TABLE OF CONTENT

DECLARATION…….……………………………………………………………………………i
CHAPTER ONE ............................................................................................................................. 1

INTRODUCTION .......................................................................................................................... 1

1.1 Background Information ....................................................................................................... 1

1.2 Statement of the problem ...................................................................................................... 5

1.3 Objectives of the study .......................................................................................................... 5

1.3.1 General Objectives ......................................................................................................... 5

1.3.2 Specific objectives .......................................................................................................... 5

1.3.3 Research Questions......................................................................................................... 5

1.4 Significance of the study ....................................................................................................... 5

CHAPTER TWO ............................................................................................................................ 6

LITERATURE REVIEW............................................................................................................ 6

2.1 Effect geographical location of pineapple. ............................................................................ 6

2.2 Method of purification of bromelain ..................................................................................... 7

CHAPTER THREE ........................................................................................................................ 8

METHODOLOGY ......................................................................................................................... 8

3.1 Study area and sample sollection....................................................................................... 8

3.2 Study design ..................................................................................................................... 8

3.3 Materials ............................................................................................................................ 8

3.4 Reagents and chemicals ..................................................................................................... 8

3.5 Measurements Procedures ................................................................................................. 8

ESTIMATED BUDGET ................................................................................................................. 9

TIME FRAME .............................................................................................................................. 10

REFERENCES ............................................................................................................................. 11