Hemoglobina

• The observation of crystalline hemoglobin was first reported by Friedrich

Hünefeld in 1840, and by 1909 Edward Reichert and Amos Brown had
published a photographic atlas of hemoglobin crystals from several hundred
species.

• In contrast, it was not until 1926 that crystals of an enzyme, those of jack
bean urease, were first reported.

• Hemoglobin was one of the first proteins to have its molecular mass
accurately
determined;

• The first protein to be characterized by ultracentrifugation;

• The first to be associated with a specific physiological function (that of oxygen

transport), and, in sickle-cell anemia, the first in which a point mutation was
demonstrated to cause a single amino acid change.
• The first protein X-ray structures to be elucidated were those of hemoglobin
and myoglobin.
Hemoglobina: Proteína Honorária

Não é um tanque de oxigênio, mas uma sofisticada

estrutura funcional que transporta e entrega oxigênio
de forma modulada com o pH da sangue e outras
condições de regulação.
 Mioglobina
Reserva O2 (mamíferos aquáticos).
Fornece oxigênio rapidamente
para o músculo em atividade
(difusão lenta a partir dos
capilares).
Metabolismo NO ..
65-kD heterotetrâmero, a2b2
O Papel da Globina
Microambiente
Previne auto-oxidação do
oxiheme.
Possibilita modulação da
afinidade pelo oxigênio
molecular pelo pH do sangue
(Efeito Bohr), pCO2 e [Cl-] e
2,3 difosfoglicerato.
Perhaps the most elegant model for
describing cooperative ligand binding
to a protein is the symmetry model of
allosterism, which was formulated in
1965 by Jacques Monod, Jeffries
Wyman, and Jean-Pierre Changeux.
This model, alternatively termed the
MWC mode.