukaryotic organisms:
(1) Polymerase chain reaction A. Plasmids
(2) Gel electrophoresis B. Bacteriophage
(3) Restriction mapping C. Ti plasmid of Agrobacterium tumifaciens
(4) Centrifugation D. Disarmed retro viruses.
13. Which one of the following statement (1) A, B, C and D (2) A and B only
regarding DNA polymerase is true in PCR: (3) C and D only (4) D only
(1) It is used to ligate introduced DNA in 19. In E. coli cloning vector pBR 322 , rop codes for:-
recipient cell (1) Proteins involved in replication of plasmid
(2) It serves as a selectable marker (2) Proteins involved in antibiotic resistance
(3) It is isolated from a virus (3) Proteins involved in synthesis of antibiotic
(4) It remains active at high temperature (4) All of these
14. A single strand of nucleic acid tagged with a 20. PCR is used to :-
radio active molecule is called: (1) Detect HIV in suspected AIDS patients
(1) Vector (2) Detect mutations in genes in suspected
(2) Selectable marker cancer patients
(3) Plasmid (3) Identify many genetic disorders
(4) Probe (4) All of these

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 TARGET : PRE-MEDICAL 2023 ENTHUSIAST COURSE
21. Identify the correct match 25. The type of bond formed by DNA ligase
Column I Column II between adjacent nucleotides is
I. Cloning A. Making multiple (1) hydrogen bond
identical copies (2) phospho-diester bond
II. Biolistics B. Tumor (3) sulphide bond
III. Restriction C. Restriction enzyme (4) diesterbond
endonuclease 26. Which statement is wrong?
(1) Nucleic acid is fragmented by nucleases.
IV. T-DNA D. Gene gun
(2) Construction of recombinant DNA involves
(1) I - A, II - D, III - C, IV - B
cleaving DNA segments with endonuclease
(2) I - D, II - B, III - C, IV - A and rejoining with ligase
(3) I - C, II - B, III - A, IV - D (3) Genetic engineering is making artificial
(4) I - B, II - C, III - D, IV - A limbs and diagnostic instruments.
22. Which statement is true about bioreactor? (4) Ti plasmid transforms cells of plants.
A. Bioreactor provides the optimal conditions 27. For transformation with recombinant DNA, the
for obtaining the desired product. bacterial cells must first be made 'competent'
which means
B. Raw materials are biologically converted into
(1) Should increase their metabolic reactions
specific products
(2) Should decrease their metabolic reactions
C. A stirred tank reactor is horizontal in shape
(3) Increase efficiency with which DNA enters
D. Large volumes of cultures cannot the the bacterium
processed.
(4) Ability to divide fast
(1) A & B (2) B & C
28. During heat shock to the bacterium, the
(3) C & D (4) A, B, C & D temperature used for giving thermal shock is
23. In recombinant DNA methods, the term vector (1) 82°C (2) 100°C
refers to :-
(3) Liquid nitrogen (4) 42°C
(1) The enzyme that cuts DNA into restriction 29. Identify the true statements -
fragments
A. The first recombinant DNA was constructed
(2) The sticky end of a DNA fragment by using a piece of DNA from a plasmid
(3) A plasmid used to transfer DNA into living carrying antibiotic resistance gene in the
cell plasmid of bacterium Salmonella
typhimurium and transferred it to the E.
(4) A DNA probe used to identify a particular
coli.
gene.
B. Cohen and Boyer are known as father of
24. Elution is a method applied for:-
genetic engineering.
(1) Making the matrix during gel C. When cut by the same restriction enzyme,
electrophoresis the resultant DNA fragments have the same
(2) Staining the bands of DNA after kind of sticky ends and these can be joined
electrophoresis together using DNA ligases.
(3) Cutting the pieces of agarose gel and D. Endonucleases remove nucleotides from the
extraction of DNA from gel pieces. ends of the DNA whereas exonucleases
make cuts at specific positions within the
(4) Joining the specific DNA with the cloning
DNA.
vector.
(1) A & D (2) B & C
(3) A & C (4) C & D
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 TARGET : PRE-MEDICAL 2023 ENTHUSIAST COURSE
30. Identify the incorrect statement 35. In genetic engineering, recombinant DNA
(1) Presence of more than one recognition sites means:
within the vector will generate several
(1) DNA with a piece of RNA
fragments, which will complicate the gene
cloning. - (2) DNA with a piece of foreign DNA
(2) pBR322 vector was the first artificial ideal (3) DNA which takes part in recombination
vector constructed by Boliver and (4) DNA not associated with recombination
Rodrigues.
(3) Plasmid DNA is coated with histone proteins 36. Gene recombinant technology is used for:
and can act as genetic vector. (1) vectorless gene transfer into target cell
(4) None of these (2) direct transfer of DNA protein complex
31. Which is incorrect? (3) vector based gene transfer in to target cell
(1) EcoRI cuts the DNA between bases G and A.
(4) liposome base direct gene transfer into target
(2) Each restriction endonuclease recognizes
cell
a specific palindromic nucleotide
sequences in DNA. 37. Who invented recombinant DNA (r-DNA)
(3) When cut by same restriction enzyme, the technology?
resultant DNA fragments do not have the (1) James D. Watson
same kind of 'sticky-ends
(2) Har Gobind Khorana
(4) Making multiple identical copies of any
template DNA is called cloning. (3) Walter Sutton and Oswald Avery
32. If a DNA fragment is inserted in ampicillin (4) Stanley Cohen and Herbert Boyer
resistance gene in pBR322 then? 38. Which of the endonuclease is mostly used in
(1) both transformants and recombinants will genetic engineering?
grow in ampicillin containing medium
(1) Type I (2) Type II
(2) transformants will grow in ampicillin but
(3) Type III (4) (1) and (3)
recombinants will not
(3) non transformants will grow in ampicillin 39. Restriction enzyme was discovered by:
but transformants will not (1) Milstein and Kohler
(4) transformants will grow in tetracycline but (2) Temin and Baltimore
non-transformants will not
(3) Arber, Nathans and Smith
33. Some of the steps involved in Gene Cloning
are given below (4) Holley, Khorana and Nirenberg
(i) Insertion of isolated gene to the vector 40. In nature, the function of restriction enzymes
(ii) Introduction of recombinant vector to the host is to:
(iii) Isolation of desired gene (1) to cut plasmids
(iv) Expression of recombinant gene in host (2) destroy phage DNA
(v) Extraction of recombinant gene product
(3) splice DNA in a cell
The correct sequence of steps involved are
(4) destroy foreign DNA in animal cells
(1) iii, i, iv, ii, v (2) iii, i, ii, iv, v
(3) i, ii, iii, iv, v (4) ii, i, iii, iv, v 41. Molecular scissors, which cut DNA at specific site:
34. A technique of using very small metal particles (1) ligase
coated with desired gene in the gene transfer (2) cellulase
is called
(3) restriction endonuclease
(1) Electroporation (2) Microinjection
(4) polymerase
(3) Liposome (4) Biolistics

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 TARGET : PRE-MEDICAL 2023
42. Restriction enzymes:
(1) cut or join DNA fragments
(2) are required in vectorless direct gene
transfer
(3) are endonucleases which cleave DNA at
specific sites
(4) make DNA complementary to an existing
DNA or RNA
43. Which of the following is true concerning
restriction endonucleases? They :
(1) are carbohydrates
(2) destroy host cell DNA
(3) are transposable elements
(4) recognize and cut specific DNA sequences
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ENTHUSIAST COURSE
44. When a typical restriction enzyme cuts a DNA
molecule, the cuts are uneven, so that the DNA
fragments have single-stranded ends. These
ends are useful in recombinant DNA work
because:
(1) they serve as starting points for DNA
replication
(2) only single stranded DNA segments can
code for proteins
(3) they enable researchers to use the fragments
as molecular probes
(4) the fragments will bond to other fragments
with complementary ends.
45. A mixture containing DNA fragments, A, B,C
and D with molecular weights A + B = C, A >
B and D > C, was subjected to agarose gel
electrophoresis. The position of these fragments
from anode to cathode sides of gel should by:
(1) B, A, C, D (2) A, B, C, D
(3) C, B, A, D (4) B, A, D, C
5/5
 TARGET : PRE-MEDICAL 2023
ENTHUSIAST COURSE
BIOLOGY
BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

ANSWER KEY
Que. 1 2 3 4 5 6 7 8 9 10
Ans 4 3 2 4 4 1 2 2 4 1
Que. 11 12 13 14 15 16 17 18 19 20
Ans 4 2 4 4 2 4 3 3 1 4
Que. 21 22 23 24 25 26 27 28 29 30
Ans 1 1 3 3 2 3 3 4 3 3
Que. 31 32 33 34 35 36 37 38 39 40
Ans 3 4 2 4 2 3 4 2 3 2
Que. 41 42 43 44 45
Ans 3 3 4 4 1

TARGET : PRE-MEDICAL 2023

ENTHUSIAST COURSE
BIOLOGY
BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

ANSWER KEY
Que. 1 2 3 4 5 6 7 8 9 10
Ans 4 3 2 4 4 1 2 2 4 1
Que. 11 12 13 14 15 16 17 18 19 20
Ans 4 2 4 4 2 4 3 3 1 4
Que. 21 22 23 24 25 26 27 28 29 30
Ans 1 1 3 3 2 3 3 4 3 3
Que. 31 32 33 34 35 36 37 38 39 40
Ans 3 4 2 4 2 3 4 2 3 2
Que. 41 42 43 44 45
Ans 3 3 4 4 1