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LEOPATRA TECHNIQUE ™
PRF FACIAL AESTHETICS

CLEOPATRA TECHNIQUE ™
CLEOPATRA NACOPOULOS
BSc, DDS,MCD,MSc,MSC, MSc, PhD
@cleopatra_nacopoulos
SKIN REJUVENATION
• Derma Pen • Injections
Prof. Cleopatra Nacopoulos

• Derma roller
DIFFERENT TERMS OF SKIN NEEDLING
•Dermal needling

•Microneedling
•Meso-rolling
•CIT (Collagen Induction Therapy)
•PCT (Percutaneous Collagen Induction)
TECHNIQUES
Mesotherapy
WHAT IS SKIN NEEDLING ?

•Micro needles penetrate the epidermis and dermis layer generating
micro channels into the skin
• Pathway for products to penetrate more easily, boosting their
e ectiveness
• Promotes natural reproduction of collagen & elastin by stimulating
the body’s wound healing reaction to produce natural collagen
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MICRO-NEEDLING DEPTH



CONTRAINDICATIONS

•Papulopustular rosacea
•Acne vulgaris stage 3-4
•Herpes simplex
•Warts
•Scleroderma
•Bacteria/fungal infections
CONTRAINDICATIONS
•Open lessions
•Solar elastosis
•Skin cancer
•Hemophilia
•Pregnancy
PRECAUTIONS / CONSIDERATIONS

•Certain health conditions, medications, supplements
and lifestyle factors may affect the procedure
•Consultation Form prior to any clinical treatment
TREATMENT STEPS
History: e.g. recurrent herpes
infections (herpes prophylaxis -
aciclovir)
•Remove makeup
•Disinfection of the skin
•Anaesthesia according to how
deep we are going to insert the
needles inside the skin
Prof. Cleopatra
Micro needling
Nacopoulos
EASIEST WAY!!!
Prof. Cleopatra
PROCEDURE Nacopoulos
FOR MESOTHERAPY
VERY SAFE AND EASY AESTHETIC
PROCEDURE TO REJUVENATE YOUR
SKIN
•Improves vascularization
•Creates Collagenogenesis
•Improves Skin texture
•Improves Skin color
NEW PROTOCOL
i-PRF+
700rpm x 8 ‘
We treat the epidermis/ micro channels through

which products or iPRF+ are being absorb
into the skin
MICRONEEDLING WITH A DERMA ROLLER
• This treatment is more aggressive

• It needs more experienced participants
•The derma roller head includes 192-198 needles
• There are di erent shapes of heads to reach all facial areas

• There are are di erent lengths of needles for deeper penetration into the skin
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PROTOCOL
• One treatment every 15 days when the micro needling is
•
more super cial (0.25-1.5mm in depth)
Once every month when the treatment of micro needling is
deeper(more than 2mm in depth )
• 3-4 sessions totally
• Younger people may need less treatments compared to older
patients
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AFTER CARE
•Avoid the sun exposure

•Always wear sun protection cream SPF30%
•Hydration must be emphasized to patient
• 2.0mm roller treatment➔ maximum 1 week
• 0.5mm roller treatment➔ 2~4 days
IDEA
i-PRF+
Prof. Cleopatra
+ PRF liquid Nacopoulos
A-PRF liquid
FACIAL AESTHETICS

i-PRF+ A-PRF liquid
700rpm x 5 ‘ 1300rpm x 5’
PRF APPLICATIONS
• Facial Aesthetics

Keloids
•
• Acne Scars
• Hair regrowth
• Arthritis treatment
• Wound healing
• Complications due to fillers
Prof. Cleopatra
MATERIALS FOR Nacopoulos
VENIPUNCTURE
AESTHETICS
ONLY PURPLE 13ml TUBES

NEW PROTOCOL

i-PRF+ A-PRF liquid
700rpm x 8 ‘ 1300rpm x 8’
ANESTHESIA
• Topical anaesthetics EMLA 5%
• In ltration with local anaesthetic
• Nerve blocks is very rarely used
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INTRAORAL ANAESTHESIA

PRE-TREATMENT

Deep cleaning of the skin:
• Alcohol irritates the skin
• Better use Chlerexidine
• Best use CLEANING FOAM totally ORGANIC
Derma peel

• Always clean skin
• Remove dead cells
TECHNIQUES
Micro papular Mesotherapy

technique
TECHNIQUES

Smokers wrinkles
Linear threading or retro-tracking

TECHNIQUES

Marionette lines
Fan technique
Restoring and creating

volume
TECHNIQUES

Point by point or
Sandwich technique Cross-hatching serial puncture
technique technique
SKIN INJECTIONS

POINT BY POINT/
MICRO PAPULAR TECHNIQUE
MICRO PAPULAR
Cold mask after the
treatment
AUGMENTATION
•Restores lost volume
•Contouring
•Corrects asymmetries
•Treats acne scars

i-PRF+ A-PRF liquid
DERMIS
1300rpm x 8’
700rpm x 8 ‘
SUPERFICIAL
32g x 4mm
DEEP
30g x 12,8mm
27g x 12,8mm
30g x 4mm
CANNULA 22g
FACIAL AREAS
TIP AND TRICK
Prof. Cleopatra
•First step: injections
Nacopoulos
•Second step: Microneedling with
( Dermapen or derma roller)

•Consultation (photodocumentation)
•Pre-op instructions (avoid medications on

our restrictions list )
•Medical history
•Phlebotomy
•The entire process is over in as little as 45 minutes to just a few
hours
•Most patients can return to work later that day and even the next
day without issue
•Instructions on physical limitations, some medication
•Some dietary restrictions are given to each patient to help

expedite the healing process
PROTOCOL
• One treatment every 15 days when injections are more
•
super cial into dermis (up to 4mm in depth)
Once every month when the injections are deeper into the
skin(more than 13mm in depth ), that means more
aggressive treatment
• 3-4 sessions totally
• Younger people may need less treatments
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POST TREATMENT
Prof. Cleopatra NacopoulosICE LowLevelLedTherapy

MASK
•Ice pads on the treated area

•Avoid sun exposure/ heavy exercise
•Sun protection cream/SPF 30%
•Arnica ointment topically on the
bruises
•Bepanthol cream 3-4 times a day for
at least two weeks
MAINTENANCE
Prof. Cleopatra
•Condition of the skin Nacopoulos
•Age of the patient
•Life style
•Physical condition
Usually we repeat the treatment once or twice a year


PRF & HYALURONIC ACID
•HA is a natural polysaccharide occurring in our skin
•Holds up 1.000 times its own weight in moisture
•Moisturizes, plumps, and gives a rmer-looking skin
•Youthful appearance with a luminous glow
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Prof. COMPLICATIONS
CleopatraDUE Nacopoulos
TO FILLERS
Application of liquid platelet-rich brin (PRF) for treating hyaluronic acid-
related complications: Acase report with 2 years of fellow up
International journal of Growth Factors and Stem Cells in Dentistry

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HAIR TREATMENT
PROTOCOL
•One treatment every

month
•4-6 treatments
•Can be combined with
cocktails of vitamins and
minerals
•Minoxidil (ROGAINE) or
Nanoxidil
ARTHRITIS TREATMENT
PROTOCOL
Prof.
• Cleopatra
One treatment
4-6 treatments
•
every 15 days for Nacopoulos
the hands
• Light massage after treatment

• One treatment every month for the knee
• 4-6 treatments according to the patient’s satisfunction
NUTRITION
«A personalized nutrition plan based on genetic
profile improves outcomes of facial
regeneration with Platelet-Rich Fibrin liquid
matrices»

Plastic and Aesthetic Research
Nacopoulos C.et all 2021
• We saw the importance of personalized nutrition, as it was

determined by the patient’s DNA, to the improvement of
the outcome of CLEOPATRA TECHNIQUE ™
• A proper nutrition plan may effect additionally any
aesthetic procedure
•Patients who followed the personalized nutrition for 3 months
showed greater improvement to the appearance of their face
•The evaluation was done by Facial Wrinkles Assessment
Scale
•The nutrition plan was given to the patient by a nutritionist
who was expert in nutrigenetics
•The information was given by GENOSOPHY test
•The test studies the polimophisms of 120 genes which
show the nutrition react in combination with

macronutrients such as proteins, carbohydrates and fats
•The need of supplements
•Antioxidants
•The need of a personalized diet plan

Prof. CleopatraPLATELET
Nacopoulos
RICH FIBRIN
IN REGENERATIVE DENTISTRY
Chapter 13

Thank y f y r
a enti
tt
o
ou
or
ou

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LEOPATRA TECHNIQUE ™

PRF FACIAL AESTHETICS

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

We treat the epidermis/ micro channels through

• This treatment is more aggressive

Prof. Cleopatra Nacopoulos

• There are di erent shapes of heads to reach all facial areas

•Avoid the sun exposure

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

ONLY PURPLE 13ml TUBES

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Micro papular Mesotherapy

Prof. Cleopatra Nacopoulos

Linear threading or retro-tracking

Prof. Cleopatra Nacopoulos

Restoring and creating

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

•Treats acne scars

Prof. Cleopatra Nacopoulos

•Pre-op instructions (avoid medications on

•Instructions on physical limitations, some medication

•Some dietary restrictions are given to each patient to help

Prof. Cleopatra NacopoulosICE LowLevelLedTherapy

•Ice pads on the treated area

Usually we repeat the treatment once or twice a year

Prof. Cleopatra Nacopoulos

International journal of Growth Factors and Stem Cells in Dentistry

Prof. Cleopatra Nacopoulos

•One treatment every

• Light massage after treatment

Prof. Cleopatra Nacopoulos

• We saw the importance of personalized nutrition, as it was

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

Prof. Cleopatra Nacopoulos

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