Mr. Philip Genare E.

Sanchez
 • The learners should be able to outline the
steps involved in genetic engineering
STEM_BIO11/12-III a-b-6
• Discuss the applications of recombinant DNA
STEM_BIO11/12- IIIa-b-7
 Targets for today:
At the end of the lesson, the students should be able to:

Participate in group Develop an appreciation

Identify key for the responsibility
differences discussions and
associated with
collaborative
between classical manipulating genetic
activities to foster material and its impact
breeding methods teamwork and on the environment,
and modern genetic communication skills. agriculture, and human
engineering health.
techniques.
Reminiscing about
the past…
 What is protein
synthesis, and explain its
process?
Genetic engineering involves the use of molecular
techniques to modify the traits of a target
organism.
The modification of traits may involve:

1. introduction of new traits into an organism

2. enhancement of a present trait by increasing the
expression of the desired gene
3. enhancement of a present trait by disrupting the
inhibition of the desired genes’ expression.
1. Cutting or Cleavage of DNA by Restriction Enzymes (REs)
2. Selection of an Appropriate Vector
3. Ligation (Joining) of the Gene of Interest with the
Vector
4. Transfer of the Recombinant Plasmid into a Host Cell
5. Selection Process to Screen Cells Containing the Gene of
Interest
6. Sequencing of the Gene to Determine Primary Structure
of the Protein
 Recombinant DNA Methods

Microprojectile
bombardment
Use of Electroporation
microbial
vectors
 Use of Microbial
vector
often referred to as a
"cloning vector," is a small
DNA molecule that is used to
carry foreign DNA into a
microbial host organism, such
as bacteria or yeast.
 Microprojectile bombardment
In this “biolistic”
technique, a
“gene gun” is
used to fire
DNA-coated
pellets on
tissues.
 Electroporation
This technique expands the membrane pores
through an electric “shock”.
 Methods of Genetic engineering:

Cloning Gene
splicing

Polymerase chain reaction CRISPR-Cas9

Methods of Genetic engineering:

Cloning Gene
splicing

Polymerase chain CRISPR-Cas9

reaction
 Methods of Genetic engineering:

Cloning Gene
splicing

Polymerase chain reaction CRISPR-Cas9

Methods of Genetic engineering:

Cloning Gene
splicing

Polymerase chain CRISPR-Cas9

reaction
Methods of Genetic engineering:

Cloning Gene
splicing

Polymerase chain CRISPR-Cas9

reaction
1. What is protein synthesis, and explain
its process?
2. What is the difference between
Transcription and Translation?
3. What is Genetic Engineering?
4. How is DNA Replication related to
Genetic Engineering?
5. How is DNA Replication related to
Protein Synthesis?
Designer Genes Work
1. How does DNA Replicate?
2. What is a Genetically Modified Organism
(GMO)?
3. Illustrate your own Designer genes
based on the following:
1. Identify a special trait.
2. Identify a source organism.
3. Identify a target organism.
4. Identify the modified/ added trait.