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    DSMZ Medium830

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    lidgen.iniqui.unsa
    This document provides the recipe for R2A medium, which contains yeast extract, proteose peptone, casamino acids, glucose, soluble starch, sodium pyruvate, potassium phosphate, magnesium sulfate, and agar. The ingredients are dissolved in distilled water and autoclaved at 121°C for 15 minutes to prepare the growth medium, which has a final pH of 7.2. A note indicates that for microorganism DSM 22224, half strength R2A liquid medium or agar should be used.

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    DSMZ Medium830

    Uploaded by

    lidgen.iniqui.unsa
    7 views1 page
    This document provides the recipe for R2A medium, which contains yeast extract, proteose peptone, casamino acids, glucose, soluble starch, sodium pyruvate, potassium phosphate, magnesium sulfate, and agar. The ingredients are dissolved in distilled water and autoclaved at 121°C for 15 minutes to prepare the growth medium, which has a final pH of 7.2. A note indicates that for microorganism DSM 22224, half strength R2A liquid medium or agar should be used.

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    DSMZ_Medium830

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    This document provides the recipe for R2A medium, which contains yeast extract, proteose peptone, casamino acids, glucose, soluble starch, sodium pyruvate, potassium phosphate, magnesium sulfate, and agar. The ingredients are dissolved in distilled water and autoclaved at 121°C for 15 minutes to prepare the growth medium, which has a final pH of 7.2. A note indicates that for microorganism DSM 22224, half strength R2A liquid medium or agar should be used.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    7 views1 page

    DSMZ Medium830

    Uploaded by

    lidgen.iniqui.unsa
    This document provides the recipe for R2A medium, which contains yeast extract, proteose peptone, casamino acids, glucose, soluble starch, sodium pyruvate, potassium phosphate, magnesium sulfate, and agar. The ingredients are dissolved in distilled water and autoclaved at 121°C for 15 minutes to prepare the growth medium, which has a final pH of 7.2. A note indicates that for microorganism DSM 22224, half strength R2A liquid medium or agar should be used.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Microorganisms

    830. R2A MEDIUM

    Yeast extract 0.50 g


    Proteose Peptone (Difco no. 3) 0.50 g
    Casamino acids 0.50 g
    Glucose 0.50 g
    Soluble starch 0.50 g
    Na-pyruvate 0.30 g
    K2HPO4 0.30 g
    MgSO4 x 7 H2O 0.05 g
    Agar 15.00 g
    Distilled water 1000.00 ml

    Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar,
    heat medium to boiling to dissolve agar, and autoclave for 15 min at 121C.

    For DSM 22224 use half strength R2A liquid medium or agar.

    © 2012 DSMZ GmbH - All rights reserved

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