UV Transilluminator

BK-UA2
User Manual

BIOBASE GROUP

Version 2021.05
 Function overview
The function of the ultraviolet analyzer is to be used for fluorescence analysis: when a material
molecule in the ground state is exposed to excitation(ie, ultraviolet light), it absorbs the excited
energy and the material molecule is in the excited state.
Technical indicators
Filter transmission: 200×150mm, Reflection: 200×80mm
Transmission light source: 302nm, reflection on both sides: 254nm, 365nm
U.v. tubes: 10, white light 40 w
Operation steps
1. Gel study if it is, it is recommended that the selection of wavelength of 302 nm(UVB), please
according to 302 nm (UVB)wavelength switch selector will open at this time, observation sample
preparing gel strength, is used in the preparation of observation and cutting operations, such as to
reduce the ultraviolet (uv) on DNA damage, at this time, please open the wavelength switch
365(UVA) nm and 254 (UVC)nm. Choose the appropriate wavelength, you can for observation,
analysis, and cutting operations such as gel.
2. Samples will be good gel electrophoresis or thin layer, paper sample placed in the middle of the
instrument, open power supply, to make the sample the most clear and bright fluorescence. Usually
reflect ultraviolet as close to the sample, the fluorescence. Boot for about three minutes, it is the best
time to watch and take pictures, don't miss.. If you need to take a photo, place the digital camera at
the camera mouth, adjust the aperture and focal length to start the photo.
3, such as the need to slice, with his hands into the cut on either side of the gate can be performed.
4 and transformation by ultraviolet fluorescence, the fluorescence directly to the board on the
ultraviolet light boxes, switch between ultraviolet and visible light can be realized and applicable to
the analysis of protein gel.
5, install a photographic photograph floorstand and fixed screw. As long as the camera fitted with a
filter. Put the camera in camera middle round hole, when the camera take pictures after stability.

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