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Pharmacology
Pharmacology
Pharmacology
In this report the two distinct experiments will be performed. Experiment 1 is the simulation of
drug effects on the guinea pig ileum and for experiment 2 the separation of proteins in the human
leukemic cell homogenates using 1D SDS-PAGE gel is performed.
Experiment 1
Dose Response
120
100
80
60
40
20
0
-9 -8 -7 -6 -5 -4 -3 -2
Control
Atropine 2E -8 atropine 2E-7
atroine 2E-6
Figure 1. The dose response graph of three different concentrations of atropine and control.
Based on the Schild plot, the consequence of atropine in terms of form of antagonism was
projected, and pA2 values were obtained. The findings indicated that atropine provided a dose-
dependent increase in response to an increase in atropine concentration, but that the increase in
dose response was very minor. In the case of higher concentrations, the dose response measured
is 60 mM, although it stays similar to 63 nM. Atropine the concentration response curve shifted
toward right with a change in EC50 value. The value calculated for 100nM atropine is 7.9 which
is almost similar to the previous values. Schild plots indicated that antagonism formed by
atropine was regarded as competitive in nature as shown in figure 2. The pA2 values of atropine
were found significantly high.
Hypotheses
In this experiment we will test
two hypotheses:
1. ACh and histamine will
cause a concentration-
dependent contraction of the
guinea pig
ileum sections