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SAFE 126: Class Lab Safety Tutorial

Date: March 2024
Time: ……..
SECTION A (20 Marks): Encircle the best answer.

1. Class II Biological Safety Cabinets and nose should be completely covered by

provide protection to which of the PPE.
following a. True
a. Personnel only. b. False
b. Product only. 6. The most reliable means of sterilisation is:
c. Personnel and environment. a. Ethylene oxide
d. Personnel, product and environment b. Steam
2. When it comes to first aid proceed as c. Dry heat
follows; d. Plasma
a. Inform, protect and assist 7. Laboratory safety practices include:
b. Protect, assist and inform a. Chemical management
c. Assist, protect and inform b.Testing how harmful an organism is
d. Protect, inform and assist c. Keeping lab safety documents in a safe
3. Decontamination results in an item that is and locked drawer
safe for patient reuse. d.All of the above
a. True 8. A chemical label contains:
b. False. a. statement of hazard
4. Disinfection: b. hazard class
a. Is used for items that will contact c. safety precautions
intact skin d. All of the above
b. Involves chemical agents 9. Hand washing is a crucial component of
c. Reduces the numbers of both infection control and chemical
microorganisms hazard control
d. All of the above a. True
5. When working with risk group four agents b. False
the mucous membranes of eyes, mouth

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c. Chemical safety is different from a. Impermeable lab coat with eye/face

biological safety hence their safety is protection and appropriate disposable
also different. gloves
10. If the eyes are affected take the patient to b. Respirators with HEPA filter
a medical centre with the LABEL or c. Gloves with rubberized sleeves
MSDS of the product. d. Safety glasses for individuals not
a. True wearing contact lenses
b. false 15. A fire caused by a flammable liquid
11. After any work with highly toxic or should be extinguished using which type
carcinogenic chemicals, the face should of extinguisher?
also be washed a. Class B
a. True b. Halogen
b. False c. Pressurized water
12. Which of the following standards requires d. Class C
that SDSs are accessible to all employees 16. Which of the following is the proper
who come in contact with a hazardous means of disposal for the type of waste?
compound? a. Microbiologic waste by steam
a. Hazard Communication Standard sterilization
b. Bloodborne Pathogen Standard b. Xylene into the sewer system
c. CDC Regulations c. Mercury by burial
d. Personal Protection Equipment d. Radioactive waste by incineration
Standard 17. What are the major contributing factors to
13. Chemicals should be stored repetitive strain injuries?
a. According to their chemical a. Position/posture, applied force, and
properties and classification frequency of repetition
b. Alphabetically, for easy accessibility b. Inattention on the part of the
c. Inside a safety cabinet with proper laboratorian
ventilation c. Temperature and vibration
d. Inside a fume hood, if toxic vapors can d. Fatigue, clumsiness, and lack of
be released when opened coordination
14. Proper PPE in the chemistry laboratory 18. Which of the following are examples of
for routine testing includes nonionizing radiation?
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a. Ultraviolet light and microwaves 21. The purpose of Bloodborne Pathogens

b. Gamma rays and x-rays Standard is
c. Alpha and beta radiation a. To protect health-care workers from
d. Neutron radiation occupational ingestion of bloodborne
19. One liter of 4 N sodium hydroxide (strong pathogens
base) in a glass 1 L beaker accidentally b. To protect health-care workers from
fell and spilled on the laboratory floor. occupational exposure to bloodborne
The first step is to: pathogens
a. Call emergency number c. To provide health-care workers
b. Alert and evacuate those in the treatment to bloodborne pathogens
immediate area out of harm's way d. To protect health-care workers from
c. Throw some kitty litter on the spill occupational testing on bloodborne
d. Squirt water on the spill to dilute the pathogens
chemical 22. The purpose of Hazard Communication
e. Neutralize with absorbing materials in Standard is
a nearby spill kit a. Is to provide a written hazard
20. Of the following, which is NOT communication plan
reportable to the Department of safety? b. To provide inventory of hazardous
a. A laboratorian with a persistent cough chemicals on site & PPE
that is only triggered at work c. To inform employees about chemical
b. A laboratorian that experienced a hazards in workplace & protective
chemical burn measures
c. A laboratorian that tripped in the lab d. To provide a requirement for
and hit her head on the lab bench accreditation and registration
rendering her unconscious 23. _________ is an example of corrosive
d. A laboratorian that was stuck by a chemical
contaminated needle after performing a. NaOH
phlebotomy on a patient b. Alcohol
e. A laboratorian that forgot to wear his c. H2O
lab coat and gloves while diluting d. NaCl
patient serum
f. All situations are worth reporting
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24. On National Fire Protection Association avoided and safer sharps devices used
(NFPA) Hazmat Diamond, instability is where available
indicated by a. True
a. Blue diamond b. False
b. Yellow diamond
c. White diamond 29. If the NSI / sharps injury is ‘high risk’
d. Black diamond (deep injury; visible blood on the device
25. Which of the following is associated with causing injury) and the source is HIV
storage of Water-reactive chemicals? positive or at high risk of HIV, PEP will
a. away from water. be prescribed.
b. dry place a. True
c. Cool place b. False
d. All of the above 30. Blood Borne Virus (BBV): a virus which
26. Which of the following is true for is carried in the blood of infected
electrical fire? individual and which cannot be
a. You can use pressurized water (A) transmitted to another person exposed to
b. CO2 leaves no residue, good choice the individual’s blood.
for computers and analysers. a. True
c. It is class B fire b. False
d. All of the above 31. Lab work areas should be kept untidy at
27. The following are the common routes of all times
laboratory exposure. a. True
b. False
a. Inhalation and Ingestion
32. The following are examples of personal
b. Injection and Skin absorption protective equipment except

c. Both A and B a. Gloves

d. None of the above b. Gowns

28. In relation to sharps injuries, wherever c. Shields

possible the use of Sharps should be
d. Safety hood

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33. When working in the lab always be alert 36. If a chemical splashed in your eye(s) or on
a. True your skin, immediately do not flush with
b. False running water for at least 20 minutes.
34. Dress properly during a laboratory a. True
activity. Long hair, dangling jewellery b. False
(like necklaces), and loose or baggy 37. Never return unused chemicals to their
clothing are a hazard in the laboratory. original container
a. True a. True
b. False b. False
35. Without effective segregation system, the 38. Check glass and plastic centrifuge tubes
complete waste stream must be for stress lines, hairline cracks and
considered as hazardous. chipped rims before use.
a. True
a. True
b. False
b. False

39. Mask of cotton wool, gauze, or paper
masks are ineffective. Paper mask with
synthetic material for filtration are an
effective barrier against microorganism
a. True
b. False
40. Laboratory housekeeping: safety of lab
workspace and the ventilation provided is
compromised when excessive chemicals
and equipment are kept in the space.
a. True
b. False

SECTION B: Answer all questions and be specific to the point

1. Define the following in relation to laboratory safety (4 Marks)

a. Standard precautionary measures

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b. Decontamination

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2. Differentiate the following in relation to laboratory safety (6 Marks)

a. Ergonomic hazard from mechanical hazard

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b. Biosafety cabinet class II from Biosafety cabinet class III

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c. Particulate respirators from gas masks

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3. List five design features of biosafety level 4 maximum containment laboratory? (5 Marks)
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4. Explain three Ps of first aid aims (6 Marks)
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5. Explain how spores are killed using steam under pressure (2 Marks)
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6. Mention three chemical agents that can be used for disinfection in the laboratory? (3 Marks)
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7. Based on question 6, choose any two mentioned chemicals and explain their mechanism of
action as disinfectants (4 Marks)
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8. Using a pencil/pen, roughly draw, two laboratory symbols depicting glassware hazard and
carcinogenic hazard (4 Marks)
9. Outline six steps involved in proper waste management (6 Marks)
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10. Describe the triple packaging protocol in relation to transportation of a blood sample (5 Marks)
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11. Write five methods of sterilization techniques (5 Marks)
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12. Assume that you are in an infectious disease diagnostic laboratory and your co-worker has been
pricked by a needle, which was contaminated by blood. In well summarised steps explain how
you can do the first aid procedure and help the colleague undergo post exposure prophylaxis.
(10 Marks)
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The End

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