THIRD YEAR MBBS

PATHOLOGY OSPE
BY SYED USAMA QUTBI
MBBS BATCH 25 KMDC
PATTERN OF PRACTICAL VIVA
• COMPONENTS
• CLINICAL
• HISTOPATHOLOGY
• MICRO
• DISTRIBUTION
• GRAND VIVA
• PRACTICAL
• OSPE
• OSPE
• TRANSPARANCIES 10 (4 HISTO, 4 MICRO, 2 CLINICAL)= 1MIN EACH
• SPOTS 3 ( 1 HISTO, 1 MICRO, 1 CLINICAL ) =2-3 MIN EACH
• WRITING SKILLS 2 ( SHORT NOTES ETC)= 2-3 MIN EACH
CLINICAL
PARASITOLOGY
CYST AND TROPHOZOITS OF GIARDIA

Intestine protozoa
Cyst infective stage
Trophozoits in stool
CYST AND TROPHOZOITS OF E. HISTOLYTICA

Intestin protozoa
Cyst infective stage
Trophozoits in stool
AMOEBIC LIVER ABCESS
EGGS OF ENTEROBIUS VERMICULARIS

Intestinal nematode
Eggs infective stage
Scotch tape technique
pinworm
FERTLIZED AND UNFERTILIZED EGGS OF
ASCARIS

Egg infective
stage and in stool
ADULT WORM ASCARIS

Intesti
nematode
EGGS OF ANCYCLOSTOMA
Hook worm
Larva infective stage
Eggs in stool

Segmented lobes in
eggs
EGGS OF TRICHURIS

Whip worm
Infect stage is eggs
Eggs in stool

Bilateral mucoid
plug in eggs
ADULT WORM TRICHURIS

Whip worm
EGGS OF SCHISTOSTOMA MANSONI

Lateral spine
Intermed host, snail
Eggs in stool
EGGS OF SCHISTOSTOMA HAEMATOBIUM

Small terminal spin

IH: Snail
Eggs in stool
EGGS OF TAENIA

Eggs in food n water contim

human faeces. Or larva in
meat is Infect stage
Human stage; adult worm

3 hooklet pairs in egg

GRAVID PROGLOTIDS OF TAENIA SAGINATA

Beef tapeworm
EGGS OF TAENIA SOLIUM

Pork tapeworm
ADULT WORM TAENIA SOLIUM
EGGS OF HYMENOLEPIS NANA

Eggs in faeces
Insects ingest eggs, human
ingest arthropods, infection

4 pair of hooklets in egg

EGGS OF DIPHYLOBOTHRIUM LATUM

Fish
tapeworm
Cause vit B12
deficiency

Eggs have lid

at one end
ADULT WORM ECHINOCOCCUS

Adult tapeworm found in dogs produce eggs, eggs in

dog faeces
Infect stage; eggs in food
Eggs forms hydatid cyst in liver n lung of human

Dwarf tape worm , 3 segments

HYDATID CYST OF ECHINOCOCCUS

Found in liver n lung of

human
GAMETOCYTE STAGE OF P. FALCIPARUM

Banana shape/ crescent shape

Cause tertian malaria
N black water fever
This is giemsa stain
TROPHOZOIT STAGE OF P. VIVAX

Ring shape tropho

FILIRIASIS BY WUCHERIA (ELEPHANTIASIS)

Tissue nematode
By mosquito bite
Infective stage; larva
Adult worm in lymphatics
MATCH STICK TEST FOR DRANCULUS

Organism larva in copepods ingested

by humans
Female worm cause skin blisters
KISSING BUG (REDUVIDD)

Transmit trypanosome cruzi causes Chagas disease

Tryptomastigotes in bug faeces infect human
Amastigotes in heart and neurons
HISTOPATHOLOGY
ACUTE APPENDICITIS
CHRONIC CHOLECYSTITIS
CHOLESTEROLOSIS
XANTHELESMA
GRANULATION TISSUE
KELOID
RENAL INFARCT
CASEOUS NECROSIS
LIQUEFACTIVE NECROSIS
SUPPERATIVE INFLAMMATION
CHRONIC PEPTIC ULCER
FIBRINOUS PERICARDITIS
SEROUS INFLAMMATION
MELANOCYTIC NEVUS
FATTY LIVER
HEPATIC HEMOSIDEROSIS
ACUTE PANCREATIC FAT NECROSIS
HYDROPIC CHANGE (OSMOTIC NEPHROSIS)
GANGRENOUS INFLAMMATION
DEEP VENOUS THROMBOSIS
PULMONARY EMBOLISM
PULMONARY INFARCT
BARETT S ESOPHAGUS
WOUND HEALING
BRAIN ATROPHY
HYPERPLASIA PROSTATE
EDEMA
FOLLICULITIS
DYSTROPHIC CALCIFICATION
ATHEROSCLEROSIS
PETECHIAL HEMORRHAGES
INTRACEREBRAL HEMORRHAGE
SQUAMOUS CELL CARCINOM,A
NUTMEG LIVER
BENIGN CYSTIC TERATOMA
LIPOMA
LEIOMYOMA OF UTERUS
JUVENILE RECTAL POLYP
ADENOCARCINOMA
CAPPILARY HEMANGIOMA
MICROBIOLOGY
GRAM STAIN
GRAM POSITIVE COCCI CLUSTERS

Staphylococcus aureus,
s. epidermitis
GRAM POSITIVE COCCI CHAINS

Strep. pyogenes
GRAM POSITIVE COCCI DIPLOCOCCI

Strep, pneumonia
GRAM POSITIVE RODS CHAINS

B. anthracis
GRAM POSITIVE RODS CLUB SHAPED

Corynebacterium
GRAM POSITIVE RODS BRANCHING RODS

Actinomycetes and nocardia

GRAM NEGATIVE COCCI DIPLOCOCCI

Neisseria
GRAM NEGATIVE RODS STRAIGHT

H influenza
E coli
Salmonella
Shigella
GRAM NEGATIVE RODS CURVED

Compylobacter
H pylori
vinrio
MONOTRICHOUS BACTERIUM
ACID FAST BACILLI ZN STAIN
BLOOD AGAR CHOCOLATE AGAR
no inoculation
BLOOD AGAR alpha HEMOLYSIS
BLOOD AGAR BETA HEMOLYSIS
CHOCOLATE AGAR (inoculated)
MACCONKEYS AGAR lactose fermenter
MACCONKEYS AGAR NON LACTOSE
FERMENTERS
CLED AGAR
LACTOSE FERMENTERS NON LACTOSE FERM
LOWENSTEIN JENSEN MEDIA
NUTRINT AGAR
ROBERTSON COOKED MEAT MEDIUM
TRIPPLE SUGAR IRON MEDIUM TSI
CATALSE TEST

+Ve: Staphylococcus
-Ve: Streptococcus
COAGULASE TEST ( SLIDE / TUBE)
+Ve: Staph Aureus
-Ve: Other Staph
 +Ve: Proteus, H.Pylori
UREASE TEST -Ve: E.coli
OXIDASE TEST

+Ve: Pseudomonas

-Ve: E.coli
INDOLE TEST +Ve: E.coli
-Ve: Enterobacter
 +Ve: Klebsella
CITRATE TEST -Ve: E.coli
CAMP TEST ( Christie atkins munch Peterson
test)
Blood culture bottles
SIGN OF GROWTH
AEROBIC AND ANAEROBIC BLOOD CULTURE
BOTTLES
ANTIBIOTIC SENSITIVITY TEST (KIRBEY BAUER)
ANAEROBIC JAR CANDLE JAR
INCUBATOR
AUTOCLAVE
RED HEAT FLAMING
HOT AIR OVEN
CANDIDA ALBICANS
GERM TUBE TEST CANDIDA
DIRECT MICROSCOPY
DICHOTOMOUS BRANCHING
WIDE / NON SEPTATE RIBBON LIKE
PITYRIASIS VERSICOLOR SHOWING
HYPOPIGMENTED LESIONS BY TENIA VERSICOLOR
DERMATOPHYTOSIS, RING WORM OR TINEA
TINEA UNGUIUM
TENIA PEDIS (AKA ATHLETS FOOT)
TENIA CORPORIS
NAPPY RASH’ spread in infants to the mouth
by candida
Joining chain of IgA
IGM
POSITIVE TUBERCULIN TEST
CELL MEDIATED TYPE IV HYPERSENSITIVITY
LAB DIAGNOSIS OF MALARIA P FALCIPARUM
PERITRICHOUS BACTERIA
PRACTICAL
• IT COMPRISES OF 4 SLIDES
• 2 slides for histopath; one tumor and one non tumorous (identified)
• For each slide
• Write 2 gross feature points , 2 microscopic features points , and draw
diagram,
• 2 slides for micro (non identified)
• One for gram stain and one for ZN stain
• Stool DR (parasite images of cyst or eggs will be given)
FOR GRAM STAIN SLIDE
• Identify the slide and write the following
1. Four to five points of gram stain procedure
2. Identify; gram +ve / -ve
3. Shape: cocci/ bacilli/ curved
4. Arrangement; groups/ chains/ pairs/ or mixed
5. Example; staphylococcus,/ streptococcus/ diplococci/ etc

FOR PARASITO STOOL DR

Images will be given, identify it, draw it, and label it
FOR ZN STAIN SLIDE
• Diagnose it
• Acid fast present or not
• Draw diagram
• Observe puss cells
Gram positive cocci mixed (staph, strep, diplo)
ZN STAIN
GRAM NEGATIVE
THANK YOU