Lazar Et. Al. - Mitigating Eggplant Processing Waste's Environmental Impact Through

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Trends in Food Science & Technology 147 (2024) 104414

Contents lists available at ScienceDirect

Trends in Food Science & Technology


journal homepage: www.elsevier.com/locate/tifs

Mitigating eggplant processing waste’s environmental impact through


functional food developing
Nina-Nicoleta Lazăr a, Gabriela Râpeanu b, *, Cătălina Iticescu a, c
a
“Dunărea de Jos” University of Galati, REXDAN Research Infrastructure, 98 George Coșbuc Street, 800385, Galați, Romania
b
“Dunărea de Jos” University of Galati, Faculty of Food Science and Engineering, 111 Domnească Street, 800008, Galati, Romania
c
“Dunărea de Jos” University of Galaţi, Faculty of Sciences and Environment, 111 Domnească Street, 800008, Galati, Romania

A R T I C L E I N F O A B S T R A C T

Handling Editor: Dr AR Jambrak Background: Food wastes and by-products gained global focus due to rising amounts prompting pollution con­
cerns, regardless of the country’s development. Circular economy offers a sustainable solution, aiming to pre­
Keywords: vent, reuse, and recover resources for creating new, health-beneficial products.
Anthocyanins Scope and approach: The paper highlights the underexplored potential of anthocyanins found in the eggplant peel,
Extraction
a topic that has received less attention compared to the pulp. Considering the economic and technological
Encapsulation
relevance of anthocyanins, this paper reviews the ways through which these compounds can be valorized and
Functional food
Solanum melongena emphasizes their potential for food industry applications and, implicitly, waste minimization. The review
compiles existing data related to eggplant peel as a source of anthocyanins and reviews various methods for their
extraction and encapsulation. Additionally, it touches on incorporating the capsules into diverse food matrices,
considering the challenges they impose and regulatory aspects.
Key findings and conclusions: Eggplant peel anthocyanins can effectively serve as a natural substitute for artificial
preservatives and colorants in the food industry. Through encapsulation, fortified food products with improved
attributes that could positively impact human health and aid in waste management can be delivered. Never­
theless, additional research is essential to explore more detailed their application and health-promoting benefits.

1. Introduction encouraged to substitute chemical ingredients with natural ones due to


growing client demands. These specifications apply to food products
Food waste constitutes the largest portion of waste deposited in that offer insufficient levels of nutrients essential for a healthy diet, but
landfills and is distinguished by its high moisture content and microbial also to those who use chemical colorants and preservatives (Galanakis,
instability, presenting challenges in its management, regardless of its 2021). Therefore, there is an obvious need for the development of in­
source (Nayak & Bhushan, 2019). Moreover, when these wastes gredients through the addition of natural extracts that present multiple
decompose in landfills, they emit gases that contribute to the green­ functionalities as natural alternatives to improve the sensory charac­
house effect. Consequently, there is a critical need to devise a strategy teristics, nutritional value, and technological functions, but also to
for reducing these losses (Du et al., 2018). The European Union has provide biological properties (Galanakis, 2021).
introduced several initiatives, such as the circular economy for the value A class of compounds that can help to fulfill the objectives mentioned
of goods, materials, and resources preservation within the economy for above are the anthocyanins from plants (Mohammed & Khan, 2022).
an extended duration and reducing the generation of waste. One strat­ They are recognized for the health benefits they provide. For example,
egy in this endeavor involves transforming industrial processing waste they possess the potential to neutralize free radicals through oxidation
into valuable by-products by incorporating them into high-value food or reduction processes (Mattioli et al., 2020). Through this mechanism,
products (Baiano, 2014) as presented in Fig. 1. they can prevent the denaturation of proteins, lipids, or even DNA by the
In the ever-evolving landscape of nutrition and functional foods, the free radicals, and thus the appearance of cancer, neurodegenerative,
quest for natural sources of bioactive compounds with health-promoting cardiovascular, or liver diseases (Gürbüz et al., 2018; Lobo et al., 2010).
properties has intensified. In addition, the food industry is also being The content of these chemicals in food, their bioaccessibility upon

* Corresponding author.
E-mail address: gabriela.rapeanu@ugal.ro (G. Râpeanu).

https://doi.org/10.1016/j.tifs.2024.104414
Received 11 December 2023; Received in revised form 26 February 2024; Accepted 1 March 2024
Available online 4 March 2024
0924-2244/© 2024 Elsevier Ltd. All rights reserved.
N.-N. Lazăr et al. Trends in Food Science & Technology 147 (2024) 104414

ingestion, their interaction with other molecules, and their degree of this purpose. Moreover, we explored the challenges, regulatory aspects,
polymerization, among other things, all affect their bioactivity (Eker and prospects that guide the path toward unlocking the full potential of
et al., 2019). Few studies have addressed the production of new foods this underexplored resource. Anthocyanins are potent antioxidants with
employing these chemicals, despite the enormous technological poten­ various health benefits, including anti-inflammatory and cardiovascular
tial (de Araújo et al., 2021). protective properties (Niño-Medina et al., 2017). By harnessing these
One potential source of anthocyanins, often overlooked but teeming compounds from eggplant peels, we can create functional foods with
with promise, is the eggplant peel. While the succulent pulpy flesh of the enhanced nutritional profiles. The extraction of anthocyanins and other
eggplant is a familiar food in kitchens worldwide, its vibrant and bioactive compounds from eggplant peels opens doors to the develop­
nutrient-rich outer layer - the peel - has remained largely underexplored. ment of novel functional ingredients. These ingredients can be incor­
Solanum melongena L. belongs to the Solanaceae family and represents a porated into a wide range of food products, including beverages, snacks,
crop with a high production yield (Agregán et al., 2021). In 2021, the sauces, and baked goods, to provide health-promoting properties and
worldwide production quantity of eggplant was 58,646,098.21 tonnes aesthetic appeal.
(FAOSTAT). A major source of carbohydrates, proteins, dietary fiber, There aren’t many publications that discuss eggplant peels in the
vitamins, minerals, and bioactive compounds, eggplants are particularly literature. Some studies address eggplant pulp as a source of phenolic
nutrient-dense vegetable (Agregán et al., 2021). They are low in calories acids, and only a few peel Karimi et al. (2021), for instance, review the
and high in minerals and fibers. Various studies have been published potential of eggplant peels to be used as a valuable source of bioactive
addressing the extraction of pectin since they are rich in it (Higuer­ chemicals in terms of extraction techniques and potential applications.
a-Coelho et al., 2023; Kazemi et al., 2019a; Kazemi et al., 2019b). The However, too few of these research addresses encapsulation; instead,
peel of the eggplant has drawn particular interest due to its high level of they all focus on extraction as a valorizing method. Thus, our review also
phenolic compounds (mostly anthocyanins), which have antioxidant addresses the microencapsulation as a valorizing method for the
effects (Guohua Cao et al., 1996). What makes eggplant peels a unique eggplant peel anthocyanins, and their addition to food products as a
and valuable source of anthocyanins is their relative abundance. Often functionality test.
discarded as culinary byproducts or agricultural waste, these peels
possess an untapped potential to contribute to a sustainable and 2. Types of anthocyanins in eggplant peels
health-conscious food industry. In an era where consumers seek natural
and clean-label ingredients, the pigments extracted from eggplant peels Plants create anthocyanins during development and reproduction
can replace synthetic food colorings and additives, contributing to cycles for a variety of reasons, including disease defense, UV protection,
cleaner and more sustainable food production (Muhamad et al., 2018). and responses to environmental stress (Khoo et al., 2017). Anthocyanins
In addition, the inclusion of eggplant peel extracts in food products may occur in hundreds of different varieties in nature (Karimi et al., 2021) as
increase nutrient density. These extracts provide essential vitamins, glycosides of pelargonidin, cyanidin, delphinidin, peonidin, petunidin,
minerals, and antioxidants, enhancing the overall nutritional value of and malvidin (Fang, 2015). However, the purple color of eggplant peel is
the end products and aligning with consumer demands for healthier mainly due to delphinidin glycosides (Chatterjee et al., 2013). Though,
options (Ramos et al., 2014, pp. 373–394).Considering the economic delphinidin derivatives are not the only anthocyanins in the eggplant
and technological relevance of anthocyanins, the objective of this paper peel, in the literature being identified also cyanidin, petunidin, and
was to summarize the ways through which these compounds from malvidin derivatives (Khoddami et al., 2013). The chemical structures of
eggplant peel can be valorized and to emphasize their potential for ap­ these compounds are presented in Fig. 2.
plications in the food industry. Eggplant peels constitute a significant The most widely used method for the separation and identification of
portion of the vegetable, and their frequent disposal contributes to food anthocyanins is liquid chromatography coupled with mass spectrometry
waste. By recognizing the potential of eggplant peels as a valuable (Khoddami et al., 2013). Chromatographic methods have the ability to
source of bioactive compounds, we can contribute to the reduction of separate all the components under analysis, along with any potential
food waste and promote sustainable agricultural practices. In this re­ derivatives or degradation products. This capability allows for the ac­
gard, the data and publications now available about eggplant peel and curate determination of analytes present at low concentrations, even in
its potential as a source of bioactive compounds have been compiled and the presence of numerous interfering and coeluting substances (Kalili &
summarized. The major approaches to valuing anthocyanins found in de Villiers, 2011; Stalikas, 2007). Broadly, two types of chromato­
eggplant peels and their uses in the food sector have been explored for graphic techniques were employed to separate and characterize the

Fig. 1. Conversion of eggplant peels into functional food ingredients.

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N.-N. Lazăr et al. Trends in Food Science & Technology 147 (2024) 104414

3. Biological activities of eggplant peel anthocyanins

Most of the anthocyanin’s functionalities are related to its antioxi­


dant power (Tena et al., 2020). Eggplant fruit peel rich in anthocyanins
shows particularly high superoxide scavenging activity (SOS). More­
over, anthocyanins are recognized also as reactive oxygen species (ROS)
and reactive nitrogen species (RNS) scavenging (Enaru et al., 2021).
Nasunin isolated from the peel of purple eggplant fruit was associated
with both suppression of hydroxyl radical formation and superoxide
scavenging activity (Akanitapichat et al., 2010).
In a previous work (Condurache Lazăr et al., 2021), we managed to
produce extracts with high antioxidant activity that corresponded to
conventional and UAE extraction methods using 70% ethanol. Thus,
values of 39.85 ± 3.19 mM TE/g DW for conventional extraction at
50 ◦ C and 34.31 ± 0.42 mM TE/g DW for UAE extraction at 25 ◦ C were
achieved.
Boulekbache-Makhlouf et al. (2013) reported the antioxidant activ­
ity of eggplant peel ranging between 58.81 ± 1.1% and 63 ± 0.48%
when they tested the solvent extraction combined with stirring for 40
min at room temperature. They used three solvents (70% methanol, 70%
ethanol, and 70% acetone) containing 0.2% formic acid. Comparatively
to the acetonic and ethanolic extracts, the methanolic one displayed the
strongest radical scavenging efficacy against DPPH and H2O2. Hosseini
et al. (2016) reported the eggplant peel’s capacity to scavenge DPPH
ranging from 24.09% to 71.68% using the conventional approach
varying the solvents (water, absolute ethanol, water/ethanol 1:1,
Fig. 2. The structures of the main anthocyanidins from eggplant water/ethanol/citric acid, water/ethanol/acetic, and water/­
peel (PubChem). ethanol/hydrochloric acid in a 50:48:2 ratio) for 60 min in the dark.
Following these results, the authors stated that the concentration of
anthocyanins from eggplant peels such as a high-performance liquid anthocyanins in eggplant peel extracts had a significant impact on their
chromatograph with a diode array detector (HPLC-DAD) and a capacity to scavenge free radicals. Horincar et al. (2019) stated that
high-performance liquid chromatograph coupled with a tandem mass methanolic extract produced by UAE seemed to have the highest ca­
spectrometer (HPLC-MS/MS), as detailed in Table 1. HPLC-DAD sepa­ pacity for antioxidant activity among the polyphenolic extracts (51.15
rates and identifies analytes using their proprieties to absorb light in the ± 0.57%), followed by the ethanol and acetone extracts (39.69 ± 0.63%
UV–Vis spectrum, while HPLC-MS separates and identifies analytes and 33.52 ± 0.58%). Chatterjee et al. (2013) reported that solvent
based on their molecular weight and fragmentation pattern (Chew et al., extraction inhibited the free radical in a range of 87.39–89.57%, while
2021). SC-CO2 was in the range of 59.28–91.16% when using the DPPH and
Typically, the literature emphasizes the use of gradient mobile FRAP methods.
phases when isolating and identifying the anthocyanins from eggplant Nevertheless, the antioxidant activity is not the only biological ac­
peels, as outlined in Table 1. These phases comprise a combination of tivity that eggplant peel exerts. Phenolic compounds were reported to
two or more reagents, commonly polar organic solvents such as meth­ inhibit in vitro the enzymes involved in the mechanism of metabolic
anol or acetonitrile along with a mild acid such as acetic or formic acid syndrome disease appearance (Kaur et al., 2021). Salivary glands pro­
(Mizzi et al., 2020). duce α-amylase that breaks down starch molecules and hydrolyzes them
Six glycosides of delphinidin were identified in the eggplant peel in into glucose or maltose by acting on the − 1,4 glycosidic linkages
the specialized literature, the major one identified by most of the re­ (Kaushal et al., 2022). Lipoxygenase is involved in several
searchers being delphinidin 3-O-rutinoside as shown in Table 1 (Azuma inflammation-related disorders by catalyzing the formation of hydro­
et al., 2008; Braga et al., 2016; Horincar et al., 2019; Philippi et al., peroxides from polyunsaturated fatty acids (Snodgrass & Brüne, 2019).
2016). The same glucoside of delphinidin was also the main form of Triglycerides are broken down into free fatty acids and glycerol by
anthocyanin discovered employing standards in earlier research that we lipase, an enzyme that catalyzes the hydrolysis of ester bonds in tri­
carried out (Condurache (Lazăr) et al., 2021; Condurache Lazăr et al., glycerides. An indication of pancreatitis is a high level of lipase in the
2021). Moreover, glycosides of cyanidin, malvidin, and petunidin were serum (Pirahanchi et al., 2022). By blocking the activity of these en­
also identified by various authors (Table 1). zymes, phenolics may aid in lowering hyperglycemia, obesity, over­
The differences between the types and concentrations of anthocya­ weight, and inflammation (Kaur et al., 2021). Kwon et al. (2008)
nins found in the eggplant peel are given by the plant species, cultivar, demonstrated that eggplant peel extracts have moderate to high
developmental stage, or agronomic conditions in general, but also by the α-amylase in vitro inhibition capacity. Moderate in vitro inhibition ca­
environmental factors (Luthria et al., 2010; Mekapogu et al., 2020; Zaro pacity of an eggplant peel extract against lipoxygenase and lipase,
et al., 2014). For instance, the highest concentrations of anthocyanin among α-amylase we were also able to obtain in previous work (Con­
occur during the unripe eggplant stage, decreasing as the fruit achieves durache Lazăr et al., 2021). Given all this evidence, it can be assumed
physiological maturity (Lo Scalzo et al., 2021). Kumari et al. (2022) that anthocyanins from eggplant peels, in the proper dose, may be
demonstrated as well that the concentrations of biologically active engaged in lowering the development of various disorders. However,
compounds in eggplant are strongly influenced by seasonal variation. more studies are required, especially in vivo studies, to support the in
Also, high temperatures inhibit anthocyanin biosynthesis while low vitro ones.
temperatures promote it both with and without light (Fang, 2015).
4. Extraction of anthocyanins from eggplant peels

Over time, different green, efficient, and economical methods for

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N.-N. Lazăr et al. Trends in Food Science & Technology 147 (2024) 104414

Table 1
Anthocyanin glycosides found in eggplant peel using HPLC techniques.
Anthocyanins in eggplant peel Chromatographic analysis Analysis parameters Reference

Delphinidin 3-O-rutinoside HPLC-DAD Synergi 4u Fusion-RP 80A (150 × 4.6 mm, 4 μm) column; mobile phases: Condurache Lazăr
520 and 280 nm 10% formic acid (FA) and 100% methanol (MeOH) under gradient et al. (2021)
conditions: 0–20 min, 9–35% (FA); 20–30 min, 35% (FA); 30–40 min, Condurache
35–50% (FA); and 40–55 min, 50–90% (FA) (Lazăr) et al.
(2021)
Horincar et al.
(2019)
LC-DAD-MS employing electrospray Endcapped Superspher RP-18 (125 × 2 mm, 4 μm) column; mobile phases Philippi et al.
ionization (ESI) in positive ion mode 1.5% acetic acid (AA) and MeOH with an elution program as follows: 0–2 (2016)
min, 0 % MeOH; 2–52 min, 100 % MeOH; and 60 min, 100 % MeOH.
HPLC-DAD TSKgel ODS-80Ts (4.6 mm × 250 mm) column; mobile phases acetonitrile Azuma et al.
240–600 nm in 10% (2008)
FA under the linear gradient of 0–40% mobile phases for 35 min
RP-HPLC-DAD Inertsil ODS-3 (250 × 6 mm i.d.) column; mobile phases AA 5% in water Braga et al.
and AA 5% in MeOH with the following (2016)
gradient: 98% AA for 20 min, decreased to 50% AA for 15 min,
kept at 50% AA for 10 min, and returned to the initial
conditions in 10 min
Delphinidin 3-O-glucoside HPLC-DAD mobile phases 10% FA and 100% MeOH Condurache Lazăr
et al. (2021)
Condurache
(Lazăr) et al.
(2021)
Horincar et al.
(2019)
HPLC-MS/MS employing electrospray Luna 3μ C18 100 Å (50 × 2.00 mm) column; mobile phases water and 0.5 Mauro et al.
ionization (ESI) in positive ion mode; m/z % trifluoroacetic acid (TFA) and MeOH (2020)
611, m/z 773, and m/z 920 ions using a gradient rate of 0–10 min 80 % TFA and 20 % MeOH; 10–12 min
100 % MeOH; and 12–14 min 80 % TFA and 20 % MeOH.
HPLC-DAD mobile phases acetonitrile in 10% Azuma et al.
FA (2008)
Delphinidin 3-O-rutinoside-5- HPLC-DAD mobile phases 10% FA and 100% MeOH Condurache Lazăr
glucoside et al. (2021)
Condurache
(Lazăr) et al.
(2021)
Horincar et al.
(2019)
HPLC-MS/MS mobile phases water and 0.5 % TFA and MeOH Mauro et al.
(2020)
HPLC-DAD Prontosil C18AQ (4.6 × 250 mm, 5 μm) column; mobile phases water–AA Ferarsa et al.
543 nm (93:7, v/v) and water–acetonitrile–MeOH–AA (47:23:23:7, v/v) (2018)
Delphinidin 3-O-rutinoside-5- HPLC-MS/MS mobile phases water and 0.5 % TFA and MeOH Mauro et al.
galactoside (2020)
Delphinidin-3-(p- HPLC-MS/MS mobile phases water and 0.5 % TFA and MeOH Mauro et al.
coumaroylrutinoside)-5- (2020)
glucoside (nasunin) HPLC-DAD mobile phases acetonitrile in 10% Azuma et al.
FA (2008)
RP-HPLC-DAD mobile phases acetic acid 5% in water Braga et al.
and AA 5% in MeOH (2016)
Delphinidin 3- HPLC-DAD mobile phases acetonitrile in 10% Azuma et al.
caffeoylrutinoside-5-glucoside FA (2008)
Cyanidin 3-O-rutinoside HPLC-DAD mobile phases 10% FA and 100% MeOH Condurache Lazăr
et al. (2021)
Condurache
(Lazăr) et al.
(2021)
Horincar et al.
(2019)
HPLC-MS/MS mobile phases water and 0.5% TFA and MeOH Mauro et al.
(2020)
Malvidin 3-O-rutinoside-5- HPLC-DAD mobile phases MeOH 100% and 10% FA Horincar et al.
glucoside (2019)
mobile phases water–acetic acid (93:7, v/v) and Ferarsa et al.
water–acetonitrile–MeOH–AA (47:23:23:7, v/v) (2018)
Petunidin 3-O-rutinoside HPLC-DAD mobile phases 10% FA and 100% MeOH Condurache Lazăr
et al. (2021)
Condurache
(Lazăr) et al.
(2021)
Horincar et al.
(2019)
mobile phases water–AA (93:7, v/v) and water–acetonitrile–MeOH–AA Ferarsa et al.
(47:23:23:7, v/v) (2018)
(continued on next page)

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Table 1 (continued )
Anthocyanins in eggplant peel Chromatographic analysis Analysis parameters Reference

Petunidin 3-(p- HPLC-DAD mobile phases acetonitrile in 10% Azuma et al.


coumaroylrutinoside)-5- FA (2008)
glucoside

HPLC-DAD - High-Performance Liquid Chromatography coupled with Diode Array Detector; RP-HPLC-DAD - Ion-pair Reversed Phase-High Performance Liquid
Chromatography coupled with Diode Array Detector; HPLC-MS/MS - High-Performance Liquid Chromatography coupled with a tandem Mass Spectrometer.

anthocyanins’ recovery from different plant sources have been sought addition of an acid in the extraction solvent facilitates the cell mem­
(Mariacaterina et al., 2022). These methods impart the techniques in brane’s rupture while stabilizing the anthocyanins since they are
conventional and modern Putnik et al. (2019). Among the conventional pH-dependent and have high stability in acidic environments (Hasbay &
ones, maceration, Soxhlet, and hydrodistillation are the most known. Galanakis, 2018).
Among the modern methods, ultrasound-assisted (UAE), Although conventional methods are efficient, they are time and
enzyme-assisted (EAE), microwave-assisted, pulsed-electric field, and energy-consuming, with modern ones being preferred. Horincar et al.
supercritical fluid extraction were highlighted (Roselló-Soto et al., (2019) employed ultrasound-assisted extraction with 70% methanol,
2016). Various studies have been published in the specialized literature ethanol, or acetone, each solvent prepared with distilled water, to obtain
regarding the extraction of biologically active compounds from eggplant eggplant peel extracts. Three successive extractions were carried out
peels, as presented in Table 2. while the samples were subjected to ultrasound treatment at 40 kHz and
With the use of the maceration approach, Kumari and Dhatt (2018) 40 ◦ C for 30 min. When compared to acetone, the authors found that
extracted the bioactives from the peel of 50 eggplant genotypes by methanol and ethanol produced higher concentrations of bioactive
resting it in 1% HCl acidified methanol overnight at 4 ◦ C, as shown in chemicals from eggplant peel. For methanol and ethanol extracts, TAC
Table 2. This method recovered a total anthocyanin content (TAC) in the showed a 0.74 ± 0.05 and 0.58 ± 0.03 mg DGE/g DW concentration,
range of 0.04–113.93 mg/100g peel. However, anthocyanins were not respectively. The acetone extract yielded a value of 0.36 ± 0.00 mg
detected in certain varieties. Jung et al. (2011) also employed the DGE/g DW. Dranca and Oroian (2016) varied methanol, ethanol, and
maceration process for 24 h at room temperature in the dark using 1% 2-propanol as extraction solvents. The sample was processed in an ul­
HCl acidified methanol. The eggplant peel extract showed a high trasonic bath at various temperatures (50, 60, and 70 ◦ C), sonication
anthocyanin content of 138.05 mg/100g. Since maceration is a frequencies (0, 25, and 45 kHz), and extraction times (10, 20, 30, 40, 50,
time-consuming method, Boulekbache-Makhlouf et al. (2013) tested the and 60 min). Following each treatment, the extract was filtered and
solvent extraction combined with stirring for 40 min at room tempera­ cooled to 20 ◦ C to be analyzed. The extraction of 54.4% methanol at 37
ture. They used three solvents (70% methanol, 70% ethanol, and 70% kHz, 55.1 ◦ C for 44.85 min produced the maximum TAC recovery of
acetone) containing 0.2% formic acid. After the methanolic extraction, 2410.71 mg C3G/kg. After achieving a TAC maximum yield of 5.88 ±
the authors reported a maximum anthocyanin concentration of 82.83 ± 0.39 mg/g, Liao et al. (2022) claimed that multi-frequency ultrasonic
1.07 mg DGE/100 g DP. While ethanol recovered 62.92 ± 0.15 mg extraction was an effective method to enhance the extraction of bioac­
DGE/100 g DP, acetone only recovered 51.56 ± 4.87 mg DGE/100g DP. tive components from eggplant peels.
Hosseini et al. (2016) used a variety of solvents and solvent mixtures Researchers also tested other modern extraction techniques to
(water, absolute ethanol, water/ethanol (1:1), water/ethanol/citric maximize the bioactive compounds extracted from eggplant peels. In
acid, water/ethanol/acetic, and water/ethanol/hydrochloric acid in a order to compare the solvent and supercritical carbon dioxide (SC–CO2)
50:48:2 ratio) for 60 min in the dark to extract anthocyanins from the extraction methods, Chatterjee et al. (2013) varied the parameters. To
peel of eggplant. Water extracts had the lowest anthocyanin concen­ achieve the best yield of extraction from the peels, different tempera­
tration of 5.61 mg/100g FW, while citric acid extract had the highest tures (23, 40, 60, and 80 ◦ C) and rotary speeds (100, 150, and 180 rpm)
anthocyanin content of 51.40 mg/100g FW. It is already known that the were used when the solvent extraction was employed. For the SC-CO2

Table 2
Extraction methods and parameters of anthocyanins from eggplant peels.
Plant matrix Extraction method Solvent Extraction parameters Anthocyanins’ maximum References
extraction yield

Conventional approaches
Eggplant Maceration Methanol and 1 % HCl 4 ◦ C, overnight 113.93 mg/100g peel Kumari and Dhatt (2018)
peels Methanol and 1 % HCl Room temperature, 24 h 138.05 mg/100 g peel (Jung et al., 2011)
Solvent extraction combined 70 % methanol and 0.2 % Room temperature, 40 min 82.83 ± 1.07 mg DGE/100 g Boulekbache-Makhlouf et al.
with stirring formic acid DP (2013)
Static solvent extraction Water/ethanol/citric acid in Room temperature, dark, 60 51.40 mg/100 g FW Hosseini et al. (2016)
a 50:48:2 ratio min
Solvent extraction in a rotary water-ethanol (1:1) and 10% 60 ◦ C 19.75 ± 0.08 g/kg DP Chatterjee et al. (2013)
shaker citric acid
Solvent extraction combined 96 % ethanol acidified with 1 50 ◦ C, 30 min 1.13 ± 0.10 mg D3G/DW Condurache Lazăr et al. (2021)
with stirring N HCl
Modern approaches
Eggplant UAE 70 % methanol 40 kHz, 40 ◦ C, 30 min 0.74 ± 0.05 mg D3G/g dw Horincar et al. (2019)
peels 54.4% methanol 37 kHz, 55.1 ◦ C, 44.85 min 2410.71 mg C3G/kg Dranca and Oroian (2016)
50 % ethanol 25 + 40 + 70 kHz, 45 ◦ C, 35 5.88 ± 0.39 mg/g Liao et al. (2022)
min
96 % ethanol acidified with 1 40 kHz, 30 min, 25 ◦ C 1.04 ± 0.10 mg D3G/g DW Condurache Lazăr et al. (2021)
N HCl
SC-CO2 CO2 60 C, 10 MPa, 1.5 h, 0.2 L

17.04 g/kg of dry peel weight Chatterjee et al. (2013)
min-1 of CO2
EAE Cellulase 37.32 ◦ C, 5% enzyme, 1 h 578.66 mg C3G/L (Amulya & ul Islam, 2023)

D3G – delphinidin 3-O-glucoside; C3G – cyanidin 3-O-glucoside; FW – fresh weight; DP – dried powder; DGE - delphinidin-3-glucoside equivalent.

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extraction, the peels were placed into the extraction vessel after there are options such as encapsulation. This method expands the
pre-treating them for 1 min in a 10% citric acid solution. Variable shelf-life of active compounds while reducing nutritional and sensory
extraction pressure (10, 12, 15 MPa), temperature (60, 80 ◦ C), time, and degradation (Fang & Bhandari, 2010).
2 L/min flow rate of CO2 were used to optimize the extraction of The optimization of encapsulation method for proper incorporation
anthocyanin from eggplant peels. TAC and the yield of the of bioactive compounds depends on the characteristics of the bioactive
solvent-extracted compounds have been seen to increase with higher molecule, the encapsulating material, the desired final product, and
extraction temperatures from 23 to 60 ◦ C. Both, the yield and TAC other factors (particle size, surface morphology, storage stability, etc.)
decreased once the temperature was raised to 80 ◦ C. The previously (Zabot et al., 2022). To protect the bioactive compounds against
mentioned mixture, heated to 60 ◦ C, produced the best yield of color degradation in along the processing chain and in vivo, the produced
with a TAC of 19.75 g/kg of dry peel weight. With an increase in tem­ capsules must contain a significant concentration of bioactive com­
perature, the yield and TAC of the SC-CO2 extracted dropped. It was pounds and be easily added in food matrices (Mehta et al., 2022). The
discovered that the retrograde phenomena of supercritical extraction time, expense, and steps necessary to obtain the capsules, as well as
occur at considerably higher pressure regimes (12–15 MPa). Therefore, production volume, market demands, and existing legislation, are other
it can be concluded that 60 ◦ C and 10 MPa were the ideal SC-CO2 con­ factors to consider (Celli et al., 2015).
ditions for extracting colors from eggplant peels with a high anthocyanin In recent years, the food industry has employed this technique
concentration (17.04 g/kg of dry peel weight). TACs of 4.25 ± 0.02 g/kg regularly to increase the nutritional content of food products (Ye et al.,
dried peel to 19.75 ± 0.08 g/kg dried peel were achieved using solvent 2018). This kind of substance has several benefits, including keeping the
extraction, whereas SC-CO2 values ranged from 2.95 ± 0.05 g/kg dried product stable for extended periods and lowering storage and shipping
peel to 17.04 ± 0.06 g/kg dried peel (Chatterjee et al., 2013). expenses (Celli et al., 2015).
Amulya and ul Islam (2023) compared the conventional extraction In the specialized literature, few published studies have as a theme
method with the enzyme-assisted one, optimizing the latter in the end. the encapsulation of biologically active compounds from eggplant peels.
They observed that cellulase facilitated the rupture of eggplant peel cells In 2013, Chatterjee et al. (2013) encapsulated eggplant peel extracts in
resulting in improved extraction yields and reducing the extraction time calcium alginate. The authors observed that an extract concentration
compared to the conventional technique. Thus, the maximum yield of increase over 15 g/L led to significant color loss, while decreasing the
phenolics from eggplant peel extracted with cellulase was 578.665 mg alginate concentration under 25 g/L led to unstable beads, incapable of
C3G/L for TAC after 1 h of extraction using 5% enzyme concentration retaining 10 g/L of extract. In addition, they reported that the beads
(Amulya & ul Islam 2023). with 10 g/L extract showed insignificant bioactive compound contents
In order to compare the total anthocyanins recovered using con­ and antioxidant activity. In the 25–45 g/L and 10–15 g/L ranges of so­
ventional and ultrasound-assisted approaches, we performed both in dium alginate and extract concentrations, they obtained 51–72%
previous work (Condurache Lazăr et al., 2021). For each method, encapsulation efficiencies of the bioactive compounds, the latter
different solvent concentrations (70 and 96% ethanol), times, and increasing in general with the concentration of wall material. However,
temperatures were used. For the conventional method based on stirring the highest encapsulation efficiency of 72.79% was observed for the
for 30–120 min, 70 and 96% ethanol acidified with 1 N HCl at 25 and beads prepared with 45.00 g/L sodium alginate and 12.50 g/L eggplant
50 ◦ C. For the ultrasounds-assisted extraction, the same ethanol con­ peel extract. For this encapsulation efficiency corresponds to 0.44 ±
centrations acidified with HCl were used at 40 kHz for 25–45 min at 25 0.02g D3G/kg of beads. The same parameters also led to antioxidant
and 50 ◦ C. Values between 0.75 ± 0.07 mg D3G/g DW and 1.13 ± 0.10 activity with an IC50 of 4.60 ± 1.33 μg/mL. A storage stability test in the
mg D3G/g DW were achieved for both techniques. The highest TAC in dark at 4 ◦ C for 60 days revealed high protection of the sodium alginate
the conventional extraction case was obtained at 50 ◦ C (1.13 ± 0.10 mg matrix on the anthocyanins from the eggplant peel, 16 times higher than
D3G/g DW). The extraction at 25 ◦ C yielded the highest TAC for the UAE the stability of the anthocyanins from the unencapsulated extract
(1.04 ± 0.10 mg D3G/g DW). Both methods presented a high anthocy­ (Chatterjee et al., 2013).
anin content at 96 % ethanol and 30 min. Although the TAC of the two Sarabandi et al. (2019) encapsulated the eggplant peel extract in
extracts were not significantly different, at the same ethanol concen­ maltodextrin, gum Arabic, and combinations of them (1:1) through
tration, using UAE has the advantage of lowering the temperature, spray drying. The temperature of the inlet air varied from 140 to 170 ◦ C
allowing for energy savings and minimizing the risk of phenolic during the drying process. The antioxidant activity values ranged from
degradation. 55.48 to 73.38%. The authors reported that both the wall material and
In general, the extraction of anthocyanins from eggplant peel with inlet air temperature influenced the antioxidant activity. Thus, malto­
methanol was the most efficient and used method. However, extraction dextrin entrapped high contents of compounds that provided the highest
with ethanol is preferable to methanol due to its lower toxicity if the antioxidant activity. However, a partial or complete substitution of
extracts aim to be added to food products. Although conventional maltodextrin with gum Arabic led to an antioxidant activity decrease.
extraction methods are efficient, they are nevertheless very time- and Horincar et al. (2020a) reported a 94.31 ± 3.32% efficiency of the
resource-consuming. Thus, ultrasound-assisted extraction is preferable. encapsulation of anthocyanins from eggplant peel in whey proteins and
However, other modern extraction methods should not be neglected acacia gum through freeze-drying. The powder presented a TAC of 0.837
either. ± 0.086 mg D3GE/g DW and antioxidant activity of 45.522 ± 6.789
mM TE/g DW. Barretto et al. (2020) encapsulated eggplant peel extract
5. Encapsulation of anthocyanins from eggplant peels in various concentrations of gum Arabic using the spray drying tech­
nique. The anthocyanins content ranged between 5.81 and 42.61
Despite all their benefits and advantages, due to the limited bio­ mg/100g peel depending on the inlet air temperature into the drying
accessibility and bioavailability of anthocyanins, the human body ab­ process, which ranged between 130 and 180 ◦ C and wall material con­
sorbs only 2% of the anthocyanins ingested dosage (Calderaro et al., centration, which ranged between 10 and 20%. However, the highest
2020). Additionally, they easily oxidize and suffer molecular modifica­ TAC was achieved at 150 ◦ C with 15% gum Arabic. They also reported
tions when exposed to excessive temperature, oxygen, and light condi­ an antioxidant activity of 92.25% inhibition.
tions during food processing and storage (Mattioli et al., 2020). Martini In 2019, our research group (Condurache et al., 2019) published a
et al. (2021) demonstrated that ordinary cooking methods degrade the study in which we encapsulated the eggplant peel extract in various
phenolics from eggplant, but on the other way help their release from combinations of carboxymethyl cellulose, pectin, whey proteins, and
the matrix during digestion. Thus, to enhance their bioavailability and whey peptides through freeze-drying. Four variants of microcapsules
bioaccessibility and protect them from degrading environmental factors, were prepared, having encapsulation efficiencies between 69.90 ±

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1.90% and 77.60 ± 1.92%. In addition, TAC contents ranging between color, flavor, and vitamins of food from potential damage caused by
0.52 and 0.89 mg D3G/g DW and antioxidant activities ranging between factors like light, air, and temperature. Thanks to their wide spectrum of
55.43 ± 7.17 and 94.71 ± 2.8 mM Trolox/g DW were achieved. The vibrant colors, they find applications in decorative uses, such as
encapsulation efficiency increased with the concentration of carbox­ enhancing cake icing. Furthermore, they can effectively conceal
ymethyl cellulose in the matrix. However, a slight instability of com­ inherent color variations, thereby enhancing the overall taste (Dey &
pounds during storage after 28 days at 25 ◦ C was noticed. In this case, Nagababu, 2022).
also, the powder variant with the highest carboxymethyl cellulose The industrial application of anthocyanins as natural pigments is
concentration protected better the anthocyanins from the environ­ constrained by their instability to the conditions during processing and
mental conditions during storage. In another previous study, our storage (Gençdağ et al., 2022), although they offer an alternative to
research team (Condurache (Lazăr) et al., 2021) encapsulated eggplant synthetic additives. They are also extremely susceptible to digestive
peel bioactive compounds in stabilized hydrogels through freeze-drying. system conditions at the same time (Verediano et al., 2021). However,
Two powders were produced in which the concentrations between several researchers have attempted to add non-encapsulated extracts
carboxymethyl cellulose, pectin, and whey proteins were varied. As in from eggplant peels to various food products, as shown in Table 3. For
the 2019 study, also in this case it was observed that the increase in the instance, Horincar et al. (2020b) found that anthocyanin levels in a beer
concentration of carboxymethyl cellulose from the matrix leads to an enhanced with eggplant peel extract significantly decreased with time.
increase in the encapsulation efficiency and, implicitly, to the retention In addition, Zhang et al. (2020) reported anthocyanin degradation from
of the highest concentrations of biologically active compounds. Thus, cookies enriched with eggplant peel extract after their thermal treat­
the highest encapsulation efficiency of 96.44 ± 3.43% was achieved for ment. Moreover, according to Aboelhaggag et al. (2023), eggplant peel
the powder with higher polysaccharides than protein content compared extracts may still improve the quality, taste, and acceptability of foods
to the powder with similar content of polysaccharides and proteins like sorbets compared to chemical dyes.
(64.67 ± 0.67%). In addition, they reported TAC contents ranging be­ By enhancing or creating new functions, the usage of anthocyanins in
tween 50.41 ± 2.13 and 94.94 ± 7.94 mg D3G/g DW. The antioxidant a variety of products results in functional foods that give customers extra
activity of the powders has values between 36.60 ± 0.83 and 41.96 ± health benefits. That is why some researchers have tried to incorporate
0.28 mM TE/g DW. microencapsulated anthocyanin extracts from eggplant peels into
A more recent study published by Sharma et al. (2023) deals with the different food products.
subject of eggplant peel bioactive compounds encapsulation by emul­ Horincar et al. (2020a) enriched a pastry cream with 5, and 10%
sification in various concentrations of soybean oil and emulsifier. They encapsulated eggplant peel extract. The encapsulation matrix consisted
reported an antioxidant activity starting from 0.81 ± 0.01 μM TE/mL to of 2.5% WPI and 1% acacia gum, freeze-drying being the technique used
1.03 ± 0.01 μM TE/mL. After 30 days of storage at 25 ◦ C, the antioxi­ for the stabilization of capsules. The amount of microparticles boosted
dant activity increased significantly. the antioxidant activity of the pastry cream samples, and the encapsu­
Regardless of the method and materials used, encapsulation led to lation matrix ensured good stability of the biologically active com­
increased stability of anthocyanins from eggplant peels. Thus, the pub­ pounds in the food matrix. However, the authors revealed significant
lished articles demonstrated that encapsulation could be a technique decreases in the anthocyanin content of the pastry cream enriched with
that promises to remove at least some of the limitations of this type of 5% microparticles from eggplant peel extract and high stability in cream
compound in terms of valorization. In addition, encapsulation may be a with 10%. The authors appreciated based on the sensory evaluation that
beneficial tool in customizing solutions to preserve and utilize phenolics encapsulated eggplant peel extract improved the product’s color.
since they are unstable when exposed to environmental and industrial Additionally, all pastry creams displayed rheological behavior specific
process conditions (Comunian et al., 2021). to a gel structure and had no impact on the samples’ adhesion, cohesion,
springiness, or chewiness.
6. Application of anthocyanins in the food industry Barretto et al. (2020) encapsulated anthocyanins from eggplant peel
in gum Arabic by spray drying and used the produced microparticles to
The visual element is a significant factor in how contemporary enrich yogurt. They reported a reduction in the concentration of an­
consumers choose food products, with color being a crucial component thocyanins in yogurt enriched with microencapsulated powder from
in both food and beverages. Phenolic compounds are suitable natural eggplant peel extract after 30 days of storage. However, microencap­
dyes for the bakery, beverage, confectionery, dairy, ice cream, and meat sulation protected the compounds. Sarabandi et al. (2019) fortified
industries. They can also be successfully utilized in cosmetics, pharma­ gummy candies with microencapsulated eggplant peel extract, and the
ceutics, or pet food (Dey & Nagababu, 2022). In the food industry, these sensory qualities of the final products were studied. They claimed that
substances were linked to the enhancement of naturally occurring the 1.5% powder addition enhanced the product’s color characteristics
colors. Additionally, they were noted for their ability to safeguard the and general acceptance.

Table 3
Food products enriched with eggplant peel bioactive compounds.
Source Encapsulation Encapsulation matrix Food Characteristics References
method matrix

Eggplant peel – – Lager beer increased phenolic content; good sensory characteristics; good stability Horincar et al.
extract without artificial preservatives; low anthocyanins stability (2020b)
– – Cookies low stability of anthocyanins after thermal treatment Zhang et al.
(2020)
– – Sorbet improved product quality, yellowness, taste and acceptability Aboelhaggag et al.
(2023)
freeze-drying Whey protein isolate, Pastry Increased concentration of bioactive compounds and antioxidant activity; high Horincar et al.
acacia gum cream stability of bioactive compounds in the food matrix during storage; improved (2020a)
color properties of the product
spray-drying Gum Arabic Yogurt high stability of encapsulated anthocyanins in the food matrix during storage Barretto et al.
compared to the non-encapsulated ones (2020)
spray-drying Maltodextrin, gum Gummy improved color properties and overall acceptability of the product Sarabandi et al.
Arabic candies (2019)

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In addition to food products with added value, anthocyanin extracts 8. Challenges and future research Directions
from eggplant were also used by researchers to create active packaging
since these protect food from environmental, physical, and microbial Working with eggplant peel anthocyanins, while promising, presents
contamination, also extending the shelf-life. Wang et al. (2023) suc­ several challenges that need to be addressed to maximize their utiliza­
cessfully developed a novel active food film packaging based on chito­ tion in food products and other applications. Anthocyanin extraction
san, chitin, and eggplant peel extract for monitoring pork meat spoilage. from eggplant peels can be inefficient due to their complex cell wall
They observed that due to the large amounts of anthocyanins and structures and matrix (Kaderides et al., 2019). Developing optimized
polyphenols from the eggplant peel extract, the obtained films showed extraction methods to achieve high yields while preserving anthocyanin
high antioxidant and antimicrobial capacities. stability is a challenge. Moreover, anthocyanins are sensitive to factors
Eggplant peel can be a valuable source of natural food dyes and like pH, temperature, light, and oxygen, which can lead to degradation
antioxidants. Even though there are not many studies in the specialist and loss of color and bioactivity (Tarone et al., 2020).
literature, the existing ones show that food products may be functionally Anthocyanin-derived pigments are prone to fading or color changes
enhanced by encapsulated anthocyanin extracts from plants such as during food processing and storage. Preventing color loss and ensuring
eggplant peels. However, more ongoing research is necessary in terms of consistent color in food products is a challenge, particularly in products
purity analysis, safety evaluation, functionality, and sensory proprieties. with extended shelf lives. Developing effective stabilization techniques
Furthermore, regulatory considerations must be considered, as well as to maintain anthocyanin stability in food products is crucial. In addition,
the possible challenges that must be faced. determining the optimal dosage of eggplant peel anthocyanins in food
products to achieve desired health benefits without negatively impact­
7. Regulatory considerations ing taste, texture, or appearance requires careful formulation and testing
(Alongi & Anese, 2021; Roberfroid, 2000).
The use of anthocyanins in the food industry is subject to regulatory Sourcing a consistent supply of high-quality eggplants with desirable
considerations and standards that ensure the safety and quality of food anthocyanin profiles can be challenging, especially for large-scale pro­
products. Regulatory requirements may vary by country or region, so duction. While using eggplant peels can reduce food waste, ensuring
food manufacturers and producers need to comply with local regula­ sustainable sourcing and processing practices to minimize environ­
tions. In general, before introducing to the market food products mental impact is an ongoing challenge. Processing eggplant peels and
enriched with anthocyanins, a thorough safety assessment is typically extracting anthocyanins can be cost-intensive, particularly when opti­
required. This assessment includes toxicological studies for instance to mizing extraction methods and implementing encapsulation techniques.
evaluate the safety of the anthocyanin extracts (EFSA, 2013). Balancing the costs with the potential benefits is a consideration for
In 1982, the Joint FAO/WHO Expert Committee on Food Additives product developers.
(JECFA) recognized anthocyanins as natural food colorants. Later, in Addressing these challenges requires interdisciplinary collaboration
2006, they approved an acceptable daily intake of 2.5 mg/kg/day for among food scientists, agricultural experts, biochemists, and regulatory
anthocyanins from grape skin (Food Safety and Quality: Detail, 2006). authorities. Overcoming these obstacles can unlock the full potential of
Afterward, EFSA granted permission for the incorporation of anthocy­ eggplant peel anthocyanins, making them a valuable resource for
anins under the E163 classification as additives in the EU, aimed at enhancing the nutritional, sensory, and functional attributes of food
enhancing the aesthetic qualities of food and beverages or addressing products while promoting sustainability and health.
color deterioration resulting from processing (EFSA, 2013). However, Additionally, within the context of waste management, it’s essential
the EU regulations do not specify the particular fruits or vegetables that to address the generation of residual biomass during the extraction of
can be employed to derive anthocyanins as a food additive. Further­ bioactive compounds from food waste, as this is a prevalent concern.
more, the composition and specific identity of the anthocyanins that Implementing integrated biorefinery processes offers a sustainable
may be present in the food additive E163 remain undefined (EFSA, approach to utilize this residual biomass by converting it into other
2013). value-added products like biofuels, biochar, and biogas. Alternatively,
In the United States, substances that are Generally Recognized as another method for managing residual biomass is composting, where it
Safe (GRAS) are exempt from the need for pre-market approval as food can be converted into organic fertilizer to enhance soil quality or utilized
additives. Beetroot, carrots, black/purple carrot, chokeberry, Aronia, as animal feed. The adoption of continuous extraction systems, which
elderberry, grape skin, purple sweet potato, red cabbage, red radish, and tend to be more efficient compared to batch processes, or integrating the
saffron extracts were already included in The US National List of extraction process into a broader biorefinery framework can lead to
Allowed and Prohibited Substances as nonorganically produced agri­ reduced waste generation and improved resource utilization. This
cultural products allowed as ingredients in or on processed products approach presents a viable solution worth exploring further (Casau
labeled as “organic” (eCFR). Thus, for the US, it is necessary to et al., 2022; Gupta et al., 2022; Treichel et al., 2020).
demonstrate that eggplant peel anthocyanins fall under GRAS status by Certainly, here are some areas for future research regarding eggplant
ensuring they meet the quality standards for purity, composition, and peel anthocyanins, with a focus on optimization of encapsulation tech­
identity. Analytical methods may be required to verify the content and niques and exploring novel food applications: develop and optimize
authenticity of the anthocyanins. encapsulation methods for eggplant peel anthocyanins to enhance sta­
Food products containing eggplant peel anthocyanins must comply bility, bioavailability, and controlled release;
with labeling regulations. This includes accurate ingredient listing,
declaration of the color additive’s presence, and adherence to any la­ • investigate the synergistic effects of eggplant peel anthocyanins
beling guidelines specific to natural colorants (Regulation (EU) No when encapsulated with other bioactive compounds (e.g., probiotics,
1169/2011). Since eggplants belong to the Solanaceae family, allergen prebiotics, vitamins) in food products for enhanced health benefits;
labeling requirements also must be followed to inform consumers about • assess the impact of encapsulated eggplant peel anthocyanins on the
potential allergenicity. taste, aroma, and texture of food products, aiming to ensure con­
Thus, eggplant peel anthocyanins may be used as natural colorants sumer acceptance;
but following the guidelines of the regulatory authorities on their usage, • assess the shelf-life of food products containing encapsulated an­
including maximum allowable concentrations and permitted color thocyanins, considering variations in temperature, light exposure,
ranges and labeling. and packaging;

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Declaration of competing interest 10.1016/j.tifs.2021.01.003
Condurache, N. N., Aprodu, I., Crăciunescu, O., Tatia, R., Horincar, G., Barbu, V.,
Enachi, E., Râpeanu, G., Bahrim, G. E., Oancea, A., & Stănciuc, N. (2019). Probing
The authors declare that they have no known competing financial the functionality of bioactives from eggplant peel extracts through extraction and
interests or personal relationships that could have appeared to influence microencapsulation in different polymers and whey protein hydrolysates. Food and
the work reported in this paper. Bioprocess Technology, 12(8), 1316–1329. https://doi.org/10.1007/s11947-019-
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