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    The document provides instructions for a practical lab on testing carbohydrates, lipids, and proteins in foods. Students are asked to test samples for starch, sugars, proteins, and lipids using various reagents. They must record their observations of any color changes in a results table. The discussion questions prompt students to explain the principles behind each test, such as how iodine detects starch and copper sulfate functions in Benedict's solution for sugars. Students must write a lab report discussing their results and answering the questions.

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    Practical 2 Food tests

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    The document provides instructions for a practical lab on testing carbohydrates, lipids, and proteins in foods. Students are asked to test samples for starch, sugars, proteins, and lipids using various reagents. They must record their observations of any color changes in a results table. The discussion questions prompt students to explain the principles behind each test, such as how iodine detects starch and copper sulfate functions in Benedict's solution for sugars. Students must write a lab report discussing their results and answering the questions.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    2 views2 pages

    Practical 2 Food Tests

    Uploaded by

    waulamoro
    The document provides instructions for a practical lab on testing carbohydrates, lipids, and proteins in foods. Students are asked to test samples for starch, sugars, proteins, and lipids using various reagents. They must record their observations of any color changes in a results table. The discussion questions prompt students to explain the principles behind each test, such as how iodine detects starch and copper sulfate functions in Benedict's solution for sugars. Students must write a lab report discussing their results and answering the questions.

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    © All Rights Reserved
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    of 2

    The University of Goroka

    School of Science and Technology


    Division of Biological Sciences

    Semester 1, 2024 BI 131: Practical 2

    Due date: Following week on your practical days. Place the report in the BI 131 PH in Biology Division.

    Units: Carbohydrates, Lipids & Proteins


    _________________________________________________________________________________

    Nutrition Food Tests


    --------------------------------------------------------------------------------------------------------------------------------------

    Objective: To test for starch, sugar, protein, and lipids

    Materials

    - Solution A (water), solution B, Solution C, solution D, solution E, Iodine solution


    - Test tubes, Test tube holder, Test tube rack, 5% sodium hydroxide solution, 1% copper sulphate solution,
    Heater, Benedict’s solution, Ethanol, Dropper, Filter paper, Measuring cylinder, small beakers, Glass rods.

    Procedure and Results

    (Instruction: Write yes, if there is a colour change and name the colour that shows in the experiment.)

    Discussion Questions: Use these questions to help you with your discussion.

    Question 1.1 Draw and describe the starch structure.


    1.2 Which of its structural feature is important to the color change in iodine test?
    1.3 What is the composition of iodine?
    1.4 How does color change takes place in the iodine test?

    Question 2.1 State the principle of Benedict’s test on sugar.


    2.2 What is the function of copper sulphate in Benedict’s Solution?

    Question 3.1 What is the Biuret?


    Prepared by: Sheila Take Kotop Semester 1, 2024 1
    3.2 State the composition of Biuret Reagent.
    3.3 Will amino acids test positive or negative? Why?

    Question 4.1 What is the emulsion test?


    4.2 How does an emulsifier works?
    4.3 Differentiate between hydrophobic and hydrophilic emulsion?
    4.4 Describe physical states of fats and oils.

    Note: Write a lab report following the lab report format that was given during lecture in week 2. Use the questions to
    discuss the results in the table. Practical report will be assessed.

    Prepared by: Sheila Take Kotop Semester 1, 2024 2

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