Pablo Borbon Campus

College of Nursing and Allied Health Sciences

Name: _______________________________________ Date: ___________

Section: ________________________ Group No. ______

Biochemistry
Biosynthetic Pathways
Laboratory Sheet 5
Biosynthesis Amino Acid
“Amino Acid Biosynthesis in Living Organism”
I. Objectives
a.Check accuracy of amino acid metabolic reactions
b.Verify statements about amino acid.
c.Assess knowledge of amino acid pathways.

II. Materials
a.Ballpen
b.Printed copy of the worksheet.

III. Methodology
a.Prepare the materials
b.Ensure that the answer sheet is complete and accurate.
c.Analyze the results to identify areas of strength and weakness in the participants' knowledge.

A. ANALYSIS
1. What is biosynthesis of amino acids?

2. What makes the biosynthesis of amino acids distinct from the biosynthesis of lipids or carbohydrates?

3. Amino acids are classified into two groups: _______________ and _________________
4. Give the 9 essential amino acids

5. Give the 11 essential amino acids

Rizal Avenue Extension, Batangas City, Philippines +63 43779 - 8400 loc. 1127

www.batstate-u.edu.ph conahs.pb@g.batstate-u.edu.ph
 Pablo Borbon Campus

College of Nursing and Allied Health Sciences

B. TRUE OF FALSE
1. Triose phosphate isomerase converts dihydroxyacetone phosphate to glyceraldehyde 3-phosphate
through an intermediate with a carbon-carbon double bond. _____________
2. Carbon dioxide is added to pyruvate to make oxaloacetate via activated carbamoyl phosphate, using
the cofactor, biotin. ___________
3. The urea cycle is limited to the tissues of the kidney, where urea is removed from the blood.
___________
4. Arginine, ornithine, and citrulline are all amino acids used as intermediates in the Urea Cycle.
___________ 5. Ubiquinone, cytochrome c, NAD+, FAD, iron-sulfur clusters, and O2 accept
electrons during electron transfer reactions along the respiratory chain. ___________
6. The proton motive force is only comprised of the electrical potential energy imparted when protons
build up charge on one side of the membrane. ____________
7. The adenine nucleotide translocase exchanges an ATP in the matrix for an ADP in the
intermembrane space, requiring the expenditure of some energy, i.e., one ATP per every four
exchanges. ___________

C. MULTIPLE CHOICE
1.) 3-phosphoglycerate is not the metabolic precursor for ____________
a. Serine c. Cysteine
b. Glycine d. Arginine
2.) Pyruvate is the precursor for ____________
a. Alanine c. Serine
b. Glutamate d. Proline
3.) The cyclized derivative of glutamate is ____________
a. Proline c. Glutamine
b. Arginine d. Serine
4.) Precursor of glycine is ____________
a. Proline c. Serine
b. Glutamine d. Glutamate
5.) Which of the following is a non-essential amino acid?
a. Methionine c. Lysine
b. Threonine d. Cysteine
6.) Which of the following gives rise to methionine, threonine, and lysine?
a. Pyruvate c. Aspartate
b. Glutamate d. Serine
7.) Which of the following gives rise to valine and isoleucine?

Rizal Avenue Extension, Batangas City, Philippines +63 43779 - 8400 loc. 1127

www.batstate-u.edu.ph conahs.pb@g.batstate-u.edu.ph
 Pablo Borbon Campus

College of Nursing and Allied Health Sciences

a. Pyruvate c. Aspartate
b. Glutamate d. Serine
8.) Which of the following is not an aromatic amino acid?
a. Phenylalanine c. Tryptophan
b. Tyrosine d. Leucine
9.) Which of the following can be formed by hydroxylation of phenylalanine?
a. Serine c. Tryptophan
b. Tyrosine d. Leucine
10.) Phosphoribosyl pyrophosphate is a precursor of tryptophan and ____________
a. Tyrosine c. Phenylalanine
b. Histidine d. Isoleucin

D. Guide Questions

1.What is the initial reaction when Methionine interacts with ATP?

Rizal Avenue Extension, Batangas City, Philippines +63 43779 - 8400 loc. 1127

www.batstate-u.edu.ph conahs.pb@g.batstate-u.edu.ph
 Pablo Borbon Campus

College of Nursing and Allied Health Sciences

2. How is the methyl group activated, and where can it be transferred?

3. What is the product of the reaction described in 2?

4. How is S-adenosylhomocysteine formed from the previous reaction product?

5. What are the two fates of homocysteine?

Rizal Avenue Extension, Batangas City, Philippines +63 43779 - 8400 loc. 1127

www.batstate-u.edu.ph conahs.pb@g.batstate-u.edu.ph
 Pablo Borbon Campus

IV. References:
https://mcb.berkeley.edu/labs/krantz/mcb102/MCB102-SPRING2008-EXAM-KEY_v3.pdf
https://www.sanfoundry.com/biochemistry-questions-answers-biosynthesis-amino-acids

https://www.medschool.lsuhsc.edu/biochemistry/Courses/Biochemistry201/Desai/Amino%20Acid%20M
etabol ism%20I%2010-14-08.pdf
https://onlinelibrary.wiley.com/doi/abs/10.1002/9780470015902.a0000628.pub2#:~:text=The%20biosynt
hesis %20of%20amino%20acids,includes%20the%20use%20of%20nitrogen.
https://study.com/learn/lesson/essential-nonessential-amino-acids-
differences.html#:~:text=There%20are%20nine%20essential%20amino,proline%2C%20serine%2C%20
and%20ty rosine.

Rizal Avenue Extension, Batangas City, Philippines +63 43779 - 8400 loc. 1127

www.batstate-u.edu.ph conahs.pb@g.batstate-u.edu.ph