Medical Chemistry

Branch of Basic Sciences

Report: Determine the percentage of active substance in
aspirin
EXPERIMENT: 4

Basra University | Dentistry College

Team lab names: Mariam Kadhim, Zainab Hussain, Fadak al-Zahraa

Humam, Shahad Adel
Group: 2
Experiment Date: Tuesday 2023/1/2
Experiment moderator: A.T Sultan Al-Bakheet
ABSTRACT: This experiment was conducted to determine the
determine the percentage of the active component in Aspirin as an
s“titration” process practically
INTRODUCTION: Aspirin is anti-inflammatory drug used to reduce
pain, fever, and/or inflammation, and as an antithrombotic. Aspirin
is also used long-term to help prevent further heart attacks,
ischaemic strokes, and blood clots people at high risk.
The active ingredient in aspirin, and the chemical for
which aspirin is the common name, is acetylsalicylic acid (is a
weak acid) and also include inactive ingredients that help
produce a consistent product. Aspirin Trade names: Bayer
Aspirin, Other names: 2-acetoxybenzoic acid acetylsalicylate
acetylsalicylic acid. Different strengths of aspirin are based on
the amount of acetylsalicylic acid present.
In this experiment, we will measure this amount by
using a quantitative technique known as titration. A titration
is a procedure for determining the concentration of a solution
(the analyte) by allowing a carefully measured volume of this
solution to react with another solution whose concentration is
known (the titrant).
The point in the titration where enough of the titrant
has been added to react exactly with the analyte is called the
equivalence point, and occurs when moles of titrant equal
moles of analyte according to the balanced equation.
Number of moles of OH = Number of moles of HA
Chemical indicator, any substance that gives a visible sign, usually
by a color change. An example is the substance called phenolphthalein
The color of the ph.p solution depends upon the pH range of the solution.
MATERIALS:
• Dropper
• Graduated cylinder
• Funnel
• Beaker (50 ml)
• Erlenmeyer flask
• NaOH (sodium hydroxide) solution (aq)
• Aspirin grinded powder
• Phenolphthalein solution (the indicator)
• Burette
• Sensitive electronic balance
• Ethanol solution (aq)
METHOD:
1.We record the exact molarity of NAOH (aq), which is labeled on
the stock bottle.
2.rinse the inside of the burette with distilled water.
3.Now rinse the burette with a small amount of NaOH (aq)
4.Fill the burette with NaOH (aq) up to the top, now measure the
volume at the level of the NaOH precisely, and record it as the
“Initial
Burette Reading on your report.
5.Find the mass of an aspirin tablet using the electronic balances.
Place this aspirin tablet in a 250mL Erlenmeyer flask.
6.Add 20mL of distilled water to the Erlenmeyer flask.
7.Wait around 30 seconds for the aspirin tablet to swell and fall
apart. After the tablet falls apart, add 20mL of ethanol to dissolve
the aspirin.
8.Swirl the solution to ensure that it is homogenous. There is likely
to be undissolved binder remaining in the flask.
9.Add 5 drops of phenolphthalein indicator to the flask.
10.Begin the titration by slowly adding NaOH (aq) from the burette
to the aspirin solution in the Erlenmeyer flask.
11.Swirl Erlenmeyer flask as you add the base in order to efficiently
mix the chemicals.
12.Eventually will turn the solution in the Erlenmeyer flask a pale
pink color that does not disappear when swirled. This indicates that
the equivalence point has been reached.
13.Do not add any more NaOH (aq) at this point. Measure this
volume of NaOH (aq) precisely as “the final burette reading”.

RESULTS:
calculation
V1s=21 V=33-21
12
V2=33 V=12ml→ = 0.012𝐿
1000

Equivalent point = Number of moles of OH = Number of moles of HA

n(H+)=M.VL
n(H+)=0.0012 mol
𝑊
𝑛= → 𝑊 = 𝑛(𝐻 + ). 𝑀𝑊
𝑀𝑊
W=0.0012*180=0.216 g
0.0216
%= × 100→ %=216%↓
0.1
DISCUSSION:
The purpose off adding sodium hydroxide was for measuring the active
component in aspirin (acetylsalicylic acid) which react with hydroxide in
process called “equivalent reaction”. The number of hydrogen
molecules that react equal to the number of the hydroxide molecules.
We took a small amount of aspirin nearly (0.15 g) and dissolves in water
and ethanol, some amount of aspirin didn’t’t dissolve in the solution.
In this situation we needed to add an indicator which inform us about
the equivalence point (E.P).
We measure the amount of sodium hydroxide precisely as it drops from
the burette
the phenolphthalein turns to faint pink as we add sodium hydroxide
from the buret which means we reach the endpoint of the reaction
the calculation might be inaccurate due to the confusion in the
measurement of aspirin weight