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    Department of Biotechnology

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    CuriOus SpaRk
    The document discusses Matrix Assisted Laser Desorption/Ionization (MALDI), a versatile analytical technique used to detect and characterize mixtures of organic molecules like peptides, lipids, and saccharides. MALDI uses a laser to ionize analyte molecules that have been mixed with an absorbing matrix. The ionized analyte molecules are then accelerated into a time-of-flight mass spectrometer which generates mass spectra that can be used to identify molecules based on mass. Variations of MALDI like MALDI-TOF are commonly used to analyze proteins and peptides and provide fast, sensitive analysis with very small sample amounts.

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    Department of Biotechnology

    Uploaded by

    CuriOus SpaRk
    2 views9 pages
    The document discusses Matrix Assisted Laser Desorption/Ionization (MALDI), a versatile analytical technique used to detect and characterize mixtures of organic molecules like peptides, lipids, and saccharides. MALDI uses a laser to ionize analyte molecules that have been mixed with an absorbing matrix. The ionized analyte molecules are then accelerated into a time-of-flight mass spectrometer which generates mass spectra that can be used to identify molecules based on mass. Variations of MALDI like MALDI-TOF are commonly used to analyze proteins and peptides and provide fast, sensitive analysis with very small sample amounts.

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    BIOTECHNOLOGY

    Original Title

    DEPARTMENT OF BIOTECHNOLOGY (1)

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    The document discusses Matrix Assisted Laser Desorption/Ionization (MALDI), a versatile analytical technique used to detect and characterize mixtures of organic molecules like peptides, lipids, and saccharides. MALDI uses a laser to ionize analyte molecules that have been mixed with an absorbing matrix. The ionized analyte molecules are then accelerated into a time-of-flight mass spectrometer which generates mass spectra that can be used to identify molecules based on mass. Variations of MALDI like MALDI-TOF are commonly used to analyze proteins and peptides and provide fast, sensitive analysis with very small sample amounts.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    2 views9 pages

    Department of Biotechnology

    Uploaded by

    CuriOus SpaRk
    The document discusses Matrix Assisted Laser Desorption/Ionization (MALDI), a versatile analytical technique used to detect and characterize mixtures of organic molecules like peptides, lipids, and saccharides. MALDI uses a laser to ionize analyte molecules that have been mixed with an absorbing matrix. The ionized analyte molecules are then accelerated into a time-of-flight mass spectrometer which generates mass spectra that can be used to identify molecules based on mass. Variations of MALDI like MALDI-TOF are commonly used to analyze proteins and peptides and provide fast, sensitive analysis with very small sample amounts.

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    © All Rights Reserved
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    of 9

    DEPARTMENT OF BIOTECHNOLOGY

    Subject -- Instrumentation

    Topic - Matrix Assisted laser Ionization(MALDI)

    Submitted To -- Submitted BY --
    Miss . Radha Kaushik Mam Deepanshu
    INTRODUCTION
    ✓ MALDI is the abbreviation for “Matrix Assisted
    Laser desorption/Ionization.”

    ✓ MALDI is appropriated to analyze biomolecules like


    peptides, lipids, saccharides, or other organic
    macromolecules.

    ✓ TOF stands for “Time of Flight”.

    ✓ This generates characteristic mass spectral


    fingerprints which are compared with large library
    of mass spectra.

    ✓ A versatile analytical technique to detect and


    characterize mixtures of organic molecules.
    Principle of MALDI

    ➢ Matrix absorbs the ultraviolet light (nitrogen laser light,


    wavelength 337 nm) and converts it to heat energy.

    ➢ The analyte is embedded on the Target Plate.

    ➢ After a laser pulse, the irritated spot is rapidly heated and


    becomes vibrationally excited.

    ➢ Matrix molecules energetically ablated from the surface of


    the sample.

    ➢ During ablation, the analyte molecules are usually ionized


    by being protonated or deprotonated with the nearby
    matrix molecules.
    TYPES OF MALDI VARIATION
    ➢ It may involve the absorption of light by the matrix .

    ➢ Transfer of this energy to the analyte –

    • Which then ionizes into the gas phase as a result of


    the relatively large amount of energy absorbed.

    • To accelerating the resulting ions into a flight-tube


    in the mass spectrometer they are subjected to a
    high electric field.
    ❖ Used for the analysis of proteins and peptides.
    Changes in structure can also be identified.

    ❖ Very Low amount of sample. Sample preparation is


    easy and a spectrum is simple.

    ❖ Very fast separation method.

    ❖ Can be used to improve the diagnosis of infections


    produced by viruses. It can also help discover
    mutations and variants of these microorganisms and
    detect antiviral resistance.

    ❖ Rapidly determine a mass pattern of proteins. It can


    also identify specific protein markers and peptide
    sequence variations among assessed species.

    ❖ Rapidly determine a mass pattern of proteins. It can


    also identify specific protein markers and peptide
    sequence variations among assessed species.

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