Annexure ‘AAB-CD-01’

Course Title: Molecular and Clinical Diagnostics L T P/ SW TOTAL CREDIT

S UNITS
Course Code: to be decided later
Credit Units: 6 4 0 4 0 6
Level: UG

# Course Title:
1 Course Objectives:

: 6-credit-hour course designed to provide students to acquire knowledge on the

organization of the clinical diagnostic laboratory and of principles and
methodologies associated to the most advanced diagnostic techniques.

2 Prerequisites: Basic understanding of cell biology and molecular Biology

3 ●

4 Module I : Introduction to Cellular pathology 20%

Weightage
Cellular Complexity: Cell components, Cell Differentiation, Cellular communication Normal Vs diseased cells,
cell adaptations, cell injury, inflammation, necrosis. Principle, types and application of different
microscopes.
5 Module II Molecular Diagnostics 20%
Weightage
PCR Principle, procedure, types and applications. RFLP maps, RAPD, DNA sequencing methods – principle,
types, automated process, overview of Next-generation sequencing (NGS)

6 Module III Biomarkers in Disease Diagnostics 20%

Weightage
 Disease Markers, Role of markers in Disease diagnosis. approaches and methods in
the identification of disease markers, predictive value, diagnostic value, emerging
blood markers for sepsis, tumor & cancer markers.
7 Module IV Cytogenetic analysis 20 %
Weightage
Human disorders and Cytogenetic analysis,Chromosome organization,. X-chromosome
dosage compensation and inactivation mechanism. Sex determination and Y
chromosome, Uniparental disomy, Genomic Imprinting and disorders. Principle of FISH,
CGH, Flow cytometry techniques and clinical diagnostics.

8 Module V : Immunodiagnostic techniques 20 %

Weightage
Immunodiagnostic techniques: Introduction, Radioactive isotopes, DNA reporters,
fluorogenic reporters, electro-chemiluminescent tags & label free immunoassays.
Immunocytochemistry, immunohistochemistry
Labs:
 Sample preparation - Specimen collection, handling, preparation, processing, containment, barcoding,
and tracking.
 Microscopy – light, fluorescent and confocal microscopy
 Animal Cell Media Preparation.
 Culture of Human Lymphocytes
 Cell Viability Assays
 Isolation of genomic DNA from peripheral blood and agarose gel electrophoresis.
 DNA - qualitative and quantitative analysis by spectrophotometry.
 RNA isolation (from rat liver).
 Polymerase chain reaction (PCR)
 Protein Estimation Techniques.
 SDS-PAGE and Western blotting
 Principle of Flowcytometry , ELISA and RIA
 Hem agglutination
9 Pedagogy for Course Delivery:
Theory:
Lectures: 57 Sessions
Class Test: 2 Sessions
Presentation/ Quiz :1
Total: 60 Sessions
Lab/ Practical:
Practical: 58 Sessions
 Class Test: 2 Sessions
Total: 60 Sessions

Course Learning Outcomes:

 To know the limits and applicability of the different methods used in advanced clinical
diagnostics.

 To understand the potential of different techniques and potential applications in clinical and
preclinical / experimental settings.

 To apply the use of different biomarkers and diagnostic criteria related to different human
diseases
● Demonstrate an understanding of how knowledge of pathological diagnosis
can be utilized in the investigation, and prevention of disease
● To analyse basic concept of diagnostics in clinical settings

10 Assessment/ Examination Scheme:

Theory L/T (%) Lab/Practical/Studio (%) End Term Examination

67 33 100%

Theory Assessment (L&T):

Continuous Assessment/Internal Assessment
(50%) End Term
Examination
Components Class presentation Home Quiz Attendance
(Drop down) test Assignme (50%)
nt

Weightage (%) 20 10 10 5 5 50

Theory Assessment (L&T):

 Continuous Assessment/Internal Assessment End Term
(50%) Examination
Components Class viva records Quiz Attendance
(Drop down) Test (50%)

Weightage (%) 20 10 10 5 5 50

Text /Reference Books:

 Textbook of medical physiology by Arthur C. Guyton and John E. Hall; Ed.11th; Saunders; 2005. ISBN 0-7216-0240-1
 Principles of Immunology and Immunodiagnostics, Ralph Michael Aloisi. Lippincott Williams and Wilkins ISBN-13, 9780812111330.
 Harper's Review of Biochemistry (29th edition) RK Murray, DA Benderer, PJ Kennelly, VW Rodwell and PA Weil. 2012, McGraw Hill Co.
ISBN-13 978-0071825344
 Elements of medical Genetics by Turnpenny and Ellard, Churchill Livingstone: ISBN: 9780702045059
 Laboratory Manual:
 Mosby's Manual of Diagnostic and Laboratory Tests, 6th Edition Kathleen Deska Pagana & Timothy J. Pagana ISBN : 9780323697033
 Wegner, R. D. (Ed.). (2013). Diagnostic cytogenetics. Springer Science & Business Media.
 2. Carl A. Burtis, Edward R. Ashwood and David E. Bruns (eds) (2007): Tietz Textbook of Clinical Chemistry and Molecular Diagnosis (5th edition). Elsevier
 3. McPherson, R. A., & Pincus, M. R. (2017). Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book. Elsevier Health Sciences.
 4. Coleman, W. B., & Tsongalis, G. J. (Eds.). (2006). Molecular diagnostics: for the clinical laboratorian. Springer Science & Business Media.
 5. Leonard, D. G., Bagg, A., Caliendo, A. M., Deerlin, V. M., & Kaul, K. L. (Eds.). (2007). Molecular pathology in clinical practice (pp. 411-424). Springer.

Bloom’s Remembering Understanding Applying Evaluating Analyzing
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Class Test   
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Class Test  
2

Home  
Assignment
(HA1)

Home    
Assignment
(HA2)

Quiz 1 

Quiz 2  

Quiz 3    