SCHEME: RECOMBINANT DNA TECHNOLOGY TOOLS & TECHNIQUES

The scheme is an overview of work-integrated learning opportunities and gets students out into the real
world. This will give what a course entails.

RECOMBINANT DNA TECHNOLOGY

Course Title Course Type Theory
TOOLS & TECHNIQUES
B.Sc. Medical
Course Code Q1UG402T Class
Biotechnology
Activity Credits Credit Hours Total Number of Classes Assessment in
Lecture 4 4 per Semester Weightage

Instruction Tutorial 0 0

Self-study
Practical
Tutorial
Theory
delivery

SEE
CIE
Practical 0 0
Self-study 0 0
Total 0 0 100 50% 50%
Course
Course Lead Dr. Charu Gupta Dr. Charu Gupta
Coordinator
Theory Practical
Names
Course
Dr. Charu Gupta NA
Instructors

COURSE OVERVIEW
1) This course will help student understand various techniques used in Biotechnology.
2) The student will understand different instruments and their utilization in various biotechnological
techniques.

PREREQUISITE COURSE
PREREQUISITE COURSE YES
REQUIRED Bachelor’s degree in biology or any other relevant
field from a recognized university with a minimum
of 50% marks in aggregate.
If, yes please fill in the Details Prerequisite Prerequisite course name
course code
Q1UF104C Molecular Biology

COURSE OBJECTIVES
This course will explain various instruments and techniques used in medical Biotechnology, including
but not limited to: centrifugation, chromatography, microscopy, etc.

COURSE OUTCOMES (COs): After completion of this course work students able to

COURSEPACK | FORMAT
 402T.1 Understand the basic concepts in genetic engineering and the enzymes used in the process
402T.2 Polymerase Chain Reaction
402T.3 Tools of Genetic Engineering and Cloning Vectors
402T.4 Molecular Probes and Method of Gene Transfer
402T.5 Analysis and Expression of gene in host cells
402T.6 Gene Libraries, Isolation, Sequencing and Synthesis of genes

BLOOM’S LEVEL OF THE COURSE OUTCOMES:

CO No. Remember Understand Apply Analyze Evaluate Create
KL1 KL2 KL3 KL4 KL5 KL6
402T.1 √
402T.2 √ √
402T.3 √
402T.4 √
402T.5 √
402T.6 √

COURSE ARTICULATION MATRIX

PO # CO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2
402T.1 3 2 2 3 3 3 2 3
402T.2 3 2 2 3 3 3 2 3
402T.3 3 2 2 3 3 3 2 3
402T.4 3 2 2 3 3 3 2 3
402T.5 3 2 2 3 3 3 2 3
402T.6 3 2 2 3 3 3 2 3
Note: 1-Low, 2-Medium, 3-High

COURSE ASSESSMENT
CIE Total Marks Grand
Type of Course Weightage
Total
(B) IA1 MTE IA2 CIE SEE (CIE-SEE)
Marks
Theory 25 50 25 100 100 200 50-50
#Rubric for the Internal Assessments
Type of Assessment Tools QUIZ AAT$ /MOOC
Internal Assessments 10 15
$AAT is Literature survey, Seminar, Term Paper, Slip Test (or) MOOC Certificate relevant to the course

COURSEPACK | FORMAT
COURSE CONTENT (THEORY)
Unit 1 - Basic concepts and Tools of Genetic Engineering: Enzymes 15 Hours
Basic terminologies and concept, History, Scope and Future; Restriction enzymes, Modifying
enzymes, DNA ligase, Polymerase etc.
Unit-2: Polymerase Chain Reaction 15 Hours
Basic principles modification and applications of PCR Technique, Various types of PCR techniques
and mutagenesis
Unit-3 Tools of Genetic Engineering: Cloning Vectors 15 Hours
Cloning vehicles, Plasmids, Lambda- phage, Phagemids, Cosmids, Artificial chromosomes (BACs,
YACs), Shuttle vectors, virus-based vectors.
Unit -4 Molecular Probes and Method of Gene Transfer 15 Hours
DNA probes, RNA probes, Radioactive labeling, non- radioactive labeling, use of molecular
probes, DNA fingerprinting, Transformation, transduction, Particle gun, Electroporation, liposome
mediated, microinjection, Agrobacterium mediated gene transfer
Unit -5 Analysis and Expression of gene in host cells 15 Hours
Expression vectors, Restriction enzyme analysis, Southern blotting, Northern blotting, Western
blotting, In-situ hybridization. Colony and plaque hybridization, Factors affecting expression of
cloned genes, Reporter genes, Fusion proteins.
Unit-6 Gene Libraries, Isolation, Sequencing and Synthesis of genes 15 Hours
cDNA synthesis, Genomic DNA libraries, Amplification of gene libraries, Identifying the products
of cDNA clones. Different methods of gene isolation, Techniques of DNA sequencing, Artificial
DNA synthesis

LESSON PLAN FOR THEORY COURSES (THEORY AND TUTORIAL CLASSES)

FOR THEORY 15 weeks * 4 Hours = 60 Classes (1 credit = 1 Lecture Hour)
L- Topic for Delivery Tutorial/ Skill Competency
No Practical
Plan
History, Scope of genetic
1 engineering
2 Restriction enzymes
3 Restriction enzymes
4 Restriction enzymes
5 Modifying enzymes
6 DNA ligase
7 Polymerase etc.
8 Polymerase Chain
Reaction
9 Basic principles
modification and
applications of PCR
Technique
10 Basic principles
modification and
applications of PCR
Technique
11 Basic principles

COURSEPACK | FORMAT
 modification and
applications of PCR
Technique
12 Various types of PCR
techniques
13 Various types of PCR
techniques
14 Various types of PCR
techniques
15 Various types of PCR
techniques
16 Cloning vehicles
17 Plasmids
18 Lambda phage
19 Phagemids
20 Cosmids
21 Artificial chromosomes
(BACs, YACs)
22 Shuttle vectors
23 virus based vectors
24 Molecular Probes
25 DNA probes, RNA probes
26 Radioactive labeling,
Non-radioactive labeling
27 use of molecular probes
28 DNA fingerprinting
29 Transformation,
transduction
30 Particle gun,
Electroporation
31 liposome mediated,
microinjection
32 Agrobacterium mediated
gene transfer
33 Expression vectors,
Restriction enzyme
analysis
34 Southern blotting,
Northern blotting
35 Western blotting
36 In-situ hybridization.
Colony and plaque
hybridization
37 Factors affecting
expression of cloned
genes
38 Reporter genes, Fusion
proteins
39 cDNA synthesis
40 cDNA libraries
41 Genomic DNA libraries

COURSEPACK | FORMAT
 42 Amplification of gene
libraries
43 Identifying the products
of cDNA clones
44 Techniques of DNA
sequencing, Artificial
DNA synthesis

BIBLIOGRAPHY
Text Books:
1. From Genes to Clones by Winnacker. PANIMA
2. Molecular Biotechnology by Pasternack and Glick.
3. From Genes to Genomes: Concepts & Applications of DNA Technology by J.W. Dale & M.V.
Schartz.
4. Gene Cloning & DNA Analysis: An Introduction (4th edition) by T.A. Brown.
5. Molecular Cloning by Sambrook, et al.
6. Principles of Gene Cloning by Old and Primrose.
Reference Book (s)
1. Molecular Cloning by Sambrook, et al.
2. Principles of Gene Cloning by Old and Primrose.

Journals/Magazines/Govt. Reports/Gazatte/Industry Trends

PROBLEM-BASED LEARNING
Exercises in Problem-based Learning (Assignments)
S. No. Problem KL
1 K2
2 K4
3 K4
4 K3
5 K4
6 K3
7 K4
8 K4
9 K5
10 K5
11 K5
12 K4

COURSEPACK | FORMAT
 13 K5
14 K4
15 K3
16 K5
17 K3

18 K4
19 K5
20 K4
21 K5
22 K4
23 K5
24 K4
25 K5

SELF-LEARNING THROUGH MOOCs (Cognitive Skills): SWAYAM/NPTEL/MOOCs

1. https://onlinecourses.swayam2.ac.in/ugc19_bt16/preview
2. https://ugcmoocs.inflibnet.ac.in/index.php/courses/view_ug/141
3. https://archive.nptel.ac.in/courses/102/107/102107028/

Webliography (Two electronic documents or websites that relate to the Course)

1. https://www.tutorialspoint.com/analytical-techniques-in-biotechnology
2. https://scialert.net/fulltext/?doi=ajbmb.2014.1.7

COURSEPACK | FORMAT
Name of The Course Recombinant DNA Technology Tools &Techniques
Course Code B020301T
Prerequisite Higher Secondary Examination with Chemistry and Biology or
Chemistry, Botany and Zoology or Biochemistry and Chemistry from
a recognized Board in science stream with a minimum of 50 % marks
in aggregate
Corequisite Basic knowledge of physiology.
Antirequisite
L T P C
6 0 0 6

Course Objectives: Students are able to understand the basic concept of Physiology and they
can apply the knowledge of physiology in understanding the various diseases and keeping the
body in healthy state.

Course Outcomes

CO1 Basic concepts in genetic engineering

CO2 Tools of Genetic Engineering and Cloning Vectors
CO3 Molecular Probes and Method of Gene Transfer
CO4 Analysis and Expression of gene in host cells
CO5 Gene Libraries, Isolation, Sequencing and Synthesis of genes
CO6 Polymerase Chain Reaction

Continuous Assessment Pattern

Internal Assessment (IA) Mid Term Test (MTE) End Term Test (ETE) Total Marks
20 30 50 100

Course Content:

COURSEPACK | FORMAT
Unit 1 - Basic concepts and Tools of Genetic Engineering: Enzymes 15 Hours
Basic terminologies and concept, History, Scope and Future; Restriction enzymes, Modifying enzymes, DNA
ligase, Polymerase etc.
Unit-2: Polymerase Chain Reaction 15 Hours
Basic principles modification and applications of PCR Technique, Various types of PCR techniques and
mutagenesis
Unit-3 Tools of Genetic Engineering: Cloning Vectors 15 Hours
Cloning vehicles, Plasmids, Lambda- phage, Phagemids, Cosmids, Artificial chromosomes (BACs, YACs),
Shuttle vectors, virus-based vectors.
Unit -4 Molecular Probes and Method of Gene Transfer 15 Hours
DNA probes, RNA probes, Radioactive labeling, non- radioactive labeling, use of molecular probes, DNA
fingerprinting, Transformation, transduction, Particle gun, Electroporation, liposome mediated, microinjection,
Agrobacterium mediated gene transfer
Unit -5 Analysis and Expression of gene in host cells 15 Hours
Expression vectors, Restriction enzyme analysis, Southern blotting, Northern blotting, Western blotting, In-situ
hybridization. Colony and plaque hybridization, Factors affecting expression of cloned genes, Reporter
genes, Fusion proteins.
Unit-6 Gene Libraries, Isolation, Sequencing and Synthesis of genes 15 Hours
cDNA synthesis, Genomic DNA libraries, Amplification of gene libraries, Identifying the products of cDNA
clones. Different methods of gene isolation, Techniques of DNA sequencing, Artificial DNA synthesis

COURSEPACK | FORMAT
Suggested Readings
1. From Genes to Clones by Winnacker. PANIMA
2. Molecular Biotechnology by Pasternack and Glick.
3. From Genes to Genomes: Concepts & Applications of DNA Technology by J.W. Dale & M.V.
Schartz.
4. Gene Cloning & DNA Analysis: An Introduction (4th edition) by T.A. Brown.
5. Molecular Cloning by Sambrook, et al.
6. Principles of Gene Cloning by Old and Primrose.

Continuous Assessment Pattern

Internal Assessment (IA) CAT End Term Test (ETE) Total Marks
20 30 50 100

COURSEPACK | FORMAT