Ta i w a n R e d E y e B i o m e d i c a l I n c .

- From Lab to Life

Rapid test of crude palm oil DOBI

(Deterioration of Bleachability Index)
Crude Palm oil (CPO)
- edible oil derived from pulp of oil palms

Three main process to get pure and

refined oil from crude palm oil:
⚫ De-gumming process,
⚫ Bleaching process
⚫ De-odourising process.

http://www.palmoilmillplant.com/related-products/palm-oil-chemical-physical-refining-process.html
 Key quality parameters of crude palm oil: DOBI
(Deterioration of Bleachability Index)
Fruit bunches showing different ripeness
𝐴 446
DOBI=
𝐴 269
✓ A446 is the absorbance at 446 nm.
✓ A269 is the absorbance at 269 nm.

⚫ Crude palm oil quality control

⚫ Predict the ease of refining of crude
palm oil
⚫ Indicator for oxidative status
Use of the Deterioration_Of_Bleachability index to characterise the
quality of crude palm oil
Traditional method for DOBI determination -
spectrophotometer
 Handheld device for DOBI determination
- OilEye

Screen Probe is coated with

silica for protection.

Power button

Read button

Dual LED light source


Step 1. Push power button
to turn on the device
Step 2. Put the probe into blank solution gently
Step 4. The device will jump into
for 10 times to rinse the probe. Hold the Step 3. Calibration is Step 5. Wipe out excess
“Push R Key to test’ automatically
device and stand still in the blank solution for completed. solution on the probe.
for the following measurement.
10 sec. Press R button to start measurement.

The results shown can be

changed to show DOBI only:
➢ DOBI>2.31; Green icon
➢ 2.10 ≤ DOBI ≤ 2.31; Orange
icon
➢ DOBI <2.10; Red icon

Step 6. Put the probe into the sample

gently for 10 times to rinse the Step 7. Waiting for the Step 8. Result display (sample device) Step 9. To test different
probe. Hold the device and stand still result to display. Y1: 269nm Absorbance samples, press R and start
in the sample for 10 sec. Press R Y2: 446nm Absorbance from step 5 for measurement.
button to start measurement.
Sample preparation

1、Weigh 0.2g of filtered crude palm oil

2、Dissolve the sample in 50ml solvent (Iso-octane)
3、Measure the sample using OilEye in a 100ml beaker
Spectrophotometer for comparison
GIE Optics
Genesys 150 Mars HS2000+
 Spectrum measured from spectrophotometer
1.2
446nm

1.0

0.8

Abs
0.6 269nm

0.4

0.2

0.0
200 225 250 275 300 325 350 375 400 425 450 475 500

wavelength((nm)

The obtained spectra were used to create prediction models for quantification of the individual key parameters. The quality
of the prediction models was evaluated using correlation diagrams, which display the correlation between LED prediction
and primary method values.
 Absorbance extract from spectrophotometer
- Using different cuvettes
r-squared: 0.99 r-squared: 0.93

0.5 0.15

0.4
0.10

269nm Abs
0.3
446nm Abs

0.2 0.05

0.1

0.00
0.0
0.00 0.25 0.50 0.75 1.00 0.00 0.25 0.50 0.75 1.00

dilution dilution
 Absorbance extract from spectrophotometer
- Using same cuvette
r-squared: 0.99 r-squared: 0.95

0.5 0.15

0.4
0.10

269nm Abs
0.3
446nm Abs

0.2
0.05

0.1

0.00
0.0
0.00 0.25 0.50 0.75 1.00 0.00 0.25 0.50 0.75 1.00

dilution dilution
 Absorbance extract from spectrophotometer
r-squared: 0.99 r-squared: 0.99

1.0 0.3

0.8
0.2

269nm Abs
0.6
446nm Abs

0.4
0.1

0.2

0.0
0.0
0.00 0.25 0.50 0.75 1.00 1.25 1.50 0.00 0.25 0.50 0.75 1.00 1.25 1.50

dilution dilution
OilEye performance test - photoelectric conversion

18000 22000
16000 20000
14000 18000

12000 16000

Count
14000
Count

10000
12000
8000
10000
6000
8000
4000
LED1(269nm) 6000 LED2(446nm)
2000
4000
0 50 100 150 200 0 5 10 15 20 25 30
Current (mA) Current (mA)

r-squared: 0.999 r-squared: 0.999

OilEye performance test - stability

0.010
LED1(269nm)
0.008
LED2(446nm)
0.006
0.004
Absorbance

0.002 Test in ambient environment for 30 times:

0.000 System stability of absorbance is ±0.002.
-0.002
-0.004
-0.006
-0.008
-0.010
0 5 10 15 20 25 30
Number of time
 Absorbance extract from OilEye
r-squared: 0.99 r-squared: 0.99

1.6
0.5
1.4

1.2 0.4

1.0
446nm Abs

269nm Abs
0.3
0.8

0.6 0.2

0.4
0.1
0.2

0.0 0.0
0.0 0.5 1.0 1.5 2.0 2.5 0.0 0.5 1.0 1.5 2.0 2.5
dilution dilution
Performance comparison

GIE Optics RedEye

Genesys 150 (standard)
Mars HS2000+ OilEye 2
dilution
Absorbance Absorbance Absorbance Absorbance Absorbance Absorbance
DOBI DOBI DOBI
269nm 446nm 269nm 446nm 269nm 446nm

0.5X 0.111 0.321 2.88 0.097 0.312 3.21 0.097 0.312 3.21
1X 0.206 0.615 2.97 0.193 0.605 3.13 0.194 0.615 3.17
1.5X 0.307 0.894 2.90 0.298 0.896 3.00 0.293 0.915 3.12
2X 0.393 1.146 2.91 0.388 1.181 3.04 0.382 1.192 3.12
2.5X 0.485 1.383 2.84 0.473 1.46 3.08 0.479 1.437 3.00

The measurement result of OilEye is close to Genesys measurement result.

Correlation diagram of OilEye and Genesys
OilEye Rapid-test DOBI meter for crude palm oil (CPO)
Conventional spectrometer

DOBI inspection for ✓ Benchtop

QUALITY CONTROL ✓ Higher than $3500
✓ Complicated

• Farmer
OilEye spectrometer
• Buyer
• Processing plant

✓ Handheld
✓ Lower than $800
✓ Easy to use
OilEye appearance for production
(Model name: OilEye 2)
影片投影片
Welcome business partners!

Nelson Yan, Ph.D.

Founder & CEO
styan@redeyebmi.com
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