Lycopene Content in Tomato (Solanum lycopersium) Seed Extract as the

Main Ingredient in Making an Organic Anti-wrinkle Serum.

A Science Research Proposal

(APPLIED SCIENCE)

Presented to:
MRS. JANE CRYSTAL B. BAYAS
Science Research II Teacher

Presented by:

DAZZLE JEAN B. ALCORDO

Researcher
Grade 8-Anthurium

School Year 2020-2021

GOVERNOR FERRER MEMORIAL NATIONAL HIGH SCHOOL- MAIN

Pinagtipunan, City of General Trias, Cavite
 TABLE OF CONTENTS
PRELIMINARIES
Acknowledgement……………………………………………………………….1

CHAPTER I: THE PROBLEM AND ITS SETTINGS

Introduction...................................................................................................2
Statement of The Problem............................................................................3
Hypotheses...................................................................................................4
Significance of the Study...............................................................................5
Scope and Limitation of the Study.................................................................6
Conceptual Framework..................................................................................7
Definition of Key Terms..................................................................................8

CHAPTER II: REVIEW OF RELATED LEGAL BASES, LITERATURES AND STUDIES

Review of Related Legal Bases.......................................................................9
Review of Related Local Studies....................................................................10
Definition of Key Terms...................................................................................11
References......................................................................................................12
CHAPTER III: METHODOLOGY
Gantt Chart………………………………………………………………………….13

Materials and Procedure…………………………………………………………...14

Risk and Safety (Precautions)……………………………………………………..15

APPENDICES

Appendix A……………………………………………………………………………16

Appendix B………………………………………………………………………..….17

Appendix C…………………………………………………………………………...18

Biographical Data………………………………………………………………….…19
 ACKNOWLEDGEMENT
The researcher wishes to express her deepest gratitude and appreciation to caring
people who assist her and provide her with a proper assistance, unwavering support
and encouragement in order to make her research possible, as listed below. These are
the people who gladly contribute to the development of the study and the making of the
paper:

To my Very Loving Mother, Jenny V. Bataluna- for her patience and unyielding love
for the researcher, for providing appropriate financial support, for supplying the
necessary resources, and, of course, for her wonderful support and undying love for the
researcher.
To my Research Adviser, Mrs. Jane Crystal B. Bayas- For reviewing the researchers
work at any time and for assisting the researcher with her competent and smart
knowledge to make the study report more informative regardless her hectic schedule.
To our School Principal, Dr. Prudencio Animas- To encourage SSC students to
make research the foundation of academic success, as well as to provide a workspace,
facility, laboratory space, and other resources that assist the researcher in conducting
out the research study.
And To Our Almighty God- for giving me holistic, unwavering religious and moral
support, the strength that motivates me to pursue my research as a path to my success.

1
 CHAPTER I

THE PROBLEM AND

ITS SETTING
 INTRODUCTION

As time pass we age, with that our skin, becomes wrinkly, our bones become
fragile, and many more. The reason I want to lunch this study is that I want people to
maintain their beautiful and healthy skin that is cheap and trustworthy.

According to Bjarnadottir A. (2019), The tomato (Solanum lycopersicum) is a fruit

from the nightshade family native to South America. Despite botanically being a fruit, it’s
generally eaten and prepared like a vegetable. Tomatoes are the major dietary source
of the antioxidant lycopene, which has been linked to many health benefits, including
reduced risk of heart disease and cancer. They are also a great source of vitamin C,
potassium, folate, and vitamin K. Usually red when mature, tomatoes can also come in
a variety of colors, including yellow, orange, green, and purple. What’s more, many
subspecies of tomatoes exist with different shapes and flavors.

The researcher thought of conducting an experimental study using tomato

(Solanum lycopersicum) in order to produce a serum for the face. The people, and also
the researcher will gain more knowledge and information about this study. It can also
environment friendly and cheap product.

2
 STATEMENT OF THE PROBLEM

The main objective of this study is to make an alternative and effective treatment using
the Lycopene content in Tomato (Solanum lycopersium) seed extract as the main
ingredient in making an organic anti-wrinkle serum. This study also attempts to test the
effectiveness of Tomato seed extract as substitute to the other commercial product.
This study will answer the following questions:
1. Is Tomato seed extract possible to be used as the main ingredient in making an
organic anti-wrinkle serum?
2. Does the Tomato seed extract serum is effective in avoiding wrinkles in the
face?
3. To what amount of organic Tomato seed serum drop is most effective in avoiding
wrinkles in the face?

3
 HYPOTHESES
1. Is Tomato seed extract possible to be used as the main ingredient in making an
organic anti-wrinkle serum?

HO: It is not possible to use Tomato seed extract as the main ingredient in making
an organic anti-wrinkle serum.

HA: It is possible to use Tomato seed extract as the main ingredient in making an
organic anti-wrinkle serum.

2. Does the Tomato seed extract serum is effective in avoiding wrinkles in the face?

HO: The Tomato seed extract serum is not effective in avoiding wrinkles in the
face.

HA: The Tomato seed extract serum is effective in avoiding wrinkles in the face.

3. To what amount of organic Tomato seed serum drop is most effective in avoiding
wrinkles in the face?

HO: There is no amount of organic Tomato seed serum drop that is effective in
avoiding wrinkles in the face.

HA: There is an amount of organic Tomato seed serum drop that is effective in
avoiding wrinkles in the face.

4
 SIGNIFICANCE OF THE STUDY
The significance of the study is to make a cheaper and effective alternative medicine in
normalizing blood sugar. This study will contribute to the following:

To the Community: Since many people get wrinkles as they age and the products that
are in the store are a lot expensive the researcher thought of doing a study to make a
cheaper and effective alternative in avoiding wrinkles and still maintain the tightness of
the skin.

To the Future Researcher: This study will serve as their guide for further innovation and
more information about the Lycopene content of the Tomato seed.

To the Researcher Herself: This study will help the researcher to attain more knowledge
and skills for the improvement of the study, and explore more about the properties of the
Tomato Seed.

5
 SCOPE AND LIMITATIONS OF THE STUDY
This study will focus only on the Lycopene content in Tomato (Solanum lycopersium)
Seed extract as the main ingredient in making an organic anti-wrinkle serum. The
results will be used as an alternative in avoiding wrinkles. The researcher will gather all
materials needed at Blk 11 Lot 5 Phase 2 BellaVita Subdivision, Brgy Tapia, General
Trias City of Cavite.

6
 CONCEPTUAL FRAMEWORK

Lycopene Content in Tomato (Solanum lycopersium) Seed Extract as the Main

Ingredient in Making an Organic Anti-wrinkle Serum.
Applied Science

1. Is Tomato seed extract possible to be used as the main ingredient in making an

organic anti-wrinkle serum?
2. Does the Tomato seed extract serum is effective in avoiding wrinkles in the
face?
3. To what amount of organic Tomato seed serum drop is most effective in avoiding
wrinkles in the face?

INPUT PROCESS OUTPUT

-Collection of materials and
Gravity Filtration equipment
that to be used in creating
the experimental output.
-Collection of the Tomato
Seed (Solanum
lycopersicum) that will
undergo extraction.
Analyzation and Testing of
the Tomato (Solanum
lycopersicum) Seed Extract
as the main ingredient in
making an organic Anti-
wrinkle Serum.
The result of the output
shows effectiveness of
Tomato (Solanum
lycopersicum) Seed extract
as the Main Ingredient in
Making an Organic Anti-
wrinkle Serum.
The study manifest that is
possible to create a
homemade organic Anti-
wrinkle Serum out of Tomato
(Solanum lycopersicum)
Seed.

Figure 1. shows the effectiveness of the Lycopene content in Tomato (Solanum lycopersium) seed
extract as the main ingredient in making an organic anti-wrinkle serum.

7
 DEFINITION OF KEY TERMS
During the investigation, the researcher comes across a term that we are unfamiliar with
Hopefully, the glossary of terms and their definitions will provide comprehension to the
readers.
Serum- an amber-colored, protein-rich liquid that separates out when blood coagulates.

Organic- produced or involving production without the use of chemical fertilizers,

pesticides, or other artificial agents.

Anti-wrinkle- intended to reduce the appearance of wrinkles in the skin

Lycopene- a red carotenoid pigment present in tomatoes and many berries and fruits.

8
CHAPTER II
REVIEW OF RELATED LITERATURE
AND STUDIES
 REVIEW OF RELATED LEGAL BASES

As stated in section 5 of The 1897 Constitution Of The Republic Of The

Philippines – Article XIII Social Justice and Human Rights “The State shall recognize
the right of farmers, farmworkers, and landowners, as well as cooperatives, and other
independent farmers’ organizations to participate in the planning, organization, and
management of the program, and shall provide support to agriculture through
appropriate technology and research, and adequate financial, production, marketing,
and other support services.”

According to the Food and Drug Administration (FDA) (formerly the Bureau of
Food and Drugs) created under the Department of Health “All cosmetic establishments
shall first secure the LTO or authorization from FDA prior to engaging in the
manufacture, importation, exportation, sale, offering for sale, distribution, transfer,
promotion, advertisement and for sponsorship of any activity that involves cosmetics.
They must continuously comply with the existing requirements, regulations and
standards and under the supervision of a qualified person as required by the
regulations. The responsibility of ensuring the safety, quality, and when applicable, the
efficacy and/or purity of health products, shall rest upon all the establishments or
persons involved in the production, sale, handling, packing, transport, distribution,
trading and storage thereof.”

Republic act no. 3720 is an act to ensure the safety and purity of foods, drugs,
and cosmetics being made available to the public by creating the food and drug
administration which shall administer and enforce the laws pertaining thereto.

9
 REVIEW OF RELATED FOREIGN STUDIES

Lavecchia, Zuorro (2012) wrote “Big amounts of tomato peel and seed are going to waste,
and this waste is rich in lycopene with antioxidant and chemopreventive properties and
many more nutrients.”

Another study on the topic by (Biswas, 2019) maintains that there is a huge amount of
lycopene in tomatoes which is a source of antioxidant vitamin A, vitamin C, vitamin K,
potassium, and folate, wherein can avoid heart risk and cancer. Putting or rubbing
tomatoes every day on your skin can shrink pores and clean the skin, because of the rich
amount of lycopene and antioxidants making skin tight and keep away from wrinkles.

Pavey (2020) stated that lycopene can keep the natural firmness of the skin, avoiding
wrinkles and having young and healthy skin.

10
 DEFINITION OF KEY TERMS

During the investigation, the researcher comes across a term that we are unfamiliar with
Hopefully, the glossary of terms and their definitions will provide comprehension to the
readers.

Antioxidant- A substance that protects cells from the damage caused by free radicals.

Chemopreventive- The use of drugs, vitamins, or other agents to try to reduce the risk
of, or delay the development or recurrence of, cancer.

Lycopene- A red carotenoid pigment present in tomatoes and many berries and fruits.

Folate- A nutrient in the vitamin B complex that the body needs in small amounts to
function and stay healthy.

11
 REFERENCE

Zuorro, A., Lavecchia, R., Medici, F., & Piga, L. (2012). Enzyme-Assisted Production of

Tomato Seed Oil Enriched with Lycopene from Tomato Pomace. Food and Bioprocess

Technology, 6(12), 3499–3509. Retrieved from: https://doi.org/10.1007/s11947-012-

1003-6

Biswas, J. (2019, November 20). Benefits of Using Tomato For Face – The Urban

Guide. Urbancompany.com. Retrieved from:

https://www.urbancompany.com/blog/beauty/benefits-ofusing-tomato-for-face/

Goldfaden, G. (2021, June 6). Topical Lycopene Improves Skin Cellular Function.

Lifeextension.com; Life Extension Magazine®. Retrieved from:

https://www.lifeextension.com/magazine/2012/9/topical-lycopene-improves-skin-cellular-

function

THE 1987 CONSTITUTION OF THE REPUBLIC OF THE PHILIPPINES – ARTICLE

XIII | GOVPH. (2021). Official Gazette of the Republic of the Philippines. Retrieved from:

https://www.officialgazette.gov.ph/constitutions/the-1987-constitution-of-the-republic-of-

the-philippines/the-1987-constitution-of-the-republic-of-the-philippines-article-

xiii/#:~:text=The%20State%20shall%20afford%20full,of%20employment%20opportuniti
es%20for%20all.&text=They%20shall%20be%20entitled%20to,work%2C%20and%20a

%20living%20wage.

ChemLinked Team, & ChemLinked Team. (2020). Philippines Cosmetic Regulation |

ChemLinked. ChemLinked; chemlinked. Retrieved from:

https://cosmetic.chemlinked.com/cosmepedia/philippines-cosmetic-regulation

Republic Act No. 3720. (2021). Lawphil.net.

Retrieved from: https://lawphil.net/statutes/repacts/ra1963/ra_3720_1963.html

12
CHAPTER
III

METHODOLOGY
 GANTT CHART

Activities WEEK WEE WEE WEE WEE WEE WEE WEE

1 K2 K3 K4 K5 K6 K7 K8
(May (May (May (June (June (June (June (July
17-21) 24- 31- 7-11) 14- 28- 5-9)
28) June 18) 21- July
4) 25) 2)

1. Planning
the
experimen
t process.

2. Setting the
experimental
design.

3. Gathering
of information on
how to make the
desired product.

4. Preparatio
n of the
workplace.

5. Gathering
Equipment that is
to be used.

6. Collection
of the variable:
Tomato Seed
extract.

7. Producing
the desired
product.

8. Cleaning
the workspace.

9. Pre-testing
the product.

13
Research Materials and Procedure
INTRODUCTION ABOUT THE PROCEDURE
To be able to test the effectiveness and possibility of Tomato Seed as the main
variable in crafting the anti-wrinkle serum. Below the researcher will perform the
methodology. The procedure is subdivided into 4 parts which are the collection of the
materials, the process of making the tomato seed serum, making of the final product,
and the comparison, the researcher executes three set-ups containing different amount
of drop of the tomato seed extract anti-wrinkle serum. Safety measures shall be
observed in the experimentation proper.

A. GATHERING OF THE MATERIALS

In order, to perform this study, the researcher will gather all materials
needed such as and kitchen food scale gram for accurate measurement, utensils or
tools, jar, materials needed for gravity filtration, grapeseed oil, and bicarbonate sodium.

B. PROCESS OF MAKING THE TOMATO SEED SERUM

The researcher will get 1-kilo of Tomatoes from their Backyard and then
get the seed of the tomato. Put the seed in a jar next put 50ml of grapeseed oil after that
put ½ teaspoon of bicarbonated sodium or baking soda. Close the jar tightly and store it
in a cool dry place and shake every day for 1 week.

C. MAKING OF THE FINAL PRODUCT

Use gravity filtration in separating the extract of the seed and put it in a
jar. Get a container with hot water in it, and put the jar on it to furthermore separate the
desired product from the waste product. After getting the desired product which is the oil
extract of the tomato seed put it in the serum container.

D. TRIALS / SET-UP
For the trial proper, the researcher prepares all the ingredients that needs
to be added in each set-up and Tomato Seed Serum. For the 1st set-up 3 drops of the
serum is going to be used, for the 2nd set-up 4 drops of serum is going to be used, and
for the 3rd set-up 5 drop of the serum is going to be used. The amount of drops will
determine what amount of the Tomato Seed Anti-wrinkle Serum is more effective.
14
 RISK AND SAFETY (PRECAUTIONS)

The researcher was directed by the following risk and safety precautions
while carrying out the experimental output in order to conduct the study safely.

1. Have an adult to supervised

2. Make sure the workplace is clean and safe
3. Wear protective coats (lab gown), gloves, and a mask
4. Ask for assistance if you have no idea of what you are doing
5. Clean the workplace after the experiment

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 LIST OF
APPENDICES
 APPENDIX A: TABLE OF COMPARISON FOR THE MATERIALS AND
INGREDIENTS USE FOR TRIALS

AMOUNT / USAGE
MATERIALS AND
INGREDIENTS
SET-UP 1 SET-UP 2 SET-UP 3
Tomato 1kg 1kg 1kg

Grapeseed Oil 50ml 50ml 50ml

Baking Soda ½ Teaspoon ½ Teaspoon ½ Teaspoon

Erlenmeyer Flask 1 piece 1 piece 1 piece

Filter Paper 1 piece 1 piece 1 piece

Ring 1 piece 1 piece 1 piece

Short Stem 1 piece 1 piece 1 piece

Funnel

Serum Container 1 piece 1 piece 1 piece

Jar 1 piece 1 piece 1 piece

16
 APPENDIX B: TABLE OF COMPARISON FOR TOTAL COST MADE VS.
COMMERCIAL PRODUCTS

OWN COMMERCIAL PRODUCTS

PRODUCTS
MATERIALS
AND PRODUCT PRODUCT PRODUCT PRODUCT
INGREDIENTS A B C D

Tomato NO COST

Grapeseed Oil ₱80

Baking Soda NO COST

Fresh Tomato Skin Potions Tomato
Erlenmeyer NO COST Booster Tomato Serum Serum
Flask Serum Skin Potions (Organic-
(₱279.00) (₱266.00) Crueltry
Filter Paper NO COST free) by
KHM
Ring NO COST (₱212.00)
(₱212.00
Short Stem NO COST
Funnel

Serum ₱36
Container

Jar NO COST

TOTAL COST: ₱116.00 ₱279.00 ₱266.00 ₱212.00

17
APPENDIX C: RESEARCH MATERIALS AND EQUIPMENTS

18
 BIOGRAPHICAL DATA

The researcher, Dazzle Jean B. Alcordo, 13 years old was born on the 5 th
day of October 2007 in General Trias Maternity Hospital at Tejero, General Trias City,
Cavite and currently living at Brgy. Tapia, General Trias City, Cavite. She is the child of
45 years old, Jenny V. Bataluna (Real Estate Seller) and 42 years old Anthony Ross B.
Alcordo, an OFW Trailer Driver. She has an older Step-Brother that is 25 yearsold
“Shyrwin John Bataluna” an Assistance Admin in BellaVita HOA, and also a
Businessman.

The researcher completed her 6 years elementary journey from 2013-

2019 at General Artemio Ricarte Memorial School, Brgy.Bagumbayan, General Trias
City, Cavite. Currently she is studying in Governor Ferrer Memorial National High
School –MAIN, Brgy. Pinagtipunan, General Trias City, Cavite as a Grade 8-Anthurium
SSC student. She is a consistent honor student starting from Grade 5 when the
researcher fully showed her potential.

The researcher has an interest in playing Valorant, Call of Duty, and

Mobile Legends. She likes to watch K-dramas, and a Kpop Stan. She describes herself
as a talkative person. Her hobbies are Drawing, Playing games and her dog named
Pandot, Dancing, and Reading. The researcher is determined to pursue her dream
being an Architect graduate in University of Santo Tomas in Manila.

19