Reagent Purpose Composition Handling Storage Disposal

Benedict’s Reagent For detecting -Sodium carbonate -Wear prescribed -Store in tightly - Dispose in a
simple sugar such (Na2CO3) PPE when handling closed containers. suitable, closed
as glucose. -Sodium citrate this substance. - Store in a cool, container.
(Na3C6H5O7) -Avoid skin and eye dry and - Label waste
-Copper(II) sulfate contact. well-ventilated the container
pentahydrate -Avoid ingestion and area. with the proper
(Cu(SO4)2 5H2O) inhalation -Store away hazard symbol.
-Wash hands storage from -In case of
thoroughly after incompatible spillage,
handling. substances. absorb with
-In case of skin and chemically
eye contact, rinse inactive
with cleaning running materials and
water. dispose in a
suitable
container.

Fehling’s reagent Identify the Mixture of two -Wear prescribed -Store in its original - Dispose in a
presence of solutions: PPE when handling container and suitable, closed
aldehydes and this substance. ensure that it is container.
ketones with Fehling A solution: -Familiarize safety tightly closed. - Label waste
corresponding -Copper sulfate precautions. -Store in a cool dry the container
functional groups to pentahydrate; -Avoid skin and eye and well-ventilated with the proper
identify the -Water contact. area. hazard symbol.
presence of (CuSO4 5H2O) -Avoid ingestion and -Store away -In case of
reducing sugars inhalation. storage from spillage,
and non-reducing Fehling B solution: -In case of skin and incompatible absorb with
sugars. -Potassium Sodium eye contact, rinse substances. chemically
Tartrate; with cleaning running -Keep away from inactive
-Water water. food and materials and
(KNaC4H4O6 4H2O) -Avoid releasing into beverages. dispose in a
the environment. suitable
container.
*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
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Tollens’ reagent To distinguish -Silver Nitrate -Wear prescribed -Store in its original -Dilute
aldehydes and (AgNO3) PPE when handling container and substance
ketones. -Sodium Hydroxide this substance. ensure that it is before disposal.
(NaoH) -Avoid skin and eye tightly closed. Small quantities
-Ammonia (NH3) contact. -Store in a cool dry can be
-Water (H2O) -Avoid ingestion and and well-ventilated disposed of
inhalation. area. down the sink,
-In case of skin and -Store away or in a proper
eye contact, rinse storage from waste container
with cleaning running incompatible with a proper
water. substances. hazard symbol.
-Avoid contact with -Protect from -In case of
metals to avoid sunlight exposure. spillage,
corrosion. absorb with
chemically
inactive
materials and
dispose in a
suitable
container.

*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
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Molisch’s reagent To check for the -Ethanol (C2H6O) -Wear prescribed -Store in its original -Dispose in a
presence of - 1-Napthol PPE before handling container and suitable, closed
carbohydrates in a (C10H7OH) this substance. ensure that it is container.
given analyte. -Avoid skin and eye tightly closed. - Label waste
contact. -Store in a cool, dry the container
-Avoid ingestion and and well-ventilated with the proper
inhalation. area. hazard symbol.
-In case of skin and -Store away from In this case,
eye contact, rinse flammable flammable.
with cleaning running substances and fire
water. hazards.
- Avoid exposure to
open flame, heat and
spark.
-In case of fire, use
suitable extinguishing
agents.

Nylander’s reagent To identify the -Potassium -Wear prescribed -Store in its original -Dispose in a
presence of Hydroxide (KOH) PPE before handling container and suitable, closed
reducing sugars. -Bismuth subnitrate this substance. ensure that it is container.
(BiONO3) -Avoid skin and eye tightly closed. - Label waste
-Potassium sodium contact. -Store in a cool dry the container
(+) tartrate -Avoid ingestion and and well-ventilated with the proper
(KNaC4H4O6 4H2O) inhalation. area. hazard symbol.
-In case of skin and -Store away -Contact
eye contact, rinse storage from professionals to
with cleaning running incompatible facilitate
water. substances. disposal.
-Avoid contact with -Keep away from
zinc, aluminum, and food and
tin. beverages.

*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
***FOLLOW PRE & POST LABORATORY PROCEDURES.***
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Schiff’s reagent For detection and -Fuchsin dye -Wear prescribed -Store in its original -Dispose in a
identification of (C2OH19N3.HCl) PPE before handling container and suitable, closed
aldehydes and -Sodium bisulfite this substance. ensure that it is container.
ketones (NaHSO3) -Familiarize safety tightly closed. - Label waste
-Hydrochloric acid precautions when -Store in a cool dry the container
(HCl) handling this and well-ventilated with the proper
substance. area. hazard symbol.
-Avoid skin and eye -Store away -Do not dispose
contact. storage from in sinks to avoid
-Avoid ingestion and incompatible corrosion.
inhalation. substances. -In case of
-In case of skin and spillage,
eye contact, rinse absorb with
with cleaning running chemically
water. inactive
materials and
dispose in a
suitable
container.

Millon’s reagent To detect the -Mercuric nitrate -Wear prescribed -Store in its original -Dilute
presence of (Hg(NO3)2) PPE before handling container and substance
tyrosine, an amino -Nitric acid (NHNO3) this substance. ensure that it is before disposal.
acid containing -Water (H2O) -Avoid skin and eye tightly closed. Small quantities
phenol. contact. -Store in a cool dry can be
-Avoid ingestion and and well-ventilated disposed of
inhalation. area. down the sink,
-In case of skin and -Store away or in a proper
eye contact, rinse storage from waste container
with cleaning running incompatible with a proper
water. substances. hazard symbol.
-Keep away from -In case of
metallic materials. spillage,
absorb with
chemically
*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
***FOLLOW PRE & POST LABORATORY PROCEDURES.***
*****READ MATERIAL SAFETY DATA SHEET ON HAND*****
 inactive
materials and
dispose in a
suitable
container.

Lugol’s reagent -Starch indication Potassium Iodide -Wear prescribed -Store in its original - Dispose in a
and used in (KI) PPE before handling container and suitable, closed
Gram-staining. Iodine (I) this substance. ensure that it is container.
-To differentiate Water (H2O) -Avoid skin and eye tightly closed. - Mark waste
protozoa and cysts contact. -Store in a cool dry container with
in fecal samples. -Avoid ingestion and and well-ventilated the proper label.
-Used for thyroid inhalation. area. -In case of
treatments -In case of skin and spillage,
eye contact, rinse absorb with
with cleaning running chemically
water. inactive
materials and
dispose in a
suitable
container.

Biuret reagent -Detect protein and Cupric sulfate -Wear prescribed -Store in its original -Dispose in a
peptide bonds. (Cu(SO4)2) PPE before handling container and suitable, closed
Potassium Iodide this substance. ensure that it is container.
Potassium sodium -Avoid skin and eye tightly closed. - Label waste
tartrate contact. -Store in a cool dry the container
(NaKC4H4O6) -Avoid ingestion and and well-ventilated with the proper
Sodium hydroxide inhalation. area. hazard symbol.
(NaOH) -In case of skin and -Store away from -Contact
eye contact, rinse oxidizing agents, professionals to
with cleaning running acids, and bases. facilitate
water. disposal.
-Keep away from
metallic materials.
*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
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Hopkin’s cole -Used to detect the Glyoxylic acid -Wear prescribed -Store in its original - Dispose in a
presence of (C2H2O3) PPE before handling container and suitable, closed
tryptophan in this substance. ensure that it is container.
proteins. -Avoid skin and eye tightly closed. - Label waste
contact. -Store in a cool dry the container
-Avoid ingestion and and well-ventilated with the proper
inhalation. area. hazard symbol.
-In case of skin and -Store away from -In case of
eye contact, rinse oxidizing agents, spillage,
with cleaning running acids, and bases. absorb with
water. chemically
inactive
materials and
dispose in a
suitable
container.

Guaiac reagent -Used in detection Ethyl alcohol -Wear prescribed -Store in its original -Dispose in a
of occult blood in (CH3CH2OH) PPE before handling container and suitable, closed
fecal samples. Isopropyl Alcohol this substance. ensure that it is container.
(CH3CHOHCH3) -Avoid skin and eye tightly closed. - Label waste
Methyl Alcohol contact. -Store in a cool, dry the container
(CH3OH) -Avoid ingestion and and well-ventilated with the proper
Water (H2O) inhalation. area. hazard symbol.
Gum Guaiac -In case of skin and -Store away from In this case,
eye contact, rinse flammable flammable.
with cleaning running substances and fire
water. hazards.
- Avoid exposure to
open flame, heat and
spark.
-In case of fire, use
suitable extinguishing
agents.

*NOTE: DISPOSAL SHOULD BE SUPERVISED BY PROFESSIONALS. DO NOT DISPOSE IN REGULAR TRASH BINS.*
***FOLLOW PRE & POST LABORATORY PROCEDURES.***
*****READ MATERIAL SAFETY DATA SHEET ON HAND*****