Summary of Sampling Requirements for Inorganic Parameters

Minimum Volume of
Parameters Container Material Mode of Preservation
Sample
Plastic/Polyethylene of
1 Antimony Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3

Plastic/Polyethylene of
2 Arsenic Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3
Plastic/Polyethylene of
3 Barium Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3

Polyethylene bottles or 100 mL (Fill container Store all reagents in

4 Boron alkali-resistant, boron- completely to exclude polyethylene or boron-
free glassware air) free containers

Fill container completely

to exclude air

Plastic/Polyethylene of
5 Cadmium Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3

Plastic/Polyethylene of
6 Chromium (Total) Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3

Add NaOH to pH>12.

Dark Polyethylene/Plastic
7 Cyanide (Total) 500 mL Remove sulfide
or Glass Bottle
Refrigerate in the dark

Preferred: Polyethylene
8 Fluoride 200 mL None required
bottles

Glass bottles if bottle

does not contain high-
fluoride solutions
Polytetrafluoroethylene
(PTFE) containers

Plastic/Polyethylene of
9 Lead Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3

Acidify sample at the

Acidified Polyethylene
10 Manganese 1 liter time of collection with
bottle
NHO3 to pH <2
 Glass container rinsed Add HNO3 to unfiltered
11 Mercury (Total) 500 mL
with 50 % HNO3 sample to pH <1

Add K2Cr2O3

12 Nickel Plastic or Glass bottle 500 mL Add HNO3 to pH < 2

Glass or
Refrigerate (unfiltered
13 Nitrate Plastic/Polyethylene 500 mL
sample)
container

Filter on site (0.45

cellulose acetate
membrane filter and
freeze)

Glass or
Colorimetric Method: Freeze at -20degC or
14 Nitrite Plastic/Polyethylene
50 mL sample store at 4degC
container

Plastic/Polyethylene of
15 Selenium Glass containers rinsed 100 mL Add HNO3 to pH < 2
with 50 % HNO3
 Holding Time

28 days

28 days

28 days

28 days

28 days

28 days

24 hours

28 days

28 days

28 days
28 days

6 months

24 hours

1 month-consult
analyst depending on
analytical method

1 to 2 days

28 days
Summary of Sampling Requirements for Organic Parameters
Minimum Volume of
Parameters Container Material
Sample
Industrial Pollutants
1. Benzene Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum

Amber glass bottles with a

2. Benzo(a)pyrene screw cap lined with TFE 1 liter

3. Carbon Tetrachloride Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

4 1,2-Dichlorobenzene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

5 1,4-Dichlorobenzene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

6 1,2-Dichloroethane Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

7 1,2-Dichloroethene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum
 Screw-cap vial with a hole
in the center and TFE-faced
silicone septum

8 Dichloromethane Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

9 Di(2-ethylhexyl)phthalate Amber glass bottles with a 1 liter

screw cap lined with TFE

10 Ethylbenzene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

11 Styrene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

12 Tetrachloroethene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

13 Toluene Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

14 Vinyl Chloride Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum
 Screw-cap vial with a hole
in the center and TFE-faced
silicone septum

15 Xylenes (total) Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

Pesticides
1. Aldrin and Dieldrin Amber glass bottles with a 1 liter
screw cap lined with TFE

2. Atrazine Amber glass bottles with a 1 liter

screw cap lined with TFE

None specified

Amber glass bottles fitted

with
polytetrafluoroethylene
3. Carbofuran (PTFE)-lined screw caps.

4. Chlordane Amber glass bottles with a 1 liter

screw cap lined with TFE

5. 1,2-Dibromo-3-chloropropane (DBCP) Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum
6. Dichlorodiphenyltrichloroethane (DDT) Amber glass bottles with a 1 liter
screw cap lined with TFE

7. Endrin Amber glass bottles with a 1 liter

screw cap lined with TFE

Ethylene Dibromide or 1,2- Screw-cap vial with a hole 2 x 40 mL

8. Dibromoethane in the center and TFE-faced
silicone septum

Polypropylene or amber
glass ontainer 500 mL
representative
9. Glyphosate sample
10. Lindane Amber glass bottles with a 1 liter
screw cap lined with TFE

Disinfection Chemicals and By-Products

1. Acrylamide Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum

2. Epichlorohydrin Screw-cap vial with a hole 2 x 40 mL

in the center and TFE-faced
silicone septum

3. Chlorine Dioxide None specified None specified

500 mL
Plastic (polyethylene or
equivalent) or Glass
4. Chlorine Residual container
 500 mL
Glass or
Plastic/Polyethylene
5. Bromate container

500 mL
Glass or
Plastic/Polyethylene
6. Chlorate container

Glass or
Plastic/Polyethylene
7. Chlorite container 500 mL

8. Dibromoacetonitrile Glass vial with TFE-lined 40 mL

screw caps

9. Dichloroacetate 25 or 40 mL
Screw-cap vial with a hole (depending on vial
in the center and TFE-faced used)
silicone septum; zero
headspace
10. Dichloroacetonitrile Glass vial with TFE-lined 40 mL
screw caps

25 or 40 mL
Screw-cap vial with a hole (depending on vial
in the center and TFE-faced used)
silicone septum; zero
11. Monochloroacetate headspace
25 or 40 mL
Screw-cap vial with a hole (depending on vial
in the center and TFE-faced used)
silicone septum; zero
12. Trichloroacetate headspace

13. 2,4,6-Trichlorophenol
14. Bromoform
15. Bromodichloromethane (BDCM)
16. Chloroform
17. Dibromochloromethane (DBCM)
25 or 40 mL
Screw-cap vial with a hole (depending on vial
in the center and TFE-faced used)
silicone septum; zero
headspace
Amber glass bottles with a 1 liter
screw cap lined with TFE
Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum
Glass bottle sealed with 1 liter
TFE-lined screw caps
Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum
Glass bottle sealed with 1 liter
TFE-lined screw caps
Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum
Glass bottle sealed with 1 liter
TFE-lined screw caps
Screw-cap vial with a hole 2 x 40 mL
in the center and TFE-faced
silicone septum
Glass bottle sealed with 1 liter
TFE-lined screw caps
 Mode of Preservation Holding Time
ollutants
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)

* For samples that contain residual chlorine, add

1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate

* Cool, ≤6⁰C 7 days until

extraction; 40
* For samples that contain residual chlorine, add days after
1000 mg ascorbic acid/L or 0.008% sodium extraction
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)

* For samples that contain residual chlorine, add

1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 7 days until
* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
 * For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate

des
* Cool, ≤6⁰C 7 days until
* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 7 days until

* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Add a sufficient amount of potassium

dihydrogen citrate to yield a concentration in the
sample of 9.2 to 9.5 g/L to prevent hydrolysis of
oxamyl, 3-hydroxycarbofuran, carbaryl, and
methiocarb

* Add sodium thiosulfate to yield a sample

concentration in the range of 80 to 320 mg/L to
eliminate residual chlorine
* During transport: Ice, temperature should not
exceed 10 degrees Celsius during the first 48 hours
after collection
* In the laboratory: Store samples at temperature
below 6 degrees Celsius and protect from light
until extraction. Do not freeze sample.

* Cool, ≤6⁰C 7 days until

* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
 * For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 7 days until
* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 7 days until

* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents

but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Add 100 mg/L sodium thisulfate for chlorinated 14 days
water and store at 4⁰C away from light

* Cool, ≤6⁰C 7 days until

* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

ls and By-Products
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
* Cool, ≤6⁰C 14 days
* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Analyse immediately -
Analyse immediately. Keep out of direct sunlight.

5 minutes
Refrigerate (unfiltered samples) 24 hours

Filter on site (0.45 µm cellulose acetate membrane 1 month-Consul

filter and freeze analyst
depending on
analytical
method

Refrigerate (unfiltered samples) 24 hours

Filter on site (0.45 µm cellulose acetate membrane 1 month-Consul

filter and freeze analyst
depending on
analytical
method

Refrigerate (unfiltered samples) 24 hours

Filter on site (0.45 µm cellulose acetate membrane 1 month-Consul

filter and freeze analyst
depending on
analytical
method

Cool, ≤6⁰C 14 days

-1 gram/ 60-mL amber glass vial (1% sodium
phosphate dibasic/99% potassium phosphate
onobasic + 0.6% ammonium chloride)
Refrigerate at 40⁰C 14 days or 21
days for sample
extracts freeze at
-11⁰C

Cool, ≤6⁰C 14 days

-1 gram/ 60-mL amber glass vial (1% sodium
phosphate dibasic/99% potassium phosphate
onobasic + 0.6% ammonium chloride)
Refrigerate at 40⁰C 14 days or 21
days for sample
extracts freeze at
-11⁰C

Refrigerate at 40⁰C 14 days or 21

days for sample
extracts freeze at
-11⁰C
Refrigerate at 40⁰C 14 days or 21
days for sample
extracts freeze at
-11⁰C

* Cool, ≤6⁰C 7 days until

* For samples that contain residual chlorine, add extraction; 40
1000 mg ascorbic acid/L or 0.008% sodium days after
thiosulfate extraction

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Chill to 4⁰C 14 days

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Chill to 4⁰C 14 days

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Chill to 4⁰C 14 days

* Cool, ≤6⁰C 14 days

* For samples that contain volatile constituents
but do not contain residual chlorine, add HCl to pH
<2.0 (4 drops, 1:1 HCl)
* For samples that contain residual chlorine, add
1000 mg ascorbic acid/L or 0.008% sodium
thiosulfate
Chill to 4⁰C 14 days