- The document discusses various bacteria, the diseases they cause, and tests used to identify them. It provides information on gram staining results, growth characteristics, pathogenic bacteria that cause diseases like typhoid fever, meningitis, food poisoning and more. It also lists various selective media and the bacteria they are used to isolate, such as TCBS agar for Vibrio cholerae.
- The document discusses various bacteria, the diseases they cause, and tests used to identify them. It provides information on gram staining results, growth characteristics, pathogenic bacteria that cause diseases like typhoid fever, meningitis, food poisoning and more. It also lists various selective media and the bacteria they are used to isolate, such as TCBS agar for Vibrio cholerae.
- The document discusses various bacteria, the diseases they cause, and tests used to identify them. It provides information on gram staining results, growth characteristics, pathogenic bacteria that cause diseases like typhoid fever, meningitis, food poisoning and more. It also lists various selective media and the bacteria they are used to isolate, such as TCBS agar for Vibrio cholerae.
- The document discusses various bacteria, the diseases they cause, and tests used to identify them. It provides information on gram staining results, growth characteristics, pathogenic bacteria that cause diseases like typhoid fever, meningitis, food poisoning and more. It also lists various selective media and the bacteria they are used to isolate, such as TCBS agar for Vibrio cholerae.
- case include a women with cat Toxoplasmo - resestins vancomysin ؟enterococcus fescium - Causes ammonia smell? Infection with E.coli - Normal flora in eyes ? s.epidermis - bacteria produce H2s? salmonella - movement of bacteria by ? fallagate - What bacteria cause HUS ? E.coli , shigell - Oxidate positive bacteria? Vibiro ,Pseudomonas, Neisseria, Alcaligens, Aeromonas, Campylobacter, Brucella, Pasteurella, Moraxella, Helicobacter pylori, Legionella,pneumophila - bacteria cause chronic pulmonary disease ? mycobacterium tubrcolesis - Patient with diarrhea we do selanit broth and we find H2S what is the bacteria cuses that ? Salmonell - H. influenzae ? Chocolate agar - bacteria from newborn infant, gows at 4 c ? listeria - which of the following is test to differentiate between staph aureus and micrococcus ? Oxidation and fermentation - Bacteria sensitive to opotichin ? Streptococcus pneumoniae - bacteria resistance to vancomycin ? Enteroccus faecalis - Antibiotics inhibit gram + ve ? Vancmycin - Typhoid and fever ? salmonella typhi - B. hemolytic in blood agar no growth in mackonky catalase+ ve ? s.aurus - blue green coloni ? Psedomonase due to pigmintation - cause meningitis for neonatal ? E.coli - bacteria found in young females ? S. Saprophytics - duble zone beta hemolysis? Colstridium , s. Pyogens - most commoncause UIT ? stahylococooous saprophyticus - diphtheria staine ? Albert stain - selective media v.cholera? TCBS - bacteria 4C ? Listeria - diarrhea of rice ? Bacillus cerus - media for n.gonorrhoea ? thayer martin agar - Mannitol salt agar ? s.sapro - bacteria cause food poisoning ? s.aureus - Sputum culture on agar ? Bloid agar , choclet agar - .H2S produced ? Sallomenella - Bacteria cause obortion ? Niesseria & listeria - Indol +, Methyl red +, VP -, Citrate - ? E. Coli - bacteria ureas possitive in stool ? Proteus - Zile _ Neelsen stain Acid Fast ?Myobacteria - Optician test ? Senstive : S,pnemonea , Resestens : S, virdens - Replasing fever ? Borrelia recurrentis - resistant to penicillin? Mycoplasmas - Rice watery stool can caused by ? Vibro cholera - Which media is used to isolate vibrio cholera? TCBS - Which test is used to differentiate between group B and A streptococcus? CAMP test - What bacteria can you get from a cat bite? Pasteurella , Yersinia pestis - UTI (urinary tract infections) is caused by? - saprophyticus - Meningitis is caused by the fungus ? Cryptococcus neoformans - Diffrent test between s.pyogens and other strepto ? Bacteracin sensitive - bacteria motile cause diarrhea ? Salmonella - bacteria need CO2 carbon dioxide and chocolates? Neisseria. - bacteria cause food poisoning ? s.aureus - Lowenstein- Jensen media ? Mycobacterium tubrcoloiss - resistant to penicillin? Mycoplasmas - stathorea ? حلطصمFat - Catalase pos oxidase pos gram positive cocci? Micrococus - HIV+ meningitis ? tuberculosis mycobacterium - Tonsillitis caused by ? s.pyogen - Endocarditis ? staph aureus - Catalase_ ve and b hemolytic ? s.pyogen/s.group B - hemolytic catalase +ve coagulase + ve grame +ve in cluster ? staph aureus - Hemolytic uremic syndrome caused by ?E.coli 0157 Note ; 0157 = serotype - Sputum sample cultured in chocolate agar under microscope appear filaments ?h.influenzae - Indian ink in case of bacteria capsule: klbssela - Watery diarrhea ? shigella. - Rice watery diarrhea ? vibrio cholerae هدنع يركس ضيرمulcer ؟ داوس ةلماع لامشلا مدقلا يفHav gas gangrene caused by Cl.perfringens - Patient with otitis media bacteria that causes ? S.aureus - ةنيع انلش نا سيكsynovial fluid ؟ هعقوتملا ايريتكبلاstrepto pyogene صافم باهتلا يوست اهنال - Sample culture in anaerobic condition ? Eye Swab - sample culture in charcoal media ? legionella - Septecemia ? sallmonella - Peptic ulcer/urease positive/grow in chocalte? h .pylori - Cultur of sputum And stain with weak acid fast bacilli ?nocardia - whooping cough caused ? bordetella pertussis - B. anthrax cause ? wool sorter disease a type of pneumonia - zoonotic diseases ? yersinia - vibrio on TCBS yellow colonies due to ? fermentation of sucrose - Non glucose ferment ? pseudomonas - lactose fermenter ( red to pink ) e.g : E.coli , Klebsilla , Enterobacte - Non lactose fermenter (colorless) e.g proteus , salmonella , shigella - selective media for bordetella ? bordetella gengou agar - shigella on Xld agar ? red colored or colourless withoutindicator - shigella on macconkey agar ? colourless with small colony - Salmonella in XlD ? pink colour with black center(ParatyphiB) - salmonella on macconkey agar ? colourless or pale yellow - Bacillary dysentery causes by ? shigella - Capsulated bacteria ? S.pnemonea , Nasieria , klbesila pnemonea , H.influenzae, Sallmonella typhi, C.neformans, psedomonas areganosa - Food poisoning causes by ? salmonella enteritidis - septicemia and gas gangrene caused by ?Clostridium perfringens - Lowfer serum media for ? Corynebacterium diphtheriae - .colour of gram positive bacteria ? violet colour - colour of gram negative bacteria ? red colour - Fishy smell in media characteristic for ? Proteus - To deferential between group B and A streptococcus ?CAMP test - Group A and B.Streptococcus ? B.hemolytic - prokaryote like bacteria. - Sore throt cussed by ? S.pyogens - Shagilla ? Non lactose ferment , non motile - Patient with severe borns and infections due to infection, what will appear in the microbiological examinations? P.aeruginosa - trapel sugar iron in agar A/K+ ve? Salmonella, Shigella and protein - The most common causes of food poisoning? clostridium perfringens - Bacteria from a newborn grows at 4 C? Listeria monocytogenes - What organisms can grow on agar Makconcy ? S. aureus - Bacteria no cell wall ? Mycoplasma bacteria - Gram + ve , motile , catalese -ve ? Listeria - Stain Alberat ? C. Diphatheria - Untreated bacteria cause stomach cancer? H.pylori - bacteria apper grenn metalic sheen on EMB agar ? E.coli - elek test ? C.diphtheria - 4-year-child is sick with meningitis, what are the bacteria that cause this? H.influnezaB - cholera oxidase give us? postive - Bacteria grow > 43 C ? Campelobacteri jejuni - patient have contamination after draw why? S. epidermes - Patient came from African , he has sleep sick , what probably seen in lab ? T.Bruci - dignosis test for suggestion bacteria ? Cultur - H . Influenza : gram negative cocobacill - Nessirea : gram postive dibcocoi - Bacteria can cause POISON ? ⁃ Clostridium perfringins⁃ Clostridium botulinum⁃ Staph.aureus⁃ Vibrio spp v.colorae , v.parahaemolylicus⁃ Bacillus cereus⁃ salmonella⁃ Shigella⁃ E.coli.Toxiginc⁃ Campylobacter⁃ Yersinie⁃ Listeria⁃ Aeromonas - Bacteria appear on blood agar like swarming? Protease. - Bacterial oxidase positive? Pseudomonas, Campylobacter, Vibrio, Helicobacter pylori - McConkey agar was used? Gram-negative bacilli but if you see in the answers (gram-negative Cocci), choose it - Catalase +ve motiliy +ve ? Listeria - Sallmonella and shagella ? Colorless - bacteria motile cuses diharrheia ? Sallmonella - Bacteria give beta hemolysis in blood agar ? S.aures - Bacteria motile by ? Flagella - The most bacteria causes toxic ? C.perfnges - What is the color of gram negative bacteria after staine ? Red color - What is the color of gram postive bacteria after staine ? Blue - Frist primary stain of asid fast stain ? Carbol fuschin - steps of gram stain : crystal violt (purple), iodine(mordent),Alchol(decolorastion),safrain (conterstain) - Steps of Acid fast stein ) : Carbol fuschin , het fix cell, Alchol(decolorastion),methaylblue (conterstain) - Thermophilic : 42C-80C - Microaerophic : Grows best in the presence of a low level of oxygen - kills with higher concentrations of oxygen eg Campylobacter spp., Helicobacter spp - Capenophilic : Requires high levels of carbon dioxide eg Neisseria spps - Kinyoun Ziehl Nielson : mycobacteria - Asid fast stain give coloo of gram + ? Pink - Asid fast stain give coloo of gram - ? Blue - Media Loeffer agar ? Crynobacteria - alkaline peptone ? V. Choloria - Buffered Charcoal Yeast Extract (BCYE) Agar ? Legionella pnemonea - indicote citrat test : bromthmol blue - Indicater indol test : kovac’s ( p-di mythayl amonia benzaldehyed ) - Voges Proskauer test Reagents: A: 5% alpha-naphthol, ethanol B: KOH, deionized water - Catalase test : postive Staphyl , negative strepto - Oxidaes test : end prducat color : blue + - Cogulase test : + s.aures - Bile esculine : E.facalis - No cell wall : mycoplasma , ureaplasma - Chlamydi : trachomatis - Borrelia burgdorferi : relapsing fever - Treponema pallidum : syphilis - Dignosis syphilis : confirmatory ( FTA-ABS), specific test ( RPR ) ( TPHA) , scrining test ( VDRL) , dark field microscopy. - Screw like bacteria ? syphilis - Cuses lesion on penis ? syphilis - Growth on artfical media ? Spirochate (syphilis) - Gram postive cocci : grap like in culster - S.aures ? Colonies are golden color beta hemolytic on blood agar - S.epdermidis : prosthetic implant infection - S.saprophytics: UTI in old women - ASO tes postive and CRP postive ? S. Pyogens - RF ? S. Pyogens - C.diphtheria : psedomonas at tha backof the throat - Dobel Zone : C.perfringes - Neurotoxien and trminal spor : C.tetani - Neurotoxien : C.botulinum - Diplococco: niseeria - Bacteria meningitis : ⬆ ️neutrophils⬆ ️protien ⬇ ️glucose - Bacillus anthracis : biological warfare - Bacillus anthracis : woolsorters deaseas - Bacillus anthracis : polychroma MB stain capsule - Klebisell and shigell and yersinea : motile - Non lactos ferment : shigell and sallmonel and yarsines and protees - Lactose ferments : E.coli ,klpsilla , seerita , citrobacteria,enterobacteria - Watery diarrhea in chliidern : EPEC - Travel diarrhea : ETEC - Seritea : ONPG agar : red pigment - S.typhi: Typhoid fever - Camylobacteria jejuni : gastroenteritis - H. influnzea : Facor V Factor X - H. influnzea : meningitis infant - Specimin urin : SBA media 0.01ml or 0.001 ml claprated loop - Specmin Blood : blood to broth 1:5 to 1:10 - Steralztion : Autoclave 121C at 15pci - Antibiotic Prootine synthesis inhiboter ? Aminoglycosides - Antibiotic inhibioter RNA ? Rifampicn - Antibiotic inhibioter DNA ? quinolones - MIC : The lowest concentration of the antibiotic prevents the growth of bacteria as well as does not harm the patient (bacteriostatic ) - MBC : The lowest concentration of the antibiotic kills the bacteria as well as does not harm the patient (bactericidal) - Brucella melitensis : Malta fever - Bordetella pertussis : whooping cough - MIC high : Antagonism - MIC low : synergism - Meningitis noenatel : S.agalactiae , L. Monocytogens, E.coli - Meningitis 6month to 6 years : S.pnemonea,H.inflonzea type B , Nessirea - Meningitis 6years to 60 years : S.pnemonea , Nessirea