BA-A-S120Mini Software Operating Instructions

BA-A-S120Mini
FULLY AUTO
BIOCHEMISTRY ANALYZER
Version 2023.07.01
User Manual
BA-A-S120Mini Automatic Chemistry Analyzer
Notes
1. Please read through this operating instruction and
keep it properly;
2. Please keep this operating instruction in accessible
place for reading;
3. Please operate this instrument according to operating
manual, otherwise, it may cause severe consequences.
User Manual of BA-A-S120Mini Analyzer
Catalogue
Chapter 1 Introduction..... ............................................................................................................4
1.1 Product overview ............................................................................................................................................ 4
1.1.1 Product introduction...................................................................................................................4
1.1.2 Basic performance.......................................................................................................................5
1.1.3 Main structure..............................................................................................................................5
1.1.4 Scope of application.....................................................................................................................5
1.2 Symbol, chart displays, label and picture .................................................................................................... 6
1.2.1 Symbol...........................................................................................................................................6
1.2.2 Chart display and label......... .....................................................................................................7
1.2.3 Picture...........................................................................................................................................7
1.3 Warranty and service ..................................................................................................................................... 7
1.4 Training ........................................................................................................................................................... 8
1.5 Explanation of terms .................................................................................................................... 9
1.6 Explanation of terms .................................................................................................................... 9
Chapter 2 Basic operation Procedure ..................................................................................................... 15

2.1 Adding Item Parameters .................................................................................................................................................... 15
2.2 Modifying Item Parameters ............................................................................................................................................... 15
2.3 Deleting Items ....................................................................................................................................................................15
2.4 Adding QC solutions ..........................................................................................................................................................16
2.5 Modifying QC solutions .................................................................................................................................................... 16
2.6 Deleting QC solutions ........................................................................................................................................................16
2.7 Adding CAL solutions ....................................................................................................................................................... 16
2.8 Modifying CAL solutions ..................................................................................................................................................16
2.9 Deleting CAL solution .......................................................................................................................................................16
2.10 Setting Reagent Positions ................................................................................................................................................ 17
2.11 Changing Reagent Positions ............................................................................................................................................ 17
2.12 Clearing Reagent Positions ..............................................................................................................................................17
Chapter 3 Basic Test Procedure ................................................................................................ 18
3.1 Reagent Black Test Procedure .................................................................................................................... 18
3.2 CAL Test Procedure .....................................................................................................................................18
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3.3 General Test Procedure ............................................................................................................................... 19
3.4 QC Test Procedure ....................................................................................................................................... 20
Chapter 4 Operational procedure for new items ..................................................................... 23
Chapter 5 Routine operational procedure ................................................................................24
5.1 Check before Power-on ................................................................................................................................ 24
5.2 Power-on ........................................................................................................................................................ 24
5.3 Run the Program .......................................................................................................................................... 24
5.4 Test request ................................................................................................................................................... 24
5.5 Test preparation ............................................................................................................................................24
5.6 Start the test .................................................................................................................................................. 25
5.7 Test results confirmation ............................................................................................................................. 25
5.8 Exit the program ...........................................................................................................................................25
5.9 Power-off ........................................................................................................................................................26
5.10 Check after power-off ................................................................................................................................ 26
Chapter 6 Interface operation ....................................................................................................27
6.1 Login interface .............................................................................................................................................. 27
6.2 General setting .............................................................................................................................................. 33
6.3 Test setting-up ...............................................................................................................................................47
6.4 Statistics/Query ............................................................................................................................................. 59
6.5 Maintenance .................................................................................................................................................. 65
6.6 Shortcut buttons ............................................................................................................................................76
6.7 Instrument Running Status Diagram ......................................................................................................... 91
Chapter 7 Computing method ................................................................................................... 92
7.1 Test process ................................................................................................................................................... 92
7.2 Metering point ...............................................................................................................................................92
7.3 Analytical method .........................................................................................................................................92
7.4 Absorbance and reaction amplitude ...........................................................................................................94
7.5 Calibration .....................................................................................................................................................95
7.6 Concentration calculation ............................................................................................................................96
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7.7 QC ...................................................................................................................................................................96
7.8 Other Relevant Computation ...................................................................................................................... 98
Chapter 8 Abnormal Model and Cause Analysis of Common Results ................................ 103
8.1 Reactive Curve Fluctuates and Beats ....................................................................................................... 103
8.2 Abnormal Reaction Curve .........................................................................................................................103
8.3 Normal Reaction Curve, Poor Repeatability of the Results ...................................................................104
8.4 Normal Reaction Curve, Results Is Zero or Even Lower ...................................................................... 104
8.5 Normal Reaction Curve, Results Falls Outside QC ................................................................................104
8.6 Normal Reaction Curve, Results are Higher Or Lower Generally .......................................................105
Chapter 9 Common Operation and Regular Maintenance ...................................................106
9.1 Preparation of tools .................................................................................................................................... 106
9.2 Common Operations .................................................................................................................................. 106
9.3 Daily Maintenance ...................................................................................................................................... 106
9.4 Weekly Maintenance .................................................................................................................................. 108
9.5 Monthly Maintenance ................................................................................................................................ 109
9.6 Unscheduled Maintenance ......................................................................................................................... 110
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Chapter 1 Introduction
1.1Product overview
1.1.1Product introduction
Product name: Automatic chemistry analyzer;
Model: BA-A-S120Mini;
Safety classification: Electric shock resistance grade I, overvoltage grade II, pollution grade 2;
Management classification: It belongs to biochemical analysis system in clinical examination analytical

instrument (6840), management grade II.
1.1.2Basic performance
Central wavelength
No more than ±2nm
deviation
Semi-wave width No more than 12nm
Sdisk light Absorbance no less than 2.5
Error no bigger than ±0.020 when absorbance is 0.5, Error no bigger than
Absorbance accuracy
±0.04 when absorbance is 1.0
Absorbance
Variable coefficient no bigger than 1.5%
repeatability
Temperature accuracy Temperature accuracy should be ±0.2 ℃ , temperature fluctuation should be

and fluctuation ±0.1℃
Adding sample error ≤±4%, variable coefficient ≤2% when sample is 2

microliter; Adding sample error ≤±4%, variable coefficient ≤2% when sample
is 5 microliter; Adding sample error ≤±4%, variable coefficient ≤2% when
Adding sample error
and variable coefficient sample is 30 microliter; Adding sample error ≤±3%, variable coefficient ≤2%
when reagent is 20 microliter; Adding sample error ≤±3%, variable coefficient
≤2% when reagent is 300 microliter;
Input power 150W
Dimension L×W×H：350×605×330mm
Net weight 22 Kg
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1.1.3Main structure
BA-A-S120Mini automatic chemistry analyzer consists of analysis unit and operation unit. The analysis unit
includes sample disk, sample absorbing mechanism, reagent disk, reagent absorbing mechanism, reaction disk,
reaction tank, mixing mechanism, cuvette cleaning mechanism, and photometer; operation software is installed
in computer or touch screen, it drives and controls analysis unit to perform all analysis actions.
1.1.4Scope of application
It is applicable to biochemical detection on human body serum, plasma and urine specimen performed by
medical institution.
Note:
This operation manual may be modified without prior notice.
1.2Symbol, chart displays, label and picture
1.2.1Symbol
Symbols listed below are applicable to this operation manual.
Symbol Meaning
Attention! Risk of damaging analyzer or affecting test result.
Risk of biological pollution.
Risk of electric shock.
Risk of corrosion injury.
Hot! Risk of injury to human body.
Strong light! It may hurt eyes.
Risk of injury to human body.
Flammable! Risk of fire.
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1.2.2Chart displays and label

Chart displays and label are listed below and applicable to this operation manual.
Label Meaning
Product serial No.
Equal potential terminal
Product manufacturing date
In vitro diagnosis
～ Alternating current
Power on
Power off
CHEMISTRY ANALYZER Chemistry analyzer

MAIN POWER Main power
ANALYZING UNIT POWER Analyzing unit power
ON Power on
OFF Power off
COM R232
Communication port
Serial port
WASTE
Waste outlet
Waste outlet
PURIFIED WATER
Purified water inlet
Purified water inlet
1.2.3Picture
All pictures in this operating manual are for instruction or example only but not for other purposes.
1.3Warranty and service

BA-A-S120Mini automatic chemistry analyzer will have one year warranty for free since the date of
installation. But damages caused by the following circumstances will not be included in free maintenance:
(1) Operating environment does not conform to operation manual;
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(2) Use of illegal power or power failure;
(3) Artificial damage;
(4) Maintenance performed by personnel without our company’s authorization.
(5) Force majeure, such as earthquake, fire, or war;
1.4Training
For proper use of BA-A-S120Mini automatic chemistry analyzer and give full play to its performance,
our company holds free user training regularly.
For training schedule, please visit our company website or contact local office.
Note: this operation manual may be revised without prior notice which may cause slight difference from the
real instrument. Specific parameters and pictures should subject to real object and operating software.
1.5 Explanation of terms

1.5.1 AD value
The light current generated after the light reaches the detector will flow through a fixed resistance, and convert into a
light voltage (analog signal) after amplification. This voltage will convert into a numerical signal with the
corresponding magnitude (the magnitude is related to the selected number of bits of AD) after AD conversion
(analog-digital conversion), and this value is the AD value.
1.5.2 Dark current

The numerical signal which is output by the photoelectric detector when the light source is not open (i.e., when
there is no signal light) can be expressed as AD value. The dark current is equivalent to the circuit background,
and it must be deducted when calculating the absorbance.
1.5.3 Water blank

It refers to the absorbance value obtained when the reaction cup is filled with distilled water. Since the
absorbance value is relative, that is, based on a certain absorbance value, in the SL 120, the absorbance of
water blank is defined as 0, which means any other absorbance must subtract the absorbance value obtained
when the reaction cup is filled with distilled water.
1.5.4 Metering point

The specific time for conducting the photoelectric colorimetry are generally expressed as the specific
numerical values, and there is a strict but fixed time relationship between various metering points. In the SL
120, there is a total of “n+m” metering points for each reaction (“n” is the endpoint number set in “Item
Parameter”, “m” is configurable number of points collected continuously after the endpoint).
1.5.5 Absorbance
It refers to the common logarithm obtained from taking the negative number after the transmitted light
intensity divides the incident light intensity. In the SL 120, the incident light intensity is the AD value obtained
when the reaction cup is filled with distilled water, and the displayed absorbance value is the calculated
absorbance value ×104.
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1.5.6 Reaction curve

It refers to a series of points composed by taking the metering point as the horizontal coordinate and taking the
absorbance as the vertical coordinate. The typical reaction curve of SL 120 is shown as follows:
1.5.7 Reaction amplitude

It refers to the change in absorbance before and after the reaction or the change rate of absorbance during the
reaction.
1.5.8 Calibration
Also refers to calibration. For the sample (also known as “calibrator”) with one or more known concentration
(or activity), measure its reaction magnitude; by depending on the selected calibration mode (linear or
nonlinear), carry out fitting by using an optimal curve-paired data set (concentration, reaction magnitude), and
calculate the mathematical expression of this curve. By using this curve, measure the reaction amplitude of the
sample with an unknown concentration (or activity), which can calculate the concentration (or activity) of this
sample.
1.5.9 Calibration curve

It refers to a series of points composed by taking the concentration (or activity) as the horizontal coordinate
and taking the reaction magnitude as the vertical coordinate, and a curve fitted by using an optimal
mathematical equation.
1.5.10 Calibration parameter

It refers to other items except concentration and reaction amplitude in the expression of calibration curve.
The following are the warning signs of BA-A-S120Mini automatic chemistry

analyzer. Ignoring such signs may cause sever consequences or injury to the users.
The following signs are not in order of importance, all signs are equally important.
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1.6 Safety
Electric shock
(1). When power on, unauthorized maintenance personnel are not allowed to open
instrument panel, side cover or rear cover;
(2). Do not spill liquid on the table. If liquid flows inside the instrument, please power off
immediately
(3). High voltage maintained inside computer and printer. Do not touch the inside of
computer or printer.
Injury
(1). Please pay attention to sharp parts of the analyzer, such as reagent tip, sample tip,
mixing rod, or steel tube of automatic cleaning mechanism, otherwise it may cause
injury;
(2). When analyzer is in movement, do not touch its moving parts, such as sample tip,
reagent tip, mixing rod, automatic cleaning mechanism, fan etc.
Hot
(1). Before replacing bulb, please turn off power switch and wait for at least 30 minutes
until bulb cools down totally;
(2). Touching the printer head or metal around the printer head may cause burn injury.
Strong light
(1). Do not look at light beam of bar code scanner directly. These light beams will cause
injury to the eyes;
(2). Do not look at the bulb light directly. These light beams will cause injury to your
eyes.
Biological contamination
(1). All test samples, calibrated products and quality control products are considered
infectious. Wear gloves when you are handling it;
(2). All waste liquid, reagent bottles, overdue reagent and disposable test tubes will be
considered infectious. Discharge or disposal of such object should follow local
sanitation and environmental protection and administration rules, laws and
regulations.
(3). All objects exposed to test samples such as reagent tip, sample tip, mixing rod,
cleaning mechanism, waste liquid tube and bucket will be considered infectious.
Wear gloves while handling them.
Corrosion
(1). Cleaning agent and some reagents have strong acidity or alkaline. Do not touch
with hands or clothes. Once exposed to such material, clean using water and soap;
(2). If it gets into eyes, rinse thoroughly with clean water and consult
ophthalmologist.
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Burn
(1). Do not use flammable hazardous article around analyzer, such as ethyl alcohol
and diethyl ether.
Explosion
(1). Do not use explosive articles around analyzer.
Precautions
Scope of application
(1). BA-A-S120Mini automatic chemistry analyzer is applicable to chemistry
detection on human body serum, plasma and urine specimen performed by
medical institution. When making clinical decisions based on test result, please
correlate with clinical conditions or other test results.
Operator
(1). BA-A-S120Mini automatic chemistry analyzer should only be operated by
personnel trained
Application environment
(1). Please install in an environment designated by the operating instruction.
Installation or use of the instrument in other conditions may produce unreliable
results and cause damage to the analyzer;
(2). If you need to change operating environment of the analyzer,
please contact local distributor.
Electromagnetic interference
（1） Analyzer will receive electromagnetic interference during operation, which may
affect measurements and cause malfunction. Do not use devices which may
produce electromagnetic wave during use, such as electric drill, mobile phone and
walkie-talkie near the analyzer.
（2） During operation, analyzer will send electromagnetic wave externally. Do not
install or use electromagnetic sensitive device around the analyzer.
Imperfect earth
(1). Power supply must be earthed correctly, otherwise it may cause electric shock;
(2). Ground impedance must be smaller than 10Ω. Imperfect earthing will cause
inaccurate measurements or leakage and result in electric shock.
Liquid leakage
(1). Before testing, please check each pipe joint carefully for any leakage. Liquid
leakage will cause inaccurate absorption or discharge amount;
(2). Do not place reagents or samples on analyzer table to prevent liquid spill or
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leakage.
Blockage
(1). Check reagents and samples carefully. There should be no insoluble floats, such
as cellulose and fibrous protein, otherwise it may cause reagent/sample blockage.
Water quality
(1). Water quality should meet the requirements below, otherwise it may cause
damage to valve or pump, or unable to clean.
 Particle diameter smaller than 200 micrometer;
 Electrical resistivity higher than 0.5MΩcm;
 Clump count fewer than 10cfu/ml;
 Dissolved silicate fewer than 0.1mg/L.
Analysis parameter
(1). Incorrect analysis parameter will cause wrong measurements.
Please consult Bioevopek Ltd.
1.6.1 Transportation and storage condition

Transport and store under low temperature: 0℃
Transport and store under high temperature: 55℃
1.6.2 Electromagnetic compatibility
Attention:
 BA-A-S120Mini automatic chemistry analyzer meets with requirement on emission and noise immunity
in GB/T 18268.26, see table below.
 User is responsible to make sure electromagnetic compatibility environment of the device so that device
functions well.
 We suggest assess electromagnetic environment of the device before use.
Warning:
 BA-A-S120Mini automatic chemistry analyzer is designed and detected according to category A device in
GB 4824. In domestic environment, this device may cause radio interference. Protective measures should
be taken.
 Do not use this device nearby strong radiation source (e.g., unshielded RF source), otherwise it may
disturb device function.
Table 1:
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Electromagnetic emission
Emission test Conformity
GB 4824 RF emission Group 1
GB 4824 RF emission Category A
GB 17625.1 Harmonic wave emission Not Applicable
GB 17625.2 Voltage fluctuation/flicker emission Not Applicable
Table 2:
Electromagnetism noise immunity
Immunity test item Primary Test value Performance

standard criterion
Electrostatic discharge Contact discharge:±2kV, ±4kV

GB/T 17626.2 B
(ESD) Air discharge:±2kV, ±4kV,±8kV
Radio frequency
GB/T 17626.3 3V/m, 80MHz～2.0GHz, 80%AM A
electromagnetic field
Power line:±1kV(5/50ns,5kHz)
Pulse group GB/T 17626.4 B
I/0 signal line: ±0.5kV(5/50ns,5kHz)
Line-to-earth: ±2kV
Surge GB/T 17626.5 B
Line-to-line: ±1kV
Power line:3V/m, 150kHz～80MHz, 80%AM

Radio frequency
GB/T 17626.6 I/0 signal line: 3V/m, 150kHz ～ 80MHz, A
transmission
80%AM
Power frequency
GB/T 17626.8 3A/m, 50/60Hz A
magnetic field
1 period 0%; B
Voltage sag, 5/6 period 40%; C

GB/T 17626.11
interruption 25/30 period 70%; C
250/180 period 5% C
Performance determination:
A. During test, device functions well within standard limit.
B. During test, function or performance compromises or loses but it recovers automatically.
C. During test, function or performance compromises or loses. It requires operator to intervene or reset
system.
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Chapter 2 Basic Operation Procedure

2.1 Adding Item Parameters
 For the reagent open system:
1. Click the shortcut “Open menu” in the main interface to display the main menu;
2. Click “Test settings” in the main menu, and select “Item parameters” from the drop-down menu
to display the item parameter interface;
3. Click “New”, enter measured parameters according to the meanings of parameter items and the
reagent instructions;
4. Click “Save”.
 For the reagent closed system:
3. Click “Read card”, and the system prompts the user to put an IC card in a specified position to
read measured parameters to the system from the IC card for test use.
2.2 Modifying Item Parameters

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3. Select items to be modified. Click “Modify” to modify measured parameters according to the
meanings of parameter items and the reagent instructions;
Item parameters can only be sourced from IC cards provided by the reagent manufacturer. The user
cannot modify item parameters.
2.3 Deleting Items

3. Select items to be deleted. Click “Delete” to display a new prompt interface;
4. Click “Yes”.
The user cannot delete item parameters.
2.4 Adding Quality Controlled Solutions

2. Click “Test settings” in the main menu, and select “Quality control settings” from the drop-down menu
to display the quality control setting interface;
3. Click “New”, enter the following contents according to the meanings of parameter items: name, the
target value and standard deviation of items, and cumulative sum control rules;
2.5 Modifying Quality Controlled Solutions

3. Select quality control solutions to be modified. Click “Modify” to modify the following contents
according to the meanings of parameter items: name, the target value and standard deviation of items,
and cumulative sum control rules;
2.6 Deleting Quality Controlled Solutions

3. Select quality control solutions to be deleted. Click “Delete” to display a new prompt interface;
4. Click “Yes”.
2.7 Adding Calibration Solutions

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2. Click “Test settings” in the main menu, and select “Calibration settings” from the drop-down menu to
display the calibration setting interface;
3. Click “New” to enter the following contents according to the meanings of parameter items: name, the
standard value of items, and position on the calibration tray;
2.8 Modifying Calibration Solutions

3. Select calibration solutions to be modified. Click “Modify” to modify the following contents according
to the meanings of parameter items: name, the standard value of items, and position on the calibration
tray;
2.9 Deleting Calibration Solutions

3. Select calibration solutions to be deleted. Click “Delete” to display a new prompt interface;
4. Click “Yes”.
2.10 Setting Reagent Positions

1. Click the shortcut button “Reagent tray”;
2. Switch the bookmark to a No. of reagent tray required;
3. Select a reagent name whose reagent position will be set. For dual reagent, separately select R1 and R2;
4. Double-click an empty reagent position, and the selected reagent is set to this position.
2.11 Changing Reagent Positions

2. Switch the bookmark to the reagent disk needed;
3. On the reagent tray, click a reagent name whose reagent position will be changed;
4. Double-click an empty reagent position, and the selected reagent is changed to this position from the
original position.
2.12 Clearing Reagent Positions

2. Switch the bookmark to the reagent disk needed;
3. On the reagent tray, click a reagent name to be cleared;
4. Click “Delete occupied cup positions” to display a new prompt interface;
5. Click “Yes”.
6. On the graphical reagent position of the reagent tray, right-click to display a menu. There are two items
in the menu as follows: “Release a single reagent position” and “Release all reagent positions on the
tray”.“Release a single reagent position”: Release the current clicked reagent position; “Release all
reagent positions on the tray”: Release all occupied reagent positions on the current selected reagent
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tray.“Release a single reagent position” can be activated only by right-clicking on an occupied position.
Chapter 3 Basic Test Procedure

3.1 Reagent Black Test Procedure
(1) Check reagent positions, and ensure that all reagent bottle caps are open;
(2) Click the shortcut button “Start” in the main interface. Enter start conditions in the displayed interface;
(3) Select reagent tray numbers (1-5) to be tested;
Note：
If a reagent tray needs to be changed over, it can be performed only after testing on
the current tray is finished.
(4) Click the “Reagent blank” column. Select a reagent to be tested and make a confirmation (Clicking
once indicates selection; clicking once more indicates cancellation). When “Automatically test a
reagent blank” is checked, the reagent blank can be automatically tested only once a day. If reagent
blank test results of this item are not ideal, you can use the button “Reagent blank test>>” to select a
test item that will be used as a reagent blank, and perform reagent blank testing on the selected test
item.
(5) Click the button “OK”.
Note：
If the pail icon at the top left corner of the main interface becomes red and
constantly flashes during testing, add purified water in the purified water pail
immediately, otherwise it will lead to abnormal results.
3.2 Calibration Test Procedure

(1) Click the shortcut button “Calibration application” in the main interface to display the calibration
application interface. Select an item to be calibrated, a calibration solution required for this item, and
calibration rules of this item;
(2) Repeat the previous step until all items to be calibrated are selected;
(3) Click “Requisition”;
(4) Put calibration solutions on the sample tray according to positions applied for;
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Note：
If a micro cup is used, the liquid level of serous must be ensured to be not less than
8mm, otherwise it is liable to liquid level detection errors, affecting calibration
results.
(5) Recheck reagent positions, and ensure that all reagent bottle caps are open;
(6) Click the shortcut button “Start” in the main interface. Enter start conditions in the displayed interface;
(7) Select reagent tray numbers (1-5) to be tested;
Note：
(8) If you want to need to automatically test a reagent blank, tick before “Automatically test a reagent
blank”, otherwise proceed to the next step;
Note：
Automatically performing reagent blank testing may eliminate the effect of reagent
change on results to some extent. This function is equivalent to the blank calibration
of the Hitachi and Olympus machine types.
(9) If you do not select “Automatically test a reagent blank” but want to separately measure a reagent
blank of an individual item, click the “Reagent blank” column. Select a reagent to be tested and make a
confirmation (Clicking once indicates selection; clicking once more indicates cancellation);
Note：
For some unstable reagents such as ALP, AMY, GGT and Cr, it is recommended to
test their reagent blanks once a day.
(10) Click the “Calibration test” column (Clicking once indicates selection; clicking once more indicates
cancellation);
Note：
If the pail icon at the top right corner of the main interface becomes red and
constantly flashes during testing, add purified water in the purified water pail
immediately, otherwise it will lead to abnormal results.
3.3 General Test Procedure

1. Click the shortcut button “Test application” on the left of the main interface;
2. Enter a sample code in the test application interface. Select sample tray numbers (1-4) and sample
positions (1-24). Select items or item combinations (Clicking once indicates selection; clicking twice
indicates cancellation). Click the button “Apply for” below.
Note：
Calculation items are incorporated in item combinations. The system can
automatically calculate corresponding calculation items such as globulin only after
item combinations such as liver function are clicked.
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3. Repeat the previous step until all samples are applied for. After application is finished, return to the
main interface.
4. Click the sample tray. In the displayed new interface, click samples on the sample tray. Carefully check
sample positions as well as items applied for to ensure consistency with the checklist.
Note：
(1) If you want to delete a sample, after clicking this sample in the sample tray
interface, right-click and then click “Delete a single sample”.
(2) If the application for a sample is in error or you want to increase or reduce test
items of a sample, delete this sample first. After returning to the main interface,
click the shortcut button “Test application” or “High-speed test” at the top left
of the main interface (It is necessary to be consistent with the last application
status of the deleted sample). Enter the sample code of the deleted sample in the
test application interface, and just reapply.
5. Place samples on the sample tray according to positions applied for.
Note：
(1) If a blood sampling tube is directly used, check carefully and ensure that serum
has been completely separated and there is no fibrous protein in serum,
otherwise the needle is liable to blockage.
(2) If a micro cup is used, the liquid level of serous must be ensured to be not less
than 8mm, otherwise it is liable to liquid level detection errors, affecting
calibration results.
6. Recheck reagent positions, and ensure that all reagent bottle caps are open;
7. Click the shortcut button “Start” in the main interface. Enter start conditions in the displayed interface;
(1) Select reagent tray numbers (1-5) and sample tray numbers (1-4) to be tested.
Note：
If a reagent or sample tray needs to be changed over, it can be performed only after
testing on the current tray is finished.
(2) If you want to need to automatically test a reagent blank, tick before “Automatically test a reagent
blank”, otherwise proceed to the next step;
Note：
Automatically performing reagent blank testing may eliminate the effect of reagent change
on results to some extent. This function is equivalent to the blank calibration of the
Hitachi and Olympus machine types.
(3) If you do not select “Automatically test a reagent blank” but want to separately measure a reagent
blank of an individual item, click the “Reagent blank” column. Select a reagent to be tested and make a
confirmation (Clicking once indicates selection; clicking once more indicates cancellation);
Note：
For some unstable reagents such as ALP, AMY, GGT and Cr, it is recommended
to test their reagent blanks once a day.
(4) If quality control testing is included in this lot of tests, click the “Quality control” column (Clicking
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once indicates selection; clicking once more indicates cancellation);

(5) Click the “General test” column (Clicking once indicates selection; clicking once more indicates
cancellation). Enter an initial number of a desired general test in the following input box. In case of no
input, the system starts all general tests by default;
Note：
If the pail icon at the top left corner of the main interface becomes red and constantly
flashes during testing, add purified water in the purified water pail immediately, otherwise
it will lead to abnormal results.
3.4 Quality Controlled Test Procedure

1. Click the shortcut button “Test application” at the top left of the main interface;
2. Click the quality control application radio on the left. Select a quality control solution to be applied for.
Select sample tray numbers (1-4) and sample positions (1-24). Select items or item combinations
(Clicking once indicates selection; clicking twice indicates cancellation). Click the button “Apply for”
below.
3. Repeat the previous step until all quality control applications are finished. - After application is finished,
return to the main interface.
4. Click the sample tray. In the displayed new interface, click the quality control test on the sample tray.
Carefully check application items of each quality control solution to ensure that applications are
correct.
Note：
(1) If you want to delete a quality control test, after clicking this quality test on the
sample tray interface, right-click and then click “Delete a single sample”.
(2) If the application for a quality control solution is in error or you want to increase
or reduce test items of a quality control, delete this quality control test first. After
returning to the main interface, click the shortcut button “Test application” or
“High-speed test” at the top left of the main interface (It is necessary to be consistent
with the last application status of the deleted sample). Just reapply in the test
application interfce.
5. Place the quality control solution in the sample disk according to the applied position.
Note：
If a micro cup is used, the liquid level of serous must be ensured to be not less than
8mm, otherwise it is liable to liquid level detection errors, affecting calibration
results.
- 19 -
6. Recheck reagent positions, and ensure that all reagent bottle caps are open;
7. Click the shortcut button “Start” in the main interface. Enter start conditions in the displayed interface;
(1) Select the number (1-5)of reagent disks and the number (1-4)of sample disk that intended to start test;
Note：
(2) If you need the reagent blank can be tested automatically, please tick in the box in front of
“Automatically test reagent blank”, otherwise directly enter into next step.
Note：
Automatically performing reagent blank testing may eliminate the effect of reagent
change on results to some extent. This function is equivalent to the blank calibration of the
Hitachi and Olympus machine types.
(3) If you do not select “Automatically test reagent blank” and want to test the reagent blank of individual
item, click “Reagent Blank” and select the reagent intended to test, then click “OK” (clicking one time
for selected, and one time again for cancel);
Note：
For some unstable reagents such as ALP, AMY, GGT and Cr, it is recommended
to test their reagent blanks once a day.
(4) Click the “Quality control test" column (Clicking once indicates selection; clicking once more indicates
cancellation);
(5) If general testing is included in this lot of tests, click the “General test” column (Clicking once
indicates selection; clicking once more indicates cancellation). Enter an initial number of a desired
general test in the following input box. In case of no input, the system starts all general tests by default;
- 20 -
Note：
f the pail icon at the top right corner of the main interface becomes red and constantly
flashes during testing, add purified water in the purified water pail immediately, otherwise
it will lead to abnormal results.
Chapter 4 Operational procedures for new items

1 Add the measured parameter to the item;
2 Set the reagent position to the item;
3 Add the calibration solution to the item, or add item concentration to the existing calibration solution;
4 Add the quality control(QC) solution to the item, or add the item target value and standard deviation to
the existing quality control;
5 Make a calibration request for the item;
6 Click the shortcut “Start” button at the main interface and click “Calibration Test”, then confirm it;
7 When the calibration is completed successfully, make a QC request and run the test;
8 The general sample test can be carried out when the QC is performed.
- 21 -
Chapter 5 Routine operational procedures

5.1 Checks before Power-on
1. Check that printer paper is sufficiently available. If not, add printer paper;
2. Check that the power source is in good condition and reliably connected;
3. Check that the cables between the printer and computer and between the computer and instruments are
reliably connected;
4. Open the front door of the analyzer to check if there is purified water. Add purified water immediately
if it is insufficient;
5. Empty the waste barrel, and check that the waste liquid can be discharged properly;
6. Check the sampling probe, stirring rod and pipe of the cleaner mechanism. Wipe off any dirt on them
with cotton wool absorbing alcohol absolute;
7. Check the reagent injector and sample injector for air bubbles and leaks;
8. Remove the remaining sample on the sample tray;
9. Take out the reagent tray from the fridge and put it into reagent cabinet. Open the reagent bottle
5.2 Power-on
Turn the power on in the order below:
1. Press the “Power” button on the right-hand side of the analyzer(The power indicator light will be on);
2. Power up the display;
3. Power up the main unit;
4. Power up the printer.
5.3 Run the program

Double click the “Analyzer System” icon on the desktop. Choose the ID and type in the password to start the
analyzer system: all movement parts are reset; the reaction tray is heated up. Preheating varies between 20 and
40 minutes (depending on the ambient temperature);
5.4 Test requests

1. Reagent blank request;
2. Calibration request;
3. QC request;
4. General test request.
- 22 -
5.5 Test preparation

1. Place the reagent properly on the reagent tray according to the reagent position. Add the reagent if the
remaining reagent is insufficient;
2. Place the calibration solution properly on the sample tray according to the calibration solution position;
3. Place the QC solution properly on the sample tray according to the requested position;
4. Place the sample on the sample tray according to the requested position.
5.6 Start the test

1. Click the shortcut “Start” button;
2. Select the test type, including reagent blank test, calibration test, QC test and general test;
3. Input the code range of the sample to be tested;
4. Click “Confirm” button.
5.7 Test results confirmation

1. Reagent blank result confirmation:
a) Click the shortcut “Open menu” in the main interface to display the main menu;
b) Click “Statistic/query” on the main menu. Select “Reagent blank query” in the drop-down list.
Then the reagent blank query interface will jump out;
c) Input the date range and select the item to be queried before clicking the “Query” button in lower
left-hand corner. Then all the reagent blank entries that meet the query conditions will be listed on
the right-hand side of the interface;
d) Select a certain calibration entry, and click the “reaction curve” button on the lower right-hand
corner to display the reaction process graph of the reagent blank. Select the “Set Default” to set
the selected reagent blank result at the current reagent parameter value of the item.
2. Calibration Result Confirmation:
a) Click the shortcut “Open menu” in the main interface to display the main menu;
b) Click “Statistic/query” on the main menu. Select “Calibration result query” in the drop-down list.
Then the calibration query interface will jump out;
c) Input the date range and select the item to be queried before clicking “Query” in lower left-hand
corner. Then all the calibration entries that meet the query conditions will be listed on the
right-hand side of the interface;
d) Select a certain calibration entry, and click “details” on the lower right-hand corner to display the
detailed information of the calibration entry.
3. QC Result Confirmation:
a) Click the shortcut “View Results” button on the main interface;
b) Click the name of the QC solution in the lower left-hand corner of the interface to view all the
requested items of this particular QC solution. Selected a certain completed item, and click the
lower shortcut “reaction curve” to view the reaction curve and data of this item.
4. General test result confirmation:
a) Click the shortcut “View Results” button on the main interface;
b) Click the sample number to be queried on the left-hand side, and all the requested items of the
sample will be listed in the lower part of the interface. Select a certain completed item, and click
the lower shortcut “reaction curve” button to view the reaction curve and data of this item.
c) To input the sample’s patient information, select the sample number and key in the patient
- 23 -
information on the interface. Then click the “save” button in the upper left-hand corner.
d) To redo the test of a item, select the “Re-test” option on the right-hand side of the results bar(click
to select, and click again to de-select). Or you may click “select all” to select all the tested items of
the sample, and then click “Start and Select Re-test”.
5.8 Exit the program

Click the shortcut “Normal Exit” button on the main interface. The exit in the order below normally lasts 10
minutes:
1. Reset all movement parts of the instrument.
2. Exit the program.
Note：
In case of serious errors during the test which requires emergency exit, you may turn
the power off on the left-hand side of the analyzer, and click the shortcut
“Emergency Exit” button on the main interface.
5.9 Power-off
Power off in the order below:
1. The instrument;
2. The printer;
3. The computer;
4. The display.
5.10 Checks after power-off

1. Remove the sample, calibration solution and QC solution from the sample tray;
2. Close the reagent bottle. Remove the reagent tray and put it in the fridge;
3. Wipe off the analyzer table;
4. Check the sampling probe, stirring rod and pipe of the cleaner mechanism. Wipe off any dirt on them
with cotton wool absorbing alcohol absolute;
5. Check the reagent injector and sample injector for air bubbles and leaks.
- 24 -
Chapter 6 Interface operation

6.1 Login interface
It includes login main interface, communication parameter setting, data source setting and data backup setting,
which are described below in detail:
6.1.1 Login main interface
(1) Parameter explanation
Parameters Meanings Operations
Login user name The user name to log in the Select in the drop-down
instrument system list after it.
User password The password to log in the Type in the password in

instrument system the square frame after it.
(2) Operational Order
Log in the operation system of the instrument
1 Select the correct data source;
2 Select the user name;
3 Input the password corresponding to the user name;
- 25 -
3 To log in, click “Confirm”. Or else, click “Cancel”.
6.1.2 Communication parameters setting

Click “Communication Setting” on the “Login Main Interface”, then the following interface contents will
show up.
Serial port selection The serial port number of the PC Select in the drop-down
connected to the instrument list after it. Select the
serial port number as it
is indicated on the
computer
Start Position The start bit length of Select in the drop-down

communication data in the frame list after it. 1-digit start
format bit is used in this
system.
Stop Position The stop bit length of Select in the drop-down

communication data in the frame list after it.1-digit start
system.
Data bit The data bit length of Select in the drop-down

communication data in the frame list after it.8-digit start
system.
Parity check bit The data check bit of Select in the drop-down
communication data in the frame list after it.”No check” is
format used in this system.
- 26 -
Baud rate The speed at which the Select in the drop-down

communication data are list after it. “19200” is
transmitted used in this system。
Length of sending Length of the Send Buffer Zone Type in the square frame
area (number of bytes) during data after it. The default
transmissions value is “512”
Length of receiving Length of the Receive Buffer Type in the square frame
area Zone (number of bytes) during after it. The default
data transmissions value is “4096”
Communication Length of the command queue Type in the square frame

thread instruction in the communication thread after it. The default
queue length value is “5000”
(2) Basic processing sequence
Communication Parameters Adjustment
1 Adjust some of the above parameter items according to the actual parameters;
2 Click the “Default value” button to change all parameters to the default
values, and click the “Confirm” button to complete the changes to
parameters. Click the “Cancel” button to keep the parameters unchanged.
- 27 -
6.1.4 Setting of parameters of automatic backup data

Click “Display Backup” on the “Login Main Interface”, then the following 2 interface contents will show up.
As only the administrator has the authority to change the parameters of backup data, the administrator identity
validation is required before any changes can be made. There are two kinds of backup, automatic and manual.
- 28 -
Automatic backup file The backup will take place when Designate the path for
path: the operational software is run automatic backup by
for the first time each day. means of selecting in the
Therefore, the path for “drive” and “directory”
automatic backup needs to be lists.
designated, and is preferably
located on other than system
drive. As in the case of system
failure, which requires
re-installation of the system, the
backup data are lost and cannot
be restored.
Manual backup file The manual backup allows for Designate the path for
path selective backup and restoration automatic backup by
of data. Therefore, the path for means of selecting in the
manual backup needs to be “drive” and “directory”
designated, and is preferably lists.
located on other than system
drive.As in the case of system
failure, which requires
re-installation of the system, the
backup data are lost and cannot
be restored.
Change of automatic backup path
1 In the area of automatic backup path, select the “drive” and “directory” for
automatic backup data.
2 Click the “Confirm” button to store the designated path in the configuration
file.
Change of manual backup path
1 In the area of manual backup path, select the “drive” and “directory” for
manual backup data.
2 Click the “Confirm” button to store the designated path in the configuration
file.
Restoration of automatic backup data
- 29 -
1 Click “Restore automatic backup data”, and confirm the restoration. Then the
backed-up data will be restored into the existing system. The progress is
indicated on the progress bar during the restoration.
Manual backup
1 Select the “Backup Start Date” and “Backup End Date” in the Manual
Backup Timeframe.
2 Click “Manual backup data”, and confirm the backup. Then data during the
designated timeframe will be backed up in the designated path. The progress
is indicated on the progress bar during the backup.
Restoration of manually backup dData
1 Select the “Backup Start Date” and “Backup End Date” in the Manual
Backup Timeframe.
2 Click “Restore manual backup data”, and confirm the restoration. Then the
backed-up data during the designated timeframe will be restored into the
existing system.
The progress is indicated on the progress bar during the restoration.
6.2 General setting

6.2.1 Setting of hospital information
It includes hospital information, department information and doctor information, which are described below in
detail:
Hospital information
- 30 -
Hospital name Name of the hospital Type in the square frame
Director Name of hospital director Type in the square frame
Phone.: Telephone number of the Type in the square frame

hospital
Website： Website of the hospital Type in the square frame
Total Staff The total number of the hospital Type in the square frame
staff
Total Departments The total number of hospital Type in the square frame
departments
Remarks Explanations and descriptions of Type in the square frame

the hospital
Change of hospital information
1 Click “Change”;
- 31 -
2 Input the needed information in the square frame of each parameter;
3 To save the information, click “Save”. Or else, click “Cancel”.
Department information
No. No. of the department Type in the square frame
Name Name of the department Type in the square frame
Manager Name of the manager Type in the square frame
Total staff Total number of department staff Type in the square frame
Phone.: Telephone number of the Type in the square frame

department

the department
- 32 -
Addition of department information
1 Click “Add”;
3 Click “Save”.
Change of the department iInformation
1 Select in the department list the department of which the information is to be

changed
2 Click “Add”;
4 Click “Yes” to delete the department. Or else, click “No”.
Deletion of department information
1 Select in the department list the department to be deleted
2 Click the “Delete” button;
3 Click “Yes” to delete the department. Or else, click “No”.
Doctor information
- 33 -
Department Select the department needed Select from the

drop-down list
No. Doctor No. Type in the square frame
Name Name of the doctor Type in the square frame
Title Position of the doctor Type in the square frame
Phone.: Telephone number of the doctor Type in the square frame
Email Email of the doctor Type in the square frame
Address Address of the doctor Type in the square frame

the doctor
Addition of Ddoctor information
1 Select the department to which the doctor belongs in the drop-down list;
- 34 -
2 Click “Add”;
4 Click “Save”.
Change of Doctor Information
2 Select in the doctor list the doctor of which the information is to be changed;
3 Click “Add”;
5 To save the information, click “Save”. Or else, click “Cancel”.
Deletion of Doctor Information
2 Select in the doctor list the doctor to be deleted;
3 Click “Add”;
4 Click “Yes” to delete the doctor. Or else, click “No”.
6.2.2 User permission management

It includes user management and user permission setting, which are described below:
User management
- 35 -
Login name Name used to log in Type in the square frame
Name Name of the user Type in the square frame
Dept. Dept. of the user Type in the square frame
Phone: Telephone number of the user Type in the square frame
Password Password used to log in Type in the square frame
Confirm password Confirmation of the password Type in the square frame

the user
Addition of user information
1 Click “Add”;
3 Click “Save”;
Change of user Iinformation
1 Click the “〉〉” or “〈〈” button till the user name to be changed is shown on
the interface;
2 Click “Add”;
4 Click “Save” to save the information. Or else, click “Cancel”;
Deletion of User Information
1 Click “〉〉” or “〈〈” till the user name to be deleted is shown on the interface;
2 Click “Add”;
3 Click “Yes” to delete the doctor. Or else, click “No”;
User permission setting
- 36 -
Login in name Name used to log in Click “〉〉” or “〈

〈” till it is shown in the
square frame
Name Name of the user Click “〉〉” or “〈

〈” till it is shown in the
square frame
User permission setting
1 Click “〉〉” or “〈〈”till the user name to which permissions are to be set is
shown on the interface;
2 Set the operational permissions in the general setting group. Click to select,
and click again to de-select;
3 Click bookmarks of all setting groups to set permissions;
4 Click “Save” to save the setting. Or else, click “Cancel”;
- 37 -
6.2.3 Change of user password
Parameters Meaning Operation
User name Name used to log in Choose a user name from the
dropdown menu.
Old password: Old password Type in the square frame
New password: New password to be changed Type in the square frame
New password Reconfirmation to the desired Type in the square frame

confirmation: new password
Change user password
1 Choose the expected new password from the dropdown menu；
2 Input the original registration password in the original password input box；
3 Enter in the new password in the new password input box；
4 Input the new password again in the confirmation new password input box；
5 If you wouldlike to save the new password，click “Enter”；otherwise，click

“backspace”；
6.2.4 Change user registration
- 38 -
User name Name used to log in Choose a user name in the

dropdown menu.
Password Choose corresponding Type in the square frame

registration password to the
user.
Change the login user
1 Choose the new user name which you would like to switch to from the
dropdown menu ；
2 Input the registration user password in the user password input box；
3 If you woule like to switch to the new user，click “Enter”；otherwise，click

“backspace”；
6.2.5 Item print sequence

Set each item，including calculation for its print sequence in the report.
- 39 -
Move forward the item print sequence
1 Select the item to be moved；
2 Click “Move Forward”，then print sequence of the item will move forward 1
bit, click“move forward”continuously ， until the item reach its set print
sequence；
3 Click “Enter”, the setting will become effective; otherwise click

“Backspace”.
Move backward the print sequence of the item
- 40 -
2 Click “Move backward” ， then the print sequence of the item will move
backward for 1 bit, click“backward” continuously, until the item reach its set
print sequence；

“Backspace”.
6.2.6 Item unit
Unit name Unit name of the item Type in the square frame
Description Basic explanation and Type in the square frame

interpretation for the item unit
Add item unit
1 Click “Add”;
2 Input corresponding information in the square frame；
3 Click “Save”.
- 41 -
Change item unit
1 Choose the Item unit you would like to amend in the item unit list；
2 Click “Add”;
4 If you would like to save the change ， click “ save” ； otherwise ， click
“backspace”；
Delete Item unit
1 Choose the item unit you would like to delete in the item unit list；
2 Click “Add”;
3 If you would like to delete the selected item unit，click “Yes”；otherwise，click

“No”；
6.2.7 Item test sequence

Set the test sequence for each item，change of the item test sequence can avoid cross pollution.
- 42 -
Move forward the item test sequence
2 Click “ move forward” button, then the item test sequence move forward for
1 place, click “Move forward” continuously until the item reach its set test
sequence；

“Backspace”.
Move backward the item test sequence
2 Click “move backward”, then the item test sequence move backward for 1
bit, click “Move backward” continuously until the item reach its set test
sequence；

“Backspace”.
6.2.8 Data export configuration

Set the test result data export form and data array layout.
- 43 -
Select export Three kinds of export form for User can choose one export form
form the test result data ： in among three forms as needed.
clipboard, 、 TXT file, EXCEL
file.
Exportable The data item list which can be Select one Data item in the “item
Item exported for normal sample can be exported”，configure it to
test result data， the export item list with the
middle button“>>”.
Derived date For normal sample test result Choose one data item in
item and data ， which has been the“exported item data”list, and
order configured to the data item list you can cancel this exported item
need to be exported, with middle button “<<”, you can
also change the sequence of the
data item with “move upward”
and “move downward” button in
the middle.
Configure the export method, export data item and exported array sequence.
1 User can choose one export form among three forms as needed.
2 Select one Data item in the “item can be exported”，configure it to the export
item list with the middle button“>>”.
3 Select one data item in the “export item data”list and you can cancel this
export item with middle button“<<”.
4 The data item export sequence can be changed by “move upward” and “move
downward” buttons.
5 After selection for above configuration item is finished ， click“ Enter” to

confirm the configuration as backup; otherwise ， click “backspace” to
“ cancel” and remain it is；
- 44 -
6.3 Test setting-up

6.3.1 Item parameters
Item No. Item No. System automatically generated, can not be

changed
Abbr. Item English abbreviation Type in the square frame
Full name Item full Chinese name Type in the square frame
Decimal digits The reserved decimal digits for Dropdown frame，and select from 0,1,2,3.
the test result
Result unit Test result unit Select from the dropdown frame
ItemUnit Set item unit online. The item unit set interface popup after clicking.
settings
Test method Set up the item test method dropdown frame, select from “destination
method”, “two points method”, and “ dynamics
method”.
Direction The change direction of Dropdown frame, select from ascend and
absorbance during reaction. descend.
Wavelength Tested main Wave length dropdown frame ， select from

measured 340,405,450,510,546,578,630,670
- 45 -
Reference Tested secondary wave length Dropdown frame ， Select from the remained
wave length wave length.
Sample volume The sample volume for normal Enter in to the square frame directly ， range is
testing, the unit is microlitre. 3-30，0.1 increase progressively
More Set up the added sample quantity New interface popup after clicking，enter in to
for different type sample the square frame directly.
concentration and dilute test.
R1 volume The 1st reagent quantity when Enter in to the square frame directly. The range
testing，the unit is microlitre. is 150-300，1 increase progressively .
R2 volume The 2nd reagent quantity when Enter in to the square frame directly. The range
testing，the unit is microlitre. is 20-150，1 increase progressively .
Reaction time The starting metering point for Enter in to both adjacent square frame directly,
calculation the numerical value in first frame is ， L，the
numerical value in second frame is M，
So L and M have to meet below relational
expression：
(1) Single reagent Destination method 1≤L ＜
13≤M
(2) Double Reagent destination method 14≤L
＜26≤M
(3) Single reagent” “Two points method” and
“Dynamics method”
14≤L＜M
(4) Double reagent “Two points method” and
“Dynamics method”
35≤L＜M
Display after clicking：
Help Display the purpose of each
metering point and time interval
online.
Calibration Set up item calibration mode Dropdown frame ， select from linear,Log-4P,
mode Log-5P,Exponentional-5P 、 Polynominal-5P ，
spline and factors.If you select “ factors”, then
enter in the specific numerical value in the
square frame.
Detailed setting If the calibration method is not” New interface popup after clicking：
factors”, then the corresponding
button will show up, and the
detailed parameters of the
Enter in the specific numerical value to the input
calibration curve can be set after
- 46 -

clicking. box.
Slope Calibrate the result according to Enter in the specific numerical value to the
formula y=ax+b, therein x is the square frame, default is 1.
actual measured result ， y is the
Intercept calibrated result，a is the slope in Enter in specific numerical value to the square
the calibration formula,b is the frame, default is 0.
intercept in the calibration
formula.
Linearity limit Decide the reaction curve Enter in numerical value between 0-100.
linearity degree.
Exhaust limit The set decision limit for Enter in numerical value between 0-40000.
substrate exhausted during
reaction ， is absorbance value
times 10000.
Prozone check Set up whether proceed front belt radio ， selected means proceed front belt
examination or not, and the time examination；
starting point and examination
limit； The next three frames are activated after it is
selected, the first frame means the front
Front belt examination can only examination time starting point, whose value is
be proceeded with “destination N; the second frame means the front
method”； examination time stop point, whose value is P;
the third frame means the front examination
limit, whose value is PC.N&P and reaction
time L&M have to meet below relational
expression：
Single reagent “Destination method” 1≤L ＜
13≤N＜P＜M
Double Reagent “Destination method” 14≤L ＜
26≤N＜P＜M
PC is integer between 0-100.
Reference Enter in the default normal Enter in specific numerical value in both
range reference range adjacent square frames.
Detailed After clicking, enter in different New Interface popup after clicking：
gender and differnt type sample,
and normal reference range of
different age group. Newly added reference range
(1) Select the gender and sample type from
the dropdown frame；
(2) Click the age upper and lower limit for
the age and reference range；
(3) Click button “save”.
- 47 -

Change reference range
(1) Select the reference range to be changed
in the reference range；
(2) Click “change” for the age upper and
lower limit and reference range；
Delete reference range
(1) Select the reference range to be deleted
from the reference range；
(2) Click “Delete”；
(3) Click “Yes”.
Linearity range Input the item linear range Input the specific numerical value in both
adjacent square frame .
Reaction Input the amplitude range of the Inputthe specific numerical value in both
amplitude range item adjacent square frame, range is 0 to 40000
R1 absorbance Input the normal range of the 1st Input the specific numerical value in both
Range reagent absorbance. adjacent square frame, range is 0-40000
Working Input the normal range of the Input the specific numerical value in both
solution absorbance. adjacent square frame, range is 0-40000
absorbance
range
Add item parameter
1 Click “Add”;
2 Input or select related information to each parameter.
3 Click “Save” to save the items added otherwise click "Cancel”.
Parameters of items corrected
1 Click the item to be corrected.
2 Click “Correction”；
parameters of items deleted
1 Click the item to be corrected.；
2 Click”Delete”；
- 48 -
3 To delete the item, click "Yes" , Otherwise, click "No".
6.3.2 Calibration settings
Parameter Meaning Operation
Name Input the name of the calibration Directly input in the square frame
solution.
Position Set the position of the calibration Click the related position on the left calibration
solution on the calibration panel. panel.
Item Set the concentration of each item Directly input in the square frame
Concentration of the calibration solution.
(2) Basic operation sequence
Adding a calibration solution
1 Click”New addition”；
2 Input related information in each aquare frame.
Correcting a calibration solution
1 Click the calibration solution to be corrected.
2 Click “Correction”；
3 Input related information in each aquare frame. To change the position of the calibration
solution, click the target position (it must be empty), and click "Save".
- 49 -
Deleting a calibration solution
1 Click the calibration solution to be deleted.
2 Click “Delete”；
3 Click "Delete" to delete the calibration solution. If you want to delete it, click "Yes"; otherwise,
click "No".
6.3.3 Quality control(QC) settings
Input the name of the QC

QC name Type in the square frame
solution.
Set the target value of each

Item target value Type in the square frame
item of the QC solution.
Set the standard deviation of

Item SD Directly input in the square frame.
each item of the QC solution.
Cumulative sum Set the item accumulation and

Select an option from the drop-down list box.
control rules control rule.
Adding a QC solution
1 Click “Add”;
- 50 -
3 To save the added QC solution, click "Save". Otherwise, click "Cancel".
Correcting a QC solution
1 Click the QC solution to be corrected.
4 To save the correction, click "Save". Otherwise, click "Cancel".
Deleting a QC solution
1 Click the QC solution to be deleted.
3 To delete the QC solution, click "Yes". Otherwise, click "No".
6.3.4 Item calculation
Abbr. The English abbreviation of the Type in the square frame

item calculated.
Full name The full Chinese name of the item Type in the square frame
calculated.
Result unit Calculate the result unit of the Select an option from the drop-down list frame.
- 51 -

item. You can also select none.
Decimal Keep decimal of the result of the Dropdown frame，and select from 0,1,2,3.
item calculated.
Reference Input the default reference range. Enter in specific numerical value in both
range adjacent square frames.
Detailed After clicking, enter in different New interface popup after clicking：
gender and differnt type sample,
and normal reference range of
different age group. Newly added reference range
(1) Select the gender and sample type from
the dropdown frame；
(2) Click the age upper and lower limit for
the age and reference range；
Correction Reference Range
(1) Select the reference range to be changed
in the reference range；
(2) Click “change” for the age upper and
lower limit and reference range；
Delete reference range
(1) Select the reference range to be deleted
from the reference range；
(2) Click “Delete”；
(3) To delete the reference range, click "Yes".
Otherwise, click "No".
Adding a calculation item
1 Click “Add”;
3 In the item selection zone, click the item to be selected. In the calculation button zone, click a
value and a calculation symbol to compose a formula for calculating the item.
4 To save the added calculation item, click "Save". Otherwise, click "Cancel".
Correcting a calculation item
1 In the calculation item list display zone, select the calculation item to be corrected.
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4 To modify the calculation formula, click "Rebuild Expression", and input a new expression.
5 To save the correction, click “Save”. Otherwise, click “Cancel”.
Deleting a calculation item
1 In the calculation item list display zone, select the calculation item to be deleted.
3 To delete the calculation item, click "Yes". Otherwise, click "No".
6.3.5 Item combination
No. Number of the item combination System automatically generated, can not be
changed
Abbr. English abbreviation of the item Type in the square frame

combination
Full name The full Chinese name of the item Type in the square frame
calculated.
Adding an item combination
1 Click “Add”;
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2 In each square frame, input related information. In the item selection zone, click an item. The
item is selected by one click, and it is deselected by another click.
3 To save the added item combination, click the "Save". Otherwise, click "Cancel".
Correcting an item combination
1 In the item combination list display zone, select the item combination to be corrected.
3 In each square frame, input related information. In the item selection zone, select or deselect an
item.
4 To save the correction, click "Save". Otherwise, click "Cancel".
Deleting an item combination
1 In the item combination list display zone, select an item combination to be deleted.
3 To delete the item combination, click "Yes". Otherwise, click "No".
6.3.6 Sample type settings

The following section describes sample type settings in detail.
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Type Sample type name Type in the square frame
Remarks: Basic description or explanation Type in the square frame

of the type
Sample type A list of existing sample types You can select a sample type
list record in the list, and correct or
delete it as required.
Adding a sample type
1 Click “Add”;
3 Click “Save”;
Correcting a sample type
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1 In the sample type details list, select a sample type to be corrected.
3 Edit and modify related information in the square frame.
4 If you would like to save the change ， click “ save” ； otherwise ， click
“backspace”.
Deleting a sample type
1 In the sample type details list, select a sample type to be deleted.
3 If you would like to delete the selected item unit，click “Yes”；otherwise，click

“No”；
6.4 Statistics/Query
6.4.1 History Query
Query the history test result of a regular sample.
Test date Start date of the samples to In the square frame, directly input a date, or
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be queried select one from the drop-down list box.
Sample No. Start code of the samples Type in the square frame
to be queried
Conc. Concentration range of the Type in the square frame

samples to be queried
Name Patient name of the Type in the square frame

Sex Patient gender of the Select an option from the drop-down list box.
Age Patient age of the samples Type in the square frame

to be queried
Age unit Age unit of the samples to Select an option from the drop-down list box.
be queried
Patient No. Out-patient code of the Type in the square frame

Admission In-patient code of the Type in the square frame

No. samples to be queried
Bed No. Hospital bed code of the Type in the square frame
Submitted Submitting physicians of Type in the square frame

Doc. the samples to be queried
Docimaster Inspection doctor of the Select an option from the drop-down list box.
Sample ID ID of the samples to be Type in the square frame

queried
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1 In the query conditional input zone, input or select one or more query conditions.
2 In the query item selection zone, select one or more items.
3 Click “query”.
4 In the query result display zone, all the records meeting the query conditions are
displayed.
5 Select a query record, and click “Reaction curve”. You can see the real-time reaction
curve of this record.
6 If you only query one item, the “Profile” button is active. You can click it to view
the distribution of all query records.
7 Click “Recalculation”, the software will automatically recalculate and display all the
results in the query result list.
8 Click “Export”, and zccording to the configuration export method and format
mentioned in Section 9.2.8, the system will export all the results in the query result
list.
9 Click “Return” to return to the main operation interface.
6.4.2 Quality control(QC) query

Query the QC status of each item and the QC chart, shown as below:
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Viewing QC status
1 In the QC type selection zone on the left, select a desired QC type from real-time QC, intra-day QC,
and inter-day QC.
2 Select a date period and item name.
3 Click “query”, in the QC solution display zone, all the used QC solutions are displayed. In the QC
status display zone, the QC status of the item is displayed.
4 Click “Detailed QC Result”, the test results of each QC solution within the selected time period are
displayed
5 Click “Multi-rule QC Chart”, “Accumulated QC Chart”, and “twin plot” , the related QC charts of
the item within the selected time period are respectively displayed.
6 Click “Print” to print the related QC charts and QC data.
7 Click “Curve to Print” to print the related QC charts.
8 Click “Return” to close the QC status interface and return to the main interface.
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6.4.3 Calibration query
Basic operation sequence
Querying the calibration records of an item
1 In the Calibration Date bar, select the start date to be queried.
2 In the query item selection bar, select an item to be queried. The item is selected by one click, and
it is deselected by another click.
3 Click “query”, in the query result display zone on the right, all the calibration records meeting the
query conditions will be displayed.
4 In the query result display zone, select a calibration record.
5 Click “Details” to display a new interface, where the detailed information about the calibration
record is displayed.
6 To print the details of a calibration record, select it, and click "Print".
7 To set a calibration record as the current record, select it and click “Set to Current”. Then, the
concentration values of later tested samples of this item will be calculated according to the current
calibration record.
Viewing detailed calibration information
1 In the query result display list, select a calibration record.
2 Click “Details” button to display the detailed information about the calibration, shown as below:
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6.4.4 Working volume statistics
Operator Operator of working volume statistics Select from the drop-down list
Sample type Sample type of working volume statistics Select from the drop-down list
Time Time of working volume statistics Click an option to select it, or directly
input a value.
All items If selected, it indicates the number of all The option is selected by one click, and
items in the statistics. it is deselected by another click.
Working Volume Statistic
1 From the drop-down list box, select an operator and a sample type.
2 Select or input a working volume statistics period.
3 To query all items, click the “All Items” radio. Otherwise, select an item in the query item
selection zone.
4 Click “query”.
5 In the query result display zone on the right, all the results meeting the query conditions are
displayed.
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6.5 Maintenance
6.5.1 Instrument Debugging and Maintenance
The maintenance scope includes the main control unit, sampling probe unit, stirring rod unit, reaction tray
photoelectric unit, and reagent sample tray unit. They are respectively described as follows:
 Main control unit (MCU)
Maintenance action interpretation
Handshake instruction Communication handshaking between the PC and each unit chip
System distribution Step-by-step reset of each movement component

self-check (initialization)
1 Select a command on the left, and click "Send".
2 To send cyclically, select "Send Cyclically", and input the interval and number of times in the
subsequent square frames. Then click "Send".
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 3-R1 unit

Handshake instruction Communication handshaking between the PC and the MCU/sample
probe stirring rod unit chip.
R1 Probe Vertical reset The sampling probe in vertical direction, reagent injector, and sample
injector return to the init bit in vertical direction.
R1 Syringe Vertical reset The Syringe returns to the init bit (cleaning bit) in horizontal direction
(init bit - sample drawing bit in the outer ring - sample drawing bit in
the inner ring - sample loading bit of the reaction plate - cleaning bit).
R1 Probe Rotating Reset The sampling probe rotates horizontally to the init bit (cleaning bit).
R1Tray Rotating Reset The sampling probe rotates horizontally to the sample loading bit of
the reaction tray.
R1 Tray Totate to certain Pos. The sampling probe rotates horizontally to the cleaning bit (init bit).
R1 Probe Rotate to R1 Tray The sampling probe rotates horizontally to the sample drawing bit in
the outer ring of the sample reagent plate.
R1 Probe Rotate to Reaction The sampling probe rotates horizontally to the sample drawing bit in
Tray the inner ring of the sample reagent plate.
R1 Probe Rotate to cleaning The sampling probe moves to the init bit in vertical direction.
station
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Move downward to init bit The sampling probe moves downwards to the init bit.
Downward movement to the The sampling probe moves downwards to the cleaning bit.
cleaning position
Down to the reagent bottle or The sampling probe moves downwards to the reagent bottle or sample
sample cup cup, and stops until it detects the liquid level.
Sampling probe dips into The sampling probe moves downward into the reaction vessel, and
reaction vessel moves a specific number of steps.
Move downward a specific The sampling probe moves downward a specific number of steps.
number of steps
Move downward to draw The sampling probe moves downward, draws a sample, and then
sample (and back again after move upwards again.
drawing)
Move downward to reaction The sampling probe moves downward, emit a sample, and then move
vessel to emit sample upwards again.
Down to cleaning pool for The sampling probe dips into the cleaning pool for cleaning.
cleaning
Reagent residue check The sampling probe checks the remaining volume of the reagent.
command
Injector to init bit The injector moves to the initial position.
Injector draws sample The sample injector draws a sample of a preset volume.
Injector emits sample The sample injector emits a sample of a preset volume.
Always open sampling probe Open the sampling probe external wall valve.
external wall valve
Close sampling probe external Close sampling probe external wall valve
wall valve
Open/Close sampling probe Open or close the sampling probe external wall valve at a given time.
external wall valve by time
Always open sampling probe Open the sampling probe inner wall valve.
inner wall valve
Close sampling probe inner Close sampling probe inner wall valve
wall valve
Open/Close sampling probe Open or close the sampling probe inner wall valve at a given time.
inner wall valve by time

1 Select a command on the left, and click “Send”.
2 To send cyclically, select “Send Cyclically”, and input the interval and number of times in the
subsequent square frames. Then click “Send”.
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4-R2 unit
Handshake Communication handshaking between the PC and the MCU/sample

probe stirring rod unit chip.
R2 Probe vertical reset The stirring rod returns to the init bit in vertical direction.
R2 syringe vertical reset The stirring rod returns to the init bit in horizontal direction (init bit –
stirring bit of the reaction plate - cleaning bit)
R2 probe rotating reset The stirring rod moves to the init bit in rotation direction.
R2 tray rotating reset The stirring rod rotates to the position of the stirring rod cleaning pool.
R2 tray rotate to certain R2 The stirring rod rotates to the upper stirring bit of the reaction plate, and
Pos. dips a specific number of steps to the cuvette.
R2 probe rotate to R2 tray The stirring rod moves vertically to the init bit.
R2 probe rotate to reaction The stirring rod moves downwards to the position of the cleaning pool.
tray
R2 probe rotate to cleaning The stirring rod moves downwards into the reaction vessel.
sation
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R2 probe down to cleaning The stirring rod moves downwards a specific number of steps.
station
R2 probe down to reagent The stirring rod moves downwards into the reaction vessel (and back
bottle again after stirring).
Dip to the cleaning pool The stirring rod dips to the position of the cleaning pool (and back again
(and back again after after cleaning).
cleaning)
Always enable the stirring Open the stirring rod motor to let the stirring rod stir.
command
Disable the stirring Close the stirring rod motor to stop the stirring rod.
command
Enable/Disable stirring by Open the stirring rod motor and set the stirring time.
time
Always open the stirring rod Open the stirring rod external wall valve, and clean the external wall of
external wall valve the stirring rod.
Close the stirring rod Close the stirring rod external wall valve, and stop cleaning the external
external wall valve wall of the stirring rod.
Open/Close the stirring rod Open the stirring rod motor, and set the time for cleaning the external
external wall valve by time wall of the stirring rod.
 Reaction Tray photoelectric unit
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Handshaking Communication handshaking between the PC and the MCU/reaction

tray photoelectric unit chip.
Rotation reset The reaction tray goes through the init bit in a direction and stops at the
first cuvette.
Reaction tray rotates through The reaction tray rotates through the init bit to specific cup position.
init bit to specific cup position
Reaction disk directly rotation The reaction tray rotates from the current position directly to the
to the specified cup position specified cup position.
Reaction disk rotation to the The reaction tray rotates from the current position directly to the static
static collection cup position collection cup position.
Rotate the reaction disk directly Rotate the reaction disk directly
Stop the reaction disk in the Stop the reaction disk in the direct rotation
direct rotation
Static photo-electricity The reaction tray does not rotate, but collect the photoelectric data of the
collection command preset wavelength.
Liquid open pump Open the liquid pump, and maintain the preset time.
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Liquid close pump Close the liquid pump.
Open liquid refilling pump Open the liquid refilling pump to refill.
Close liquid refilling pump Close the liquid refilling pump to stop refilling.
 5-S unit

Handshake instruction Communication handshaking between the PC and the MCU/reaction
tray photoelectric unit chip.
Reagent sample tray rotates to reset The reagent sample tray goes through init bit and stops at bit 1.
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Reagent tray rotates to specified cup Reagent tray rotates to specified cup position through init bit
position through init bit
Reagent tray rotates directly to The reagent tray rotates from the current position directly to the
specified cup position specified cup position.
Sample tray rotates to specified cup Sample tray rotates to specified cup position through init bit
position through init bit
Sample tray rotates directly to The sample tray rotates from the current position directly to the
specified cup position specified cup position.
Directly rotate reagent sample disk Directly rotate reagent sample disk motor
motor
Directly stop rotating reagent sample Directly stop rotating reagent sample disk motor
disk motor

6.5.2 Cuvette Maintenance (This function is applicable only to the instrument system with
self-cleaning function)
According to maintenance requirements, conduct a thorough cleaning on all cuvettes. Click the menu to
display the following interface:
Clean all cuvettes: Clean all cups on the cuvette tray one by one.
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Wipe all cuvettes: Evacuate and wipe all cups on the cuvette tray one by one.
Fill all cuvettes with water: Fill water in all cups on the cuvette. Starting from cup No.1, fill water in five cups
as a group. Perform the operation 14 times. At last, there are still two empty cups. If necessary, you can
manually fill the two cups with water.
For the three functions including "Clean all cuvettes, wipe all cuvettes, and fill all cuvettes with water", you
can select one or more of them as required. Then click the "Start" button for corresponding processing.
First, select the contents of maintenance as required. Then click the "Start" button. The instrument will start
maintaining the corresponding cuvettes. The process is in this order: clean all cuvettes, wipe all cuvettes, and
fill all cuvettes with water. The process is over until the last maintenance item is completed. If you do not want
to maintain cuvettes, click "Return".
6.5.3 Clearing Samples of Non-result

From the current sample records, clear the samples that are requested but not tested yes. Click the menu to
display the following interface:
Click the "Y" to start clearing the corresponding records. Click the "N" to abort the operation and return to the
main interface.
6.5.4 Transferring Sample Test Data to the History Database

Transfer the current sample record to the history database. Click it to display the following interface:
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Click the "Y" to start transferring the corresponding record. Click the "N" to abort the operation and return to
the main interface.
6.5.5 Querying operation Error Logs

According to the inputted query conditions, query the related error information saved in the database. Click it
to display the following interface:
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Start Date Start date of the error In the square frame, directly input a date, or select one
information to be queried from the drop-down list box.
End Date End date of the error In the square frame, directly input a date, or select one
information to be queried from the drop-down list box.
Query error List of error information Move the mouse to a record in the list. The related
information list satisfying the query period information will be displayed.
Detailed Detailed description of an

description for error selected from the error
selecting error information list
The possible cause
Possible causes of an error
for seclecting error
selected from the error
information list
Selected Suggested Suggested handling method

Processing of an error selected from the
Approach to the error information list
Error
Querying error information
1 In the query condition input zone, input the start/end time of errors.
3 Click “query”. If any error information satisfies the query conditions, it will be written into
the "Error Information List".
Delete Current Error Information
1 In the "Error Information List, select unwanted error information.
2 Click “Delete Current Error Information”. After confirmation, delete the selected error
information.
Clear All Error Information
1 Click “Clear All Error Information”. After confirmation, delete all the error information in
the database.
6.5.6 Whether to Store Operation Error Information In Database

Click "Whether to Store Operation Error Information In Database" menu. If the menu is checked, error
information is stored in the database. Otherwise, error information is not stored in the database. When the
menu is clicked, the menu shifts from checked (or unchecked) status to unchecked (or checked) status.
6.5.7 Communication connection (This function is applicable only to the instrument

system with self-cleaning function)
This function realizes communication between the upper computer and lower computer. When the upper
computer software does not quit, and the instrument is powered off and then powered on again, it's not
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necessary to stop the upper computer software. By implementing the "communication connection" function,
you can set up a communication link between the upper computer and lower computer.
6.6 Shortcut buttons

Shortcut buttons are distributed on the main interface, as shown below:
6.6.1 Calibration Request

In the left toolbar on the main interface click “Calibration Request” to display the following functional
window:
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Calibration test request
1 In the calibration item selection zone on the left, select an item. The item is selected by one click,
and it is deselected by another click.
2 In the item calibration solution request zone, select one or more calibration solutions according to
the hints on calibration types shown below.
3 If you want to perform calibration on multiple items, repeat steps 1 and 2, until all the items are
selected.
4 Click "Request".
5 Click “Calibration Request List”. You can see the detailed calibration request list.
6.6.2 Test Request

In the left toolbar on the main interface click “Test Request” to display the following functional window:
Request testing regular and QC samples. The operation interface is as shown below:
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Sample Code of the tested sample Type in the square frame

code
Batch input Input samples of the same test in Input the number of samples inputted in batches.
batches
Sample Select a sample plate code. There Select an option from the drop-down list box.
plate code are four plates in all.
Cup Cup position in a sample plate. Input a value in the square frame, or click "Remaining
position There are 24 positions in all. cups view" and select a cup position from the pop-up
box.
Remaining View the empty positions on the Click this option to display a new interface. Select an
cups view current sample plate. empty cup position, and click "OK".
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Sample Type of the selected sample Select an option from the drop-down list box.
type
Emergency Set the current sample as Radio. If selected, it indicates emergency treatment.
treatment emergency treatment
Apply for a single sample
1 Input sample No;
2 Select sample disk no., cup position no.and sample type;
3 If emergency treatment test, please select Eemergency treatment radio, otherwise do not select;
4 In Item test selection area, click the items needed to be tested, with clicking one time for selected,
and one time again for cancel. Or in the combination item selection area, click the combination
set up, with clicking one time for selected, and one time again for cancel. If you need to set up a
combination item online, click “Combination Setup” and setup the combination on the new
interface popped up, and then return.
5 Just click “Apply for”.
Batch samples application
1 Input the initial sample No, then input the number of batch;
2 Select sample disk no., cup position no.and sample type;
3 In Item test selection area, click the items needed to be tested, with clicking one time
for selected, and one time again for cancel. Or in the combination item selection area,
click the combination set up, with clicking one time for selected, and one time again
for cancel. If you need to set up a combination item online, click “Combination
Setup” and setup the combination on the new interface popped up, and then return.
4 Click "Request".
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Note:
(1) When batch application, the number and the position in sample disk of the sample
demanding batch application should be increased progressively with order based on
the number and cup position of the initial sample.
(2) Batch application and a single application can be done at the same time.
Check the sample applied
1
By means of clicking the button or , the application information of the first or the
last number can be viewed; By means of clicking the button or , the application
information of the prior or the latter number can be viewed;
Correct the application of a sample
1
Click the button , , or into the number of samples intended to be
modified;
2 In item test selection area, click the items needed to be tested, with clicking one time for selected,
and one time again for cancel.;
3 Click "Request".
4 For samples that have been applied but not tested, if you want to delete the extra application
items, please move the mouse below the item and press the right key, and then a new interface
will pop up and you just press the “Delete” button. If you want to add the omitted items, please
directly click the item and then just press “Apply for” button. But the samples that have begun to
test cannot be modified.
Note:
Only the number of ordinary and quality control test that have been applied but not started
can be viewed and modified.
6.6.3 Start
On the left toolbar of the main interface, please click “Start” and then the following functional form that starts
test will appear. Select the type and range that intended to start test, and start test after clicking “OK”(see
below picture):
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Start test
1 “Cup state” indicates that the service condition of each cup in the reaction disk can be viewed.
When there are no used cups (dirty cups) in the reaction disk, click “Cup change confirm” to tell
the operating system that all cups in the reaction disk are clean. After starting test, the operating
system will start test from the first cup.
2 Select the number (1-5) of reagent disks and the number (1-4) of sample disk that intended to
start test;
3 If you need the reagent blank can be tested automatically, please tick in the box in front of
“Automatically test reagent blank”, otherwise directly enter into next step.
4 If you do not select “Automatically test reagent blank” and want to test the reagent blank of
individual item, click “Reagent Blank” and select the reagent intended to test, then click “OK”
(clicking one time for selected, and one time again for cancel);
5 In the system, the calibration test is independent. That is, if you want to conduct the calibration
test, the calibration test must be completed, then other non-calibration test can be started.
Certainly, during the calibration test, the reagent blank test also can be conducted at the same
time. In the system, the test sequence is: reagent blank test – calibration test—quality control test
– ordinary test. Prior to starting any test, tests related to reagent blank can be added.
5 If the test for this batch includes the quality control test, please click “Quality Control” (clicking
one time for selected, and one time again for cancel).
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6 Click “Ordinary Test” (clicking one time for selected, and one time again for cancel), and input
the starting number of ordinary test intended to start in the below input box. If you do not input
anything, it will be defaulted to start all ordinary tests.
7 Just click “OK” button.
Note:
SL 120 system can start the reagent blank test, quality control test and ordinary test at the
same time based on the below priority level.
(1) Reagent blank test
(2) Quality control(QC) test
(3) Ordinary test
Note:
(1) For the reagent blank test, calibration test and quality control test, once they are
selected, the test of all current applied items mentioned above will be started.
(2) For the ordinary test, you can randomly choose the sample number that intended to
start test. If only the starting sample number is input, all tests after the number will
be started; If only the closing sample number is input, all tests before the number
will be started; if no sample number is input, all tests that have applied will be
started.
6.6.4 Check the Result

On the left toolbar of the main interface, please click “Result View” and then the below form will appear. View
all ordinary tests status and test results (see below form):
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(1) Basic Interpretation of the parameters
Name Name of the current patient Type in the square frame
Sex Sex of the current patient Select an option from the drop-down list box.
Age Age of the current patient The first box can be input directly, and the second box can be
selected from the drop-down menu.
Patient Patient No. of the current Type in the square frame

number patient
Admission Admission No. of the current Type in the square frame

number(AD) patient
Department Department of the current Select an option from the drop-down list box.
of sample patient
sending
Bed No. Bed No. of the current patient Type in the square frame
Clinical Clinical diagnosis for the Chosen from the drop-down menu or input directly
diagnosis current patient
Submitting Doctor who opens the Input the doctor number or input directly
physicians inspection application form for
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the current patient
Inspection Sample inspection time Input or click directly to select

time
Blood Sample collection time Input or click directly to select

sampling
time
Sample type Sample type for inspection Select from the drop-down list
Sample Sample status for inspection Select from the drop-down list
status
Auditor The name of doctor who signs Select an option from the drop-down list box.
audit report form
Notes: Matters needed to be explained Input directly

for the sample and report etc.
Delete Delete the information and Click to pop up a new interface and then click “OK”
singly relevant test items of one
sample under current non-test
status
Also delete all sample data which has finished test
Delete all Delete the information and Click to pop up a new interface and then click “OK”
relevant test items of one
sample under current non-test
status, also can delete sample
data which have finished test
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View current result
1 Select one sample number which needs to be input patient information from the left sample
number list area;
2 All test results of selected numbers can be displayed directly in the results display area in lower
right. If the test of one item has not been finished, the Result Display Area of the item will display
nothing;
3 After selecting one item that has had result, please click “Reaction Curve” to view the reaction
curve and reaction data of the result.
Input Patient Information
1 Select one sample number which needs to be input patient information from the left sample
number list area;
2 Input all relevant information in the Information Input Area in upper right and then click “Save”
on the right;
3 Switch to next sample number, and then repeat step 1 and step 2 to complete the information
input of next number.
Setup Print Template
1 Click “Print Template”, and a new interface pops up;
2 Select the available print template or built a new one, and setup it as default.
Result Print
1 If you want to print all results, please click radio-button “All samples”, then click “Print”;
2 If you want to print the result with its number selected, please click radio-button “Current
Selected Sample”, then click “Print”;
3 If you want to print the results with its number appointed, please click radio-button “Select
Sample”, and input directly the appointed numbers in the activated input box behind, then click
“Print”;
Results re-calculation (when the standard curves of one or some items are changed, it is not need to
retest the items. Only their test results needs to be recomputed.)
1 If one sample on the left of the interface is selected, its test items results will be displayed in the
item results list.
2 If you click ”Re-calculation”, the software will automatically re-calculate the results of all test
items of the selected sample and display the results.
Export (according to the export ranges “current sample, all samples, selected sample” closed by users,
which is the same to the selection of sample print)
1 The sample range exported by selection results: “current sample, all samples, selected sample”,
the sample selection mode is in the interval between the independent sample number separated
with “.” and the consecutive sample number separated with “_” input in the latter box.
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2 If you click “Export”, the system will export the mode and format according to the configuration
in 9.2.8 and complete the appointed sample results export.
6.6.5 Stop
Click “Stop” on the left toolbar of the main interface, the below form will appear. The function of stopping all
tests in progress only can be performed in case that the user needs to stop the current work due to a variety of
reasons.
a After clicking “OK”, the analysis meter will stop immediately. If you do not want to stop, just click
“Return”.
6.6.6 Normal Exit

Click “Normal Exit” on the left toolbar of the main interface, the below form will appear. The normal exit
program only can be performed in case that the analysis meter is on standby.
Directly click “OK”;
6.6.7 Emergency Exit

Click “Emergency Exit” on the left toolbar of the main interface, the below form will appear. The function
only can be performed in case that the analysis meter fails during running and cannot be exited normally.
During emergency exit, the analysis meter will not perform any shutdown process and exit directly.
Directly click “OK”.
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6.6.8 Open/Hidden Menu

Click “Open Menu” or “Hide Menu” on the left toolbar of the main interface. After clicking “Hidden Menu”,
the system will hide the main menu, and be changed as “Open Menu” status at the same time. If click again,
the main menu will appear, and be changed as “Hidden Menu” at the same time.
Before clicking:
Start
Stop
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After clicking:
6.6.9 Sample Disk

Click “Sample Disk” on the lower toolbar of the main interface, the below form will appear. View test status of
the applied samples in all sample disks including the calibration disks as shown below:
(Sample disk status diagram of SL 120 instrument)
(Sample disk Status diagram)
Sample Disk Status View
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1 Switch the bookmark of sample disks to view test status of samples in different sample disks.
2 In the sample disk position display area, different test types and test status will be indicated in
different shapes and colors.
3 After selecting one sample in the sample disks, the sample number and sample type will be
displayed in the right information display area for selected samples, the applied items and the test
status of all items will be displayed in the test results display area, and the time needed for the
sample to finish test will be displayed in the test remaining time estimation column.
4 After selecting one item that has finished test from the test results display area of the selected
samples, click “Select reaction curves of finished items” to view the reaction curve and reaction
data of the test.
5 Click “Return” to close sample disk interface and return to the main interface.
6.6.10 Reagent Disk

Click “Reagent Disk” on the lower toolbar of the main interface, the below form will appear. View and setup
the reagent position and the remaining reagent amount in all reagent disks to ensure that the remaining amount
of all reagents can be tested in standby mode.
(Reagent Disk Status Diagram)
Reagent Disk Status View
1 Switch the bookmark of the reagent disk to view the position and remaining amount of reagents
in different reagent disks
2 In the left reagent disk position display area, the occupation conditions of reagent position will be
displayed in “Occupied” and “Free”.
3 In the right item display area, gray indicates that the reagent position of the item has been setup;
and yellow indicates that the reagent position of the item has not been setup.
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4 In the reagent display area that has been setup, the position and remain of all reagents can be
viewed.
Setup Reagent Position
1 Switch the bookmark to the reagent disk needed;
2 In the right item display area, please click one item in yellow, and also activate R1 or R2 if
dual-reagent, then double click one vacant position in the left reagent disk, thus the reagent can
be setup to the position.
Change Reagent Position
2 Firstly click one reagent needed to change position, then double click one vacant position, thus
the reagent can be setup from original position to the current one.
Delete Reagent Position
2 Firstly click one reagent needed to delete position, then click right “Delete occupied cup
position”, thus the reagent position can be deleted.
Reagent Residue Detection
2 Click “Test Reagent Remain” to popup a new interface. Select the reagent needed to test the
remain and then click button “OK”.
Note:
(1) For the dual-reagent item, its R1 and R2 should be setup in one reagent disk;
(2) Reagent remain test only can be tested in the standby mode.
6.6.11 Reaction Disck

Click “Reaction Disk” on the lower toolbar of the main interface, the below form will appear. View the test
items and test status of all reaction disk shown as below:
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(Reaction Disk Status Diagram)
Reaction disk state view
1 In the left reaction disk position display area, the test status of all reaction cups will be displayed
in different colors.
2 If click one reaction cup, all the items tested and in the progress of test on the same day will be
displayed in the right test item column of the day for the selected reaction cups.
3 If select one test, the sample number and sample type of the item will be displayed in the detailed
information column for the selected test items.
4 If select one finished test item, and click button “Reaction curve of selected finished items”, the
reaction curve and reaction data of the item can be viewed;
5 Click “Return” to close the reaction disk interface and return to the main interface
6.6.12 Alarm Information

Click “Alarm Information” on the lower toolbar of the main interface, the below form will appear. When you
want to view the current alarm information, including specific alarm contents, possible causes of alarm and
solutions etc. the alarm button will be shown in high light red if there are new alarms that has not been viewed.
The red will disappear after viewing. shown as below:
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Click “Return” to close the alarm information interface and return to the main interface. For all previous alarm
information, please see Error Log Query.
6.7Instrument Running Status Diagram

There are three status diagram in the upper right of the main interface as below:
From left to right is expressed as: the first one is “Liquid Waste” (there are two liquid levels for the liquid
waste in the waste container: “Full” and “Not Full”); the second one is “Cleaning Water” (there are four water
levels: “Empty”, “Water Replenishing” and “between Water Replenishing and Full” and “Full”); the third one
is “Instrument Running” (there are two status: standby and running, with the graph in dynamic rotation
indicating the instrument is in testing, with the graph in static indicating the instrument is in standby or not
online).
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Chapter 7 Computing method

7.1 Test process
SL 120 execute fixed test process, each reaction can have N test Cycle, N=“Item destination”(The stop point of
“reaction start-stop part” for “item parameter”) + added test points (can be configured in the configuration files,
default configuration is 6 points) , each cycle takes 20 seconds, as shown in Figure 10-1 as below:
Reaction cycling
Double reagent test
Add 2nd reagent

13 cycles
Max. 49 cycles
Start-up process
Shut down process

Add 1st reagent
Reaction ends
Add samples
About 10 About 3 13 cycles About 5

Start-up
seconds minutes minutes
Shut down
Max. 49 cycles
Single reagent test
Test process
Figure 10-1 Test process schematic diagram
7.2 Metering point

For same reactions, each cycle proceeds one time metering, and totally N metering points (explanation of N is
as above), the interval for two adjacent metering points is 20 seconds, as shown in Figure 10-2:
Explain the metering spot in detail
Absorbance
Join R1 Join S Join R2
Metering spot
Return
Figure 10-2 Metering point schematic diagram
7.3 Analytical method

Classify the reactions according to the reaction speed features during reaction process, SL 120 classify all
reactions into three categories: Destination method. Two points method and Dynamics method, which will be
described as below separately
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7.3.1 Destination method

The reaction complete thoroughly, all analyses has transformed into the monitored product, the reaction liquid
absorbance will not increase (or decrease), and further more the increased (or decreased) quantity of
absorbance before and after reaction is proportional to the original concentration.
Typical destination method reaction curve is as in below figure:
Figure 10-3 Destination method reaction curve
7.3.2 Two points method

In specific reaction part, reaction speed is proportional to first power of analyte concentration, and since the
analyste is being consumed continuously, the whole reaction speed is decreasing continuously, which shows as
the increasing (or decreasing) speed of absorbance is more and more slower, and during this part, the
increasing(or decreasing) quantity of the absorbance is proportional to the original concentration of the
analyte.
Typical two points method reaction curve as shown in Figure 10-4:
Figure 10-4 Two points method reaction curve
7.3.3 Dynamics method

In specific reaction part, the reaction speed reach the maximum and remain unchanged, the analyte generates
the monitored product with the maximum speed at a constant rate, which shows as absorbance decrease or
increase evenly, and decreasing or increasing speed (△A/min) is proportional to the activity or concentration
of the analyte, and mainly used for determination of enzyme activity.
Typical Dynamics method reaction curve as shown in Figure 10-5:
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Figure 10-5 Dynamics method Reaction curve
7.4 Absorbance and reaction amplitude
7.4.1 Absorbance
for SL 120, the computation formula of absorbance is as below:
Solution Absorbance=Log (AD water－AD dark) / (AD solution－AD dark)
Thereinto:
(1) “Log” means normal 10-base logarithm operation;
(2) “AD”means the numerical value of transmitting light intensity after photoelectric conversion and
digitaltoanalog conversion ;
(3) “AD dark” means the AD value with light off, “AD water” means the AD value for reaction vessel
filled up with water, “AD solution” means the Ad value for reaction vessel filled up with solution to be
tested;
7.4.2 Reaction amplitude

For SL 120, reaction amplitude is defined as the change rate of absorbance before and after reaction or during
reaction, reaction amplitude is important middle data for computing process, and is also necessary for
calibration parameters and concentration computing. For different type of analytical method, the computing
method of reaction amplitude is not the same, which will be described as below
(1) Destination method
Reaction amplitude= Destination absorbance－( Starting point absorbance× volume correction factor )
Thereinto:
 Destination and starting point is set in the “computing time” of “item parameter” by the user;
 For absorbance, if it is single wavelength, then use the absorbance of the dominant wavelength; if it is
dual-wavelength, then the absorbance of each point equals to dominant wavelength absorbance minus
sub-wavelength absorbance;
 Volume correction factor = ( Starting point volume) /( Destination volume);
(2) Two point method
Reaction amplitude = Destination absorbance - Starting point absorbance
Thereinto:
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(3) Dynamics method
Reaction amplitude = The change rate per minute for starting point and destination absorbance
Thereinto:
7.5 Calibration
7.5.1 Calibration type

For SL 120, calibration is classified into linear calibration and no-linear calibration. Linear calibration includes
single point calibration, two point calibration and multipoint calibration, which are mainly applied to the item
with solution as the reaction liquid; No-linear calibration mainly includes Logit -4P, Logit -5P, Exponential-5P,
Polynomial-5P and Spline, which are mainly applied to the item with turbid liquid such as immunization turbid
as the reaction liquid.
7.5.2 Calibration parameters

For different calibration type, its calibration parameter number and computing method is not the same, which
will be described as below.
(1) Single point linear calibration
Formula C=R/k, with one calibration parameter, which is k.
k= R calibration/calibration
Thereinto: C calibration is the standard sample concentration, R calibration is standard sample reaction
amplitude.
(2) Two point linear calibration
Formula C=(R-b) /k, with 2 calibration parameters, which are k and b.
k= (R2-R1) / (C2-C1) b= R1- C1 (C2-C1) / (R2-R1)
Thereinto: C1, C2 is the concentration of standard sample1 and 2, R1, R2 is the reaction amplitude standard
sample1and 2.
(3) Multipoint linear calibration
Formula C=(R-b) / k, with 2 calibration parameters, which are k and b.
Calculate the calibration parameter according to multipoint linear regression.
(4) Logit-4P
Calibration formula R=R0+K/[1+e(-a+blnc)], with 4 parameters, which are R0,K,a and b. It is required to provide
at least 4 standard sample, the concentration(activity) of first standard sample is zero, the corresponding R
equals to R0, other parameters can be determined with iteration method.
(5) Logit-5P
Calibration formula R=R0+K/[1+e(-a+blnC+c*C)], with 5 parameters, which are R0,K,a,b and c. It is required to
provide at least 5 standard sample, the concentration(activity) of first standard sample is zero, the
corresponding R equals to R0, other parameters can be determined with iteration method.
(6) Exponential-5P
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Calibration formula R=R0+Ke[alnC+b(lnC)2+c(lnC)3], with 5 parameters, which are R0,K,a,b and c. It is required to
provide at least 5 standard sample, the concentration(activity) of first standard sample is zero, the
(7) Polynomial-5P
Calibration formula LnC=a+b (R-R0)+c(R-R0)2+d(R-R0)3, with 5 parameters, which are R0, a, b, c and d. It is
required to provide at least 5 standard sample, the concentration(activity) of first standard sample is zero, the
(8) Spline
Calibration formula C-Ci=R0i+ai(C-Ci)+bi(C-Ci)2+ci(C-Ci)3-R, with 4i parameters, which are R0i,ai,bi and ci.
It is required to provide at least 2 standard sample, the parameters of each inter zone can be determined with
iteration method.
7.6 Concentration calculation

(1) When the item analytical method is dynamics method, calibration is not necessary, input the theoretical
calculation factor F directly, thereinto concentration equals to R and F product. For other type item,
must proceed calibration first, and obtain calibration parameter to calculate the concentration;
(2) If alibration type is linear calibration, Logit-4P or Polynomial-5P, the concentration can be calculated
using calibration parameter and reaction amplitude R directly;
(3) If calibration type is Logit-5P, Exponential-5P or Spline, calculate the concentration according to the
reaction amplitude R and calibration parameter with dichotomy to determine the positive real root.
7.7 Quality control (QC)
7.7.1 Quality control (QC) rule

The default quality control (QC) rule for SL-200 series is Westguard rule, the user can choose one or several
rules for QC status determination for different item according to actual requirement.
Westguard rule includes 10 subrule, the determination purpose of each subrule is as below:
Conventional Definition Quality control(QC)

letter Determination
12S 1 point fall out of the mean value +2SD or -2SD. Warning
13S 1 point fall out of the mean value +3SD or -3SD. Out of control(random error 、
system error)
22S 2 continuous points fall out of +2SD or -2SD. Out of control(system error)
R4S 2 continuous points exceed 4SD Out of control(random error)
41S 4 continuous points fall out of mean value +1SD or Out of control(system error)
-1SD.
10X 10 continuous points fall in the same side of the mean Out of control(system error)
value
The determination process for above subrule of SL 120 is as below:
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Quality control data
No
Under control
Yes
No No No No No
Yes Yes Yes Yes Yes
Out of control
7.7.2 Quality Control Type

The SL 120 consist of three types of quality control, namely real-time quality control, quality control in a day
and quality control among days. They can judge the quality control status respectively according to established
quality control rules.
Real-time quality control: to judge the quality control status of continuous 10 quality control data in one day;
Quality control in a day: to judge the quality control status of all the quality control data in one day;
Quality control among days: to judge the quality control status of all the quality control data among different
days;
7.7.3 Quality Control Chart

The SL 120 consist of three typs of quality control chart, namely L-J, cumulative sum quality control chart and
Twin Plot.
(1) Quality Control Chart L-J
With the measured value of quality control data as the ordinate, draw a horizontal line from the target value of
quality control. Then draw 6 lines parallelled with the mean line at upper +1SD (standard deviation, or SD for
short), +2SD, +3SD and at lower -1SD, -2SD, -3SD and mark ±1SD, 2SD and ±3 SD on the lines. Each value
of the quality control measured should be marked on the quality control chart with points, with the adjacent
points linked by fine lines.
(2) Cumulative Sum Quality Control Chart
Compute the cumulative sum of the quality control solution. With the cumulative sum as the ordinate and
times measured as the abscissa, Draw a horizontal line from 0 and then draw 2 lines paralled with the
horizontal line at control limit h (computed automatically according to the control rules selected by users in
quality control settings) of the cumulative sum in the above and below. Each cumulative sum should be
marked on the chart with points, with the adjacent points linked by fine lines, thus the cumulative sum quality
control chart is established. Any point above both of the upper and lower horizontal lines will be judged as out
of control.
(3) Twin Plot Quality Control Chart
When two levels of quality control solution are measured by one item, Twin Plot quality control chart will be
displayed. Based on the target value and standard deviation SD (input by users in the quality control settings)
of each quality control solution, with the measured value of one quality control solution (generally with low
concentration) as the abscissa and that of the other quality control solution (generally with high concentration)
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as the ordinate, the mean value as the centerline, and mark the lines ±1SD, 2SD and ±3 SD, with the value of
the two quality control solutions measured at the same time formed one point that will be marked on the
coordinate shown as Fig. 10-6:
3SD
2SD
1SD
-1SD
-2SD
-3SD
-3SD -2SD -1SD 1SD 2SD 3SD
Fig.10-6 Twin Plot Quality Control Chart

The graph can reflect sensitively the systematic and random errors, with the data falling in the blue circle (±
2SD) indicating that the system is under control, falling in the first or third quadrant between the red and blue
circle indicating that the system is error, falling in the second or fourth quadrant between the red and blue
circle indicating that the system is error randomly, falling outside the red circle indicating that the system is
also error randomly.
7.8 Other Relevant Computation
7.8.1 Corresponding Calculation of Calibration Curve

(1) Calibration Sensitivity
It means that if the D-value of response amplitude of the highest concentration calibration solution and the
lowest concentration calibration solution during calibration is less than the set value, it will be judged as fail.
(2) Response Amplitude of Blank Solution
It means that if the response amplitude value of the calibration solution with zero concentration is greater than
the set value, it will be judged as fail.
(3) Calibration Repeatability
It means that if the difference of the maximum and minimum value of the response amplitude measured in
three times for each calibration solution is greater than the set value, it will be judged as fail.
(4) Standard Deviation of Calibration Curve
Only applicable to multiple linear and nonlinear calibration curve. It means that the quadratic sum of the
'
difference between the reponse amplitude (R) of the calibration solution and the response amplitude ( Ri )
computed based on calibratiuon curve is divided by the degree of freedom and then extracted the square root.
The specific computing method is shown as follows:
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 Multiple Linear Calibrations

N n
 Rij  Ri 
i 1 j 1
' 2
SD =
Nn  2
Where, Rij, the response amplitude (effective measurement) measured for calibration solution i in one time;
Ri ' , the degree of response of calibration solution i computed based on the calibration curve; N, the number
of calibration siolution; n, times of effective repeated measurement.
 Logit-4P
N n
 Rij  Ri 
i 1 j 1
' 2
SD=
Nn  4
Where, Rij, the degree of response (effective measurement) measured for calibration solution i in one time;
 Logit-5P
N n
 Rij  Ri 
i 1 j 1
' 2
SD=
Nn  5
Ri ' , the degree of response of calibration solution i comupted based on the calibration curve; N, the number
 Exponential-5P and polynomial-5P
N n
 Rij  Ri 
i 1 j 1
' 2
SD=
Nn  5
 Spline
N n
 Rij  Ri 
i 1 j 1
' 2
SD=
Nn  4
(5) Factor Related to Calibration Curve
Only applicable to multiple linear and nonlinear calibration curves, the computational formula is shown as
follows:
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 Cij  C  Rij  R 
N n
2 2
i 1 j 1
R2 
  Cij  C   Rij  R 
N n N
2 2
i 1 j 1 i 1
Where, C, the concentration of the calibration solution; R, the response amplitude; N, the number of
calibration solution; n, times of effective repeated measurement.
Note:
SL 120 will measure each calibration solution for three times regularly and repeatedly.
The result with maximum deviation will be deleted, and the other two results will be
taken an average value, hence n equals 2 fixedly.
7.8.2 Substrate Exhaustion Judgement

This judgement is only applicable to kinetic method and two-point method. Because some high concentration
samples (activity) makes the substrate exhaust quickly, which makes the reaction speed can not reach the
expected speed (level 0 or level 1 reaction), in order to correctly reflect the result the substrate exhaustion
should be judged. The specific judgement method is as follows:
(1) Ascending Reaction
If the absorbance value of any point or multiple points in the begaining and ending period is greater than the
set value, it will be judged as substrate exhaustion.
(2) Descending Reaction
If the absorbance value of any point or multiple points in the begaining and ending period is less than the set
value, it will be judged as substrate exhaustion.
7.8.3 Linearity Check

The linearity check, only applicable to kinetic method, is used to judge whether the Linearity of reaction curve
meets the set value in the beginning and ending period of reaction, based on the data of all metering points.
The detailed computing method is as follows:
(1) The number of metering points in the beginning and ending period is more than 9
Linear limit = (the absorbance change rate of the first 6 points – that of the latter 6 points) / the absorbance
change rate of all points
(2) 4≤the metering points in the begining and ending period≤8
Linear limit = (the absorbance change rate of the first 3 points – that of the latter 3 points) / the absorbance
change rate of all points
(3) The linearity will not be computed for the following situations:
 The number of metering points ≤3
 The absorbance change rate is less than 0.006 / minute, or the D-value of the absorbance change rate is
less than 0.006/minute.
 The reagent blank test, sample blank test and calibration solution test of zero-dose
7.8.4 Prozone Check

In the antigen-antibody reaction, the insoluble antigen-antibody complex generated is closely related to the
ratio of antigen-antibody. In the appropriate rate, the insoluble antigen-antibody complex amount generated is
the most, while the light filtered is the least, equivalently the absorbance is the most. More than or less than the
ratio, the insoluble antigenic antibody complex amount generated will be reduced, while the light filtered will
be increased and the absorbance will be lessened, shown as below figure: If the prozone check is not
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conducted, the insoluble antigen-antibody complex amount generated by two samples with very large different
concentration can be equal, which will cause the same test result.
Fig. 10-7 Schematic diagram of the relationship between insoluble antigen-antibody complex and the ratio of
antigen-antibody
In SL 120, the prozone check can be conducted according to the following methods:
(1) Double Reagent End Point Method
Shown as the following figuer, L is for reaction starting point, M is for reaction ending point, N and P are for
prozone check point. L, M, N and P should meet the following relationship:
13≤L＜26≤N＜P＜M≤77
Fig.10-8 Schemtaic diagram of the prozone check for double reagent end point method
The value of the prozone check (PC) is equal to:
AM  A P
PC  M  P  100%
AP  AN
PN
If PC is less than the prozone check limit, it can be judged that the prozone phenomenon is existed;
(2) Single Reagent End Point Method
Shown as the following figuer, L is for reaction starting point, M is for reaction ending point, N and P are for
prozone check point. L, M, N and P should meet the following relationship:
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1≤L＜13≤N＜P＜M≤77
Fig.10-8 Schemtaic diagram of the prozone check for single reagent end point method
The value of the prozone check (PC) is equal to:
AM  A P
PC  M  P  100%
AP  AN
PN
If PC is less than the prozone check limit, it can be judged that the prozone phenomenon is existed;
7.8.5 Reaction Equilibrium Judgment

This judgement is only applicable to the end point method and is ued to judge whether the reaction equilibrium
in the ending time has been reached based on the data of all metering points. The detailed computing method is
as follows:
(1) Compute the D-value of the absorbance between the end point and the following continuous 3 metering
pints;
(2) If all D-values are less than 0.01, it can be judged that the equilibrium has been reached, otherwise the
equilibrium has not been reached;
(3) If the reaction endpoint is greater than 75, the reaction equilibrium judgement will not be conducted.
7.8.6 Lamp Bulb State Judgement

(1) Some wavelength luminous intensity is equal to:
Average value of the water blank AD values of all cups of this wavelength - dark current of this
wavelength
Among: the average value means the average value of residual values after removing plus or minus 2 times the
standard deviation
(2) When the luminous intensity of any wavelength is less than 60% of factory value, it indicates that the
lamp bulb needs to be changed;
7.8.7 Reaction Cup Clean State Check

(1) The transmission value of some wavelength is equal to:
The water blank AD value of this wavelength - dark current of this wavelength
(2) Any reaction cup which meets any of the following situations will be judged as dirty cup.
- 100 -
 The transmittance value of any wavelength is less than 40% of factory value;
 The transmittance value of any wavelength falls outside of plus or minus 3 times standard deviation of
transmittance average value of all cups.
Chapter 8 Abnormal Model and Cause Analysis of Common

Results
8.1 Reactive Curve Fluctuates and Beats
1. Nearly all reaction curves beat
 Poor power ground: the main power source ground and photoelectric box ground are poor
 Stirring rod without stiring: the stirring motor is bad; the line contact of stirring motor is poor; and
the stirring rod bears against the bottom of reaction cup.
 Crooked light path: the lamp bulb, reaction cup and temperature control loop are installed askewly;
the postion of lens is moved
 Abnormally reagent adding: reagent needle stops up; the reagent injector, reagent needle joint and
quick joint are off
 The sunlight beat down on the photoelectric reaction system
 External interference factor: for instance the engine, electric drill etc.
2. Only the individual wavelength reaction curve beats
 Poor power ground: the main power source ground and photoelectric box ground are poor
 The lens mildews
2. Only the individual item reaction curve beats
 Abnormal reagent: the reagent becomes muddy, discoloring and is misplaced
 After of stirring, the reagent produces many bubbles which block the light path
8.2 Abnormal Reaction Curve

1． Nearly all reaction curve shapes are correct and the reaction curve is smooth, but there is no reaponse
basically
 No sample added: the adding sample needle stops up; the sample injector, adding sample needle joint
and quick joint are off
2． The indivisual item reaction curve shape is correct and the reaction curve is smooth, but there is no
reaponse basically
 No second reagent added: the second reagent is not added in bottle or is misplaced
3． The indivisual item reaction curve shape is changed, but the reaction curve is smooth
 Abnormally reagent adding: the pin for fixing the reagent disk is off; or the reagent is misplaced
4． The indivisual item reaction curve shape is changed, ascending or descending sharply, and the reaction
curve is either smooth or fluctuant slightly.
 Abnormal reagent: the reagent performance is poor or expired, most easily occurred on items such as
ALP, GGT, AMY etc.
5． The indivisual item reaction curve shape is changed, ascending or descending sharply, but the reaction
curve is smooth.
 Cross contamination among reagent: it most easily occurs on items such as TG, TC, Glu etc.
 Abnormal sample: sample contains some medicines or interference element, which most easily
- 101 -
occurs on items such as TB, DB etc.
8.3 Normal Reaction Curve, Poor Repeatability of the Results

1． Nearly the repeatability of all items is poor
 Abnormally sample or reagent adding: adding sample needle stops up; there are bubbles in sample
injector; adding sample needle joint sealing is not tight; adding sample needle inner wall valve
closure is not tight
 Abnormal stirring: the stirring motor is bad; and the line contact of stirring motor is poor; and the
stirring rod bears against the bottom of reaction cup.
 Inadequate cleaning for the internal and external walls of reagent / sample and potential cross
contamination: the liquid pump pressure drops, and the 2nd stage filter plugs.
 Unstable reaction disk temperature: the reaction disk temperature fluctuation is relatively large.
2． The repeatability of individual item is poor
 Reagent is misplaced
 Abnormal reagent: the reagent performance is poor or expired, most easily occurred on items such as
ALP, GGT, AMY etc.
 Abnormal item parameter settings: few samples; short reaction time; error reaction method setttings
 Abnormal calibration: error calibration solution concentration settings, expired calibration solution
8.4 Normal Reaction Curve, Results Is Zero or Even Lower

1． Nearlly all items results are zero or even lower
 Abnormally sample adding: adding sample needle stops up; there are bubbles in sample injector;
adding sample needle joint sealing is not tight; adding sample needle inner wall valve closure is
not tight
 Abnormal stirring: the stirring motor is bad; and the line contact of stirring motor is poor; and the
stirring rod bears against the bottom of reaction cup.
2． The individual item result is zero or even lower
 The regent blank test is abnormal
 Reagent misplaced: especially the second reagent is misplaced.
 Reagent expired or discolored: it most easily occurs on items such as ALP, GGT, AMY etc.
 Less reagent
3． The test result of individual item is negative.
 Reagent is expired or discolored.
 Abnormally sample adding: adding sample needle stops up; there are bubbles in sample injector;
adding sample needle joint sealing is not tight; adding sample needle inner wall valve closure is
not tight
 Second reagent is misplaced or is less
8.5 Normal Reaction Curve, Results Falls outside Quality Control

1． Nearly the quality control results of all items fall outside the scope of quality control
 Quality control solution is misplaced.
 Quality control solution is expired.
- 102 -
2． The quality control results of individual item fall outside the scope of quality control
 Reagent is expired.
 The calibration solution and quality control solution are not matched
8.6 Normal Reaction Curve, Results are Higher Or Lower Generally

1. All results are higher or lower generally
 The cup blank overflows; the AD value of cup blank is 32768 or 0.
 Abnormal stirring of stirring rod: the stirring motor is bad; and the line contact of stirring motor is
poor;
 Abnormal sample adding: the adding sample needle stops up; the electromagnetic valve can not be
closed tightly; the level surface detection is abnormal.
2. The result of individual item is either higher or lower generally
 Abnormal reagent: the reagent performance is poor; and the reagent is expired, mixed, discolored
etc.
 Abnormal calibration: the calibration solution concentration is input error; the calibration solution is
misplaced and expired.
- 103 -
Chapter 9 Common Operations and Regular Maintenance

9.1 Preparation of Tools
(1) M3 and M4 inner hexagon spanner;
(2) Cross screwdrivers (large, medium and small);
(3) Stainless steel wire (Φ0.3mm);
(4) Plastic injector (about 10ml, without needle);
(5) Clean gauze;
(6) Clean cotton swab;
(7) Brush (used for cleaning the barrel);
(8) Non-ionic surfactant cleaning agent;
(9) Absolute ethyl alcohol;
(10) 84 disinfectant fluid;
(11) Latex medical gloves
9.2 Common Operations
9.2.1 Installation and Replacement of Cuvette

Cuvette for SL-200, is semipermanent and is needed to be every 2 months.
Note:
While placing new set of cuvettes, do not touch the optical surface of the
reactionreaction vessel by hand.
Do use the cuvettes specially provided by Biosino only to ensure the accuracy of
the test results.
9.2.2 Replacement of Reagent Bottles

The instrument holds a total of sixty 60ml&20ml reagent bottles. Do check that the bottle tops are aligned
while placing them.
9.3 Daily Maintenance
9.3.1 Wiping Analyzer Table

There may be some reagent, reactant liquor and sera left on the analyzer table, clean the table everyday after
the power-off as the following steps:
1 Clean the analyzer table with wet towel absorbing cleaning fluid until all the stains are erased;
2 Clean the analyzer table with the wet towel absorbing disinfectant fluid;
3 Fifteen minutes later, remove the disinfectant fluid on the table with clean wet towel, which is
wring out.
- 104 -
Note: Corrosion
The cleaning fluid is chemically corrosive. During the operation, do wear protective
gloves.
Note: Biological Pollution

The table should be deemed as the source of infection. During the operation, do wear
protective gloves.
9.3.2 Cleaning the Sampling Probe/Stirring Rod

The needle outer wall and point may have some sera, reagent and water left, if not properly cleaned. Check
each day after power-off, and clean the needle if it is not clean. To clean it, soak the clean cotton swab into the
absolute ethyl alcohol, and then clean the sampling probe point with the cotton swab, until there is no visible
attachment.
Note: Combustion
The ethanol is combustible. During the cleaning operation, make sure the analyzer is
power off. Use no more than 10ml ethanol in areas adjacent to the analyzer.

All parts should be deemed as the source of infection. During the operation, do wear
protective gloves.
9.3.3 Cleaning the Needle Tube/Suction Nozzle of the Cuvette Wiper

Mechanism
If the needle tube of the wiper mechanism is left uncleaned, there may be some reactant liquor and water
attached to it. Check before power-off each day. In case of situations mentioned above, please clean in the
following steps (applicable only to those with the self-cleaning system):
1 Clean the discharge needle tube and point with cotton swab absorbing absolute ethyl
alcohol, until there is no visible attachment;
2 Clean the take-in needle tube and point with cotton swab absorbing absolute ethyl
alcohol, until there is no visible attachment;
Figure: Cleaning the Suction Nozzle of Wiper Mechanism
1 Clean the four sides, top and bottom of the nozzle with clean cotton swab absorbing
purified water, until there is no visible attachment;
2 Clean the four sides, top and bottom of the nozzle with clean cotton swab absorbing
absolute ethyl alcohol, until there is no visible attachment;
- 105 -
Note:
Note that during the cleaning, there may be some cotton fiber from the cotton swab left
between the drainage and take-in needle tubes. In this case, clean the fiber in time.
Note: Combustion
The ethanol is combustible. During the operation, make sure the analyzer is power off.
Use no more than 10ml ethanol in areas adjacent to the analyzer.

All parts should be deemed as the source of infection. During the operation, do wear
protective gloves.
9.4 Weekly Maintenance
9.4.1 Cleaning the Case

Clean the case as the following order:
1 Cut off the power supply of the system. Make sure that the power cord is disconnected from the
power grid
2 Open the upper shield, and clean the workbench with clean water
3 After cleaning the workbench, clean the interior part of the upper shield.
4 Then open the front door at the lower part off the instrument, and clean the inside and outside
walls of the door
5 Finally, clean both sides of the instrument, and the back side of the case
Cautions:
1. Do not plug in the power cord and start the instrument immediately after cleaning the case. Start the
instrument when you make sure that the surface of the instrument case is free of water.
2. Do not put the reagent bottle on the workbench when the instrument is working, lest the reagent bottle
should fall over and the reagent flow into the instrument. This may shorten the use life of the
instrument.

All waste liquids should be deemed as the source of infection. During the operation, do
wear protective gloves.
9.4.2 Automatic Flushing the Stirring Rod, Sampling Probe and Pipeline
Prepare the following stuff: the reagent bottle containing 35ml cleaning fluid, and sample cup. Clean as the
following steps:
1 Put the special cleaning fluid in the sample cup
2 Put the reagent bottle holding 35ml cleaning fluid in the designated position on the reagent tray
- 106 -
3 Power on and the system initiation starts. When the system is in standby mode, run the
automatic cleaning program.
4 Run the cleaning program as needed. The entire process is about 5 minutes
Cautions:
1. Keep the hand and clothes clear of the cleaning fluid. In case of touching the cleaning fluid, clean with
water immediately. If, however, the fluid is in your eyes or mouth, clean with water and see the doctor
immediately.
2. Keep the instrument clear of the cleaning fluid. Or else, clean the fluid with clean cloth or paper.
Finally clean it with dry cloth 2-3 times.
3. Do not mix the cleaning fluid with other chemicals. In case of such mixture, neutralize properly and
discard the mixture.
9.4.3 Cleaning the Reagent Sample Tray

1 Power off;
2 Open the upper cover and sample tray lid. As shown in the Figure below, two black fasteners are
used to secure the sample tray
3 Lift the two fasteners with proper force. As shown in the Fig. below, you can see the bottom of the
tray
4 Take out the ragent bottle on the tray. Clean the reagent tray and its bottom with gauze absorbing
the cleaning fluid, until there is no visible stain, then clean with dry gauze.
5 Mount the sample tray and cover the lid and upper cover.

All stains should be deemed as the source of infection. During the operation, do wear
protective gloves.
9.5 Monthly Maintenance
9.5.1 Cleaning the Wash Tank

1 Power off;
2 Remove the sampling probe from its cleaning position;
- 107 -
3 Clean the inside and outside walls of the wash tank of sampling probe with cotton swab
absorbing the cleaning fluid, until there is no visible stain. Then clean with dry gauze;
4 Remove the stirring rod from its cleaning position;
5 Clean the inside and outside walls of the wash tank of the stirring rod with cotton swab
absorbing the cleaning fluid, until there is no visible stain. Then clean with dry gauze;

protective gloves.
9.5.2 Cleaning the Reaction Tank

1 Open the upper cover and reaction plate cap. Screw off the three screws fixing the reaction tray
with a screwdriver.
2 Lift the reaction plate with proper force. Clean all the parts of the inside wall (indicated in red) of
the reaction tank with gauze absorbing the cleaning fluid, until there is no visible stain. Then clean
with dry gauze.
3 Mount the reaction tray, and fasten the screws;
4 Cover the reaction plate with the lid.

protective gloves.
- 108 -
9.6 Unscheduled Maintenance
9.6.1 Unclogging the Sampling Probe

When the probe is clogged, it needs to be unclogged immediately.
1 Power off;
2 Turn the sampling probe to an appropriate position, and open the top cover of the probe rocking
arm
3 Unscrew the two screws fastening the probe.
4 Unplug the wire to liquid level checkout console and the joint with the tube. Loosen the holddown
spring.
5 Lightly lift the sampling probe
6 Unclog the sampling probe with Φ0.3mm stainless steel wire from the probe point repeatedly.
7 Connect the one-shot injector with an appropriate hose on one end. The other end of the hose is
connected to the rapid acting coupling. Inject water into the probe. That the water shooting out in a
line from the probe indicates the probe has been unclogged;
8 Mount the probe and cover the rocking arm in the reverse order;

The sampling probe should be deemed as the source of infection. During the operation,
do wear protective gloves.
9.6.2 Replacement of the Sampling Probe

In case of an uncloggable, bent or broken probe, the probe needs to be replaced immediately.
Before replacement, make sure that the sampling probe is above the wash tank, as liquid may
1
drop off during the replacement;
2 Check that the system is on, and in the warm-up or stand-by mode;
3 Remove the old sampling probe according to the steps to unclog the sampling probe;
Insert the new sampling probe from the point, connect the probe with the joint and with liquid
4 level monitoring console. Ensure that there is no leaks at the joint. Tighten the screws fastening
the sampling probe;
Start up the system, and reset twice or three times, until the tube is free of air. When the tube is
5
free of air, water will jet out from the probe point. Check that the water jets out properly;
Mount the sampling probe cover. Ensure that the cover is securely in place as indicated by a clip
6
sound
7 Close the upper cover
- 109 -

The sampling probe should be deemed as the source of infection. During the operation,
do wear protective gloves.
9.6.3 Replacement of the Stirring Rod

In case of a bent or broken rod or a rod that is frequently attached with liquid, the rod needs to be replaced
immediately.
1 Make sure that the system is power off
2 Loosen the two screws fastening the stirring rod, and take out the stirring rod to be replaced.
3 Insert the new stirring rod into the motor shaft in the bottom-top direction. Tighten the screws
fastening the stirring rod.
4 Rotate the stirring rod lightly, and make sure that there is no marked movement.

The stirring rod should be deemed as the source of infection. During the operation, do
wear protective gloves.
9.6.4 Replacement of the Light Bulb

Where the light bulb in used for over six months or the analyzer indicates the replacement of the light bulb, the
light bulb should be replaced immediately in the following steps:
1. Open the bench plate, and you can see the red holder connected to the bulb;
2. The two screws on the red holder are used to fasten the power cord. Loosen the screws with a screwdriver,
and unplug the power cord from the holder;
3. Remove the rotating disc or coding disc of the cuvette tray with a cross screwdriver
4. Rotate the cuvette tray to the appropriate position, and you can see the light. Loosen the two screws
fastening the holder, and remove the light bulb with the holder.
5. Fasten the light bulb and holder .
Note: Hight Temperature and Burns

Before replacing the light bulb, turn off the power and wait for at least 30 minutes
until it is cooled down. Then remove the light bulb.
- 110 -
Note: Strong Light

Before replacing the light bulb, make sure that the instrument is power off. Or
else, the light beam from the bulb may hurt your eyes.
Note: Fallen Screws

While loosening or tightening the screws, be careful to prevent the screws from
falling off.
Note: Photoelectric Correction Inspection

After replacing the light bulb, you must carry out the photoelectric correction
inspection.
9.6.5 Replacement of the Injector Pump

1. Open the left-hand side panel of the instrument, and you will see the sample injector on the right and
reagent injector on the left;
2. Loosen the connector of teflon tube and injector pump; (two for each injector pump);
3. Remove the connector to the motor and transducer wires of the injector pump;
4. Loosen the mounting screws of the injector pump with a screwdriver;
5. Remove the screws and take off the injector pump;
6. Tighten the mounting screws of the replacing injector pump;
7. Connect the teflon tube and injector pump with the connector (replace the connector in time in case of
damage);
8. Mount the connector to the motor and transducer wires of the injector pump;
10. Close the left-hand side panel
9.6.6 Washing the Pipeline System
Check the purified water barrel as the following order:
1 Open the barrel lid
2 Pour in the cleaning fluid of effective concentration.
3 Power the system up.
4 Make the instrument perform initialization, reset and self-check cleaning repeatedly;
5 Open the clean water barrel, and clean the bottle and pipeline with remaining cleaning agent.
Then rinse with clean water.
6 Pour in the clean water, and repeat Step 4 and 5
7 Close the front door of the instrument
- 111 -
9.6.7 Wiping the Drive Rod

1 Power off;
2 Move the stirring rod, so that the drive rod is turned to an angle suitable for wiping;
3 Wipe the drive rod with clean gauze until there is no visible dust and stain. Then apply the
lubricating oil, and pull the drive rod up and down, so that the lubricating oil is evenly aplied to
the rod;
- 112 -

BA-A-S120Mini Software Operating Instructions

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BA-A-S120Mini Software Operating Instructions

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BA-A-S120Mini

Chapter 1 Introduction..... ............................................................................................................4

1.1 Product overview ............................................................................................................................................ 4

1.1.1 Product introduction...................................................................................................................4

1.1.2 Basic performance.......................................................................................................................5

1.1.3 Main structure..............................................................................................................................5

1.1.4 Scope of application.....................................................................................................................5

1.2 Symbol, chart displays, label and picture .................................................................................................... 6

1.2.2 Chart display and label......... .....................................................................................................7

1.3 Warranty and service ..................................................................................................................................... 7

1.4 Training ........................................................................................................................................................... 8

1.5 Explanation of terms .................................................................................................................... 9

1.6 Explanation of terms .................................................................................................................... 9

Chapter 2 Basic operation Procedure ..................................................................................................... 15

Chapter 3 Basic Test Procedure ................................................................................................ 18

3.1 Reagent Black Test Procedure .................................................................................................................... 18

3.2 CAL Test Procedure .....................................................................................................................................18

3.3 General Test Procedure ............................................................................................................................... 19

3.4 QC Test Procedure ....................................................................................................................................... 20

Chapter 4 Operational procedure for new items ..................................................................... 23

Chapter 5 Routine operational procedure ................................................................................24

5.1 Check before Power-on ................................................................................................................................ 24

5.2 Power-on ........................................................................................................................................................ 24

5.3 Run the Program .......................................................................................................................................... 24

5.4 Test request ................................................................................................................................................... 24

5.5 Test preparation ............................................................................................................................................24

5.6 Start the test .................................................................................................................................................. 25

5.7 Test results confirmation ............................................................................................................................. 25

5.8 Exit the program ...........................................................................................................................................25

5.9 Power-off ........................................................................................................................................................26

5.10 Check after power-off ................................................................................................................................ 26

Chapter 6 Interface operation ....................................................................................................27

6.1 Login interface .............................................................................................................................................. 27

6.2 General setting .............................................................................................................................................. 33

6.3 Test setting-up ...............................................................................................................................................47

6.4 Statistics/Query ............................................................................................................................................. 59

6.5 Maintenance .................................................................................................................................................. 65

6.6 Shortcut buttons ............................................................................................................................................76

6.7 Instrument Running Status Diagram ......................................................................................................... 91

Chapter 7 Computing method ................................................................................................... 92

7.1 Test process ................................................................................................................................................... 92

7.2 Metering point ...............................................................................................................................................92

7.3 Analytical method .........................................................................................................................................92

7.4 Absorbance and reaction amplitude ...........................................................................................................94

7.5 Calibration .....................................................................................................................................................95

7.6 Concentration calculation ............................................................................................................................96

7.8 Other Relevant Computation ...................................................................................................................... 98

8.1 Reactive Curve Fluctuates and Beats ....................................................................................................... 103

8.2 Abnormal Reaction Curve .........................................................................................................................103

8.3 Normal Reaction Curve, Poor Repeatability of the Results ...................................................................104

8.5 Normal Reaction Curve, Results Falls Outside QC ................................................................................104

Chapter 9 Common Operation and Regular Maintenance ...................................................106

9.1 Preparation of tools .................................................................................................................................... 106

9.2 Common Operations .................................................................................................................................. 106

9.3 Daily Maintenance ...................................................................................................................................... 106

9.4 Weekly Maintenance .................................................................................................................................. 108

9.5 Monthly Maintenance ................................................................................................................................ 109

9.6 Unscheduled Maintenance ......................................................................................................................... 110

Management classification: It belongs to biochemical analysis system in clinical examination analytical

Semi-wave width No more than 12nm

Sdisk light Absorbance no less than 2.5

Temperature accuracy Temperature accuracy should be ±0.2 ℃ , temperature fluctuation should be

Adding sample error ≤±4%, variable coefficient ≤2% when sample is 2

Input power 150W