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Mitochondria (pg 1-3)

1. Phospholipid molecules are components of cell membranes that surround organelles such as mitochondria
and chloroplasts.

Outline the role of membranes within the cell.

[2]

2. Fig. 19.2 is a transverse section of a sperm cell. The mitochondria of sperm cells form a spiral around the
central flagellum.

Fig. 19.2

i. Identify the structures labelled with the following letters:

U
W
Z

[3]

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ATP, FADH2 and hexose 1,6-bisphosphate are three organic products of respiration in sperm cells.

Table 19 shows how the production of ATP, FADH2 and hexose 1,6-bisphosphate in sperm cells is
affected by three different substances.

Table 19

ii. What can be concluded about the difference between the effects of cyanide and fluoride
on respiration in sperm?

[1]

3. The endosymbiosis theory suggests that mitochondria may have evolved from bacteria that were taken
inside other cells.

These cells then evolved into eukaryotes.

i. Give two structural features of mitochondria that support this theory.

[2]

ii. Explain why early eukaryotes were able to grow more quickly than cells that did not possess
mitochondria.

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[3]

4. Brown fat is a type of tissue.

Brown fat has a higher need for oxygen because fat cells in this tissue carry out aerobic respiration at a higher
rate than fat cells in other tissues.

Suggest which organelle is present in higher numbers in brown fat cells than in other fat cells.
[1]

5(a). The image below is a photomicrograph of a mitochondrion.

i. State the type of microscope used to produce this image.

[1]

ii. Identify the structures labelled M and N in the photomicrograph.

M .....................................................................................

N .....................................................................................

[2]

(b). Describe two ways in which the structure of mitochondrial membranes is related to the function of a
mitochondrion.

[2]

END OF QUESTION PAPER

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Mark scheme

Question Answer/Indicative content Marks Guidance

CREDIT ‘controls what enters and


leaves organelles’ or ‘to keep
chemical reactions separate from
other reactions within the cell’

CREDIT reference to specific


organelle plus contents being
separates organelle contents from
separated e.g. lysosome AND
other parts of the cell
digestive enzymes
OR
OR
separates the cell into compartments
mitochondria AND respiratory
/ AW;
enzymes
OR
1 2 max sarcoplasmic reticulum AND calcium
reference to a named example;
ions
OR
vesicles AND proteins

e.g. holds ETC components


AVP;

Examiner's Comments

In this part, too many candidates


assumed the question referred to the
cell surface membrane and it was
rare to see any reference to
compartmentalisation.

Total 2

2 i U matrix ✔ 3

W crista(e) / inner (mitochondrial) IGNORE ETC / ATP synthase /


membrane ✔ cytochromes

Z inter-membrane space ✔
ALLOW inter-membranal space

Examiner’s Comments
Q19(c)(i) was generally well-answered
although some candidates failed to
interpret the diagram correctly and
gave totally irrelevant structures as
their answers. The most common
mistake was failing to identify the

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inter-membrane space or referring to
it as the inner-membrane space.

BOTH statements required for one


mark
IGNORE ‘affects’ throughout
ALLOW link reaction / Krebs cycle /
ETC / oxidative phosphorylation
instead of aerobic respiration
ALLOW cyanide allows, glycolysis /
anaerobic respiration
cyanide, prevents / AW, aerobic
respiration

ALLOW prevents, all respiration / both


ii 1
stages of respiration
IGNORE lactate fermentation
AND
Examiner’s CommentsQ19(c)(ii) saw
fluoride, prevents / AW, anaerobic some strong responses with
respiration (which also prevents candidates using data to support their
aerobic respiration) ✔ answer even though it was not
required. Weaker candidates gave
vague answers about how fluoride
and cyanide ’affected‘ respiration or
repeated the information in the table
without attempting a conclusion.

Total 4

length / size , similar to that of a


If more than two responses given:
bacterium ✓
mark first response on each prompt
contain (circular) DNA ✓ max 2
line.
3 i contain (70S / small / 20nm) (AO3.2)
If responses on first prompt line and
ribosomes ✓ (AO2.1)
nothing on second line then mark first
(may) have plasmids ✓
two on first prompt line
have double membrane ✓

cells with mitochondria / early


Assume for cells with mitochondria
eukaryotes
Only need to mention ATP once
ALLOW ORA for cells without
1 would be able to respire aerobically
mitochondria for MPs 1, 2, 4

3
ii 2 (this) produces more ATP ✓
(AO2.1) ALLOW releases more energy
3 ATP needed for , active transport /
DO NOT ALLOW ‘produces’ energy
cell division / protein synthesis / DNA
IGNORE growth
replication ✓
ALLOW more ATP so can meet higher
4 more ATP allows faster metabolic ,
metabolic demand
processes / reactions ✓

Total 5

4 mitochondria / mitochondrion 1 (AO2.5)

Total 1

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1 ALLOW TEM, ‘microscopy’ for
5 a i transmission electron (microscope) ✓
(AO2.1) ‘microscope’

M = matrix ✓ 2
ii ALLOW inner membrane for N
N = crista(e) ✓ (AO1.1)

contain / location of , (named)


electron carriers / ETC / ATP
synth(et)ase / proton pumps ✓

(provide , site / location / surface ) for


, chemiosmosis / ATP synthesis /
oxidative phosphorylation ✓ max 2
b Mark as continuous prose
(AO1.1)
allow , formation / maintenance , of ,
H+ / proton / hydrogen ion , gradient ✓

outer membrane is highly permeable


to allow movement of (named)
molecules ✓

Total 5

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