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Ik Engl
Ik Engl
Retention Index
Prof. Dr. Eduardo Miranda Ethur
Universidade do Vale do Taquari
Univates
Kovats retention index formula:
Where:
• RI = retention index of “i”.
• i = constituent of essential oil that is being analyzed.
• n = carbon number of the alkane which elutes before “i”.
• m = number of carbons of the alkane which elutes after “i”.
• tri = retention time of “i”.
• trn = retention time of the alkane which elutes before “i”.
• trm = retention time of the alkane which elutes after “i”.
Inject the Essential Oil sample in appropriated
columns and conditions.
Chromatogram (1).
Inject a series of hydrocarbon (in this case from C8
to C30), at the same column and condition.
Chromatogram (2).
C30
C25
C20
C10 C15
For this example we select the range of 4 to 15
minutes of the chromatogram (1).
.
C30
C25
C20
C10 C15
Expansion of the chromatogram (2), between 4
and 15 minutes.
C9
C10 C11 C12
Comparison (software or co-injection) of the
mixture of n-alkanes and essential oil, in the region
of 4 to 15 minutes, and selection of "i".
i?
n
C9 m
C10
Experimental Data
= 978
Option 1
Option 2
Comparing the retention index of Kovats with
the mass spectrum we have:
Experimental RI = 978
Experimental MS
Both MS and RI
correspond to the
b-Pinene
Thanks!
eduardome@univates.br