Far Eastern University

Manila

Department of Medical Technology

MTY 1227 HISTOPATHOLOGIC TECHNIQUES

WORKSHEET No. 1

FIXATION
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1. In your own words, provide a definition of the step-in tissue processing, fixation.

2. Classify fixatives that you have learned, based on:

A Nature
B. Composition
C. Action
 3. Differentiate the different fixatives demonstrated in the laboratory class, completing
the following table:

NAME OF CLASSIFICATION ACTIVE PURPOSE/ FIXATION PRECAUTIONS

FIXATIVE COMPONENTS USE TIME

4. Explain the process of creating 5L of 10% Formaldehyde.

 Far Eastern University
Manila

Department of Medical Technology

MTY 1227 HISTOPATHOLOGIC TECHNIQUES

WORKSHEET No. 2

DEHYDRATION and DECALCIFICATION

_________________________________________________________________________________________________________

1. Define what dehydration is all about?

2. Explain by means of illustration and exposition, the principle of dehydration.

3. Differentiate the advantages and the disadvantage of the following as
dehydrating agent:
A. Ethyl Alcohol
B. Acetone
C. Dioxane
D. Tetrahydrofuran
E. Butyl Alcohol

Dehydrating Agent Advantages Disadvantages

4. What is the purpose of putting the specimen back to lower concentration of

alcohol, then proceeding to usual process, if the specimen is placed
inadvertently to the highest or pure concentration?
5. DIFFERENTIATE THE DIFFERENT DECALCIFICATION AGENTS.

6. WHAT ARE THE MECHANISMS AVAILABLE TO ASSESS COMPLETENESS OF

DECALCIFICATION?
 Far Eastern University
Manila

Department of Medical Technology

MTY 1227 HISTOPATHOLOGIC TECHNIQUES

WORKSHEET No. 3

Clearing

_________________________________________________________________________________________________________

1. Define Clearing.

2. What are the characteristics of a good clearing agent; explain BRIEFLY.

 3. Differentiate the different clearing agent/s demonstrated in the laboratory class,
completing the following table:

NAME OF PURPOSE/ USE FIXATION PRECAUTIONS

CLEARING TIME
AGENT

4. Why is clearing an indispensable stage in the tissue processing?

 Far Eastern University
Manila

Department of Medical Technology

MTY 1227 HISTOPATHOLOGIC TECHNIQUES

WORKSHEET No. 4

INFILTRATION AND EMBEDDING

_________________________________________________________________________________________________________

1. What is/ are the purpose of infiltration and embedding? Discuss the most
common medium used.

2. What are the materials that are commonly used in this stage in tissue
processing?

3. Explain orientation.
4. Differentiate the different types of embedding process.

Type of embedding Medium primarily used Use/ utilization

 Far Eastern University
Manila

Department of Medical Technology

MTY 1227 HISTOPATHOLOGIC TECHNIQUES

WORKSHEET No. 5

Microtomy/ Cutting

_________________________________________________________________________________________________________

1. Discuss the main principle of microtomy.

2. Differentiate the different types of microtomes:

TYPE OF USE ADVANTAGES DISADVANTAGES

MICROTOME
ROTARY

ROCKING

SLIDING

UTRATHIN

FREEZING
3. DRAW THE MICROTOME AND LABEL PARTS.

4. What are the ways on how you can take good care of the microtome?

5. Differentiate Honing from stropping.