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    (Week - 13b) Bioprocess Engineering

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    robot2410
    1. The document discusses various methods for producing biochemicals such as ethanol, succinic acid, lactic acid, and Coenzyme Q10 through fermentation. Key microorganisms discussed include Saccharomyces cerevisiae, Zymomonas mobilis, and Corynebacterium glutamicum. 2. Methods to improve xylose fermentation in S. cerevisiae through introduction of xylose pathway enzymes from other organisms are described. Production of erythritol through fermentation by yeasts such as Candida is also covered. 3. Production of chemicals such as sorbitol, gluconic acid, and isosorbide through fermentation of glucose or other sugars using

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    (Week - 13b) Bioprocess Engineering

    Uploaded by

    robot2410
    7 views49 pages
    1. The document discusses various methods for producing biochemicals such as ethanol, succinic acid, lactic acid, and Coenzyme Q10 through fermentation. Key microorganisms discussed include Saccharomyces cerevisiae, Zymomonas mobilis, and Corynebacterium glutamicum. 2. Methods to improve xylose fermentation in S. cerevisiae through introduction of xylose pathway enzymes from other organisms are described. Production of erythritol through fermentation by yeasts such as Candida is also covered. 3. Production of chemicals such as sorbitol, gluconic acid, and isosorbide through fermentation of glucose or other sugars using

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    [Week_13b] Bioprocess Engineering

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    1. The document discusses various methods for producing biochemicals such as ethanol, succinic acid, lactic acid, and Coenzyme Q10 through fermentation. Key microorganisms discussed include Saccharomyces cerevisiae, Zymomonas mobilis, and Corynebacterium glutamicum. 2. Methods to improve xylose fermentation in S. cerevisiae through introduction of xylose pathway enzymes from other organisms are described. Production of erythritol through fermentation by yeasts such as Candida is also covered. 3. Production of chemicals such as sorbitol, gluconic acid, and isosorbide through fermentation of glucose or other sugars using

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    © All Rights Reserved
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    7 views49 pages

    (Week - 13b) Bioprocess Engineering

    Uploaded by

    robot2410
    1. The document discusses various methods for producing biochemicals such as ethanol, succinic acid, lactic acid, and Coenzyme Q10 through fermentation. Key microorganisms discussed include Saccharomyces cerevisiae, Zymomonas mobilis, and Corynebacterium glutamicum. 2. Methods to improve xylose fermentation in S. cerevisiae through introduction of xylose pathway enzymes from other organisms are described. Production of erythritol through fermentation by yeasts such as Candida is also covered. 3. Production of chemicals such as sorbitol, gluconic acid, and isosorbide through fermentation of glucose or other sugars using

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    of 49

    Prof.

    Jun-Seob Kim
    •
    • (biocatalyst)
    •
    •
    •

    • ( 14-1).
    • 10 20
    • 6 NAD+ ( )
    • alcohol dehydrogenase NADH NAD+
    •

    • (3-PG, 2-PG, PEP) ,


    • 5% 17 , 17 , 17 NAD +
    • 3 NADH 3
    cellulosic biomass

    • :

    • , ,
    •
    •
    • pyruvate decarboxylase alcohol dehydrogenase
    • 80g/L 39g/L 0.72g/L·h

    • , ,
    •
    • (>60g/L) .
    •
    Zymomonas mobilis
    • .
    • GRAS
    • (~120g/L)
    • -
    ATP

    •
    .
    .
    Saccharomyces cerevisiae
    •
    •
    •
    • xylose
    xylulose

    xylose isomerase(XI) /
    XI
    XI
    P. stipitis xylose reductase, xylitol dehydrogenase,
    xylulokinase
    • , , ,
    •

    •
    •
    •
    • 60%
    •

    •
    •
    NADH

    •
    S. cerevisiae

    pH7
    •
    GPD1/2
    NADH PDC/ADH1/NDE/GUT2
    NAD+ FDH1/ALD3
    Triose phosphate isomerase 90%
    •

    , , ,

    ,
    •
    • , , ,
    •
    • ,

    •
    , ,
    •
    • ,
    •
    •

    • ,
    •
    •
    •
    •
    •

    • , , ,
    • (C.
    tropicalis C. guilliermondii ) S. cerevisiae
    •
    , 3.9g
    xylitol/L·h( ) 4.94g xylitol/L·h( )
    • S. cerevisiae P.
    stipitis XR XYL1

    • / (g/g) 70~80%
    100%
    • 200g/L
    ( S. cerevisiae )
    •

    Debamyoryces hansenii, Acetobacter suboxydans C. guilliermondii

    Bacillus subtilis S. cerevisiae


    • , ,
    •
    • 60%
    , ,

    • C ,
    •

    • , isosorbide, polyethylene glycol,


    ethylene glycol, glycerol, lactic acid
    • isosorbide
    •
    Z. mobilis
    glucono- -lactone ,
    NADP+ glucose-fructose oxidoreductase(GFOR, EC 1.1.1.99)

    • Z. mobilis , 11%
    • +

    290 g sorbitol/L, 283 g gluconic acid/L


    • cetyltrimethylammonium bromideCTAB :
    98~99%, 1.8 g/L·h, 2.1 g/L·h
    •
    • invertase

    • 200 g/L,
    5.1 g sorbitol/L·h, 92%
    • 60~70%
    • ,
    •
    •
    • fructose-6-phosphate phosphoketolase
    erythrose-4-phosphate erythrose-4-phosphate
    erythrose-4-phosphate dehydrogenase erythritol-4-phosphate
    phosphatase
    • glucose-6-phosphate pentose phosphate pathway
    erythrose-4-phosphate erythrose-4-phosphate kinase
    ,

    •
    • C. magnoliae, T. corallina, Ustilaginomycetes
    • 40%
    • C. magnolia 50L
    200 g erythritol/L
    1.2 g erythritol/L·h
    0.43 g erythritol/g sugar
    •
    • Agaricus bisporus
    • 0
    • 25% , 75%
    • ( )
    (70%)
    50% 50%

    , , ,
    •
    50~52%
    50~52%
    (Leuconostoc Lactobacilli group III, obligatory
    heterofermentative)
    •
    • lactic acid, succinic acid

    • polylactide
    •
    •
    • : homolactic fermentation

    • ( ) : heterolactic fermentation

    • : 1 2 ,2

    • : aldolase phosphoketolase .
    , ,
    • :
    •

    • L D
    • L
    L
    ( )
    • succinic acid 4 dicarboxylic acid
    • Corynebacterium glutamicum

    • Mucor, Fusarium, Aspergillus , , Aerobacter


    aerogen, Propionibacterium, Mannheimia succiniciproducens,
    Anaerobiospirillum succiniciproducens
    ( )
    • , ,
    • 1,4-butanediol, gamma-butyrolactone,
    tetrahydrofuran,
    • M. succiniciproducens, A. succiniciproducens

    ,
    ( )
    •

    , ,

    •
    Yarrowia lipolytica acetyl-CoA hydrolase
    , , TCA

    , (>100g/L)
    Q10

    Q10
    • ATP

    :
    :
    •
    , stain
    Q10

    Q10
    • Q10
    Agrobacterium, Rhadobacter
    (decaprenyl diphosphate synthase 1-deoxy-D-xylulose 5-
    phosphate synthase) , Q10
    • A. tumefaciens , 626.5
    mg/L, 9.25 mg/g cell
    • R. sphaeroides 8.7 mg/g Q10
    Q10

    Q10
    • Q10
    •
    • Q8
    • decaprenyl diphosphate synthase , 1-deoxy-D-xylulose 5-
    phosphate synthase 99.4 mg/L·h
    Q10
    •
    • , ,
    •
    :
    :
    , :
    , , :
    , :
    •

    : S-aminoethylcysteine
    • ( B2) ,
    •
    •
    • R. palustris cyclohexane carboxylate

    • B5 : A ,
    Q&A

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