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    1) The document summarizes the process of eukaryotic ribosome biogenesis in three stages: nucleolar pre-60S biogenesis, nucleoplasmic pre-60S biogenesis, and cytoplasmic pre-60S maturation. 2) In the nucleolus, RNA polymerase I transcribes the 35S pre-rRNA which is processed and assembled with ribosomal proteins and trans-acting factors to form the pre-60S particle. 3) The pre-60S particle then undergoes further processing and remodeling in the nucleoplasm before being exported into the cytoplasm through the nuclear pore complex. 4) In the cytoplasm, the pre-60S undergoes final maturation

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    Ribosome assembly

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    Ribosome assembly

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    1) The document summarizes the process of eukaryotic ribosome biogenesis in three stages: nucleolar pre-60S biogenesis, nucleoplasmic pre-60S biogenesis, and cytoplasmic pre-60S maturation. 2) In the nucleolus, RNA polymerase I transcribes the 35S pre-rRNA which is processed and assembled with ribosomal proteins and trans-acting factors to form the pre-60S particle. 3) The pre-60S particle then undergoes further processing and remodeling in the nucleoplasm before being exported into the cytoplasm through the nuclear pore complex. 4) In the cytoplasm, the pre-60S undergoes final maturation

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    © All Rights Reserved
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    2 views1 page

    Ribosome Assembly

    Uploaded by

    cristina hermosillo
    1) The document summarizes the process of eukaryotic ribosome biogenesis in three stages: nucleolar pre-60S biogenesis, nucleoplasmic pre-60S biogenesis, and cytoplasmic pre-60S maturation. 2) In the nucleolus, RNA polymerase I transcribes the 35S pre-rRNA which is processed and assembled with ribosomal proteins and trans-acting factors to form the pre-60S particle. 3) The pre-60S particle then undergoes further processing and remodeling in the nucleoplasm before being exported into the cytoplasm through the nuclear pore complex. 4) In the cytoplasm, the pre-60S undergoes final maturation

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    © All Rights Reserved
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    Eukaryotic ribosome biogenesis II


    Ed Hurt,1 Janet Iwasa,2 and Roland Beckmann3
    1
    Heidelberg University Biochemistry Center (BZH), Heidelberg, Germany; 2Department of Biochemistry, University of Utah, Salt Lake City, UT, USA ; 3Gene Center, Ludwig-
    Maximilians-Universität München, Munich, Germany

    Cleavage sites A0 A1 D A2 A3 B1 C2 C1 B2

    Pol I 35S pre-rRNA 5v 18S 5.8S 25S 3v Pol III 5S


    ITS1 ITS2

    5S rRNA transcription
    NUCLEOLAR PRE-60S BIOGENESIS

    Pol I Earliest 5S RNP Pol III


    Nucleolar +ATP
    pre-60S Foot factors factors
    25S 5S Rea1
    factors factors
    ITS2 RNA
    Has1 5v uL18
    5S RNP uL5 Ytm1-Erb1
    5.8S
    ITS1 Brx1- assembly
    5v 18S Noc1- Spb4
    Noc2 Ebp2 Noc2-Noc3
    Nucleolar
    Pre-rRNA H84
    A2 A2 factors 5S
    transcription Spb1
    Rrp5 cleavage Dbp10 RNA
    snoRNPs
    Earliest 90S Rlp24 Nog1
    factors Further
    90S Tif6
    factors 90S
    90S-pre-60S Pre-60S (state A) Pre-60S (Dbp10-3) Pre-60S (state E)
    particle Pre-40S biogenesis

    Pre-60S 7S>5.8S
    Rixosome
    (nuclear Nmd3) processing
    +ATP 5S RNP ITS2 Exosome
    Rea1 rotated Mtr4
    and Rix1 5S RNA
    complex rotation AIM
    NES Nop53 Nop53
    Nmd3
    90˚
    Nog2
    Arx1
    Rsa4 Bud20
    Exosome
    +ATP

    Nuclear export
    Export Pre-60S Pre-60S Pre-60S Pre-60S
    factors
    (Rea1-Rix1) (Nop53 exosome Mtr4) (Nog2/Arx1) (NE2)
    NPC NUCLEOPLASM NUCLEOPLASMIC PRE-60S BIOGENESIS

    CYTOPLASM C Y T O P L A S M I C P R E - 6 0 S M AT U R AT I O N
    Central
    protuberance
    Crm1- Mrt4
    L1 P-stalk
    RanGTP 5S
    Nog1 uL16 stalk
    Sdo1 Efl1
    Yvh1 5.8S PTC
    Lsg1
    Rlp24 Tif6
    Mex67 eL24 25S rRNA
    -Mtr2 18S
    PET rRNA
    Drg1
    60S 40S
    Pre-60S in NPC Pre-60S (Drg1) Pre-60S (Lsg1) Pre-60S (Efl1) subunit subunit

    Pre-60S assembly factors


    Earliest pre-60S factors snoRNPs Nucleolar factors Foot factors Nuclear factors Cytoplasmic
    Noc1 Dbp6 H/ACA Cbf5 Nhp2 Rrp1 Ssf1 Tif6 Rix7 Cic1 Rea1 Nog2 factors
    Noc2 Upa1 snR35 Gar1 Nop10 Nsa1 Ssf2 Nop2 Dbp3 Nop15 Rix1 Cgr1 Drg1
    Rrp5 Upa2 C/D box Nop1 Nop56 Rpf1 Rrp15 Nip7 Dbp7 Rlp7 Ipi3 Rsa4 Yvh1
    Urb1 Mak5 snR190 Snu13 Nop58 Mak16 Rrp14 Nog1 Dpb9 Nop7 Ipi1 Nop53 Rei1
    Urb2 Rbp95 Brx1 Nop16 Mak11 Dbp10 Export factors Sda1 Arx1 Reh1
    Noc2 Nop4 5S RNP Ebp2 Spb4 Has1 Drs1 Crm1 Las1 Alb1 Lsg1
    Noc3 Pwp1 5S RNA Syo1 Ytm1 Puf6 Nsa2 Mrt4 RanGTP Grc3 Bud20 Sdo1
    Rsa3 Nop12 uL18 Rpf2 Erb1 Loc1 Spb1 Mex67 Rat1 Ecm1 Efl1
    Nop8 Nop13 uL5 Rrs1 Mak15 Rlp24 Mtr2 Rai1 Nmd3

    Scenes from the video Eukaryotic pre-60S ribosome biogenesis

    90S-pre-60S assembly Nucleolar Nuclear Nuclear Pre-60S Pre-60S Cytoplasmic Mature 60S
    pre-60S pre-60S with pre-60S with in NPC following pre-60S subunit
    Mtr4-exosome Rea1-Rixosome nuclear export

    See online version for supplemental information,


    1314 Cell 187, February 29, 2024 © 2024 Elsevier Inc. DOI https://doi.org/10.1016/j.cell.2024.01.043 legends, references, and declaration of interests

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